• 대한수의학회지
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• 제36권1호
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• pp.131-142
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• 1996

The effect of relative humidity in swine house on swin pleuropneumonia was examined in piglets experimentally infected with Actinobacillus pleuropneumoniae serotype 5. A total of 20 piglet were grown under 30~40%, 41~50%, 51~64% and 65~80% relative humidity chambers after intratracheal inoculation of A pleuropneumoniae. Characteristic fibrinous pleuropneumonia was observed in the pigs grown at the low relative humidity groups. The detailed results were as follows : 1. Growth performance and environment conditions were lower than high relative humidity groups. 2. Characteristic histopathological findings were fibrinous pleuritis and pneumonia accompanied congestion, hemorrhage, thrombosis and edematous change. 3. Antigenic distribution of inoculated bacterium was found mainly in alveolar macrophages or accumulated foci of macrophages adjacent to necrotic area. 4. Characteristic electron microscopic findings were proliferation of type II pneumocyte with increased lamella bodies and activated alveolar macrophages with pseudopods and widening of interstitium.

• 한국동물위생학회지
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• 제44권2호
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• pp.103-111
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• 2021

Actinobacillus (A.) pleuropneumoniae is the etiological agent of a porcine pleuropneumonia and have great economic importance to the global swine industry. For recent 5 years, a total of 50 pleuropneumonia cases of 24 pig farms were selected from pig lungs submitted to the College of Veterinary Medicine, Jeju National University using polymerase chain reaction (PCR) analysis. Collected lungs were fixed in 10% neutral phosphate-buffered formalin and processed for histological examination. Serotypes of A. pleuropneumoniae in pneumonic lesions were analyzed by PCR methods. And the antimicrobial susceptibility of A. pleuropneumoniae isolates was determined by a disc diffusion test. Grossly, unilateral distribution of hemorrhagic or necrotic pneumonic lesions was more common than bilateral distribution in lungs. In peracute or acute cases, histopathologic changes were characterized by necrosis, hemorrhage, neutrophils infiltration, vascular thrombosis, widespread edema and fibrinous exudates. Following the acute response, macrophage infiltration, marked fibrosis around zonal necrotic areas, and marked fibrous pleuritis were characteristic in chronic cases. A total of 50 pleuropneumonia were associated with A. pleuropneumoniae serotype 5 in 46 cases (92%), serotype 2 in 3 cases (6%), and both 2 and 5 in 1 case (2%). More than 90% of collected isolates showed high sensitivity to ceftiofur, amoxicillin, and colistin. However, ampicillin, penicillin, and tylosin showed low susceptibility. The results of this study demonstrated that A. pleuropneumoniae serotype 5 was predominant at porcine pleuropneumonia cases in Jeju.

• 한국임상수의학회지
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• 제32권5호
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• pp.454-458
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• 2015

1년령의 고양이가 호흡 곤란 주증으로 내원하여, 방사선, 초음파, CT 검사에서 우측 후엽의 폐 종괴, 다수의 흉벽 종괴를 동반한 흉막 비후, 다량의 흉수 소견이 확인되었다. 두 달 뒤 소장과 좌측 신장에서 다수의 결절들이 추가로 관찰되었고, 조직 검사와 면역 염색을 통해 고양이 전염성 복막염과 흉막 폐렴으로 진단되었다. 비삼출성 고양이 전염성 복막염에 의한 화농육아종성 폐렴이 발생한 한 마리 증례가 보고되어 있으며, 방사선 검사에서 폐엽의 경화상이 관찰되었다. 본 증례는 삼출성 고양이 전염성 복막염에서 흉막 폐렴이 다수의 종괴 양상으로 관찰된 드문 증례로 영상학적 특징을 평가한 첫 보고이다.

• 한국임상수의학회지
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• 제14권1호
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• pp.37-41
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• 1997

For the inspection of the occurrence situation of porcine pleuropneumonia and serotyping of Actinobacillus pleuropneumoniae strains isolated from lung lesions of pig in Korea, a series of experimentation have been carried out by the isolation and identification of A pleuropneumoniae, serotyping by coagglutination test, observation of lung lesion and clinical signs from 360 cases of porcine pneumonia in Clinical Pathology Laboratory, Bayer Veterinary Medical Research Institute. The results could be summarized as follows. The reaction of coagglutination between the reference antigens and the specific reagents of A pleuropneumoniae was strongly agglutinatied within 30 seconds without cross reaction. The 89 strains of A pleuropneumoniae were isolated from 360 cases of porcine pleuropneumonis and the biochemical properties of the isolates were same as the reference strains. The 89 isolated strains could be serotyped 39 strains as setotype 5, 34 strains as serotype 2, 8 strains as serotype 3, 2 strains as serotype 7 by coagglutination test, respectively. The clinical signs of pleuropneumonia were weakness, fever, anorexia, dyspnea and laboured breath in the later stages. The gross lesions of lung were haemorrhages, enlargement of interlobular septa, nodular formation and adhesion of the pleura.

