• Journal of the Korean Society of Tobacco Science
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• v.20 no.1
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• pp.80-86
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• 1998

Changes in activity and classification of esterase isozymes during the tire cycle or Plodia inteipunctella (Hiibner) were investigated by the native polyacrylamide gel electrophoresis. The stage specificity in esterase activity and isozyme pattern was observed throughout the larvalpupal-adult transformation. The activity esterase was highest at the 3-day old adult stage, and the lowest level at the prepupal stage. A total of 12 esterase bands were identified throughout the development, and the bands showing high enzyme activity was observed in the middle part of gel. Twelve esterases on the basis of inhibition by the three types of inhibitors (organophosphates, eserine sulfate and sulfhydryl reagents) were classified into three class, namely, carboxylesterase (CE), arylesterase (ArE) and cholinesterase (ChE), and these classes contained 7, 3 and 2 isozymes, respectively.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2007.11a
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• pp.21-33
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• 2007

CPAM has been applied to the paper industry for the purpose of wet-end improvement for a long time. And molecular weight and charge density have been managed most important quality factors to make CPAM for this application. Recently branched CPAM was developed to improve retention and drainage characteristics and we considered branch degree of CPAM as important factor as molecular weight and charge density. In this experiment, we tried to investigate physical and chemical properties to determine branch degree and flocculation efficiency using Arbocell pulp which was recently developed micro size pulp and finally we applied retention and drainage test under the ONP stock condition.

• Journal of Environmental Health Sciences
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• v.24 no.2
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• pp.104-109
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• 1998

The objective of this study was to examine the effect of polymer coating on the initial microorganism attachment and the biofilm growth. Such as nonion(polyacrylamine), anion(CMC-Na) and cation polymer coagulant(chitosan and PEI) were used for coating material of the support carrier(acryl plate). When polymer coagulant was coated with 5, 10, 20, 35, 50, 100 and 200 mg/l on the surface of acryl plate, initial microorganism attachment increased and optimum concentration for the attachment was 35 mg/l. Biofilm growth experiments were conducted with the substrate loading of 12.7gSCOD/$m^2\cdot$ day using RBC. The polymer coagulants such as CMC-Na, polyacrylamide, PEI and chitosan coating on the acryl plate facilitated the biofilm growth of microorganisms. Until the biofilm dry weight grows up to 0. 0038g/cm$^2$, biofilm growth on the plate coated with cation polymer like chitosan was better than that on the coated plate of nonion(polyacrylamine), anion(CMC-Na) polymer coagulant.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.3
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• pp.169-173
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• 2000

On human chromoscomes, a short sequence of DNA is known to repeat a number of times. These are called variable number of tandem repeat (VNTR) or short tandem respeat (STR) which has a short core. VNTR and STR are used in the filed of forensic science, evolution, and anthropology. In this work, we examined allele frequencies of one VNTR (YNZ22) and three STRs (NeuR, D21S11, Humth01) in a korean population sample by polymerase chain reaction (RCP) followed by high-resolution polyacrylamide gel electro-phoresis (PAGE) with silver stain. Subsequently, the polymorphism information content (PIC) was calculated : the hifhest PIC was observed in the NeuR locus (0.95680) and lowest in the Humth01 locus (0.75809).

• BMB Reports
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• v.31 no.1
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• pp.106-110
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• 1998

We have employed a DNA-native-polyacrylamide gel electrophoresis (DNA-native-PAGE) assay system to characterize the enzyme activity of the endonuclease secreted by human B lymphoblastic IM9 cells. Experimental results clearly demonstrated that the endonuclease activity of IM9 cell culture medium is distinct from that of DNase I in the DNA-native-PAGE assay system. Immunoprecipitation analysis using anti-DNase I antibody showed that the secreted endonuclease is not recognized by the antibody. The secreted endonuclease was estimated using supercoiled plasmid DNA as a substrate. The pH optimum required for the catalytic activity was determined to be in the range of pH 6.6-7.4. No significant difference in the endonuclease secretion was observed by stimulation of the IM9 cells with interferon-${\gamma}$ or interleukin-$1{\beta}$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.6
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• pp.728-735
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• 1995

The peptidyl prolyl cis-trans isomerase(PPlase, EC 5.2.2.8) from Bacillus stearothermophilus SIC1 was extracted from the cells treated with by lysozyme. PPlase was purified from the cell extracts by heat treatment, ammonium sulfate precipitation, ion exchange chromatography and finally gel filtration (FPLC). The purity of purified the enzyme after Superose 12 column chromatography was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE). The molecular weight of the purified PPlase was estimated as 18,000 by SDS-PAGE. The 39 amino acid residues from the N-terminus were determined by the protein sequencer. The enzyme showed the optimum pH at 8.0 and was stable at the range of pH 7.0 to 8.0. The enzyme was considerably stable after heat treatment at $60^{\circ}C$ for 30 minutes, and the enzyme was quite stable up to $65^{\circ}C$. The presence of the PPlase in the refolding solution accelerated the isomerization rate of the assay peptide.

