• Title/Summary/Keyword: plasma application

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The bactericidal effect of an atmospheric-pressure plasma jet on Porphyromonas gingivalis biofilms on sandblasted and acid-etched titanium discs

  • Lee, Ji-Yoon;Kim, Kyoung-Hwa;Park, Shin-Young;Yoon, Sung-Young;Kim, Gon-Ho;Lee, Yong-Moo;Rhyu, In-Chul;Seol, Yang-Jo
    • Journal of Periodontal and Implant Science
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    • v.49 no.5
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    • pp.319-329
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    • 2019
  • Purpose: Direct application of atmospheric-pressure plasma jets (APPJs) has been established as an effective method of microbial decontamination. This study aimed to investigate the bactericidal effect of direct application of an APPJ using helium gas (He-APPJ) on Porphyromonas gingivalis biofilms on sandblasted and acid-etched (SLA) titanium discs. Methods: On the SLA discs covered by P. gingivalis biofilms, an APPJ with helium (He) as a discharge gas was applied at 3 different time intervals (0, 3, and 5 minutes). To evaluate the effect of the plasma itself, the He gas-only group was used as the control group. The bactericidal effect of the He-APPJ was determined by the number of colony-forming units. Bacterial viability was observed by confocal laser scanning microscopy (CLSM), and bacterial morphology was examined by scanning electron microscopy (SEM). Results: As the plasma treatment time increased, the amount of P. gingivalis decreased, and the difference was statistically significant. In the SEM images, compared to the control group, the bacterial biofilm structure on SLA discs treated by the He-APPJ for more than 3 minutes was destroyed. In addition, the CLSM images showed consistent results. Even in sites distant from the area of direct He-APPJ exposure, decontamination effects were observed in both SEM and CLSM images. Conclusions: He-APPJ application was effective in removing P. gingivalis biofilm on SLA titanium discs in an in vitro experiment.

The Study of DNA Damage Induced by Atmospheric Pressure Plasma Jet and Their Mechanisms

  • Park, Yeunsoo;Song, Mi-Young;Yoon, Jung-Sik
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.155.1-155.1
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    • 2013
  • The goals of this study are to elucidate the plasma effects on DNA molecules to apply some plasma based applications and also to find out the mechanisms of plasma-induced DNA damage in biomolecule. Nonthermal atmospheric pressure plasma has much potential for medical, agricultural and food applications for the future. The atmospheric pressure plasma jet (APPJ) contains radicals, charged particles, low energy electrons, excited molecules and UV light. It has been started doing experiments using APPJ at the early 21th. And some recent results showed that APPJ has a possibility to apply to new fields like mentioned above. But it is kind of at the very early stages of plasma based application. It is definitely necessary much of theoretical and experimental studies to further understanding to use nonthermal atmospheric pressure plasma in biomedical, agriculture and food parts. Here we introduce a new experimental system to study plasma effects on biomolecules. And we will show some recent results of LEE-induced DNA damage using electron irradiation apparatus under ultra-high vacuum.

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Surface-Properties of Poly(Ethylene Terephthalate) Fabric by In-line Atmospheric Plasma Treatments (연속 대기압 플라즈마를 처리한 폴리에스테르 섬유의 표면 특성)

  • Kwon, Il-Jun;Park, Sung-Min;Koo, Kang;Song, Byung-Kab;Kim, Jong-Won
    • Textile Coloration and Finishing
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    • v.19 no.4
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    • pp.38-46
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    • 2007
  • Surface properties of the plasma treated fabric were changed while maintaining its bulk properties. Surface of plasma treated fabric take charge of enhanced adhesion by surface etching, surface activity. The water repellency coating Poly(Ethylene Terephthalate) fabric was treated with atmospheric pressure plasma using various parameters such as Argon gas, treatment time, processing power. Morphological changes by atmospheric pressure plasma treatment were observed using field emmission scanning electron microscopy(FE-SEM) and the zeta-potential measurement, contact angle measurement equipment. At the atmospheric pressure plasma treatment time of 150 sec, the power of 800W, the best wettability and peel strength were obtained. And we confirmed the possibility of industrial application by using atmospheric plasma system.

