• 한국작물학회지
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• 제51권spc1호
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• pp.229-232
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• 2006

This study was conducted to select the most suitable dye bath soil of mat rush for good quality. Dry stem weight ranged from 102.5 to 107.0 g, dry stem ratio ranged from 34 to 36%, color of stem ranged from soft gray to heavy gray, degree of softness ranged from high to low and degree of floating ranged from extremely high to low. All the good quality components, such as dry stem weight, dry stem ratio, color of stem, degree of softness and degree of floating showed large variations. Hadong white ceramic soil, the superior dye bath soil of mat rush, showed relatively superior values for all the good quality components with 102.5 g in light dry stem weight, 34% in high dry stem ratio, good color of soft gray in color stem, high in degree of softness and extremely high id degree of floating. The results indicate that dye bath soils shows different adaptabilities to a particular dye bath and Hadong white ceramic soil seems to be the most suitable dye bath soil of mat rush for good quality.

• 한국응용곤충학회지
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• 제56권1호
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• pp.51-60
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• 2017

호박꽃과실파리(Bactrocera scutellata)는 호박꽃을 가해하는 해충이다. 이들 수컷은 식물체 유래물질인 raspberry ketone (RK)에 유인되어 섭식하는 습성을 지니고 있다. 본 연구는 이 해충의 방제를 위해 수컷박멸기술(male annihilation technique: MAT)을 개발하는 데 목표를 두고 이를 위한 기반 기술을 탐색하였다. 효과적 유인 물질이 실내 및 실외 실험을 통해 비교되었다. 일반적으로 과실파리류에 유인효과가 높은 methyl eugenol에 호박꽃과실파리 암수는 모두 반응하였으나 야외에서는 전혀 유인효과가 나타나지 않았다. RK의 합성 유도체인 cuelure(CL)에 호박꽃과실파리 수컷은 실내 및 야외조건에서 모두 높은 유인효과를 나타냈다. 그러나 본 연구에서 지향하는 왁스형 방출기 조건에서 RK가 CL에 비해 동등하거나 우수한 유인효과를 보였다. MAT를 구성하는 데 유인 물질과 함께 포함될 살충제를 피레스로이드 살충제로 선발하였다. 또한 MAT 처리 후 치사된 개체의 확인과 계수를 위해 깔때기형 트랩을 선발하였다. 이러한 결과를 종합하여 본 연구는 호박꽃과실파리의 MAT 기술은 RK과 비펜스린을 담은 왁스형 방출기로 결정하고 이를 깔때기트랩에 설치하도록 구성하였다. 이러한 기본 조건은 추후 현장 적용할 MAT 기술 개발에 기반 정보로 이용될 수 있다.

• Journal of Species Research
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• 제9권4호
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• pp.413-426
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• 2020

The Euphorbiaceae family features some of the most economically important plants that are sources of foods, oils, waxes, and medicines. The accurate identification of Euphorbiaceae species is critical in sustainable utilization of plant resources. We examined 234 sequences of nrDNA ITS, cpDNA rbcL and matK loci from 20 species in Euphorbiaceae in Korea and three outgroup taxa to develop efficient DNA barcodes. The three barcode loci were successfully amplified and sequenced for all Euphorbiaceae species. nrDNA ITS locus showed the highest mean interspecific K2P distance (0.3034), followed by cpDNA matK (0.0830), and rbcL (0.0352) locus. The degree of species resolution for individual barcode loci ranged from 75% (rbcL and matK) to 80% (ITS). The degree of species resolution was not enhanced with the different combinations of three barcode loci. The combined data set of the three loci(ITS+rbcL+matK) provided 80% of species resolution. These results confirm that ITS locus, as a single barcode, is the best option for barcoding of the Euphorbiaceae in Korea.

