• Title/Summary/Keyword: pod

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Glucose Determination by Using Korean Radish Anionic Peroxidase (한국산 무 (Raphanus sativus L.) anionic peroxidase를 이용한 당 정량법 연구)

  • Kim, Jae-Hong;Kim, Sung-Ho;Lee, Mi-Young
    • Applied Biological Chemistry
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    • v.43 no.2
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    • pp.100-105
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    • 2000
  • Anionic peroxidases (POD) were isolated from Korean radish (Raphanus sativus L.) root by using fractionation with $(NH_4)_2SO_4$ and CM-cellulose ion exchange chromatography and used as the colorimetric enzyme for glucose determination. The chromogen used in this work was o-tolidine or 4-aminoantipyrine/diethylaniline (4AA/DEA) and the colored products were measured at 630 nm. Korean radish anionic POD showed much better colorimetric reaction of glucose determination with 4AA/DEA than with o-tolidine. The r values of calibration curve for glucose determination by o-tolidine and 4AA/DEA were 0.9983 and 0.9963, respectively. In order to compare the reactivity for substrate oxidation by Korean radish POD and horseradish POD, the Km values against o-dianisidine and guaiacol were measured. Korean radish POD had about 40 fold higher affinity for o-dianisidine and 2 fold higher affinity for guaiacol as revealed by Km values. These results showed that Korean radish POD could be developed as the colorimetric diagnosis reagent for glucose determination with high sensitivity.

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The Effect of the Turning Rate of the Pod Propeller on the Roll Control System of the Cruise Ship (크루즈선의 횡동요 제어시스템에 미치는 포드 각속도의 영향)

  • Lee, Sung-Kyun;Lee, Jae-Hoon;Rhee, Key-Pyo;Choi, Jin-Woo
    • Journal of the Society of Naval Architects of Korea
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    • v.49 no.1
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    • pp.14-25
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    • 2012
  • Recently, the application and installation of the pod propeller to the cruise ship is dramatically increased. It is because pod propulsion system allows a lot of flexibility in design of the internal arrangement of a ship. To reflect this trend, many researches have conducted to use the pod propeller for the roll stabilization of a ship. In the paper, a roll stabilization controller is designed by using fins and pod propellers as the control actuators for cruise ships. Two kinds of control algorithms are adopted for the roll control system; LQR (Linear Quadratic Regulator) algorithm and frequency-weighted LQR algorithm. Through the numerical simulation, the effect of the turning rate of the pod propeller on the roll control system is analyzed. Analysis of the simulation results indicated that the turning rate of the pod propellers is one of the important parameters which give the significant effects on the roll stabilization.

Growth and Optimum Harvesting Time of Pod-edible Peas (Pisum sativum L.)

  • Moon, Hyun-Sook;Hwang, Young-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.2
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    • pp.93-96
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    • 2000
  • The present study was performed to obtain the basic information about growth and quality related characteristics and optimum harvesting time for podedible pea which is a new crop in Korea but believed to have a great deal of potentials for both domestic and overseas markets. They can be consumed either as a fresh succulent vegetable or as tender green pods. The daily green pod yield of pod-edible peas started to increase from ten days after flowering and the maximum yield was recorded on 26 days after flowering. Ninety percent of pod yields could be harvested from 16 to 36 days after flowering. Mean green pod yield for the tested varieties was approximately 8.0 t/ha. Total vitamin C content of pod-edible peas showed continuously decreasing trends from five days after flowering. The highest sucrose content was obtained at ten days after flowering. The highest panel score based on sweetness, chewiness, and hardness for the processed green pods was shown at 10-15 days after flowering in all varieties tested, indicating that the optimum harvesting time for pod-edible peas was considered to be 10-15 days after flowering.

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Effects of Plant Types and Cultivars on Pod Yield in Late Seeding Peanut

  • Pae, Suk-Bok;Kim, Jung-Tae;Shim, Kang-Bo;Hwang, Chung-Dong;Chung, Chan-Sik;Lee, Myung-Hee;Park, Keum-Yong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.1
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    • pp.55-59
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    • 2007
  • This experiments were conducted to evaluate suitable plant-type and cultivars for producing fresh pod peanut from late seeding as succeeding crop, compared with early seeding as proceeding crop or single cropping. 12 cultivars according to grain weight and plant types, 6 virginia typed cultivars(ssp. hypogaea) and 6 shinpung typed cultivars(ssp. fastigiata), were used for early and late seedings. The plant growth and yield potential in early seeding were better than those in late seeding. But the ratios of dry/fresh pod and of mature pod in late seeding were higher than those of early seeding. The yield of fresh pod by cultivars in two seeding times showed significant correlation with pod scale such as fresh pod weight, 100-grain weight, and dry seed yield positively, but pod number negatively in early seeding only. Yield of fresh peanut between Virginia and Shinpung types didn't show significant difference in early seeding, but showed in late seeding. Average yield of Virginia typed cultivars showed significantly higher than that of Shinpung typed ones. This yield gap between two plant types was the same tendency on extending seedings to July 20.

