• BMB Reports
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• 제39권6호
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• pp.709-716
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• 2006

Mammalian polo-like kinase 1 (Plk1) acts at various stages in early and late mitosis. Plk1 localizes at the centrosome and maintains this position through mitosis. Thereafter Plk1 moves to the kinetochore and midbody region, important sites during chromosome separation and cytokinesis. The catalytic domain of Plk1 is in the N-terminus region, whereas the non-catalytic region in the C-terminus of Plk1 has a conserved motif, named the Polobox. This motif is critical for Plk localization. EGFP proteins fused with the N-terminus and C-terminus of Plk1 localize in the nucleus and centrosomes, respectively. The core sequences of the polo-box (50 amino acids) also localize in Plk1 target organelles. To screen for domain-specific target proteins of Plk1, we constructed an N-terminal domain and a tandem repeat polo-box motif, and used them as templates in a yeast two-hybrid screen. The HeLa cell cDNA library indicated several proteins including the centrosome/kinetochore components or regulators, to be characterized as positive clones. Through in vitro protein binding analyses, we confirmed an interaction between these proteins and Plk1. The data reported from this study indicate that the N- and C- termini of Plk1 may function through recruitment and/or activation of domain-specific target proteins in dividing cells. Additionally, tandem repeats of the conserved core motif of the polo-box are sufficient for targeting and may be useful as a centrosome/kinetochore-specific targeting peptide.

• Journal of Korean Society for Geospatial Information Science
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• 제17권4호
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• pp.113-119
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• 2009

In Ubiquitous Information society, real shape of spatial information and accurate position information are required. Spatial information standardization is needed to service for the people. International standard was defined to ensure compatibilities of various standards, technologies, and terminologies and to promote mutual trade certain criteria and standard forms were established through international agreement. International standard is made by International Organization for Standardization, and International Standard Organization Technical Committee 211 manages the geographic information field. ISO TC 211 is working on LADM (Land Administration Domain Model) which defines administration law and spatial surveying component related to land and efficiently manages data and land systems of the countries. This study introduced processing of standardization of a surveying package which is related with LADM and comparing it with Korea Cadastral information system.

• Proceedings of the Korea Crystallographic Association Conference
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• 한국결정학회 2002년도 정기총회 및 추계학술연구발표회
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• pp.4-4
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• 2002

PDZ domains bind to short segments within target proteins in a sequence-specific fashion. GRIP/ABP family proteins contain six to seven PDZ domains and interact via its sixth PDZ domain (class Ⅱ) with the C-termini of various proteins, including liprin-α. In addition the PDZ456 domain mediates the formation of homo- and heteromultimers of GRIP proteins. To better understand the structural basis of peptide recognition by a class Ⅱ PDZ domain and DZ-mediated multimerization, we determined the crystal structures of the GRIPI PDZ6 domain, alone and in complex with a synthetic C-terminal octapeptide of human liprin-α, at resolutions of 1.5 Å and 1.8 Å, respectively. Remarkably, unlike other class Ⅱ PDZ domains, Ile736 at αB5 rather than conserved Leu732 at αB1 makes a direct hydrophobic contact with the side chain of the Tyr at the -2 position of the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight reorientation of helix αB, indicating that the second hydrophobic pocket undergoes a conformational adaptation to accommodate the bulkiness of the Tyr's side chain, and forms an antiparallel dimer through an interface located at a site distal to the peptide-binding groove. This configuration may enable formation of GRIP multimers and efficient clustering of GRIP-binding proteins.

• Journal of the Society of Naval Architects of Korea
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• 제38권3호
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• pp.47-53
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• 2001

When a ship advancing in waves is subjected to impact forces or irregular forces, the motion analyses for ship are convenient for being calculated in the time domain. The added mass, wave damping coefficients, wave exciting forces and mean drift forces are calculated by 3-Dimensional panel method used the translating pulsating Green function in the frequency domain and the motion equations which are considered by the memory effect due to waves are numerically solved by using the Newmark-$\beta$ method in the time domain. The motion analyses are carried out for a Series 60($C_B=0.7$) moving in irregular waves. The items of calculation are 6-degree motions, accelerations at the fore and after position, numbers of deck wetness and numbers of exposure at ship-bottom, etc. Moreover, the thrust addition in waves is examined by considering the time mean drift forces in the motion equations of time domain.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 한국소음진동공학회 2002년도 추계학술대회논문집
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• pp.122-127
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• 2002

A frequency-domain PES estimation and its prediction method are proposed for the tightly-coupled servo/mechanical design of high-TPI HDD system above 100 kTPI. The major two disturbance energies which are related with mechanical vibrations inside of HDD are used to predict the drive-level PES, while considering closed-loop servo dynamics. One is the torque disturbance which mainly comes from aerodynamic excitation of HSA system and the other is the displacement disturbance from disk-spindle dynamics. In order to obtain the accurate error transfer function of closed-loop servo control, the plant model is measured by accurate experiment. The measured PES is compared with predicted one in terms of frequency-domain PES spectrum and its standard variation value. It is proved that the proposed frequency-domain PES estimation/prediction method is capable of predicting drive-level PES of high-TPI hard disk drive.

