• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.1
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• pp.103-114
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• 2006

Herbal mixture water extract of (Chrysanthemum morifolium, Portulaca oleracea, Sanguisorba officinalis, Sophora flavescens, Phellodendron amurense, Cnidium monnieri) which exhibit several beneficial effects including acne and skin diseases, was tested for anti-microbial activity and anti-inflammation effects. The herbal mixture extract showed antimicrobial activity against Stapylococcus epidermis, and Propionibacterium acne. The growth of Stapylococcus epidermis, and Propionibacterium acne was inhibited completely by addition of 1.0% of the extract. Also in the present study we examined the mixture extract on compound 48/80 induced allergy and LPS induced cyclooxygenase-2(COX-2) gene expression in RAW 264.7 macrophage. The results indicated the ear swelling and histamine release induced by compound 48/80 were dose-dependently reduced, ranging 28-60%, and 48-72% , respectively. Furthermore the extract inhibited the expression of LPS-induced COX-2 proteins and mRNAs without an appreciable cytotoxic effects on RAW 264.7 cells. The LPS-induced cytokine gene expression including IL-$1{\beta}$, TNF-$\alpha$, and IL-6 were dose-dependently suppressed by the mixture extract. Based on these results, it is concluded that the herbal mixture water extract can be applied to the acne and skin diseases therapy.

• Korean Journal of Food Science and Technology
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• v.25 no.6
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• pp.683-688
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• 1993

The antioxidative effect of the 75% ethanol extracts of preliminary selected Fritillaria ussuriensis Max(Fs), Houttuynia cordata Thunb(Hc), Portulaca oleracea L.(Po) and Perilla frutescene Var javanica Hara cake(Pf) were tested on palm oil and lard by rancimat test. Each solvent fraction of chloroform, ethyl acetate(EtOAc), butanol and water, was also evaluated its antioxidative effect with some synergists, like as ascorbic acid, citric acid and ${\delta}-tocopherol$. Po extract showed higher antioxidative effect on lard and the fraction of all test plants were the most effective on palm oil and lard with ascorbic acid and ${\delta}-tocopherol$. When 200 ppm of EtOAc fraction of Fs, He and Po extract each with 200 ppm of ascorbic acid were added to palm oil, the antioxidative index(AI, induction time of oil containing each extract/induction time of test oil) were 1.60, 1.53 and 1.47 respectively and 200 ppm of EtOAc fraction of Po and Pf extract each with 200 ppm of tocopherol to lard, the AI were 3.19 and 3.63 respectively.

• YAKHAK HOEJI
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• v.43 no.2
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• pp.274-277
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• 1999

A study has been conducted to investigate the radioprotective effects of purslane extracts (P) when i.p. injected before irradiation (R). For studying the amino acid composition, three groups of ICR mice (7-week-old) were tested. The first group i.p. injected with purslane extract for 5 days were irradiated with 6 Gy of ${\gamma}$-radiation (P+R). The second group was irradiate with 6 Gy without any pretreatment (R). The third group was non-irradiated control (CT). Each group divided into two groups for the survival rate study, one injected with saline (S) and the other injected with purslane extract (P) for five days and then irradiated with 8 Gy. The amino acid composition of urine samples were analyzed with HPLC for 19 days after irradiation. A few kinds of amino acids such as lysine and methionine in urine from P+R group increased in comparison with those from CT or R group. The survival rate of P group maintained much higher than that of S group during experimental period. The results obtained may support that this plant has radioprotective substances by means of life-lengthening, not of urine amino acid components.

• Korean Journal of Organic Agriculture
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• v.21 no.1
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• pp.105-113
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• 2013

Antibacterial activity is an important feature for the development of antibiotics alternatives. Plant extract is considered as a promising alternative for organic farming. In this study, a total of 11 plants were extracted using ethanol to determine their antibacterial activities against to Staphylococcus aureus, Listeria monocytogens, Mannheimia haemolytica and Salmonella gallinarum. The synergistic interaction among plant extracts was also investigated. Plants used in this study were Carthamus nctoricus L. (pA), Poncirus trifollata Raf. (pB), Scutellaria balcalensis Georgi (pC) Prunus sargentii (pD), Cucurbita moschata $D_{UCH}$ Leaf (pE), Allium cepa L. peel (pF) Portulaca oleracea L. (pG), Xanthium strumarium L. (pH), Duchesnea chrysantha (pI), Cudrania tricuspidata (pJ) and Juniperus chinensis L. (pK). The pB and pA had the most broad antibacterial spectrum and the highest activity against to Staph. aureus among plant extract, respectively. In the synergistic interaction, the mixtures of pA and pC as well as pA and pF had batter antibacterial activity against to Staph. Aureus compared with other mixtures.

