• 제목/요약/키워드: plant-based protein

검색결과 487건 처리시간 0.026초

조선전통 식품으로 메주발효 (Meju Fermentation for a Raw Material of Korean Traditional Soy Products)

  • 이상선
    • 한국균학회지
    • /
    • 제23권2호통권73호
    • /
    • pp.161-175
    • /
    • 1995
  • 메주는 간장과 된장의 원료로 우리나라의 식물성 단백질인 조선 전통식품으로 중요하다. 조선 전통식으로 농가에서 만들어진 23종류의 메주덩어리를 수거하여, 29종의 균과 한 종의 세균을 채집하여 동정하였다. 이러한 중에서 다만 몇개의 미생물만이 메주발효과정에 작용하는 것을 발견하였고, 나머지 미생물은 메주발효에서 잘못 발효로 일어나는 오염 미생물로 밝혀졌다. 메주발효과정에서는 물리적인 변화로 건조과정(수분활성도)과 열방생과정이 메주발효 중에 일어나며, 이러한 과정이 미생물(대부분의 경우 메주 표면에서는 균)의 천이를 유도시켜 메주의 생화학적인 변화를 유도하는 것으로 나타났다. 균의 천이 과정에서 수분이 많았을 때는 물곰팡이인 접합균이 첨 서식한 것이 발견되었으며, 메주발효를 유도하는 것으로 나타났다. 흰포자를 방출하는 Scopulariopsis brevicaulis는 메주표면에서 고초세균인 Bacillus megatrium와 함께 후기 발열반응과정에서 발견되고 있었다. 미생물들이 분비하는 단백질 분해효소에 관한 조사에서, 각각의 미생물들은 콩 단백질 분해에 서로 상호보안적인 역할을 하였다. 또한, 접합균들은 조선 전통의 메주발효과정에서 후기 메주발효를 유도하는 중요한 균으로 관찰되었다.

  • PDF

여대생의 식습관과 영양소 섭취상태가 피부상태에 미치는 영향 (Effects of Dietary Habits and Nutrient Intakes on Skin Condition of Female University Students)

  • 조현주;최미자
    • Journal of Nutrition and Health
    • /
    • 제43권3호
    • /
    • pp.233-245
    • /
    • 2010
  • The purpose of this study was to investigate the effects of dietary habits and nutrient intakes on skin condition of female university students. The subjects were 95 female university students in Daegu. This study examined anthropometric measurements, dietary intake, food intake habits and skin condition (moisture, elasticity, sebum (U-zone, T-zone), pores, evenness and melanin pigmentation. The subjects in this study had an average age of 21.4, an average height and weight of 161.2 cm and 52.9 kg, respectively, and the average body mass index (BMI) was 20.3 $kg/m^2$. The means of skin elasticity, pores, evenness, and melanin pigmentation were within the normal range of women in their 20s and the skin moisture state was slightly lower than the normal range, which showed a dry tendency. The sebum in the U-zone and the T-zone was lower than the standard values, which showed less secretion in both areas. The subjects with high nut intake showed a better skin pore state (p < 0.05). Those with a high intake of instant foods showed a sig-nificantly low skin moisture and a significantly higher sebum rate (p < 0.05). The group with high skin elasticity and the group with less melanin pigmentation showed a significantly low animal protein intake (p < 0.05). The group with high skin elasticity also showed a significantly higher vitamin A and niacin intakes as well (p < 0.05). The group with high skin evenness showed a significantly lower animal lipid intake and a significantly higher vitamin E intake (p < 0.05). In addition, those with high skin elasticity and evenness as well as low melanin pigmentation showed a tendency of high intake of antioxidant vitamins, such as vitamin A, carotene, vitamin C or vitamin E. In summary, a moderate intake of plant-based products and also the antioxidant vitamins within such products seems to have a beneficial effect on maintaining a healthy and good skin condition.

Effects of Mixed Application of Chemical Fertilizer and Liquid Swine Manure on Agronomic Characteristics, Yield and Feed Value of Sorghum × Sudangrass Hybrid for Silage in Paddy Field Cultivation

