• Journal of Plant Biology
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• v.33 no.1
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• pp.73-80
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• 1990

Subcellular distribution of arginase activity was measured in leaves of Canavalia lineata. Both mitochondrial and cytosolic fraction were found to contain the arginase activity. It was noticible that cytosolic fraction contained a substantial amount of arginase activity. Different mobility of arginase from these two fractions was showed on DEAE-Sephacel chromatography and polyacrylamide gel electrophoresis. Also different pI value was showed 6.3 in cytosolic and 6.7, 7.1 in mitochondiral fraction on IEF gel electrophoresis. However, canavaine-dependent-activity (CDA) of arginase in these two fractions were not different. These results indicate that heterogenity of arginase occurs in leaves of C. lineata.

• The Korean Journal of Zoology
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• v.15 no.2
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• pp.92-93
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• 1972

Ox-RBC로부터 Moddy法에 依하여 Ox-RCM抗原을 抽出하여 몇가지 分析을 實施하였다. Lyophilized 한 Ox-RCM protein의 化學的 分析 結果 total protein은 $55\\pm 1.3%$, total nitrogen은 9.2%, carbohydrate 8.5% 그리고 total acid soluble phosphorus는 $0.96\\pm 0.1%$였다. Polyacrylamide gel electrophoresis한 結果 Ox-RCM은 12 $\\sim$ 15個, Pre-VRS fraction은 8個 및 PHR 分劃은 9個의 pattern을 나타냈다. Amino acid의 分析 結果로 cystine이 4.93 $\\sim$ 3.72 moles/100 moles 이고 glycine이 最高로 26.7 $\\sim$ 13.5 moles/100 moles였고 最低로 methionine 이었으나 試料의 抽出方法에 따라 그 値의 變動을 볼 수 있다.

• The Korean Journal of Zoology
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• v.16 no.2
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• pp.146-148
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• 1973

Sal, typhi, Ty 2 菌體에 電離放射線을 照射하여 放射線 照射時 隋半되는 菌體의 化學的 性狀을 比較 分析하였다. 放射線 2.5 Mrad, 5.0 Mrad 照射로서 酸性 Amino acid는 增加하였으나 中性 및 鹽基性 Amino acid는 若干 減少하였다. 5.0 Mrad 照射로서 菌體의 總 Carbohydrate 量은 49.7%, 總 Nitrogen 量은 44.6%, Lipid 에 있어서는 Sterol 40%, Glyceride 30%, Phospholipid 14%의 減少를 各各 나타내었다. Polyacrylamide gel 電基泳動結果 放射線 照射群에서는 對照群에 比하여 Cell membrane protein의 變性을 認知할 수 있었다.

• KSBB Journal
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• v.19 no.5
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• pp.368-373
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• 2004

The optimal condition for the production of a glucan and a fructan synthesizing enzymes from Leuconostoc mesenteroides NRRL B-1149 were studied based on the different medium compositions. Response surface methodology was applied to find the optimistic condition showing the relationship between the fermentation response (enzyme activities) and the fermentation variable concentrations of yeast extract, peptone concentration, K2HP04 concentration and sucrose. Optimum medium composition for both enzymes production was $0.75\%$ yeast extract, $0.72\%$ peptone, $1\%$ K2HP04 and $2.17\%$ sucrose. Using this medium, the activities produced in culture was 0.90 U/m~ for glucosyltransferase (GTase) and 0.96 U/ml for fructosyltransferase (FTase). After purification of 1149FTase by consecutive chromatographies using Sephadex G-150 and DEAE-Sepharose, a 1149FTase of 210 kDa on $7\%$ polyacrylamide gel was isolated and it synthesized soluble fructan. The 1149GTase showed a band of 180 kDa on $8\%$ polyacrylamide gel after purification using Bio-Gel P-100 gel chromatography and DEAE-Sepharose ion exchange chromatography and it synthesized insoluble glucan. The linkages of polymers were determined by methylation using Hakomori reagent and following NMR analysis. The glucan was composed of a(1~6) and a(1~3) linkages and the fructan was levan.

• Microbiology and Biotechnology Letters
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• v.17 no.4
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• pp.385-391
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• 1989

D-Glucose isomerase (D-xylose ketol isomerase, EC 5.3.1.5) was purified from the Alkalophilic Bacillus sp. No. 1911 by ammonium sulfate precipitation, DEAE-cellulose ion exchange chromatography followed by Sephadex G-150 gel filtration chromatography. Molecular weight of the purified enzyme was estimated to be 11, 000 by gel filtration on Sephadex G-200, and SDS-polyacrylamide gel electrophoresis showed that the enzyme consisted of four identical or similar subunits with a molecular weight of 43, 000. The enzyme was the most active at pH 7.5 and $65^{\circ}C$, and stable up to 7$0^{\circ}C$ at pH 7.5 and in the range of pH 6-9 at 6$0^{\circ}C$ by 30 min incubation in the presence of Co$^{++}$.

