• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.2
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• pp.99-105
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• 2005

We investigated oviposition and colony development of Bombus ignitus and B. terrestris depending on different pollen. In five kinds of pollen blend, the colony development in fresh-freezing pollen blend produced in Korea (Korea-FFP) was the best performance, although egg-laying characteristics is lower than that of pollen blend for oviposition imported from Korppert company (Kopport-FOP). The Kopport-FOP proved that it was suitable to use for oviposition of bumblebees. The Korea-FFDP, freezing dried-fresh pollen blend produced in Korea, is lower rather than the Korea-FDP although it is similar to the KopportFOP in colony development. It is not efficient to use commercial pollen for bumblebee because it is expensive in cost. The dried pollen blend for honeybee feeding imported from China (China-DP) was not suitable for rearing of bumblebee because it did not form colony although the worker emerged. In types of pollen, the oviposition and colony development of B. ignitus were not affected by the fresh-freezing pollen and dried-freezing pollen. This result also indicated that dried pollen, dried in the shade for 5 - 6 days, is possible to use as commercial pollen for bumblebee reproduction.

• Journal of Environmental Health Sciences
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• v.39 no.1
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• pp.43-47
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• 2013

Objectives: We investigated the effects of Korean gastrodia and bee pollen on testosterone production in Sprague-Dawley rats. Methods: This study measured the body weight, consumption of food and water, and serum testosterone concentration in SD rats fed for four weeks with gastrodia and bee pollen. Results: The average body weights after four weeks were $375{\pm}2g$ for the control group, $358{\pm}3g$ for gastrodia consumption, and $354{\pm}2g$ and $367{\pm}8g$ for acorn pollen and Siberian gooseberry pollen, respectively. The average food consumption in SD rats over the four weeks in the control was 23.6 g for gastrodia consumption, 24.3 g and 24.9 g for acorn pollen and Siberian gooseberry pollen, respectively. The average water consumption over the four weeks was 38.33 ml in the control group, 35.0 ml for gastrodia treatment, and 33.3 ml and 33.3 ml for acorn pollen and Siberian gooseberry pollen treatment, respectively. The testosterone secretion levels in the serum of male rats were increased by gastrodia, acorn pollen, and Siberian gooseberry pollen (2.68, 3.00, and 3.28 pg/ml, respectively) compared to the control group (2.23 pg/ml). Conclusions: These results suggest that gastrodia or bee pollen may be developed as a complementary medicine to improve sexual hormone production.

• The Korean Journal of Mycology
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• v.42 no.2
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• pp.165-169
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• 2014

In this study, we measured antioxidant activity as DPPH radical scavenging and the total polyphenol content of pulverized and lyophilized oak pollens inoculated with fungi to confirm the husk removal effect. The total polyphenol content of oak pollen was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in oak pollen germinated with A. mellea. The total polyphenol content of the lyophilized oak pollen germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH (2, 2 diphenyl-1-picrylhydrazyl) free radical scavenging method showed that the lyophilized oak pollen germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized oak pollen germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of acorn pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from oak pollen inoculated with A. mellea.

• Korean Journal of Medicinal Crop Science
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• v.21 no.5
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• pp.348-352
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• 2013

This study was carried out to investigate the effect of sucrose, boric acid, and calcium nitrate in optimum pH and relative humidity on pollen germination and pollen tube growth of boxthorn (Lycium chinense M.). Medium containing 100mg/L of $H_3BO_3$ showed maximum pollen germination and pollen tube elongation, 64.8% and $920{\mu}m$. Additionally supplied calcium nitrate to the medium lowered pollen germination rate and pollen tube elongation. Here we report the optimum conditions for the pollen germination and pollen tube development was observed at 10% sucrose, 1.0% agar, pH 6.0, and 100% relative humidity.

• Applied Microscopy
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• v.35 no.4
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• pp.74-83
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• 2005

The ontogeny of pollen wall in Panax ginseng was studied with transmission and scanning electron microscopy from early tetrad stage until pollen maturity. Initial indication of exine development is undulation of plasma membrane for the preparation of bacular mound. The first recognizable structure of the pollen wall is the cellulosic primexine which is formed outside of the plasma membrane while microspore tetrads are still surrounded by callose wall. As development proceeds, foot-layer and baculum differentiation, callose dissolution and exine formation were progressed. During this process, sporopollenin is deposited into the exine, and then endexine development was followed. The intine, innermost pollen wall layer, is developing form hypertrophic Golgi vesicles. The thickness of exine is very even on all along the pollen wall, but intine thickness of apertural region is thicker than that of nonapertural region. Mature pollen of ginseng is $20{\mu}m$ in size, tricolpate and shows fine reticulate sculpturing.

• The Korean Journal of Food And Nutrition
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• v.14 no.4
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• pp.339-345
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• 2001

Baking properties of Korean domestic wheat flour supplemented with pine pollen powder were investigated. Farinographic characteristics showed that the addition of pine pollen increased water absorption without any changes in dough development time and stability of dough in comparison with control. A higher gelatinization temperature and a lower maximum viscosity of dough on amylogram were observed with pine pollen powder addition. The addition of pine pollen powder showed decrease in redness and increases in lightness and yellowness of bread crumb. A significant increase in bread volume was observed as the pine pollen powder added more. Springiness. cohesiveness and resilience of bread were increased by pine pollen powder. Sensory evaluation of bread showed that the addition of pine pollen powder, especially at the level of 1%, enhanced color, mouth feeling, bleak and appearance without significant reduction of overall acceptability.