• 한국환경복원기술학회지
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• 제8권4호
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• pp.12-22
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• 2005

The purpose of this study is to select suitable planting base for the mat-type rooftop greening in order to popularize rooftop greening system easily. The experiment was conducted from 2004 June to 2005 May under several conditions; 4 soil depths under mats(2cm, 5cm, 10cm, 15cm), two soil mixtures(natural soil 80%+leaf mold 20%, artificial soil) and two light conditions(full sun place, 20% shaded place). In this experiment, 3 types of mats were used ; the herbaceous plants mat(11 plants inclusive of Lotus corniculatus L., Silene armeria L.), the lawn mat with Festica arundinacea and Sedum mat with Sedum kamtschaticum, Sedum sarmentosum, Sedum oryzifolium, Sedum middendorffianum. The result is as follows; in the mat-type rooftop greening, the herbaceous plants mat, lawn mat and sedum mat are the similar number of plant and effect of greening on soil depth 2cm, 5cm and 10cm, 15cm. So suitable soil depth of rooftop greening is 10cm for the load and economical factor. Thus the mat-type rooftop greening possible planting base depth of all 13cm as soil depth 10cm and mat depth 3cm. As soil mixtures, the number and growth of plants were better mat and 'natural soil 80% +leaf mold 20%' than mat and artificial soil. In herbaceous plants mat, Silene armeria L., Dianthus chinensis, Centaurea cyanus L., Lotus corniculatus L. are survival in full sun place and Silene armeria L., Dianthus chinensis, Centaurea cyanus L. are survival in 20% shaded place. In conclusion, selection of suitable soil mixtures and plants is possible extensive management rooftop greening with effect of continuous greening. The mat-type rooftop greening are lightweight and simple preparation without management and can popularize readily.

• 한국환경과학회지
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• 제31권3호
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• pp.247-254
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• 2022

This study investigated the effect of vegetation mat on plant growth and salt reduction in the soil treated with high concentration deicing salt. In order to measure soil chemical characteristics and plant growth, three native groundcover plants (Dendranthema zawadskii var. latilobum, Dendranthema boreale, and Kalimeris yomena) were grown in each of the three plastic containers (50.0 cm width × 35.0 cm length × 8.5 cm deep) with a high concentration treatment of calcium chloride deicing salt. There were two treatments: control, and BVM that combines B (blanket) and VM (vegetation mat). 1,600 g of soil was placed on the top of the drainage layer with 290 g of perlite, 100 seeds each of the three native plants with three repetitions were sowed, and 10 g/L of calcium chloride deicing salt was added in the treatment. As a result of the chemical properties of soil, soil in control treatment was acidic and soil electrical conductivity in BVM was the lowest. Also, exchangeable cations (K⁺, Ca²⁺, Na⁺, and Mg²⁺) in soil and all the three plants were significantly decreased in the BVM treatment. Meanwhile, the germination rate of Dendranthema zawadskii var. latilobum was the highest under high concentration deicing salt in compared to the two plants. Overall, three native groundcover plant growth was higher in the BVM than control treatment significantly. These results suggest that the treatment of blanket vegetation mat has a positive effect on soil and plant growth in soil damaged by deicing salt.

• 식물분류학회지
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• 제44권1호
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• pp.51-58
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• 2014

한국산 향나무절 식물에 대한 계통학적 유연관계를 규명하고, 더불어 향나무속 및 향나무절의 계통 및 유연관계를 잘 나타낼 수 있는 분자마커를 찾아내고자 분자계통학적 연구를 수행하였다. 엽록체 DNA matK와 psbA-trnH를 분자마커로 활용하였으며, 두 유전자의 조합분석결과 향나무절이 100%의 BP로 지지되는 분계조를 이루었다. 눈향나무+단천향나무 분계조와 향나무+섬향나무+뚝향나무 분계조는 각각 91%, 100%의 BP로 지지되었다. 따라서 한국산 향나무절은 (1) 눈향나무+단천향나무, (2) 향나무+섬향나무+뚝향나무 두 개의 분계조로 구분하는 것이 가장 적합할 것으로 생각되고, 본 연구에서 이용된 두 개의 분자마커 중 matK가 psbA-trnH 보다 향나무속 및 향나무절의 계통 및 유연관계를 규명하는데 다소 높은 해상력을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.502-509
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• 2005