  • Hwang, Joo Hwan;Lee, Sang Moo
    • 한국초지조사료학회지
    • /
    • 제34권3호
    • /
    • pp.155-162
    • /
    • 2014
  • This study was conducted to investigate the influence of the mixed application of chemical fertilizer (CF) and liquid swine manure (LSM) on the agronomic characteristics, dry matter yield, amino acids, minerals, and free sugars in cultivating Sorghum ${\times}$ Sudangrass hybrid (SSH) on paddy soil. The field experiment was designed in a randomized block design with three replications and consisted of CF 100% (C), CF 70% + LSM 30% (T1), CF 50% + LSM 50% (T2), CF 30% + LSM 70% (T3), and LSM 100% treatment (T4). The application of LSM was based solely on the nitrogen. Plant length, leaf length, leaf width and number of leaf were significantly higher in T4 (p<0.05), but stem diameter did not show significant differences among treatments. Stem hardness increased significantly (p<0.05) as the LSM application rate decreased, but sugar degree decreased significantly (p<0.05) as the LSM application rate decreased. Fresh yield, dry matter yield and TDN yield were the highest in T4, whereas the lowest in T2 (p<0.05). Crude protein, crude fat and crude ash were the highest in C, T4 and T2, respectively (p<0.05). However, NDF and ADF did not show significant difference among treatments. Crude fiber decreased significantly (p<0.05) as the LSM application rate increased. The total mineral content was decreased significantly (p<0.05) as the LSM application rate increased. Total amino acid content was higher in the order of T1> C> T3> T4> T2 (p<0.05). Free sugar content increased significantly (p<0.05) as the LSM application rate increased. The analysis of all the above results suggests that the application of liquid swine manure is very effective, considering the yield performance and the content of sugar degree and free sugar. In addition, liquid swine manure may be possible to grow Sorghum ${\times}$ Sudangrass hybrid without chemical fertilizer.

The Effects of CO2 Injection and Barrel Temperatures on the Physiochemical and Antioxidant Properties of Extruded Cereals

  • Thin, Thazin;Myat, Lin;Ryu, Gi-Hyung
    • Preventive Nutrition and Food Science
    • /
    • 제21권3호
    • /
    • pp.271-280
    • /
    • 2016
  • The effects of $CO_2$ injection and barrel temperatures on the physiochemical and antioxidant properties of extruded cereals (sorghum, barley, oats, and millet) were studied. Extrusion was carried out using a twin-screw extruder at different barrel temperatures (80, 110, and $140^{\circ}C$), $CO_2$ injection (0 and 500 mL/min), screw speed of 200 rpm, and moisture content of 25%. Extrusion significantly increased the total flavonoid content (TFC) of extruded oats, and ${\beta}$-glucan and protein digestibility (PD) of extruded barley and oats. In contrast, there were significant reductions in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, PD of extruded sorghum and millet, as well as resistant starch (RS) of extruded sorghum and barley, and total phenolic content (TPC) of all extrudates, except extruded millet. At a barrel temperature of $140^{\circ}C$, TPC in extruded barley was significantly increased, and there was also an increase in DPPH and PD in extruded millet with or without $CO_2$ injection. In contrast, at a barrel temperature of $140^{\circ}C$, the TPC of extruded sorghum decreased, TFC of extruded oats decreased, and at a barrel temperature of $110^{\circ}C$, PD of extruded sorghum without $CO_2$ decreased. Some physical properties [expansion ratio (ER), specific length, piece density, color, and water absorption index] of the extrudates were significantly affected by the increase in barrel temperature. The $CO_2$ injection significantly affected some physical properties (ER, specific length, piece density, water solubility index, and water absorption index), TPC, DPPH, ${\beta}$-glucan, and PD. In conclusion, extruded barley and millet had higher potential for making value added cereal-based foods than the other cereals.

Colletotrichum spp. 유래 scytalone dehydratase 유전자의 유전적 다양성 비교 (Genetic Variation of Scytalone Dehydratase Gene in Colletotrichum spp.)

  • 윤여홍;현민우;서동연;김용민;김성환;최창원;김영식
    • 한국균학회지
    • /
    • 제37권2호
    • /
    • pp.155-160
    • /
    • 2009
  • The production of dihydroxynaphthalene (DHN) melanin is known to be essential factor for pathogenicity in Colletotrichum lagenarium. However, the genetic diversity of melanin genes was not much known among Colletotrichum spp. To investigate the variability of melanin gene in Colletotrichum spp. that cause anthracnose on diverse crops including tomato, we cloned and sequenced partial sd, one of DHN melanin genes encoding for scytalone dehydratase, from eight strains of C. coccodes, C. acutatum, C. truncatum C. caricae, and C. musae. The size of PCR-amplified sd ranged 437 bp to 545 bp. The nucleotide sequence identity of sd among the Colletotrichum strains tested varied from 49% to 99%. All of the PCR-amplified sd from eight strains contain an intron and have two exons coding for 122 amino acids. Overall, the size and nucleotide sequence of sd varied among the five Colletotrichum spp. Sequence identity of the predicted scytalone dehydratase protein of 122 amino acids ranged 50 to 99%. Phylogentic analysis based on the sd nucleotide sequences revealed that the five Colletotrichum spp. could be genetically divided.