• Biomedical Science Letters
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• v.8 no.4
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• pp.245-250
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• 2002

Fibrinolytic enzyme has been purified from the edible mushroom, Tricholoma sejunctum using DEAE-cellulose chromatography, Phenyl-Sepharose chromatography and Mono-S column chromatography. The apparent molecular mass of purified enzyme was estimated to be 17100 Da by SDS-polyacrylamide gel electrophoresis and 19000 Da by gel filtration, Indicating that it was a monomer. The N-terminal amino acid sequence of the enzyme was Ala-Thr-Tyr-Lys-Ile-X-Ser-Ala-Thr-His-Gln-X-X-Leu-Val. It has a pH optimum at pH 9.5, suggested that purified enzyme was a alkaline protease. The activity of purified enzyme was inhibited by EDTA and 1,10-phenanthroline, indicating that purified enzyme is a metalloprotease. The activity of purified enzyme was increased by Zn$^{2+}$ and Co$^{2+}$, however, the enzyme activity was totally inhibited by Hg$^{2+}$.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2017.05a
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• pp.51-63
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• 2017

The touch panel was developed decades ago and has become a popular input device in daily life. Because human-computer interaction is becoming more important, the next generation of touch panels require stretchability and bio-compatibility to allow integration with the human body. However, because most touch panels were developed based on stiff, brittle electrodes, electronic touch panels face difficulties to achieve such requirements. In this paper, for the first time, we demonstrate an ionic touch panel based on polyacrylamide hydrogel containing LiCl ions. The panel is soft and stretchable and thus, can sustain a large deformation. The panel can freely transmit light information through it because the hydrogel is transparent, with 99 % transmittance for visible light. A 1-dimensional touch strip was investigated to reveal the basic mechanism of sensing, and a 2-dimensional touch panel was developed to demonstrate its functionalities. The ionic touch panel was operated under high deformation with more than 1000% areal strain without sacrificing its functionalities. Furthermore, an epidermal touch panel on the skin was developed to demonstrate the mechanical and optical invisibility of the ionic touch panel through writing words, playing the piano and playing games.

• Preventive Nutrition and Food Science
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• v.7 no.2
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• pp.184-187
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• 2002

Blood products from slaughterhouses that are not hygienically prepared for disposal or food consumption pose a human health hazard. Gamma irradiation is an effective method for sterilization of blood products, but may introduce changes in the molecular characteristics of proteins. This study evaluated the effects of irradiation on animal plasma proteins. Bovine and porcine blood was obtained from a slaughterhouse and the plasma proteins purified and lyophilized. The secondary structure and molecular weight distribution of the plasma protein solutions and powders were examined after ${\gamma}$-irradiation at 1, 5, 7 and 10 kGy. Gamma-irradiation affected the molecular properties of the protein solutions, but not the protein powders. Circular dichroism and sodium dodecyl sulfate-polyacrylamide gel electrophoresis studies showed that increased doses of ${\gamma}$-irradiation decrease the ordered structure of plasma proteins in solution, and cause initial fragmentation of the polypeptide chains and subsequent aggregation.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.506-509
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• 2010

The application of Beauveria bassiana SFB-205 supernatant incorporated with polyoxyethylene-(3)-isotridecyl ether (TDE-3) significantly reduced the population of two species of aphids including cotton aphid, Aphis gossypii, and green peach aphid, Myzus persicae, much higher in cotton aphid, compared with supernatant incorporated with Tween 80, which allows the relationship of aphicidal activity with the degradation of aphid cuticles to be determined. Overall, the degradation of the cuticles induced by the supernatant was more remarkable in conjunction with TDE-3 than Tween 80, and this phenomenon was more observable in cotton aphid through SDS-polyacrylamide gel electrophoresis, revealing high correlation with their aphicidal activities.