• The Korean Journal of Ecology
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• v.6 no.3
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• pp.227-235
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• 1983

This research was made over drought resistance and optimum soil moisture needed with Plantago asiatica L. as the material by means of making out the process of its growth under different soil moisture contents. The soil used for the experiment was a mixture of vermiculite and c-layer soil, and the process of growth was compared with each other controlling its soil mositure as: 7%, 15%, 30%, 45%, and 60%. In 7% range of soil moisture which was of low content, the increase of growth was neither significantly indicated nor any permanent seeding done. In view of this phenomenon, Plantago asiatica L. appeared to be highly drought-resistant. It was found rising at 30% range and reaching the optimum state at 45% range and falling down at 60% range range. In viw of this fluctuation indicated above, the optimum soil moisture content needed for the growth of Plantago asiatica L. is thought to be between 30% and 60%. It is thought the number of seed per capsule is not affected by the soil moisture content. It is expected an ecotypic variation by the soil moisture content will bring forth upon Plantago asiatica L.

• The Korean Journal of Food And Nutrition
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• v.30 no.3
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• pp.510-514
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• 2017

Plantago asiatica L., observed frequently in East Asia, is a known herb used in traditional medical remedies several studies report that P. asiatica L has anti-inflammatory and antioxidant effects. To determine the production of cytokines (IL-2, $IFN-{\gamma}$, and $TNF-{\alpha}$) induced by lipopolysaccharide (LPS) and non-LPS-stimulated macrophages, an ELISA assay was conducted using cytokine kits. Mice splenocytes were cultured for 48 h with various concentrations of P. asiatica L. (5, 10, 50, 100, 250, 500, and $1,000{\mu}g/mL$) or with mitogens (ConA or LPS). P. asiatica L. increased the proliferation of mice splenocytes, especially under the condition of its concentration ranging from 250 to $1,000{\mu}g/mL$. In addition, Plantago asiatica L. notably induced cytokine production of (IL-2, $IFN-{\gamma}$, and $TNF-{\alpha}$) at its concentration of $250{\sim}500{\mu}g/mL$. These results suggest that supplementation with P. asiatica L. water extracts may play a potential role in enhancing immune function by mediating splenocyte proliferation and cytokine production through its anti-inflammatory activit.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.26-31
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• 2016

Toxoplasmosis is an important cause of foodborne, inflammatory illnesses, as well as congenital abnormalities. Currently available therapies are ineffective for persistent chronic disease and congenital toxoplasmosis or have severe side effects which may result in life-threatening complications. There is an urgent need for safe and effective therapies to eliminate or treat this cosmopolitan infectious disease. The aim of this study was to investigate the in vitro anti-Toxoplasma activities of Plantago asiatica L., one of the herbal extracts, using tachyzoit of T. gondii RH strain infected HeLa cells. As the results, the selectivity of Plantago asiatica L. extract was 4.5, which was higher than Sulfadiazine selectivity (0.4). Also, we perfomed the cell proliferation inhibition test and the morphological study to evaluate the anti-T. gondii activity of Plantago asiatica L. extract with HeLa cells. As the results, the inhibition rate of the Plantago asiatica L. extract was high inhibition rate. This indicates that the Plantago asiatica L. extract may be used for new anti-T. gondii agent.

• Korean Journal of Pharmacognosy
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• v.27 no.2
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• pp.146-154
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• 1996

Korean folk medicine 'Jil-Kyung-Ee' has been used to cure female disease, acute gastritis, edema, abdominal pain and pleurisy. The botanical origin of the crude drug has not been confirmed pharmocognostically. To clarify the botanical origin of 'Jil-Kyung-Ee', the morphological and anatomical characteristics of the leaves and roots of Plantago species growing in Korea, i.e. P. asiatica L., P. camtschatica Cham., P. depressa Willd., P. lanceolata L., P. major L. var. japonica (Fr. et Sav.) Miyabe were compared. As a result, it was determined that 'Jil-Kyung-Ee' was the whole plant body of Plantago asiatica and Plantago camtschatica.

• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 1998.11a
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• pp.461-461
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• 1998

• KSBB Journal
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• v.29 no.3
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• pp.199-204
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• 2014

To investigate the effect of Plantago asiatica L. Root extract on skin care, we measured anti-oxidant activity and whitening action. As a result of measuring DPPH radical scavenging activity to examine independent anti-oxidation of PRE, there was slight scavenging activity. Fluorescent materials DHE, DCF-DA and DHR were each used to measure superoxide anion, hydrogen peroxide, and hydroperoxide created in RAW 264.7 cells, all concentrations were found to dependently prevent ROS production. Tyrosinase activation was found to be blocked dose-dependant. Melanin production was also prevented dose-dependant, but the effects were slight. Therefore, it is expected to be used effectively in development of functional cosmetic materials.