Purification and Characterization of a Serine Protease (CPM-2) with Fibrinolytic Activity from the Dung Beetles

  • Ahn, Mi-Young;Hahn, Bum-Soo;Ryu, Kang-Sun;Hwang, Jae-Sam;Kim, Yeong-Shik
    • Archives of Pharmacal Research
    • /
    • 제28권7호
    • /
    • pp.816-822
    • /
    • 2005
  • Catharsius protease-2 (CPM-2) was isolated from the body of dung beetles, Catharsius molossus, using a three step purification process (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and affinity chromatography on DEAE Affi-Gel blue). The purified CPM-2, having a molecular weight of 24 kDa, was assessed homogeneously by SDS-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of CPM-2 was composed of X Val Gin Asp Phe Val Glu Glu lie Leu. CPM-2 was inactivated by $Cu^{2+}\;and\;Zn^{2+}$ and strongly inhibited by typical serine proteinase inhibitors such as TLCK, soybean trypsin inhibitor, aprotinin, benzamidine, and ${\alpha}_1$-antitrypsin. However, EDTA, EGTA, cysteine, $\beta$-mercaptoethanol, E64, and elastatinal had little effect on enzyme activity. In addition, antiplasmin and antithrombin III were not sensitive to CPM-2. Based on the results of a fibrinolytic activity test, CPM-2 readily cleaved $A{\alpha}-$ and $B{\beta}$-chains of fibrinogen and fibrin, and y-chain of fibrinogen more slowly. The nonspecific action of the enzyme resulted in extensive hydrolysis, releasing a variety of fibrinopeptides of fibrinogen and fibrin. Polyclonal antibodies of CPM-2 were reactive to the native form of antigen. The ELISA was applied to detect quantities, in nanograms, of the antigen in CPM-2 protein.

Enhancing Seed Productivity and Feed Value of Oats (Avena sativa L.) with Different Seeding Rate and Nitrogen Fertilizing Levels in Gyeongbuk Area

  • Tomple, Byamungu Mayange;Hwan, Jo Ik
    • 농업생명과학연구
    • /
    • 제52권6호
    • /
    • pp.61-72
    • /
    • 2018
  • The aim of this present study was to investigate the impact of different seeding rate and nitrogen fertilizing levels on the seed yield and feed value of oats(Avena sativa L.) grown in Gyeongbuk area for two years. The experiment was arranged in split plot design with 3 main plots(100, 130 and 160 kg of seed/ha) and 4 subplots(0, 50, 70 and 90 kg N/ha), with 3 replicates. Heading, flowering, and maturing dates of oats sown in spring 2017, delayed by two days compared to that of spring 2016, and the plant height of spring 2017 were significantly shorter than that of spring 2016(18.1 ~ 23.4 cm). In addition, the highest number of stems and number of panicles according to different seeding rate and nitrogen fertilizing levels were achieved with the seeding rate of 160 kg/ha and 90 kg of N/ha in 2016 and 2017, respectively. In case of seed yield as affected by different seeding rate, the highest seed yield was achieved with a seeding rate of 130 kg/ha(p<0.05), and based on nitrogen fertilizing levels, the highest yield was obtained in 50 kg of N/ha compared to others. The crude fiber(CF), neutral detergent fiber(NDF) and total digestible nutrients(TDN) of oats cultivated and harvested in spring 2016 and 2017 according to different sowing rate were the highest in the seeding rate of 130 kg/ha. The crude protein(CP) content and total digestible nutrients(TDN) of 2016-2017 as influenced by nitrogen fertilizer levels were the highest in the nitrogen fertilizer level of 90 and 50 kg N/ha, respectively. In conclusion, the proper seeding rate and the optimal nitrogen fertilizing level in Gyeongju, Gyeongbuk province were considered to be the most appropriate in 130 kg/ha and 50 kg of N/ha, respectively.

Potential of four corn varieties at different harvest stages for silage production in Malaysia

  • Nazli, Muhamad Hazim;Halim, Ridzwan Abdul;Abdullah, Amin Mahir;Hussin, Ghazali;Samsudin, Anjas Asmara
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제32권2호
    • /
    • pp.224-232
    • /
    • 2019
  • Objective: Apart from various climatic differences, corn harvest stage and varieties are two major factors that can influence the yield and quality of corn silage in the tropics. A study was conducted to determine the optimum harvest stage of four corn varieties for silage production in Malaysia. Methods: Corn was harvested at four growth stages; silking, milk, dough, and dent stages from four varieties; Sweet Corn hybrid 926, Suwan, breeding test line (BTL) 1 and BTL 2. Using a split plot design, the treatments were then analysed based on the plant growth performance, yield, nutritive and feeding values followed by a financial feasibility study for potential commercialization. Results: Significant differences and interactions were detected across the parameters suggesting varying responses among the varieties towards the harvest stages. Sweet Corn was best harvested early in the dough stage due to high dry matter (DM) yield, digestible nutrient, and energy content with low fibre portion. Suwan was recommended to be harvested at the dent stage when it gave the highest DM yield with optimum digestible nutrient and energy content with low acid detergent fibre. BTL 1 and BTL 2 varieties can either be harvested at dough or dent stages as the crude protein, crude fibre, DM yield, DM content, digestible nutrient and energy were not significantly different at either stage. Further financial analysis showed that only Sweet Corn production was not financially feasible while Suwan had the best financial appraisal values among the grain varieties. Conclusion: In conclusion, only the grain varieties tested had the potential for silage making according to their optimum harvest stage but Suwan is highly recommended for commercialization as it was the most profitable.