An estimation method of full scale performance for pulling type podded propellers

  • Park, Hyoung-Gil;Choi, Jung-Kyu;Kim, Hyoung-Tae
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.6 no.4
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    • pp.965-980
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    • 2014
  • This paper presents a new estimation method of full scale propulsive performance for the pulling type podded propeller. In order to estimate the drag of pod housing, a drag velocity ratio, which includes the effects of podded propeller loading and Reynolds number, is presented and evaluated through the comparison of model test and numerical analysis. By separating the thrust of propeller blade and the drag of pod housing, extrapolation method of pod housing drag to full scale is deduced, and correction method of propeller blade thrust and torque to full scale is presented. This study utilized the drag coefficient ratio of the pod housing as a measure for expanding it to full scale, but in order to increase the accuracy of performance evaluation, additional study is necessary on the method for the full scale expansion via separating the drag of pod body, strut and fin which consist the pod housing.

Effects of Leaf and Pod Removal on Dry Matter Accumulation of Soybean Plants (적엽 및 제협처리가 콩의 건물축적에 미치는 영향)

  • 성락춘;박지희
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.4
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    • pp.324-329
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    • 1993
  • Effects of leaf and pod removal on changes in leaf and seed number, and leaf, seed, pod and stem dry weight of soybean [Glycine max (L,) Merr.] cultivar ‘Hwangkeumkong’ were measured at the research farm of Korea University in 1992. The upper 40% and lower 60% of leaves and pods were subjected to treatments at the growth stage of beginning pod(R3). Leaf number and dry weight of lower part were increased by upper leaf-lower pod removal, but seed number and dry weight were decreased. Upper leaf-lower pod removal increased lower stem dry weight and decreased upper pod dry weight. Leaf-seed ratio of the upper leaf-lower pod removal was the highest with 3.54 and harvest index was the lowest with 27% among the treatments. Lower leaf-upper pod removal showed that more assimilates from upper leaves were translocated to lower seeds than from lower leaves to upper seeds in upper leaf-lower pod removal treatment.

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Identification of SNPs tightly linked to the QTL for pod shattering in soybean[Glycine max (L.) Merr.]

  • Kim, Kyung-Ryun;Kim, Kyung Hye;Go, Hong Min;Lee, Ju Seok;Moon, Jung-Kyung;Ha, Bo-Keun;Jeong, Soon-Chun;Kim, Namshin;Kang, Sungtaeg
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.146-146
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    • 2017
  • The pod shattering or dehiscence is essential for the propagation of pod-bearing plant species in the wild, but it causes significant yield losses during harvest of domesticated crop plants. Identifying novel molecular makers, which are linked to seed-shattering genes, is needed to employ the molecular marker-assisted selection for efficiently developing shattering-resistant soybean varieties. In this study, a genetic linkage map was constructed using 115 recombinant inbred lines (RILs) developed from crosses between the pod shattering susceptible variety, Keunol, and resistant variety, Sinpaldal. A 180 K Axiom(R) SoyaSNPs data and pod shattering data from two environments in 2001 and 2015 were used to identify quantitative trait loci (QTL) for pod shattering. A major QTL was identified between two flanking single nucleotide polymorphism (SNP) markers, AX-90320801 and AX-90306327 on chromosome 16 with 1.3 cM interval, 857 kb of physical range. In sequence, genotype distribution analysis was conducted using extreme phenotype RILs. This could narrow down the QTL down to 153 kb on the physical map and was designated as qPDH1-KS with 6 annotated gene models. All exons within qPDH1-KS were sequenced and the 6 polymorphic SNPs affecting the amino acid sequence were identified. To develop universally available molecular markers, 38 Korean soybean cultivars were investigated by the association study using the 6 identified SNPs. Only two SNPswere strongly associated with the pod shattering. These two identified SNPs will help to identify the pod shattering responsible gene and to develop pod shattering-resistant soybean plants using marker-assisted selection.

