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Microbial Activity of Gravel Intertidal Zone for Purification of Polluted Near Shore Water

  • Song, Young-Chae;Gu, Ja-Hwan;Park, In-Seok;Yoo, Jong-Su
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2004.04a
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    • pp.233-239
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    • 2004
  • Microbial activity of biofilm formed on the surface of gravels from intertidal zone was estimated using an aerobic respirometer system, and compared with that of suspended marine microorganisms contained in a near shore water. The maximum oxygen uptake rate of the suspended marine microorganisms was 0.15mg O$_2$/L/hr, indicating the potential of purification of polluted near shore water. For the gravels from the intertidal zone, the maximum uptake rate of oxygen was affected by the vertical positions, but their gross value was 0.77mg O$_2$/L/hr, which was around 5.1 times higher than the purification potential of polluted near shore water by the microorganisms contained in the near shore water. The nitrogen removed by the gravels from the intertidal zone and the marine microorganisms was about 1/20-1/39 times of the total consumption of oxygen, which was similar to that of the phosphate. The gravel intertidal zone contained lots of particulate organics, over than that in the near shore water, and this was confirmed from the large difference between total oxygen consumption and the removed soluble COD in the microbial activity test. This indicates that the gravel intertidal zone plays an important role in controlling the non-point source pollutants from land, as well as self-purification of polluted near shore water by trapping and degrading the particulate organics.

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A Study on the Appropriate Selection of a Power System Stabilizer and Power Converters for HVDC Linked System (HVDC 연계 시스템의 전력계통 안정화 장치와 전력변환기의 적정 파라메터 선정에 관한 연구)

  • 김경철;문병희
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.16 no.2
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    • pp.45-53
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    • 2002
  • This paper presents an algorithm for the appropriate parameter selection of a power system stabilizer and power converters in two-area power systems with a series HVDC links. The method for PSS is one of the classical techniques by allocating properly poly-zero positions to fit as closely as desired the ideal phase lead and by changing the gain to produce a necessary damping torque. Proper parameter of power converters are obtained in order to have sufficient speed and stability margin to cope with changing reference values and disturbances based on the Root-locus technique. The small signal and transient stability studies using the PSS and power converters parameters obtained from these methods show that a natural oscillation frequency of the study case system is adequately damped. The simulation used in the paper was performed by the Power System Toolbox software program based on MATLAB.

Marker-Assisted Foreground and Background Selection of Near Isogenic Lines for Bacterial Leaf Pustule Resistant Gene in Soybean

  • Kim, Kil-Hyun;Kim, Moon-Young;Van, Kyu-Jung;Moon, Jung-Kyung;Kim, Dong-Hyun;Lee, Suk-Ha
    • Journal of Crop Science and Biotechnology
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    • v.11 no.4
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    • pp.263-268
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    • 2008
  • Bacterial leaf pustule (BLP) caused by Xanthomonas axonopodis pv. glycines is a serious disease to make pustule and chlorotic haloes in soybean [Glycine max (L). Merr.]. While inheritance mode and map positions of the BLP resistance gene, rxp are known, no sequence information of the gene was reported. In this study, we made five near isogenic lines (NILs) from separate backcrosses (BCs) of BLP-susceptible Hwangkeumkong $\times$ BLP-resistant SS2-2 (HS) and BLP-susceptible Taekwangkong$\times$ SS2-2 (TS) through foreground and background selection based on the four-stage selection strategy. First, 15 BC individuals were selected through foreground selection using the simple sequence repeat (SSR) markers Satt486 and Satt372 flanking the rxp gene. Among them, 11 BC plants showed the BLP-resistant response. The HS and TS lines chosen in foreground selection were again screened by background selection using 118 and 90 SSR markers across all chromosomes, respectively. Eventually, five individuals showing greater than 90% recurrent parent genome content were selected in both HS and TS lines. These NILs will be a unique biological material to characterize the rxp gene.

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A Study on the Improvement Methods on Cabin Safety Actions in Aircraft Accidents -Focused on Cabin Crew Safety Performances & Passenger Perceptions of Cabin Safety Information - (항공기 비상사태에 대한 객실안전조치에 관한 연구 -객실승무원의 안전업무와 승객의 객실안전정보 인식을 중심으로-)

  • Yoo, Kyung-In;Yoo, Kwang-Eui;Lee, Chun-Ki
    • Journal of the Korean Society for Aviation and Aeronautics
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    • v.21 no.4
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    • pp.126-136
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    • 2013
  • Aircraft accidents these days, following the mega sizing trend of the aircraft, result in enormous losses of human lives apart from those of property, which cannot be replaced by any means. As most recently, in April 20, 2012, a Boeing 737 passenger plane departed Karachi on an augural flight to Islamabad, Pakistan, crashed close to an express highway on final approach, all 121 passengers and 6 crew members were killed. As such a large number of fatalities have been recorded in aircraft accidents while accident investigation results show that more than 95% of aircraft accidents are now survivable. There are three basic stages in surviving the aircraft accident: surviving the crash impact, the evacuation process and the hostile post evacuation environmental elements. These stages require the cabin crew's expeditious and appropriate actions on the basis of systematic and thorough cabin safety training in order to increase occupants' survivability, along with the passengers' preparedness. In this aspect, this paper examines the issues acting as the impediments to the passenger survival in inflight emergency situations, that are the deficiencies with cabin crew safety training, related performances and the shortcomings in passengers' knowledge on inflight safety information, leading to their inappropriate responses to emergency situations. These issues are analyzed and the root causes are identified, suggesting the resolving countermeasures.