• The Korea Journal of Herbology
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• v.29 no.4
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• pp.77-82
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• 2014

Objectives : The effects of ethanol extract of Plantago asiatica L. were investgated on adipocyte differentiation, lipopogenesis, lipolysis and apoptosis using differnentiated 3T3-L1 adipocytes. Methods : Plantago asiatica L. was extracted with ethanol (CCE). We carried on MTT assay for cell proliferation, Oil Red O staining for determination of cell differentiation and intracelluar adipogenesis. TUNEL staining assay for cell apoptosis, and Western blot analysis for measurement of pAMPK and pACC, $C/EBP{\alpha}$, $PPAR{\gamma}$ protein expressions were performed. Results : The addition of CCE up to 0.2 mg/ml into cell culture media showed no cytotoxicity. Treatment of 0.2 mg/ml CCE significantly inhibited differentiation in 3T3-L1 preadipocytes. Lipid accumulation of the CCE treated cells was decreased compared with that of control. Induction of cell apoptosis was increased in CCE treated cells compared with that of control. AMPK and ACC levels of the cells with 0.2 mg/ml CCE were led to phosphorylation and also expressions of $C/EBP{\alpha}$ and $PPAR{\gamma}$, as adipogenic transcription factors, were suppressed compared with those of control. Conclusions : Taken together, these results provide evidence that CCE has a regulatory role in lipid metabolism that is related to differentiation into adipocytes, adipogenesis and apoptosis.

• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.439-440
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• 2010

• Korean Journal of Pharmacognosy
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• v.51 no.3
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• pp.222-229
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• 2020

Recently, consume for functional cosmetics containing natural products has been greatly increased. In order to develop it as a natural cosmetic material, we selected Ixeridium dentatum (Thunb.) Tzvelev, Plantago asiatica L. and Rumex crispus L. that have antioxidant and anti-inflammatory effects. In this study, simultaneous quantitative analysis of the isolated compounds and natural product complexes (Mix.) were validated using high performance liquid chromatography (HPLC). The isolated six compounds were shown in a large linearity with a correlation coefficient (R²) of 0.99. The limit of detection (LOD) of chlorogenic acid, plantamajoside, acteoside, emodin chrysophanol and physcion were 0.36 ㎍/mL, 0.36 ㎍/mL, 0.37 ㎍/mL, 0.30 ㎍/mL, 0.22 ㎍/mL and 0.12 ㎍/mL, respectively. The limit of quantification (LOQ) of chlorogenic acid, plantamajoside, acteoside, emodin chrysophanol and physcion were 1.10 ㎍/mL, 1.08 ㎍/mL, 1.12 ㎍/mL, 0.99 ㎍/mL, 0.66 ㎍/mL and 0.35 ㎍/mL, respectively. Content analysis showed chlorogenic acid (0.19 ± 0.02%), plantamajoside (0.48 ± 0.01%), acteoside (0.65 ± 0.01%), emodin (1.15 ± 0.11%), chrysophanol (0.73 ± 0.01%) and physcion (0.69 ± 0.09%). Therefore, the results of this study may provide for basic data of standardization research natural cosmetic material development on the I. dentatum, P. asiatica and R. cripus.

• Korean Journal of Pharmacognosy
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• v.38 no.4
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• pp.376-381
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• 2007

Seven compounds were isolated from the MeOH extract of the seeds of Plantago asiatica L. and their structures were identified as ${\beta}-sitosterol$ (1), (24R)-6${\beta}$-hydroxy-24-ethyl-cholest-4-en-3-one (2), acteoside (3), geniposidic acid (4), 1-octen-3-ol 3-O-${\beta}$-D-xylopyranosyl$(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (5), plantainoside D (6) and plantamajoside (7) on the spectroscopic analysis. Among them, $(24R)-6{\beta}$-hydroxy-24-ethyl-cholest-4-en-3-one (2) and 1-octen-3-ol 3-O-${\beta}$-D-xylopyranosyl ($1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (5) were first isolated from this plant. Among them, geniposidic acid (4) showed the most potent inhibitory effect on melanogenesis, with inhibition rate of 41%.