• Proceedings of the Botanical Society of Korea Conference
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• 1996.07a
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• pp.48-60
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• 1996

We previously identified and characterized a predominantly pollen-expressed gene of Petunia inflata that encodes a receptor-like kinase named PRK1. The extracellular domain of PRK1 contains leucine-rich repeats which have been implicated in protein-protein interactions, and the cytoplasmic domain was found to autophosphorylate on serine and tyrosine. To investigate the function PRK1 in pollen development, we transformed P. inflata plants with a construct containing the promoter of a predominantly pollen-expressed gene of tomato, LAT52, fused to an antisense PRK1 cDNA corresponding to part of the extracellular domain of PRK1, There transgenic plants were found to each produce approximately equal amounts of normal and aborted pollen. Analysis of the inheritance of the transgene inserts in two of the transgenic plants, ASRK-13 and ASRK-20, to their progeny revealed that certain transgene inserts cosegregated with the pollen abortion phenotype. Microscopic examination of the aborted pollen grains showed that their outer wall, the exine, was essentially normal, but that their cytoplasm contained only starch-like granules. Staining of the nuclei of the microspores at different stages of uninucleate stage. However, at subsequent stages half of the microspores completed mitosis and developed into normal binucleate pollen, but the other half initially remained uninucleate, then lost their nucleio. Analysis of the amounts of PRK1 mRNA and the antisense PRK1 transcript suggested that the pollen abortion phenotype most likely resulted from down-regulation of the PRK1 gene by the antisense PRK1 transgene. These results suggest that PRK1 plays an essential role in a signal transduction pathway that mediates post-meiotic development of microspores.

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.13-17
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• 1995

In order to induce haploid plant through pollen culture, pollens of Paeonia albiflora were cultured on MS liquid medium The development of micospore through pollen culture was examined The effect of low temperature (5$^{\circ}C$, 10 days) pretreatment on callus induction and embryogenesis in pollen culture was not evident Calli derived from pollen gave rise to globular embryos when transferred onto solid medium containing 0.5 mg/, 2,4-L. The effect of low temperature pretreatment and medium. combination to pollen viability was unrecognized. Pollen viability was reduced as the culture proceeded.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.4
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• pp.251-256
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• 2000

Astragalus membranaceus has flowers that are similar to that of the legume family, but shows poor bearing when self-pollination is induced. Thus, this study was carried out observing the ripening procedure of pistils and stamens and development stages of pollen in the context of the birth and growth of the flower. As to the bearing of the flower of A. membranaceus, few pod setting and 13% pod setting were observed when self-pollination is induced by paper-bag covering or artificial pollination treated respectively. The result indicates that A. membranaceus is a cross-pollination plant. A pistil grew faster than a stamen until just before blooming. The flower size was about 17.0mm$\times$4.0mm. Pistils and stamens had the same length after flowering. Pollen mother cells passed through meiosis and mitosis when its length reached around 3.5mm, thus creating the tetrade when 4 mm long. Pollen attained full growth when the bud was about 10mm long. An anther was found to tend to dehisce when the length of a bud reached around 12.0mm. As to the shape of pollen, about 70 % were normal. 1% and 30 % were small or empty pollen respectively. The result indicates that pollen of A. membranaceus attains full growth just before anther dehiscence which occurs before blooming while pistils grow faster than stamens until before flowering.

• Korean Journal of Agricultural Science
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• v.44 no.1
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• pp.23-29
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• 2017

This study was conducted to explore which pollen type could be an effective pollen source for self-incompatible 'Niitaka' pear (Pyrus pyrifolia Nakai), when different pollinizers were to be applied. The pollens of five different pears with similar flowering times were applied to bagged 'Niitaka' flowers grown in Cheonan city, Korea. Pollen types used were of different origins including Korean pears ('Wonhwang' and 'Chuhwangbae'), Japanese pears ('Chojuuro' and 'Okusankichi'), and Chinese pear ('Yali'). Results show that the amount of pollen produced by flowers of each cultivar was the largest in Korean pear 'Wonhwang' (234 g/100 flowers) and the smallest in Japanese pear 'Okusankichi' (126 g/100 flowers). A high proportion of large fruits (> 600 g), 71.4% of total production, was found in the fruits treated with 'Yali' pollen. However, these fruits showed an unfavorable external appearance. Based on individual fruit width differences, 'Chojuuro' treatment produced the smallest difference in fruit diameters (3.2 mm) and 'Yali' treatment' gave the largest (4.8 mm). Also, the difference in fruit length was the largest in 'Yali' (7.5 mm), which also produced the most uneven fruits among the pollinizers used. Meanwhile, the use of pollens of 'Wonhwang' and 'Chuhwangbae' improved productivity by increasing the fruit shape index and the ratio of bigger fruits in total production. Moreover, those pollens also decreased the development of lenticels on fruit skins which gave them a smoother appearance.