• 대한수의학회지
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• 제24권1호
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• pp.99-104
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• 1984

Authors observed an outbreak of porcine pleuropneumonia at a piggery in Gyeongbug, Korea, which were diagnosed on the ground of clinical and pathological findings with the following results. 1. One hundred and forty weanlings out of 200 ones died suddenly without noticiable signs during the period of 15 days. All of the dead pigs showed formy, blood-tinged discharge from the nose and mouth. 2. Main clinical findings observed from 60 alive pigs from group consisted of fever, cyanosis severe degree of dyspnea and pleuritic friction rub. 3. On hematological observation for typical 5 cases leukocytes showed marked degenerative left shift with leukopenia (10500-13070/cmm), neutropenia (7-13%) and increase in the number of band neutrophils (6-27%). 4. The most prominent and constant pathological changes observed at necropsy for dead or alive pigs consisted of fibrinous pleurisy with partial adhesion, overlying of the pulmonary surface with fibrinous menbrane and necrotic pneumonic lesions with marked edema and congestion of lung parenchyma. 5. From these results the outbreak was considered to be typical porcine pleuropneumonia, although no bacteriological isolation was attempted.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.47.1-48
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• 2005

• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.1102-1105
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• 2001

In order to find therapeutic agents for porcine pneumonia, we screened far antibacterial activities of methanol extracts of 81 higher plants against four pathogenic microorganisms of Heamophilus parasuis, Pasteurella multocida, Actinobacillus pleuropneumonia, and Bordetella bronchiseptica, and found the bark of Cinnamomi cortex showed potent activities. Since this was inexpensive, we purified active compounds from it. The structures of the final active fractions were obtained through an activity-guided fractionation and their antibacterial activities are reported here.

• 한국임상수의학회지
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• 제1권1호
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• pp.53-61
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• 1984

Among the 308 breeding pigs imported via airway from Canada, 15 pigs died from the respiratory disease. Three of the carcases were examined by pathological and bacteriological means. In the lungs of the necropsied cases there was a lobar pleuropneumonia characterized by parenchymal hepatization, carnification and extensive adhesion between the thoracic organs due to fibrinous pleural thickening. Histologically the affected lungs had lesions of thrombosis and coalesced area of necrosis delineated by packed macrophages. A strain of Haemophilus Pleuropneumoniae was isolated from the affected lungs. The isolate was serologically identified as serotype 5.

• 한국동물위생학회지
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• 제23권3호
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• pp.255-262
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• 2000

A survey on pneumonic lungs and its histopathological changes of the slaughtered pigs from the southern area of Chungbuk province was carried out during the period from January to December 1999. Pneumonic lungs were attempted bactenological findings and antibiotic susceptibilities. The results obtained were as follows; 1. Of 158 slaughtered pigs, 97(61.4%) pigs had pneumonic lesions in the lung, and the prevalence was high in winter, spring, autumn, and summer in order. f. The bacteria isolated from pneumonic lesions were pasteurella spp, 13 heads(34.2%), streptococcus spp, 6(IS.8%), actinobacillus spp, 3(7.9%), coliform 4(10.5%) and the other bacteria, 12(31.6%). 3. These isolates were highly susceptible to the antibiotics of enrofloxacin 30(78.9%), cephalothin 23(73.6%) and ceftiofur 27(71 %). 4. Histopathologically, swine enzootic pneumonia and pleuropneumonia lesions were observed. The swine enzootic pneumonia lesions were consisted of peribronchiolar lymphoid hyperplasia and exudate in alveolar lumen. The pleuropneumonia lesions were consisted of thrombosis, alveolar wall thickened by mononuclear cells and neutrophil deposition.

• 한국동물위생학회지
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• 제23권3호
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• pp.289-299
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• 2000

The study was performed to investigate the distributions of swine pleuropneumonia in Kyongbuk western area by the enzyme-linked immunosorbent assay (ELISA). Sera collected from 400 slaughtered pigs in 3 slaughter houses during the period from May 1999 to october 1999 were tested to detect antibodies against A pleuropneumonie serotype 2 and 5. The optimal dilution of CBE antigen, conjugate and serum for this ELISA were determined 1 : 400, 1 : 20,000, 1 100, respectively. The optimal dilution of OW antigen, conjugate and serum for this ELISA were determined 1 : In, 1 : 20,000, 1 : 200, respectively. Cut-off value in this ELISA was determined by mean absorbance (at 492 nm) of negative control sera added with the triple value of the standard deviation. Cut-off value in ELISA by CBE and OMP antigen were 1.134 and 1.217, respectively. By the ELISA, positive reaction rates to A pleuropneumoniae serotype 2 and 5 for CBE antigen were 38.8% and 18.8%, and for OMP antigen were 42.8% and 23.5% of the 400 samples, respectively.