Lossless Single-Switch Inverter for Plasma Burner Drive Application (Plasma Burner 구동을 위한 무손실 단일 스위치 인버터)

  • Kang, Kyung-Soo;Kim, Sang-Yeon;Kim, Se-Min;Lee, Jeong-sun;Roh, Chung-Wook
    • Proceedings of the KIPE Conference
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    • 2015.07a
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    • pp.201-202
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    • 2015
  • 기존 Plasma Burner용 인버터 구동회로의 경우, Full Bridge Inverter를 사용하기 때문에 소자수가 많고 스위칭 손실이 큰 문제점이 있다. 본 논문은 Plasma Burner 구동을 위한 무손실 단일 스위치 인버터를 제안한다. 기존 Full Bridge Inverter를 사용하는 Plasma Burner 구동회로의 경우에는 4개의 스위치를 사용하기 때문에 스위치 손실이 매우 크고, Heat-sink 크기가 커지는 단점이 있다. 반면 제안된 Plasma Burner 구동을 위한 무손실 단일 스위치 인버터는 1개의 스위치를 사용하고, 스너버의 전력 손실이 최소화되기 때문에 전력 손실이 매우 작고 Heat-sink 최소화 및 고효율화가 가능한 장점을 갖는다. 본 논문에서는 제안된 Plasma Burner 구동을 위한 무손실 단일 스위치 인버터의 이론적인 특성을 분석하고 모의실험을 통해 확인하였으며, 15W급 Plasma Bunrer에 적용하여 실험을 통해 우수성을 검증하였다.

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Scanning Electron Microscopic Study of the Effects of Citric Acid on the Change of Implant Surface According to Application Time (구연산의 적용시간에 따른 임플란트 표면변화에 대한 주사전자현미경적 연구)

  • Song, Woo-Seok;Kwon, Young-Hyuk;Lee, Man-Sup;Park, Joon-Bong;Herr, Yeek
    • Journal of Periodontal and Implant Science
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    • v.32 no.4
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    • pp.697-709
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    • 2002
  • The present study was performed to evaluate the effect of citric acid on the change of implant surface microstructure according to application time. Implants with pure titanium machined surface, titanium plasma-sprayed surface, and sand-blasted, large grit, acid etched surface were utilized. Implant surface was rubbed with pH 1 citric acid for $\frac{1}{2}$ min., 1 min., 1 $\frac{1}{2}$ min., 2 min., and 3min, respeaively in the test group and implant surface was not treated in the control group. Then, the specimens were processed for scanning electron microscopic observation. The following results were obtained. 1. Both test and control group showed a few shallow grooves and ridges in pure titanium machined surface implants. There were not significant differences between two groups. 2. In titanium plasma-sprayed surfaces, round or amorphous particles were deposited irregularly. The irregularity of titanium plasma-sprayed surfaces conditioned with pH 1 citric acid was lessened and the cracks were increased relative to the application time of pH 1 citric acid. 3. Sand-blasted, large grit, acid etched surfaces showed the macro/micro double roughness. The application of pH 1 citric acid didn't change the characteristics of the sand-blasted, large grit, acid etched surfaces. In conclusion, the application of pH 1 citric acid to titanium plasma-sprayed surface is improper. And pure titanium machined surface implants and sand-blasted, large grit, acid etched surface implants can he treated with pH 1 citric acid for peri-implantitis treatment if the detoxification of these surfaces could be evaluated.