Development of Autonomous Combine Using DGPS and Machine Vision (DGPS와 기계시각을 이용한 자율주행 콤바인의 개발)

  • Cho, S. I.;Park, Y. S.;Choi, C. H.;Hwang, H.;Kim, M. L.
    • Journal of Biosystems Engineering
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    • v.26 no.1
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    • pp.29-38
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    • 2001
  • A navigation system was developed for autonomous guidance of a combine. It consisted of a DGPS, a machine vision system, a gyro sensor and an ultrasonic sensor. For an autonomous operation of the combine, target points were determined at first. Secondly, heading angle and offset were calculated by comparing current positions obtained from the DGPS with the target points. Thirdly, the fuzzy controller decided steering angle by the fuzzy inference that took 3 inputs of heading angle, offset and distance to the bank around the rice field. Finally, the hydraulic system was actuated for the combine steering. In the case of the misbehavior of the DGPS, the machine vision system found the desired travel path. In this way, the combine traveled straight paths to the traget point and then turned to the next target point. The gyro sensor was used to check the turning angle. The autonomous combine traveled within 31.11cm deviation(RMS) on the straight paths and harvested up to 96% of the whole rice field. The field experiments proved a possibility of autonomous harvesting. Improvement of the DGPS accuracy should be studied further by compensation variations of combines attitude due to unevenness of the rice field.

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Measurement of Worker's Physiological and Biomechanical Responses during the Cherry Tomato Harvesting Work in a Greenhouse (온실에서 방울토마토 수확작업시 작업자의 생리학적 및 생체역학적 반응 측정)

  • SeonWoo, Hoon;Lim, Ki-Taek;Kim, Jang-Ho;Son, Hyun-Mok;Chung, Jong-Hoon
    • Journal of Biosystems Engineering
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    • v.36 no.3
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    • pp.223-230
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    • 2011
  • Physiological signals such as body temperature, heart rate, blood pressure and heart rate variability and biomechanical workload for stress analysis were investigated during the cherry tomato harvesting work in a greenhouse. The skin temperatures raised $0.05^{\circ}C$/min, $0.03^{\circ}C$/ min, and $0.08^{\circ}C$/min in standing, stooping and squatting postures, respectively. Breath rate significantly increased from 18 to 28 breaths/min during the cherry tomato harvesting work. As the heart rate during the work ranged from about 72 to 110 beats/min (bpm), the cherry tomato harvesting work appeared to be a light intensity task of less than 110 bpm. The worker's average energy consumption rate in three positions during 43 min working time was 65.74 kcal (91 kcal/h in 70 kg). This was a light intensity of work, compared to 75 kcal/h in 70 kg of basic metabolic energy consumption rate of a worker with 70 kg weight; The maximum shear force on the disk (L5/ S1) due to static workload in the cherry tomato harvesting work was 446 N in the stooping posture, 321 N in the squatting posture and 287 N in the standing posture. Acute stress index expressed with the heart rate variability, increased parasympathetic activation up to about 70 while workers were doing most agricultural work in this study. This study provided a system to measure quantitatively workers' physiological change, kinematics and kinetic factors without any restrictions of space in the greenhouse works.

Analysis of the Cutting Shape as a Function of Feed Rate and Cutting Speed of Korean and Japanese Combines

  • Jin, Byung-Ok;Lee, Min-Ho;Jo, Jin-Seok;Jung, Ho-Jun;Kim, Chi-Ho;Kim, Hyeon-Tae
    • Journal of Biosystems Engineering
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    • v.42 no.2
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    • pp.80-85
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    • 2017
  • Purpose: In this study, we attempted to analyze, by using a high-speed camera, the cutting shape as a function of cutting speed and feed rate. We compared the differences in cutting shape between domestic and foreign combines. Methods: Experiments were performed using plastic straws, and the results of two combine cutting blades, one from the Daedong Industry and one from Kuboda, were compared. The quality and performances of cutting were measured at three cutting positions: center and 68 cm to the left and right of the center. The feed rates were 0.6 m/s, 1.1 m/s, 1.6 m/s, and the cutting speeds were 600 RPM, 990 RPM, 1,380 RPM. For each speed, the cutting shape was measured three times, and the entire procedure was also repeated three times. Results: In the experiments, the domestic cutting blade achieved better results than the Japanese cutting blade. These results were obtained by studying the combination of feed rate and cutting speed, with the domestic combine attaining approximately 80% performance of the Japanese combine. We believe that additional data analysis is required, obtained from field experiments. Conclusions: The domestic cutting knives achieved better results than the Japanese cutting knives. These results are estimated from experiments conducted with different feed rates and cutting speeds; an in-depth analysis will require experiments in the real field with actual combines and a combination of multiple variables. Repeating the investigation on the length differences, broken and cut angle with various combinations of feed rate and cutting speed, will surely help to find the optimal cutting speed.