• Journal of the Korean Ceramic Society
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• v.41 no.9
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• pp.684-689
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• 2004

Hexagonal sheet typed $\alpha$-Al$_2$O$_3$ nano particles were synthesized successfully at various conditions with the aluminum sulfate solution extracted from kaolin through the conventional and microwave assisted polyacrylamide (PAA) gel methods. Shorter gel time and higher yield were obtained in the microwave assisted PAA gel method, compared to conventional method. the size of nano particles was not changed by increasing the concentration of aluminum sulfate, but decreased with increasing the concentration of cross-linking agent in both the conventional and microwave samples. Above 0.01M concentration of cross-linking agent, the sizes of nano particles in the microwave samples were relatively larger than those in the conventional samples.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.259-263
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• 1998

High molecular weight glutenin (HMW-Glu) subunit compositions of 73 Korean wheat cultivars and experimental lines were evaluated by using one dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This method is suitable for obtaining a good resolution of 1Dx2 and 1Ax2$^*$ without adverse effects on separation of other HMW-Glu subunits. Korean wheats examined in this study could be divided into 15 different groups on the basis of HMW-Glu subunit compositions. From the wheat lines tested, it was identified that there were three alleles at the Glu-Al, five at the Glu-Bl and three at the Glu-D1 loci. The null allele of the Glu-Al was occurred in high frequency (79.4%), while low frequencies for 1Ax1 (12.3%) and 1Ax2$^*$(8.2%) were found. High frequency (75.3%) of the subunit pairs of 1Bx7+1By8 at the Glu-Bl loci compared with other subunits was found. The frequencies of subunits 1Dx2. 2+1Dy12 and 1Dx2+1Dy12 from the Glu-D1 loci were 54. 8% and 37.0%, respectively. However, a few Korean wheat lines (8.2%) carried 1Dx5 + 1Dy10 subunit pair which are responsible for good breadmaking quality. The information of HMW-Glu subunit compositions provide a useful tool to characterize wheat lines, and can be directly used in selection of breeding lines of different end-use properties.

• Transactions of the Korean Society of Mechanical Engineers
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• v.16 no.10
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• pp.1908-1914
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• 1992

A significant drag reduction in the turbulent flow of CWM(coal Water Mixture) adding minute amounts of high molecular weight polymer additives has been obtained and it was compared with pure CWM.However, the rate of drag reduction could come down with flow time, which is caused by polymer degradation, The rate of drag reduction and polymer degradation is affected by polymer type, concentration, molecular weight, and flow velocity. In the present investigation, these important parameters were evaluated for their influences on polymer degradation in order to find out stable conditions for CWM transportation with time. It was necessary to determine the more effective type of polymer additives to guarantee the optimum conditions for CWM transport. Experiments were undertaken with a test section of pipe diameter 9.8mm and pipe length 3500mm(L/D=357) in a closed loop, and polyacrylamide and polyetylene oxide were utilized as polymer additives. The tests were carried out under the conditions of 200, 400, 700ppm of polymer concentrations. CWM concentrations utilized were 5% and 10% with flow velocities of 4.9m/s and 6.1m/s. Experimental data show that polyehylene oxide degraded faster than polyacrylamide in CWM transport, and polyacryamide is considered to be a more effective candidate as additive for long time-CWM transport. Polymer degradation is also found to be more likely at lower polymer concentrations, at higher flow velocities, and higher CWM concentrations.

• Korean Journal of Food Science and Technology
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• v.20 no.1
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• pp.90-94
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• 1988

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunodiffusion method were used for the species differentiation of yeasts, Saccharomyces cerevisiae, Candida utils, Candida tropicalis, and Kleuyveromyces fragilis. Comparing the electrophoretic patterns of soluble and membrane proteins, Saccharomyces cereνisiae was similar to Candida utilis but was different from Candida tropicalis and Kleuyveromyces fragilis. In immunochemical properties of soluble proteins, Saccharomyces cerevisiae was almost identical with Candido utilis. However, Saccharomyces cerevisiae or Candida utilis was quite different from Candida tropicalis and Kleuyveromyces fragilis in their immunoreactivities. In immunochemical properties of membrane proteins, almost the same results were obtained irrespective of four yeast species. By using SDS-PAGE and immunodiffusion methods, Saccharomyces cerevisiae and Candida utilis were difficult to differentiate but both species were easily differentiated from Candida tropicalis and Kleuyveromyces fragilis.

• Journal of Sericultural and Entomological Science
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• v.27 no.1
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• pp.37-41
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• 1985

The haemolymph protein, digestive fluid proteins and digestive fluid amylase activity of wild silkworm, Theophila mandarina those of the were studied by polyacrylamide gel electrophoresis. In addition, they was also compared with silkworm. 1. 6 main protein bands in female and 7 main protein bands in male were detected in the larval haemolymph of T. mandarina where as 8 and 7 main protein bands in female and male of B. mori were observed. Some differences in the haemolymph protein ands of T. mandarina and B. mori were observed. 2. 15 protein bands and 12 protein bands were found in the larval digestive fluid of T. mandarina and B. mori respectively. Some differences in the mobility of digestive fluid proteins of T. mandarina and B. mori were noticed. 3. Larval digestive fluid amylases were anionic and moved near the tracking dye in both T. mandarina and B. mori. Mobility of the digestive fluid amylases relative to bromophenol blue were 0.019 and 0.020 in T. mandarina and B. mori respectively.