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Change of SOD, POD Activity and Stomata Resistance for Ozone on Rice(Oryza sativa L.) (오존 처리에 의한 벼 품종간 SOD, POD 활성과 기공저항성의 변화)

  • Chung, Ill-Min;Kim, Kwang-Ho;Kang, Byeung-Hoa
    • Korean Journal of Environmental Agriculture
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    • v.19 no.2
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    • pp.160-165
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    • 2000
  • This study was carried to select the tolerance in rice varieites for ozone treatment through comparing SOD, POD and stomata resistance. In SOD Acivity, Sangnambatbyeo showed the highest activity on 1 hr after ozone treatment and after 24 hr, SOD activity was decreased. SOD activity of Hangangchalbyeo was not different in observed all times after ozone treatment. In POD activity, Hangangchalbyeo, Sangnambatbyeo and Ilpumbyeo were similar to POD activity but Baeknabyeo was the lowest activity on 1 hr after ozone treatment. Also, all varieties represnted the highest activity (above 90 %) an 24 hr after ozone treatment. In stomata resistance, Sangnambatbyeo was decreased stomata resistance on 1 hr after ozone treatment. Hangangchalbyeo, Sangnambatbyeo and Baekna were decreased stomata resistiance on 24 hr after ozone treatment except Ilpumbyeo.

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Effect of aerodynamic modifications on the surface pressure patterns of buildings using proper orthogonal decomposition

  • Tse, K.T.;Chen, Zeng-Shun;Lee, Dong-Eun;Kim, Bubryur
    • Wind and Structures
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    • v.32 no.3
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    • pp.227-238
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    • 2021
  • This study analyzed the pressure patterns and local pressure of tall buildings with corner modifications (recessed and chamfered corner) using wind tunnel tests and proper orthogonal decomposition (POD). POD can distinguish pressure patterns by POD mode and more dominant pressure patterns can be found according to the order of POD modes. Results show that both recessed and chamfered corners effectively reduced wind-induced responses. Additionally, unique effects were observed depending on the ratio of corner modification. Tall building models with recessed corners showed fluctuations in the approaching wind flow in the first POD mode and vortex shedding effects in the second POD mode. With large corner modification, energy distribution became small in the first POD mode, which shows that the effect of the first POD mode reduced. Among building models with chamfered corners, vortex shedding effects appeared in the first POD mode, except for the model with the highest ratio of corner modifications. The POD confirmed that both recessed and chamfered corners play a role in reducing vortex shedding effects, and the normalized power spectral density peak value of modes showing vortex shedding was smaller than that of the building model with a square section. Vortex shedding effects were observed on the front corner surfaces resulting from corner modification, as with the side surface. For buildings with recessed corners, the local pressure on corner surfaces was larger than that of side surfaces. Moreover, the average wind pressure was effectively reduced to 88.42% and 92.40% in RE1 on the windward surface and CH1 on the side surface, respectively.

Improvement of Peroxidase Productivity by Optimization of Medium Composition and Cell Inoculum Size in Suspension Cultures of Sweet Potato (Ipomoea batatas) (고구마(Ipomoea batatase)현탁배양에서 배지조성 및 세포접종량의 적정화에 의한 Pemxidase생산성 향상)

  • 곽상수;김수경;정경희;유순희;박일현;유장렬
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.2
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    • pp.91-97
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    • 1994
  • To improve the productivity of peroxidase (POD) of cell line SP-47 derived from cell suspension cultures of sweet potato (Ipomoea batatas (L) Lam.cv White Star), we optimized culture conditions including the composition and concentration of plant growth regulators and carbon source, and the cell inoculum size. When one g (fr wt) of cells was inoculated into 50 mL TL medium supplemented with l mg/L 2,4-D and 30g/L sucrose in 300 mL Erlenmeyer flask at 25$^{\circ}C$ in the dark (100rpm), the POD activity per g cell dry wt was maximized to be about 6,800 units after 25 days of subculture, which was about 30 times higher than that of intact roots of horseradish plants grown in the greenhouse, but the cell growth was maximum after 15 days of subculture. The protein content per g cell dry wt maintained almost plateau and after 25 days of subculture decreased as culture Proceeded further whereas the POD specific activity (unit/mg protein) was about two times higher after subculture and continuously increased from 12 days to the end of cultures (40 days). The POD isozyme patterns showed almost the same regardless of cell growth stage, but some acidic isozymes were slightly increased after 25 days of subculture. These results indicate that POD activity in suspension cultures of sweet potato is closely associated with cell growth and stresses derived from cell culture renditions and medium depletion. Due to its high POD activity the SPL47cell line seems to be suitable for the mass production of POD.

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