• Microbiology and Biotechnology Letters
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• v.7 no.3
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• pp.149-155
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• 1979

Streptomyces sp. 115-5 was selected as the most active microorganism of about 200 strains for the production of chitinase. The enzyme was purified by (NH$_4$)$_2$SO$_4$ treatment, 1st-Sephadex G-100, DEAE-Cellulose, 2nd-Sephadex G-100 column chromatography, and evidence for homogenity was obtained from CM-Sephadex C-50 column chromatography and polyacylamide gel electrophoresis. The purified enzyme hydrolyzed chitin (N-acetyl glucosamine polymer) and chitosan (glucosamine polymer) but not cellulose. And with chitin as the substrate, a Km value of 3.6 mg of chitin per ml and a Vmax of 100 $\mu$mo1e fer hr were found. The activation of the chitinase was 3.66 kcal per mole. The molecular weight of the enzyme was esti-mated about 56,000 daltons by Sephadex G-100 chromatography and isoelectric point as pH 3.0.

• Analytical Science and Technology
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• v.19 no.3
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• pp.218-225
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• 2006

A molecularly imprinted polymer (MIP) was synthesized by radiation-induced polymerization (RIP), where the ferulic acid was used as a template molecule, 4-vinylpyridine as a monomer and ethylene glycoldimethacrylate (EGDMA) as a cross-linking monomer. The MIP was packed in a glass column using a slurry method for use in medium pressure liquid chromatography (MPLC). The MPLC column was tested for separation and purification of ferulic acid from the rice oil. When repeated three times, the MPLC separation/purification yielded the ferulic acid with the purity higher than ~99%. The chemiluminescence of the luminal (5-amino-2,3-dihydro-1,4-phtalazinedione) measured on a potato disc slide (5.0 mm thick) was enhanced in the presence of ferulic acid, while, without the ferulic acid, the chemiluminescence of luminol on the potato slice disc was not observed, which suggests the ferulic acid obtained from the rice oil can be useful for immunoassay.

• Advances in concrete construction
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• v.8 no.3
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• pp.165-172
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• 2019

Progressive collapse is one of the factors which if not predicted at the time of structure plan; its occurrence will lead to catastrophic damages. Through having a glance over important structures chronicles in the world, we will notice that the reason of their collapse is a minor damage in structure caused by an accident like a terrorist attack, smashing a vehicle, fire, gas explosion, construction flaws and its expanding. Progressive collapse includes expanding rudimentary rupture from one part to another which leads to total collapse of a structure or a major part it. This study examines the progressive collapse of a 5-story concrete building with three column eliminating scenarios, including the removal of the corner, side and middle columns with the ABAQUS software. Then the beams and the bottom of the concrete slab were reinforced by (reinforcement of carbon fiber reinforced polymer) FRP and then the structure was re-analyzed. The results of the analysis show that the reinforcement of carbon fiber reinforced polymer sheets is one of the effective ways to rehabilitate and reduce the progressive collapse in concrete structures.

• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.202-202
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• 2006

With a modern size exclusion chromatography (SEC) column, molecular weight analysis of a polymer sample takes about 10 min. However, it is desirable to reduce the analysis time further, in particular, for high throughput measurements required in combinatorial analyses or 2D-HPLC analyses. We implemented the high temperature SEC for the purpose. By inserting a narrow bore tubing between the column and the detector, a sufficient backpressure can be maintained to prevent the mobile phase from boiling and the effluent is cooled down enough when it reaches the detector. Therefore, a normal SEC detector can be used without any modification. The SEC resolution is greatly improved at the elevated temperature at high flow rate which allows high speed operation.

• Structural Engineering and Mechanics
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• v.72 no.6
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• pp.723-736
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• 2019

The efficacy of a technique for the rehabilitation and strengthening of RC beam-column connections damaged due to cyclic loading was investigated. The repair mainly uses epoxy resin infused under pressure into the damaged region to retrieved back the lost capacity and then strengthening using fiber reinforced polymer (FRP) sheets for capacity enhancement. Three common types of reduced scale RC exterior beam-column connections namely (a) beam-column connection with beam weak in flexure (BWF) (b) beam-column connections with beam weak in shear (BWS) and (c) beam-column connections with column weak in shear (CWS) subjected to reversed cyclic loading were considered for the experimental investigation. The rehabilitated and strengthened specimens were also subjected to similar cyclic displacement. Important parameters related to seismic capacity such as strength, stiffness degradation, energy dissipation, and ductility were evaluated. The rehabilitated connections exhibited equal or better performance and hence the adopted rehabilitation strategies could be considered as satisfactory. Confinement of damaged region using FRP sheet significantly enhanced the seismic capacity of the connections.