A Study on the Ralstonia Solanacearum Inactivation using Improved Plasma Process (개선된 플라즈마 공정을 이용한 Ralstonia Solanacearum 불활성화에 관한 연구)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.23 no.3
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    • pp.369-378
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    • 2014
  • Effect of improvement of the dielectric barrier discharge (DBD) plasma system on the inactivation performance of bacteria were investigated. The improvement of plasma reactor was performed by combination with the basic plasma reactor and UV process or combination with the basic plasma reactor and circulation system which was equipped with gas-liquid mixer. Experimental results showed that tailing effect was appeared after the exponential decrease in basic plasma reactor. There was no enhancement effect on the Ralstonia Solanacearum inactivation with combination of basic plasma process and UV process. The application of gas-liquid mixing device on the basic plasma reactor reduced inactivation time and led to complete sterilization. The effect existence of gas-liquid mixing device, voltage, air flow rate (1 ~ 5 L/min), water circulation rate (2.8 ~ 9.4 L/min) in gas-liquid mixing plasma, plasma voltage and UV power of gas-liquid mixing plasma+UV process were evaluated. The optimum air flow rate, water circulation rate, voltage of gas-liquid mixing system were 3 L/min, 3.5 L/min and 60 V, respectively. There was no enhancement effect on the Ralstonia Solanacearum inactivation with combination of gas-liquid mixing plasma and UV process.

Non-Thermal Atmospheric-Pressure Plasma Possible Application in Wound Healing

  • Haertel, Beate;von Woedtke, Thomas;Weltmann, Klaus-Dieter;Lindequist, Ulrike
    • Biomolecules & Therapeutics
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    • v.22 no.6
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    • pp.477-490
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    • 2014
  • Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.

A New Generation of Biocompatible Pulse-discharged Plasma by Marx Generator and Its Application on the Biomolecules

  • Park, Ji-Hun;Attri, Pankaj;Hong, Yeong-Jun;Kumar, Naresh;Kim, Sang-Yeop;Kim, Yeong-Jo;Lee, Gu-Hyeop;Lee, Seung-Mok;Park, Bong-Sang;Jeon, Su-Nam;Choe, Eun-Ha
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.240.2-240.2
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    • 2014
  • Characteristics of pulse-discharged plasma in liquid and its biological applications to proteins are investigated by making use of high voltage Marx generator. The Marx generator has been consisted of 5 stages, where each charging capacitor is $0.5{\mu}F$ to generate a high voltage pulse with rising time of $1{\mu}s$. We have applied an input voltage of 6 kV to the each capacitor of $0.5{\mu}F$. The high voltage pulsed plasma has been generated inside a polycarbonate tube by a single-shot operation, where the breakdown voltage is measured to be 7 kV, current of 1.2 kA, and pulse width of ${\sim}1{\mu}s$ between the two electrodes of anode-cathode made of stainless steel, which are immersed into the liquids. For the investigation of the influence of pulsed plasma on biomolcules, we have focused on the amino acids, DNA, proteins, cell and cholesterol.

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Effect of Sealing on the Corrosion Resistance of Plasma-Sprayed Alumina Coatings (실링이 플라즈마 스프레이 코팅된 알루미나 코팅재의 내부식성에 미치는 영향)

  • Kwon, Eui Pyo;Kim, Se Woong;Lee, Jong Kweon
    • Korean Journal of Materials Research
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    • v.32 no.10
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    • pp.442-447
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    • 2022
  • Sealing treatment is a post-surface treatment of the plasma spray coating process to improve the corrosion resistance of the coating material. In this study, the effect of the sealing on the corrosion resistance and adhesive strength of the plasma spray-coated alumina coatings was analyzed. For sealing, an epoxy resin was applied to the surface of the coated specimen using a brush. The coated specimen was subjected to a salt spray test for up to 48 hours and microstructural analysis revealed that corrosion in the coating layer/base material interface was suppressed due to the resin sealing. Measurement of the adhesive strength of the specimens subjected to the salt spray test indicated that the adhesive strength of the sealed specimens remained higher than that of the unsealed specimens. In conclusion, the resin sealing treatment for the plasma spray-coated alumina coatings is an effective method for suppressing corrosion in the coating layer/base material interface and maintaining high adhesive strength.