Sexual reproduction plays an important role in the biology and epidemiology of oomycete plant pathogens such as the heterothallic species Phytophthora infestans. Recent worldwide dispersal of A2 mating type strains of P. infestans resulted in increased virulence, gene transfer, and genetic variation, creating new challenges for disease management. To develop a genetic assay for mating type identification in P. infestans, we used the Amplified Fragment Length Polymorphism (AFLP) technique. The primer combination E+AT/M+CTA detected a fragment specific to A1 mating type (Mat-A1) of P. infestans. This fragment was cloned and sequenced, and a pair of primers (INF-1, INF-2) were designed and used to differentiate P. infestans Mat-A1 from Mat-A2 strains. The Mat A1-specific fragment was detected using Southern blot analysis of PCR products amplified with primers INF-1 and INF-2 from genomic DNA of 14 P. infestans Mat-A1 strains, but not 13 P. infestans Mat-A2 strains or 8 other isolates representing several Phytophthora spp. Southern blot analysis of genomic DNAs of P. infestans isolates revealed a 1.6 kb restriction enzyme (EcoRI, BamHI, AvaI)-fragment only in Mat-A1 strains. The A1 mating type-specific primers amplified a unique band under stringent annealing temperatures of $63^{\circ}C-64^{\circ}C$, suggesting that this PCR assay could be developed into a useful method for mating type determination of P. infestans in field material.

• 식물조직배양학회지
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• 제25권6호
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• pp.487-487
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• 1998

• 식물분류학회지
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• 제45권3호
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• pp.243-253
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• 2015

붓꽃속(Genus Iris)의 금붓꽃계열(Series Chinensis)은 극동아시아에 국한되어 분포하고 있으며 한국에는 총 6분류군이 자생하고 있다. 이는 크게 두 개의 주요 그룹 (각시붓꽃 complex와 금붓꽃 complex)으로 나뉜다. 본 연구에서는 팔공산에서 발견된 잡종추정개체들의 실체와 붓꽃속 금붓꽃계열 분류군간의 계통학적 유연관계를 규명하기 위해 핵 rDNA ITS와 엽록체 matK 유전자의 염기서열을 확보하여 분석하였다. 총 55개체로부터 얻은 106 개 ITS amplicon의 염기서열 및 군외군의 염기서열을 분석한 결과, 금붓꽃계열의 노랑무늬붓꽃, 노랑붓꽃, 금붓꽃 및 잡종추정군은 군외군과 구분되어 유집되었으나, 군내군 사이에서는 높은 다형성 염기서열이 관측되었다. ITS 계통수에서 잡종추정군의 일부는 노랑무늬붓꽃과 하나의 분계조를 나타내었고, 나머지 잡종추정군의 경우는 금붓꽃+노랑붓꽃과 분계조를 형성하였다. 한편 cpDNA의 경우 matK를 제외한 나머지 마커는 금붓꽃과 노랑붓꽃의 차이를 보여주지 못하였다. matK의 NJ 계통수에서 잡종추정군이 금붓꽃과 높은 Bootstrap 값으로 하나의 분계조를 형성하였으며, 염기서열이 일치하였다. 이 결과를 바탕으로 팔공산에서 발견된 잡종추정군의 모계는 금붓꽃이고 부계는 노랑무늬붓꽃이라는 가능성을 제시하였다.

• Structural Engineering and Mechanics
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• 제54권1호
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• pp.169-188
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• 2015

This study presents the effects of the spatial variation of ground motions in a hard rock site on the seismic responses of a base-isolated nuclear power plant (BI-NPP). Three structural models were studied for the BI-NPP supported by different number of lead rubber bearing (LRB) base isolators with different base mat dimensions. The seismic responses of the BI-NPP were analyzed and investigated under the uniform and spatial varying excitation of El Centro ground motion. In addition, the rotational degrees of freedom (DOFs) of the base mat nodes were taken to consider the flexural behavior of the base mat on the seismic responses under both uniform and spatial varying excitation. Finally, the seismic response results for all the analysis cases of the BI-NPP were investigated in terms of the vibration periods and mode shapes, lateral displacements, and base shear forces. The analysis results indicate that: (1) considering the flexural behavior of the base mat has a negligible effect on the lateral displacements of base isolators regardless of the number of the isolators or the type of excitation used; (2) considering the spatial variation of ground motions has a substantial influence on the lateral displacements of base isolators and the NPP stick model; (3) the ground motion spatial variation effect is more prominent on lateral displacements than base shear forces, particularly with increasing numbers of base isolators and neglecting flexural behavior of the base mat.