Effects of Planting Density on Growth Characteristics, Dry Matter Yield and Feed Value of Teosinte New Variety, "Geukdong 6" [Zea mays L. subsp. mexicana (Schrad.) H. H. lltis]

  • Lee, Se Ho;Kim, Eun Joong;Lee, Sang Moo
    • 한국초지조사료학회지
    • /
    • 제42권3호
    • /
    • pp.162-168
    • /
    • 2022
  • This study was carried out to investigate the effects of planting densities on the growth characteristics, dry matter yield, and feed value of "Geukdong 6" (a new variety of corn for feed). The experimental design was arranged in a randomized block design with three replications. Treatments consisted of six planting densities, 60 cm × 25 cm (T1), 60 cm × 30 cm (T2), 70 cm × 25 cm (T3), 70 cm × 30 cm (T4), 80 cm × 25 cm (T5) and 80 cm × 30 cm (T6). All treatments were sown on May 14, 2021, and the harvest was on October 3 (late flowering). Plant length and the number of tillers were the highest in T5 (p<0.05), but the number of leaves and stem diameter were the highest in T6 than in the other treatments (p<0.05). Leaf length, leaf width, and dead leaf were not significantly different among the treatments. Organic matter was highest in T6, and crude protein was highest in T5 (p<0.05). The ether extract was not significantly different among the treatments. Crude fiber, NDF, and ADF were highest in T2 with relatively higher planting density (p<0.05). Calcium and phosphorus were not significantly different among the treatments. TDN content was the highest in T3 (p<0.05). Sugar degree (Brix), fructose, glucose, dextran, isomerose, and inverted sugar were not significantly different among the treatment. Fresh yield, dry matter yield and TDN yield were higher in order of T6 > T5 > T4 > T3 > T2 > T1 (p<0.05). Relatively feed value was higher in order of T3 > T6 > T5 > T1 > T4 > T2 (p<0.05). Based on the above results, planting density could be recommended from 80 cm × 30 cm for efficient production of "Geukdong 6".

Antioxidant effects of Cirsium japonicum var. maackii on oxidative stress in C6 glial cells and mice

  • Min Jeong Kim;Byeong Wook Noh;Qi Qi Pang;Sanghyun Lee;Ji-Hyun Kim;Eun Ju Cho
    • 농업과학연구
    • /
    • 제49권1호
    • /
    • pp.137-149
    • /
    • 2022
  • We investigated the effects of Cirsium japonicum var. maackii (CJM) against oxidative stress-induced C6 glial cells and cognitive impairment in mice. To evaluate the anti-oxidative effect of the extract and fractions from CJM, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), reactive oxygen species (ROS), and nitric oxide (NO) assays were conducted in H2O2-treated C6 glial cells. Furthermore, we identified the protective mechanisms of CJM with a scopolamine-treated mice model. The results revealed that H2O2 decreased the cell viability in C6 glial cells, indicating that H2O2 induced oxidative stress in glial cells. However, CJM fractions significantly increased cell viability in H2O2-treated C6 glial cells, which suggested that CJM protected against oxidative stress. CJM extract and fractions also reduced ROS and NO production, which were increased by H2O2 in C6 glial cells. In particular, the EtOAc fraction from CJM (EACJM) effectively protected against oxidative stress by increasing the cell viability and decreasing ROS and NO. Therefore, we carried out further in vivo experiments with EACJM. Scopolamine caused increases of ROS, thiobarbituric acid reactive substances (TBARS), and NO production. However, EACJM effectively alleviated ROS, TBARS, and NO levels compared to scopolamine-injected mice. In addition, EACJM up-regulated protein expressions of superoxide dismutase and glutathione peroxidase, indicating that EACJM enhanced the antioxidative system. Our results demonstrated that CJM had protective effects against oxidative stress in glial cells and memory dysfunction in mice. Based on these results, we propose that CJM could be a potential AD preventive and therapeutic agent.