• Bulletin of the Korean Chemical Society
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• v.35 no.10
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• pp.3115-3118
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• 2014

• The Korean Journal of Nuclear Medicine
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• v.27 no.2
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• pp.270-276
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• 1993

$Technetium-^{99m}$ labeling method using bifunctional chelating agent cyclic DTPA has been evaluated with human polyclonal nonspecific IgG. IgG was conjugated with cyclic DTPA with various molar ratio. Reduction of $^{99m}Tc$ was done with $Na_2S_2O_4$ with various molar excess. Labeling efficiency and identification of polymer was confirmed with HPLC using TSK4000 SW column. Polymer was purified with 100 cm Sepharose 6LB column. Cultured $1{\times}10^9$ Staphylococcus aureus were injected into rat thigh 24 hours later labeled IgG was injected, and in vivo distribution was observed 4 and 24 hours thereafter. Reduction of $^{99m}Tc$ was optimal with the 10000-50000 times molar excess of $Na_2S_2O_4$. Polymer formation increased with increasing mloar excess of cyclic DTPA to IgG. Three step labeling-labeling DTPA conjugated IgG after reduction of $^{99m}Tc$-made more polymer than two two step labeling-simultaneous mixing DTPA conjugated IgG, $^{99m}Tc$ and $Na_2S_2O_4$. $^{99m}Tc$ blood clearance and lower uptake in the abscess and other organs. IgG conjugated with 200 times molar excess of cyclic DTPA showed slower blood clearance with 200 times molar excess of cyclic DTPA showed slower blood clearance than that of 200 times molar excess of cyclic DTPA showed slower blood clearance than that of 20 times molar excess. In the $^{99m}Tc$ labeling of IgG with cyclic DTPA for the immunoscintigraphy, obtimal labeling condition should be chosen, and effect of the $^{99m}Tc$ labeled IgG polymer should be considered.

• Journal of the Earthquake Engineering Society of Korea
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• v.23 no.1
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• pp.43-54
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• 2019

Existing reinforced concrete frame buildings designed for only gravity loads have been seismically vulnerable due to their inadequate column detailing. The seismic vulnerabilities can be mitigated by the application of a column retrofit technique, which combines high-strength near surface mounted bars with a fiber reinforced polymer wrapping system. This study presents the full-scale shaker testing of a non-ductile frame structure retrofitted using the combined retrofit system. The full-scale dynamic testing was performed to measure realistic dynamic responses and to investigate the effectiveness of the retrofit system through the comparison of the measured responses between as-built and retrofitted test frames. Experimental results demonstrated that the retrofit system reduced the dynamic responses without any significant damage on the columns because it improved flexural, shear and lap-splice resisting capacities. In addition, the retrofit system contributed to changing a damage mechanism from a soft-story mechanism (column-sidesway mechanism) to a mixed-damage mechanism, which was commonly found in reinforced concrete buildings with strong-column weak-beam system.

• Steel and Composite Structures
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• v.47 no.6
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• pp.781-794
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• 2023

In order to directly apply seawater and sea sand in construction without desalination, a type of square concrete-filled steel tube (CFST) encased with prefabricated seawater sea-sand concrete filled Polyvinyl Chloride (PVC)/Glass Fiber Reinforced Polymer (GFRP) tube column was proposed. Twenty short columns were tested under uniaxial loads, and the test parameters included inner tube types, seawater sea-sand concrete replacement ratios, concrete strength, the wrapping area of Carbon Fiber Reinforced Polymer (CFRP) strips and the thickness of GFRP tube. The effects of the parameters on failure modes, loading capacity, ductility and strain responses were discussed. All the tested specimens failed with serious buckling of the steel tubes and fracture of the inner tubes. The specimens had good residual bearing capacity corresponding to 64% to 88.9% of the peak capacity. The inner GFRP tubes and PVC tubes wrapped by CFRP strips provided stronger confinement to the core concrete, and were good choices for the proposed columns. Moreover, an analytical model for the composite column with different inner tube types was proposed.

• Computers and Concrete
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• v.18 no.5
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• pp.1053-1063
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• 2016

As concrete is most usable material in construction industry it's been required to improve its quality. Nowadays, nanotechnology offers the possibility of great advances in construction. For the first time, the nonlinear buckling of straight concrete columns armed with single-walled carbon nanotubes (SWCNTs) resting on foundation is investigated in the present study. The column is modelled with Euler-Bernoulli beam theory. The characteristics of the equivalent composite being determined using the Mori-Tanaka model. The foundation around the column is simulated with spring and shear layer. Employing nonlinear strains-displacements, energy methods and Hamilton's principal, the governing equations are derived. Differential quadrature method (DQM) is used in order to obtain the buckling load of structure. The influences of volume percent of SWCNTs, geometrical parameters, elastic foundation and boundary conditions on the buckling of column are investigated. Numerical results indicate that reinforcing the concrete column with SWCNTs, the structure becomes stiffer and the buckling load increases with respect to concrete column armed with steel.