• Korean Journal of Agricultural Science
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• v.38 no.2
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• pp.191-198
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• 2011

Herbicidal effects and crop selectivity of aqueous leaf extracts of sorghum (Sorghum bicolor L.) were evaluated against several weed species for developing sustainable weed management in organic farming. Aqueous sorghum leaf extracts were highly phytotoxic to different weed species. No broadleaf weeds were germinated in the concentration of 5 fold or higher concentrated sorghum leaf extracts and 90% of seed germination was inhibited within that range in grass species. Sorghum leaf extracts strongly inhibited the growth of different weeds by pre-emergence and foliar applications in greenhouse condition. Foliar application of sorghum leaf extracts had a higher inhibitory effect than the pre-emergence application. Broadleaf weed species were more susceptible than grasses to the application of sorghum leaf extract in foliar applications than grasses. Galium spurium, Erigeron candensis, and Rumex japonicus were completely killed at the highest concentrated sorghum leaf extract both in pre-emergence and foliar application. Most broadleaf weed species were inhibited more than 80% at pre-emergence application at 50 fold concentrated sorghum leaf extract. G. spurium, E. candensis, R. japonicus, Eclipta alba, Plantago asiatica and Portulaca oleraeea were most susceptible to sorghum leaf extract in foliar application. Growth of most broad leaf weed species was suppressed by greater than 90% at 50 fold concentrated sorghum leaf extract. Most crop species were tolerant to sorghum leaf extract but shoot growth was slightly reduced by the application of 40~50 fold concentrated extracts, Sorghum leaf extract may used to control weeds in organic fanning without affecting the growth of crop.

• Korean Journal of Plant Resources
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• v.26 no.3
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• pp.374-382
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• 2013

The objective of this study was determined to evaluate ${\alpha}$-amylase, ${\alpha}$-glucosidase, pancreatic lipase inhibition in vitro and DPPH radical scavenging activity of the several Korean resources plants. The ${\alpha}$-amylase inhibitory activity of Salicornia herbacea, Erythronium japonicum (flower) and Phragmites communis (root) in water extract showed relatively high 62.8%, 66.5% and 69.3%, respectively. The ${\alpha}$-amylase inhibitory activity of Citrus junos (pericarp) and Cornus officinalis in methanol extract was found to have an effect with 32.8% in Citrus junos (pericarp) and 60.9% in Cornus officinalis. Corylopsis coreana in both water and methanol extract had the highest ${\alpha}$-glucosidase inhibitory activity of 81.7% and 89.5%, while the extract of Portulaca oleracea, Ficus carica and Citrus junos was not measured ${\alpha}$-glucosidase inhibitory activity at given experiment concentration. Depending on the extraction solvent and the plant species, it was observed that there was a significant difference in ${\alpha}$-glucosidase inhibitory activity. The pancreatic lipase inhibitory activity showed relatively higher in the methanol extract than water extract except pericarp of Citrus junos. The DPPH radical scavenging activity of selected plants was much difference between measured plant species, and showed that the increase was proportional to the concentration. These results suggested that selected plants had the potent biological activity on carbohydrate, lipid Inhibitory activity and antioxidant activity, therefore these plant resources could be a good materials to develop medicinal preparations, nutraceuticals or health functional foods for diabetes or obesity.

• Proceedings of the Korean Society of Community Living Science Conference
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• 2009.09a
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• pp.77-77
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• 2009

쇠비름(Portulaca oleracea L.)은 쇠비름과(Portulacaceae)에 속하는 일년생 초본으로 우리나라 각지에 5~9월에 걸쳐 자생하고 있다. 한국보다는 중국에서 식용으로 애용되고 있으며, 해열, 이뇨, 산혈 등의 효능이 있어 임질, 요도염, 유종, 대하증, 임파선염 등에 약용으로 사용하고 있다. 쇠비름의 성분으로는 vitamin $B_1$ 및 C, saponin, tannin, flavonoid, dopamine, noradrenalin 등이 보고되었다. 쇠비름에 관한 연구로는 항산화효과, 항균효과 및 해독효과가 발표되었으나, 고지혈증에 관한 연구는 미흡한 실정이다. 따라서 본 연구에서는 고콜레스테를 유발식이에서 쇠비름 에탄을 추출물이 혈청 중 지질에 미치는 영향에 대해 검토하고자 하였다. 실험동물은 6주령의 Sprague Dawley 흰쥐 수컷을 각 실험군당 10마리씩, AIN-93M을 기본으로 하여 대조군(고지방식이), 쇠비름 에탄올 추출물 투여군의 3군(고지방식이+25mg/kg, 75mg/kg, 125mg/kg)으로 나누어 4주간 실험 식이를 급여하고 사육기간 중 사료 섭취량, 체중증가율, 식이효율을 측정하였다. 실험 종료 후, 혈액을 채취하여 혈액에서의 total lipid, total cholesterol, triglyceride, HDL-cholesterol은 효소법을 이용한 kit(아산제약)을 사용하여 측정하였고, 심혈관계 질환의 위험도에 이용되는 동맥경화지수는(atherogenic index, AI) 계산식을 이용하여 산출하였다. 실험결과, 최종체중, 체중증가율, 식이효율 및 장기무게는 실험군 간의 차이를 나타내지 않았다. 그러나 고지방식이에 쇠비름 에탄올 추출물을 125mg/kg의 농도로 병합 투여했을 때 혈중의 total lipid, total cholesterol 및 triglyceride는 대조군에 비해 유의적으로 감소하였으며, 고지방식이에 의해 증가된 동맥경화지수도 대조군에 비하여 유의적으로 저하되었다. 그리고 고지방식이로 증가된 ALT 및 AST 활성이 쇠비름 에탄올 추출물 투여로 유의적으로 감소하였다. 따라서 쇠비름 에탄올 추출물은 혈청 중 지질 개선 효과와 간 기능 개선효과가 있는 것으로 사료되나, 어떤 성분이 콜레스테롤 저하 효과를 나타내는지 보다 자세한 연구가 요구되어 진다.

