• Animal cells and systems
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• v.8 no.4
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• pp.289-294
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• 2004

Bupleurum latissimum is a narrowly endemic and endangered plant, restricted to only two small populations on steep cliffs of a small island, Ulleung Island, in Korea. The genetic diversity and population differentiation in the two remnant populations of the species were investigated using RAPD (random amplified polymorphic DNA) analysis. The Neis gene diversities were 0.146 in the smaller population of 45 individuals, and 0.151 in the larger population of 61 individuals. The genetic variation was not significantly different between these two populations. Genetic diversity within populations was not low considering the very small size of populations. Analysis of molecular variance (AMOVA) revealed higher variation within populations (65.9%) than genetic differentiation between them (34.1%). B. latissimum revealed higher population differentiation than other outbreeding species. The differentiation of the populations corresponded to low gene flow (Nem = 0.482). The cluster and principal coordination analyses provide strong support for high population differentiation, showing that all individuals of the two populations have built up population-specific clusters. Although gene flow between the two populations of B. latissimum was limited, they have preserved relatively high levels of genetic variation.

• Animal cells and systems
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• v.2 no.2
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• pp.259-267
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• 1998

A deletion of one out of the two copies of 9-bp repeat sequence (CCCCCTCTA), between the cytochrome oxidase II and Iysine tranfer RNA (COII/$tRNA^{Lys}$) genes in human mitochondrial DNA (mtDNA) has been used as a polymorphic anthropological marker for people of east Asian origin, and to lesser extent, Pacific and African populations. We searched for the 9-bp deletion of the intergenic COII/$tRNA^{Lys}$ Lys region in two Korean populations (175 from Seoul and 38 from Cheju) and examine the distibution of this deletion in world populations. The 9-bp deletion was detected directly by electrophoresis of the polymerase chain reaction (PCR)-amplified nucleotide(nt) 8211-8310 mtDNA fragment. The frequencies of the 9-bp deletion were significantly different between the Seoul (16%) and Cheju (8%) populations. Examination of data from the world populations suggests a geographic gradient. The frequency reaches its highest values in some Pacific island populations and decreases along the southeast Asia-Siberia transect. In spite of this geographic gradient, Mongoloid populations including Korean, Chinese, Japanese, and Mongolian populations were relatively homo-geneous with regard to the 9-bp deletion type of the intergenic COII/$tRNA^{Lys}$ region. These results indicate Koreans are genetically related to northeast Asian populations, and have a maternal mongoloid ancestry. Therefore, the 9-bp deletion of the intergenic COII/$tRNA^{Lys}$ region will provide significant information to elucidate the historical patterns of migration of the Mongoloids.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.4
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• pp.421-427
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• 1995

To know the variation in the growth of the sea urchin, Pseudocentrotus depressus according to their localities, three different populations including the culture populations, the released populations, and the wild populations were investigated using the cluster analysis. For this purpose, pigment banding pattern on the genital plate was used as an age character. The population composed of the same year class was born between September 25th and October 5th every year. The population of 9 to 10 months old had no $r_1,\;whereas\;r_1$, was observed in the population of either 1 year and 10 or 11 months old, and both $r_1,\;and\;r_2$ in the population of either 2 year and 9 or 10 months old. Therefore, $r_1,\;and\;r_2$ radius were regarded as an annual ring. Growth in the culture populations was much better than that In either the released or the wild populations. However, when the cultured populations composed of 8 to 12 mm in test diameter was released to the shove near a culture farm, its growth appeared to be similar to that of the wild Populations. The wide distribution patterns of test diameter measured from the same year class of both the cultured and the wild populations were probably due to either long spawning period or growth differences between individuals. Except Yamaguchi and Cheju populations, the move closely located populations showed the more similar growth pattern. However, the growth of Cheju population appeared to be similar to that of Aba, Mogi, Toishi rather than Kasiwazima although Cheju Island was more closely located with Kasiwazima than other localities. In addition, the population of Yamaguchi, located at the highest latitute, revealed the similar growth pattern to that of Nomozaki, located at the lowest latitute.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.1
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• pp.31-37
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• 2019

Objective: At least eight local duck breeds have been recognized and documented as national germplasm of Indonesia so far. It is necessary to genetically characterize the local duck breeds for aiding conservation and future improvement strategies. Thus, this study was carried out to assess genetic diversity and phylogenetic relationship of eight local duck populations of Indonesia using microsatellite markers. Methods: In total, 240 individuals (30 individuals each population) from Alabio (AL), Bayang (BY), Magelang (MG), Mojosari (MJ), Pegagan (PG), Pitalah (PT), Rambon (RM), and Turi (TR) duck populations were genotyped using 22 microsatellite markers. Results: The results showed a moderate level of genetic diversity among populations, with a total of 153 alleles detected over all loci and populations, ranging from 3 to 22 alleles per locus. Observed (Ho) and expected heterozygosity (He), as well as polymorphism information content over all loci and populations were 0.440, 0.566, and 0.513, respectively. Heterozygote deficiency in the overall populations ($F_{IT}=0.237$), was partly due to the heterozygote deficiency within populations ($F_{IS}=0.114$) and moderate level of genetic differentiation among populations ($F_{ST}=0.137$). The most diverse population was MG (He = 0.545) and the least diverse population was AL (He = 0.368). The majority of populations were relatively in heterozygote deficiency (except AL), due to inbreeding. The genetic distances, phylogenetic trees, and principal coordinates analysis concluded that the populations can be grouped into two major clusters, resulting AL, MG, and MJ in one cluster separated from the remaining populations. Conclusion: The present study revealed a considerable genetic diversity of studied populations and thus, proper management strategies should be applied to preserve genetic diversity and prevent loss of alleles.