Analysis System for Practical Dynamic Load with Hybrid Method under Random Frequency Vibration (불규칙 가진시 하이브리드기법을 이용한 실동하중 해석시스템)

  • Song, Joon-Hyuk;Yang, Sung-Mo;Kang, Hee-Yong;Yu, Hyo-Sun
    • Transactions of the Korean Society of Automotive Engineers
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    • v.16 no.6
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    • pp.33-38
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    • 2008
  • Most structures of vehicle are composed of many substructures connected to one another by various types of mechanical joints. In vehicle engineering, it is important to study these jointed structures under random frequency vibration for the evaluations of fatigue life and stress concentration exactly. It is rarely obtained the accurate load history of specified positions in a jointed structure because of the errors such as modeling, measurement, and etc. In the beginning of design, exact load data are actually necessary for the fatigue strength and life analysis to minimize the cost and time of designing. In this paper, the hybrid method of practical dynamic load determination is developed by the combination of the principal stresses from F. E. Analysis and test of a jointed structure. Least square pseudo inverse matrix is adopted to obtain an inverse matrix of analyzed stresses matrix. The error minimization method utilizes the inaccurate measured error and the shifting error that the whole data is stiffed over real data. The least square criterion is adopted to avoid these errors. Finally, to verify the proposed system, a heavy-duty bus is analyzed. This measurement and prediction technology can be extended to the different jointed structures.

Study on Evaluation Method of Flow Characteristics in Steady Flow Bench(5)-Effect of Evaluation Position (정상유동 장치에서 유동 특성 평가 방법에 대한 연구(5) - 평가위치의 영향)

  • Cho, Siehyung;Ohm, Inyong
    • Transactions of the Korean Society of Automotive Engineers
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    • v.25 no.2
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    • pp.179-189
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    • 2017
  • This paper is the fifth investigation on the methods of evaluating flow characteristics in a steady flow bench. In previous studies, several assumptions used in the steady flow bench were examined and it was concluded that the assumption of the solid rotation may lead to serious problems. In addition, though the velocity profiles were improved as the measuring position went downstream, the distributions were far from ideal regardless of the valve angle and evaluation position. The eccentricities were also not sufficiently small to disregard the effect on impulse swirl meter (ISM) measurement. Therefore, the effect of these distribution and eccentricity changes according to the positions needs to be analyzed to discuss the method of flow characteristics estimation. In this context, the effects of evaluation position on the steady flow characteristics were studied. For this purpose, the swirl coefficient and swirl ratio were assessed and compared via measurement of the conventional ISM and calculation based on the velocity by particle image velocimetry(PIV) from 1.75B, 1.75 times bore position apart from the cylinder head, to the 6.00B position. The results show that the swirl coefficients by ISM strictly decrease and the curves as a function of the valve lift become smooth and linear as the measuring position goes downstream. However, the values through the calculation based on the PIV are higher at the farther position due to the approach of the tangential velocity profile to ideal. In addition, there exists an offset effect between the velocity distribution and eccentricity in the low valve lift range when the coefficients are estimated based on the swirl center. Finally, the curve of the swirl ratio by ISM and by PIV evaluation as a function the measuring position intersect around 5.00B plane except at $26^{\circ}$ valve angle.

Expression and Purification of Mutated Porcine Growth Hormone Binding Protein by Using Site-Directed Mutagenesis in E. coli (Site-Directed Mutagenesis를 이용하여 변이된 돼지 성장 호르몬 결합 단백질의 대장균 내 발현과 정제)

  • Choi, K.H.;Chung, K. S.;Lee, H.T.
    • Korean Journal of Animal Reproduction
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    • v.25 no.4
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    • pp.381-388
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    • 2001
  • The present study was designed to obtain porcine growth hormone binding protein (pGHBP) improved biological activation as derived mutation in binding site with growth horlnone (GH). A 756 bp of fragment encoding the extracellular domain of pGHBP gene was cloned from the total RNA of porcine fat tissue by reverse transcriptase polymerase chain reaction (RT-PCR) and created mutation in positions 26 and 122 using site-directed mutagenesis method. Position 26 is one and it is near to get on five potential N-linked glycosylation sites located in the extracellular domain of porcine growth hormone receptor known to have a direct influence on combination with GH. Position 122 is known as one of conformational epitope in bovine. It was over-expressed in E. coli using pET-32(c) expression vector and precisely purified by S-protein agarose and enterokinase. In our results, we was obtained pmGHBP of 30 kDa. It suggests to study the effects of the pmGHBP on cell proliferation in vitro and growth rate in vivo after administration.

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