• The Plant Pathology Journal
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• 제34권6호
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• pp.532-543
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• 2018

Complete genome sequences of 22 isolates of Cucurbit aphid-borne yellows virus (CABYV), collected from melon plants showing yellowing symptom in Korea during the years 2013-2014, were determined and compared with previously reported CABYV genome sequences. The complete genomes were found to be 5,680-5,684 nucleotides in length and to encode six open reading frames (ORFs) that are separated into two regions by a non-coding internal region (IR) of 199 nucleotides. Their genomic organization is typical of the genus Polerovirus. Based on phylogenetic analyses of complete nucleotide (nt) sequences, CABYV isolates were divided into four groups: Asian, Mediterranean, Taiwanese, and R groups. The Korean CABYV isolates clustered with the Asian group with > 94% nt sequence identity. In contrast, the Korean CABYV isolates shared 87-89% sequence identities with the Mediterranean group, 88% with the Taiwanese group, 81-84% with the CABYV-R group, and 72% with another polerovirus, M.. Recombination analyses identified 24 recombination events (12 different recombination types) in the analyzed CABYV population. In the Korean CABYV isolates, four recombination types were detected from eight isolates. Two recombination types were detected in the IR and P3-P5 regions, respectively, which have been reported as hotspots for recombination of CABYV. This result suggests that recombination is an important evolutionary force in the genetic diversification of CABYV populations.

• The Plant Pathology Journal
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• 제32권6호
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• pp.580-583
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• 2016

The cotton leafroll dwarf virus (CLRDV) is one of the most devastating pathogens of cotton. This malady, known as cotton blue disease, is widespread in South America where it causes huge crop losses. Recently the disease has been reported from India. We noticed occurrence of cotton blue disease and chickpea stunt disease in adjoining cotton and chickpea fields and got interested in knowing if these two viral diseases have some association. By genetic studies, we have shown here that CLRDV is very close to chickpea stunt disease associated virus (CpSDaV). We were successful in transmitting the CLRDV from cotton to chickpea. Our studies indicate that CpSDaV and CLRDV in India are possibly two different strains of the same virus. These findings would be helpful in managing these serious diseases by altering the cropping patterns.

• 식물병연구
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• 제23권3호
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• pp.262-267
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• 2017

2015년부터 2016년까지 국내 5개 지역에서 기장 101점, 수수 200점의 시료를 채집하였다. 채집한 시료에 대해 paired-end RNA sequencing, RT-PCR 진단을 수행하였다. 그 결과, 경상도에서 채집한 수수에서 Rice black-streaked dwarf virus (RBSDV)가 검출되었으며, RBSDV, Rice stripe virus (RSV), Barley virus G (BVG), Cereal yellow dwarf virus (CYDV) 4종의 바이러스가 기장에서 검출되었다. 기장에서 검출된 4종의 바이러스 중 RSV와 RBSDV는 경상도에서 채집한 시료에서 높은 감염률을 보였다. 반면, BVG는 5개 지역에서 모두 감염이 확인되었으며, 이미 전국적으로 분포되어 있는 바이러스인 것으로 보인다. 본 연구에서는 국내 기장과 수수에서 RBSDV를 처음으로 동정하였으며, 수수에서 검출된 CYDV는 기존에 보고된 CYDV 분리주와 상동성이 상대적으로 낮은 것으로 보아 Polerovirus 속의 신종 바이러스로 예상된다.

• 식물병연구
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• 제23권1호
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• pp.69-74
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• 2017

2015년, 조 바이러스 발생양상을 구명하기 위하여 전국적인 조사를 실시하였다. 주요재배단지 7개 지역에서 이상 증상과 바이러스 병징을 보이는 식물체 100점을 수집하여, RT-PCR 진단과 RNA sequencing 방법을 이용하여 전체 4종의 바이러스를 동정하였다. 수집한 시료에서는 Barley virus G (BVG)가 10점, Rice stripe virus (RSV)는 4점, Northern cereal mosaic virus (NCMV), Sugarcane yellow leaf virus (ScYLV)가 각각 1점씩 검출되었다. 이들중에서 BVG와 NCMV는 국내 조에서 첫 발생보고이며, ScYLV는 Polerovirus속의 신종으로 예상된다. 본 연구결과는 조 식물체의 무병종자와 저항성 품종개발을 위한 기초연구 자료로 이용 가능할 것으로 생각된다.

• The Plant Pathology Journal
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• 제36권6호
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• pp.536-557
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• 2020

Sugarcane yellow leaf virus (SCYLV) is a distinct member of the Polerovirus genus of the Luteoviridae family. SCYLV is the major limitation to sugarcane production worldwide and presently occurring in most of the sugarcane growing countries. SCYLV having high genetic diversity within the species and presently ten genotypes are known to occur based on the complete genome sequence information. SCYLV is present in almost all the states of India where sugarcane is grown. Virion comprises of 180 coat protein units and are 24-29 nm in diameter. The genome of SCYLV is a monopartite and comprised of single-stranded (ss) positive-sense (+) linear RNA of about 6 kb in size. Virus genome consists of six open reading frames (ORFs) that are expressed by sub-genomic RNAs. The SCYLV is phloem-limited and transmitted by sugarcane aphid Melanaphis sacchari in a circulative and non-propagative manner. The other aphid species namely, Ceratovacuna lanigera, Rhopalosiphum rufiabdominalis, and R. maidis also been reported to transmit the virus. The virus is not transmitted mechanically, therefore, its transmission by M. sacchari has been studied in different countries. SCYLV has a limited natural host range and mainly infect sugarcane (Sachharum hybrid), grain sorghum (Sorghum bicolor), and Columbus grass (Sorghum almum). Recent insights in the protein-protein interactions of Polerovirus through protein interaction reporter (PIR) technology enable us to understand viral encoded proteins during virus replication, assembly, plant defence mechanism, short and long-distance travel of the virus. This review presents the recent understandings on virus biology, diagnosis, genetic diversity, virus-vector and host-virus interactions and conventional and next generation management approaches.