• Journal of Nutrition and Health
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• v.37 no.8
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• pp.655-661
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• 2004

This study was performed to investigate the antimicrobial effect of the Pulsatilla koreana extracts against food-borne pathogens. First, the Pulsatilla koreana was extracted with methanol at room temperatures, and fractionation of the methanol extracts from Pulsatilla koreana was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol respectively. The antimicrobial activity of the Pulsatilla koreana extracts was determined using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of Pulsatilla koreana showed the highest antimicrobial activity against Staphylococcus aureus, Salmonella enteritidis and Shigella dysenteriae. The Staphylococcus aureus and Shigella dysenteriae were inhibited by petroleum ether and chloroform extracts of Pulsatilla koreana as well as ethyl acetate extracts of Pulsatilla koreana. The synergistic effect has been found in combined extracts of Pulsatilla koreana and Portulaca oleracea as compared to each extracts alone. Finally, the growth inhibition curve was determined using ethyl acetate extracts of Pulsatilla koreana against Staphylococcus aureus and Shigella dysenteriae. The ethyl acetate extract of Pulsatilla koreana showed strong antimicrobial activity against Staphylococcus aureus at the concentration of 2,000 ppm. The 2,000 ppm of ethyl acetate extract from Pulsatilla koreana retarded the growth of S. aureus more than 12 hours and Shigella dysenteriae up to 9 hours.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.449-452
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• 2001

In this study. an efficacy study of Portulaca Extract (PE) and ${\beta}$ -glucan. candidates for cosmetic additives. was pedormed using artificial skin model (AS). The AS consists of collagen gel matrix populated by ATCC human skin fibroblast cell line that is overlaid with epidermal human skin keratinocyte cell line. Cytotoxicity and anti - inflammatory activity of PE and ${\beta}$ -glucan were assessed using monolayer and AS models by measuring cell viability and the secretion of interleukin -1 ${\alpha}$.

• Korean Journal of Medicinal Crop Science
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• v.19 no.2
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• pp.126-135
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• 2011

Various herbal medicinal extracts were examined for the development of cosmetic products with anti-oxidative and anti-wrinkle activity. First, total polyphenol contents and DPPH radical scavenging activities of herbal medicinal extracts were measured. Most herbal samples, except for DW extracts of Portulaca oleracea, Caesalpinia sappan, Taraxacum platycarpum, Carthamus tinctorius, and 70% EtOH extracts of Taraxacum platycarpum and Carthamus tinctorius, showed DPPH radical scavenging activity over 80% at a concentration of $400{\mu}g/m{\ell}$. SOD-like antioxidant activity of DW extracts of Syzygium aromaticum, DW extracts of Eriobtrya japonica and 70% EtOH extracts of Sophora japonica was measured as 40%, 35% and 80%, respectively at a dry matter concentration of $50{\mu}g/m{\ell}$. In elastase inhibition assay, DW extracts of Lycium chinense ($50{\mu}g/m{\ell}$) and 70% EtOH extracts of Areca catechu ($50{\mu}g/m{\ell}$) showed 50% and 40% of inhibition, respectively. At a concentration of $1.250{\mu}g/m{\ell}$, DW extracts of Lycium chinense and 70% EtOH extracts of Areca catechu showed 10% and 30% of collagenase inhibition, respectively. Skin and lotion samples were prepared using the two herbal extracts of high anti-wrinkle activity: Lycium chinense extract and Areca catechu extract. The storage stability of skin and lotion containing each of the selected herbal extracts was evaluated. pH and viscosity were used as stability indicators for the stability test under different storage temperatures and freeze-thaw cycle conditions. The skin and lotion containing each of DW extract of Lycium chinense and 70% EtOH extract of Areca catechu was showed high pH and viscosity stability. The skin and lotion containing DW extracts of Lycium chinense showed relatively higher stability than the skin and lotion containing 70% EtOH extract of Areca catechu, at cycle chamber and freeze-thaw conditions. In summary, these results indicated that cosmetics containing DW extract of Lycium chinense were relatively stable, and this herbal extract could be used as a stable functional cosmetic material.