• Wind and Structures
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• 제31권5호
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• pp.403-418
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• 2020

To investigate the effects of central buckles on the dynamic behavior and flutter stability of long-span suspension bridges, four different connection options between the main cable and the girder near the mid-span position of the Aizhai Bridge were studied. Based on the flutter derivatives obtained from wind tunnel tests, formulations of self-excited forces in the time domain were obtained using a nonlinear least square fitting method and a time-domain flutter analysis was realized. Subsequently, the influences of the central buckles on the critical flutter velocity, flutter frequency, and three-dimensional flutter states of the bridge were investigated. The results show that the central buckles can significantly increase the frequency of the longitudinal floating mode of the bridge and have greater influence on the frequencies of the asymmetric lateral bending mode and asymmetric torsion mode than on that of the symmetric ones. As such, the central buckles have small impact on the critical flutter velocity due to that the flutter mode of the Aizhai Bridge was essentially the symmetric torsion mode coupled with the symmetric vertical mode. However, the central buckles have certain impact on the flutter mode and the three-dimensional flutter states of the bridge. In addition, it is found that the phenomenon of complex beat vibrations (called intermittent flutter phenomenon) appeared in the flutter state of the bridge when the structural damping is 0 or very low.

• Proceedings of the Korea Contents Association Conference
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• 한국콘텐츠학회 2005년도 추계 종합학술대회 논문집
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• pp.480-484
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• 2005

In this paper, the multiwatermarking method to make it possible to the authentication, modification detection and copyright protection of the digital image was proposed. After the original image is transformed by the DWT(Discrete Wavelet Transform), the encrypted watermark information is embedded into the LSB(Least Significant Bit) of LL band on the low frequence domain for authenticating and detecting modified position in the image. And watermark information is embedded by exchanging the wavelet coefficient for the copyright protection. Thereby, we can authenticate the modification and detect the modified location by extracting the values of LSB on the low frequence domain. And we can perceive the copyright information of original image by extracting the embedded watermark on the high frequence domain.

• The Journal of the Korea Contents Association
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• 제6권1호
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• pp.170-178
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• 2006

In this paper, the hybrid-watermarking method to make it possible to the authentication, modification detection and copyright protection of the digital image was proposed. After the original image is transformed by the DWT(Discrete Wavelet Transform), the encrypted watermark information is embedded into the LSB(Least Significant Bit) of LL band on the low frequence domain for authenticating and detecting modified position in the image. And watermark information is embedded by exchanging the wavelet coefficient for the copyright protection. Thereby, we can authenticate the modification and detect the modified location by extracting the values of LSB on the low frequence domain. And we can perceive the copyright information of original image by extracting the embedded watermark on the high frequence domain.

• Proceedings of the Korea Crystallographic Association Conference
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• 한국결정학회 2003년도 춘계학술연구발표회
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• pp.17-17
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• 2003

tRNA (m¹ G37) methyltransferase (TrmD) catalyze s the trans for of a methyl group from S-adenosyl-L-methionine (AdoMet) to G/sup 37/ within a subset of bacterial tRNA species, which have a residue G at 36th position. The modified guanosine is adjacent to and 3' of the anticodon and is essential for the maintenance of the correct reading frame during translation. We have determined the first crystal structure of TrmD from Haemophilus influenzae, as a binary complex with either AdoMet or S-adenosyl-L-homocysteine (AdoHcy), as a ternary complex with AdoHcy/phosphate, and as an apo form. The structure indicates that TrmD functions as a dimer (Figure 1). It also suggests the binding mode of G/sup 36/G/sup 37/ in the active site of TrmD and catalytic mechanism. The N-terminal domain has a trefoil knot, in which AdoMet or AdoHcy is bound in a novel, bent conformation. The C-terminal domain shows a structural similarity to DNA binding domain of trp or tot repressor. We propose a plausible model for the TrmD₂-tRNA₂ complex, which provides insights into recognition of the general tRNA structure by TrmD (Figure 2).

• Journal of the Institute of Electronics Engineers of Korea TC
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• 제37권12호
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• pp.32-37
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• 2000

We proposed to a new method of the aperture coupled microstrip antenna with T-shaped feeding slot. We analyzed method of enhancing the bandwidth of the antenna using FDTD. And the antenna parameters are optimized to get maximum bandwidth. We also calculated the progress process of waves and the distribution of electric field in the time domain. We also calculated return loss, VSWR, input impedance, radiation pattern in the frequency domain by Fourier transforming the time domain results, respectively. It was found that the bandwidth of this antenna changes length and width of the patch, length and width of the slot, length of T-shaped feedline, position of the offset. Measured % bandwidth was 49.2 % in the center frequency 2.5 GHz. These results were in relatively food accordance with calculated values.