• Journal of Biomedical Engineering Research
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• v.15 no.3
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• pp.259-264
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• 1994

Heparin binders (Cell-PALA) used for selective heparin removal from blood, were prepared by immobilizing a cationic polymer, poly(allylamine) (PALA), onto cellulose substrate by a novel method. Their absorbing capacity for heparin was compared with untreated cellulose control using heparin solution in vitro. The surface areas of obtained heparin binders and untreated cellulose were 1.36 and ($2.56{\mu} g$/$cm^2$, respectively. The amount of bound heparin to PALA immobilized celluloses was determined to be 0.16 - $0.30{\mu}g$/cm, which is much higher than that of untreated cellulose ($0.03{\mu} g$/$cm^2$). These results suggest that Cell-PALA materials can be utilized for a heparin removal system.