• Korean Journal of Medicinal Crop Science
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• v.13 no.5
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• pp.262-269
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• 2005

The objective of this study was to assess genetic diversity among 6 different wild ginseng populations from New York, Kentucky, North Carolina, Pennsylvania, Tennessee and Virginia, and to compare these wild populations to one cultivated population. RAPD markers were used to estimate the genetic difference among samples from the 7 populations. The 64 random primers were screened, and 15 primers were selected which exhibited the 124 highly reproducible polymorphic markers. The ratio of discordant bands to total bands scored was used to estimate the genetic distance within and among populations. Multidimensional scaling (MDS) of the relation matrix showed distinctive separation between wild and cultivated populations. The MDS result was confirmed using pooled chi-square tests for fragment homogeneity. This study suggests that RAPD markers can be used as population-specific markers for American ginseng.

• The Korean Journal of Ecology
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• v.18 no.2
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• pp.231-241
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• 1995

I examined the relationship between allocations to plant size components such as plant height, total leaf weight, and nuber of branches and to reproductive components such as the number and weight of male and female fowers in three populations of the monoecious, wind-pollinated Ambrosia artemisiifolia. In particular, the two types of gender of plants, phenotypic and functional gender, were related to resource allocation. Mean values of all plant size and reproductive components differed significantly among the three populations. Such inter-population differences did not exist for both photosynthetic and reproductive efforts, Plant size components were mostly positively correlated with reproductive components among plants. However, the relationships between photosynthetic effort and reproductive components were largely negative and inconsistent among populations. The phenotypic gender was much lover than 0.5 in all of the three populations. Pairwise correlations between functional gender and each allocation measurement such as plant size components, reproductive components, photosynthetic effort, and reproductive effort were not consistent across the three populations. These results suggest that the gender of A. artemisiifolia measured as flower production responds sensitively to environmental conditions which might differ whithin as well as among populations.

• Journal of Life Science
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• v.12 no.1
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• pp.9-13
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• 2002

Crepidiastrum lanceolatum ($H_{OUTT}$) $N_{AKAI}$ (Compositae) is distributed in East Asia including Korea. Genetic diversity and population structure of six C. lanceolatum populations in Korea and two populations in Japan were determined using genetic variation at 19 allozyme loci. The percent of polymorphic loci within the enzymes was 42.1%. Genetic diversity at the species level and at the population level was low (Hes : 0.077; Hep : 0.068, respectively), where-as the extent of the population divergence was relatively low ($G_{ST}$ : 0.093). One of the most striking features of this study was the more significant difference within populations than among populations. An indirect estimate of the number of migrants per generation (Nm : 2.44) indicated that gene flow was moderate among eight populations of the species. In addition, analysis of fixation indices revealed a slight heterozygosity deficiency in some populations and at some loci. Narrow geographic ranges, short-lived perennial herbaceous, and small population sizes are mainly associated with the low level of genetic variation.n.

• Journal of Aquaculture
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• v.16 no.2
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• pp.69-75
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• 2003

Genetic variability and population structure of 11 natural ayu, Plecoglossus altivelis populations and one hatchery stock were assessed by starch gel electrophoretic analysis with 10 enzyme coding loci. Three loci were polymorphic (lower than 0.95 in major allele frequency) in natural populations,2 loci in hatchery stock. The average number of alleles per locus was 1.38. Observed heterozygosities ranged from 0.0235 to 0.088 (0.055 on the average) in natural population while 0.0925 in hatchery stock. The genetic distance among natural populations measured 0.000047-0.005407 and no significant differentiation was observed among them. On the other hand, a signifcant genetic distance was found between natural populations and the hatchery stock with measuring 0.002032-0.O08605. The results in this study suggest that the hatchery stock has diverged from natural populations, and also that careful to maintain sustainable and effective population size (parents number) should be made.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.8
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• pp.1071-1078
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• 2006

Three buffalo populations viz. Bhadawari, Tarai and local buffaloes of Kerala were genotyped using 24 heterologous polymorphic microsatellite loci. A total of 140 alleles were observed with an average observed heterozygosity of 0.63. All the loci were neutral and 18 out of the 24 loci were in Hardy Weinberg Equilibrium. The $F_{IS}$ values (estimate of inbreeding) for 16 loci in all the three populations were negative. This indicated lack of population structure in the three populations. The effective number of immigrants was 5.88 per generation between the Tarai and Bhadawari populations which was quite high suggesting substantial gene flow. The genetic distances revealed closeness between the Tarai and Bhadawari populations which was expected from geographical contiguity. The FST values were not significantly different from zero showing no population differentiation. The Correspondence Analysis based on the allelic frequency data clustered the majority of the Tarai and Bhadawari individuals as an admixture.

• The Korean Journal of Zoology
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• v.30 no.4
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• pp.379-386
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• 1987

Karyotype of the ricessh Orygias latipes (Temminck et Schlegel) was investigated using 151 specimens of eleven populations in the southern Korea. Based on the karyotype analysis, two distinct chromosomal groups were found. One group, occurring in the Kum River, the Mankyong River, the Dongjin River, the Ysngsan River and the SEmjin River had a diploid chromosome number of 46 and arm number of 70, which contained one pair of "large" metacentric chromosomes. The other group, occurring in the Tamjin River, the Hyongsan River andthe all four island populations observed had a diploid chromosome number of 48 and arm number of 68. These results showed that 0. latipes of 6 inland populations was identical with the Chinese population in diploid number, arm number and presence of "large" metacentric chromosome, while the present species of island populations ana populations of the Tamjn R. and Hyongsan R. was similar to the Japanese population in chromosome number and arm number. The karyotype variation in populations indicated that karyotypes will probably provide useful information in studying zoogeography of this species.phy of this species.