• Korean Journal of Plant Resources
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• v.28 no.3
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• pp.363-369
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• 2015

Sweet potato has low regeneration capacity, which is a serious obstacle for the fruitful production of transgenic plants. Simple and rapid regeneration method from storage root explants of purple sweet potato (Ipomoea batatas L.) was investigated. The embryogenic callus was observed from 4 cultivars and its highest rate was induced at 1 μM 2,4-D after 5 weeks of culture. Result revealed that a low concentration of 2,4-D and low light intensity was important factors for embryogenic callus formation. After subculture on medium with 5 μM ABA for 4 days, subsequently, occurred the regeneration of shoots within 4 weeks when these embryogenic callus was transferred onto the MS hormone free medium. Regenerated shoots were developed into platelets, and grown normal plants in the greenhouse. We developed a simple and quickly protocol to regenerate plantlets in storage root explants of purple sweet potato. This regeneration system will facilitate tissue culture and gene transfer research of purple sweet potato.

• Korean Journal of Computational Design and Engineering
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• v.14 no.4
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• pp.225-233
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• 2009

The objective of this paper is to examine a construction method and verify PLC logic using the logical modeling and simulation of a virtual plant has complex manufacturing system and the domain of application is car body assembly line of automotive industrial operated by PLC Program. The proposed virtual plant model for the analysis of the construction method consists of three types of components which are virtual device, intermediary transfer and controller is modeled by logical model but it the case of the verification of PLC program, HMI and PLC logic in the field substitute for the controller. The implementation of the proposed virtual plant model is conducted PLC Studio which is an object-oriented modeling language based on logical model. As a result, proposed methods enable 3D graphics is designed in the analysis step to use for verification of PLC program without special efforts.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.562-566
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• 2010

SOD2-transgenic $T_3$ petunia line (A2-36-2-1-1-35) was treated with different levels of methyl viologen (MV) to determine its resistance to oxidative stress. Four (4) levels of MV (0, 100, 200, and $400\;{\mu}M$) were applied. The SOD2-transgenic $T_3$ petunia line exhibited a very significant oxidative stress resistance at the highest MV concentration ($400\;{\mu}M$) treatment compared to non-transgenic plant. RNA and protein expression of SOD2 transgene and higher parenchyma cell density in the transgenic petunias exhibiting resistance to oxidative stress proves its contribution to the expression of its resistance to oxidative stress.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.87.1-87
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• 2001

For a plant subject to several kinds of disturbances in the plant input side, we consider a problem of designing a controller based on the disturbance cancellation. The conventional loop transfer recovery (LTR) technique can not be used since the extended system consisting of the plant and the disturbance model is not necessarily stabilizable. We propose a new LTR technique that can be applied for our problem. As a target of the LTR, we choose a state feedback controller using a disturbance estimator. We find an LTR procedure based on the Riccati equation formalism where the stochastic model contains the filter gain matrix of the disturbance estimator in the target. The procedure recovers the target feedback ...

• Nuclear Engineering and Technology
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• v.54 no.10
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• pp.3595-3603
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• 2022

Nuclear safety-class DCS is used for nuclear reactor protection function, which is one of the key facilities to ensure nuclear power plant safety, the maintenance for DCS to keep system in a high reliability is significant. In this paper, Nuclear safety-class DCS system developed by the Nuclear Power Institute of China is investigated, the model of reliability estimation considering nuclear power plant emergency trip control process is carried out using Markov transfer process. According to the System-Subgroup-Module hierarchical iteration calculation, the evolution curve of failure probability is established, and the preventive maintenance optimization strategy is constructed combining reliability numerical calculation and periodic overhaul interval of nuclear power plant, which could provide a quantitative basis for the maintenance decision of DCS system.

• Journal of Plant Biology
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• v.32 no.4
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• pp.247-253
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• 1989

Protoplasts were enzymatically isolated from suspension culture of sweet potato. High yields of single protoplasts were produced from nonembryogenic cell aggregates. However, most protoplasts obtained from embryogenic cell clumps were spontaneously fused during enzyme treatment; a small portion of them remained single. Upon transfer to Murashige and Skoog's(MS) liquid medium supplemented with 0.1 mg/1 6-benzyladenine(BA) and 1 mg/12,4-dichlorophenoxyacetic acid(2,4-D), protoplasts from nonembryogenic cell aggregates sustained cell divisions to form cellus. Upon subculture onto MS media with 0.2 mg/12,4-D or without growth regulators, the callus did not give rise to any organs. On the other hand, first cell division of single protoplasts from embryogenic cell clumps was sporadically observed.

• The KSFM Journal of Fluid Machinery
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• v.19 no.3
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• pp.5-9
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• 2016

The purpose of this paper is to define criteria to be used as part of the engineering design for an oil sands plant equipped with the steam assisted gravity drainage process. In this effort, the oil treatment process of an oil sands plant on a pilot scale was focused for detailed investigation. The thermodynamic properties of the process fluid, which is mainly composed of bitumen and water, were estimated with the CPA model. The commercial tool aspen HYSYS was used for the analysis throughout this work along with the provided input data and some necessary assumptions. From the simulation results, the heat and mass balances for a 300 BPD plant were established in order to define standard data for its modular design. In particular, the basis of design for equipment size, heat transfer areas, capital cost and operation cost was extensively discussed.

• Journal of Korean Society of Water and Wastewater
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• v.24 no.2
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• pp.173-181
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• 2010

This study was conducted to investigate the possible installation of small hydro-power plant at the discharge point of Gumi sewage treatment plant (STP) using treated wastewater. Sufficient amount of water to transfer to electric power and the selection of proper location are two essential elements for the construction of small hydro-power facility. Preliminary analysis based on site visit and existing data in Gumi STP were made. Capacity of the small hydro-power plants and exact location were determined by geomorphological condition and flow duration characteristics. Flow duration characteristics and its duration curve were identified using monthly rainfall data in Gumi STP. Relevant facts of small hydro-power system in other STP were referred to adopt to Gumi STP situation. Flowrate of treated effluents and effective head between flow chamber and the location of hydraulic turbine in Gumi STP are found to be $3.70m^3$/sec and 3.5m respectively. Electric generation rate based on this feasibility study was estimated to be 86.3kW/h. Yearly electric generation rate was expected to be 932.4MMh. Proposed small hydro-power plant construction in Gumi STP is to be reasonable.

• Asian Journal of Atmospheric Environment
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• v.10 no.3
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• pp.162-167
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• 2016

This study was conducted in an industrial city (Bilaspur) representative of subtropical area in central India. In order to assess the metal deposition on plant, concentrations of six target metals (i.e., Fe, Mn, Pb, Cu, Cd, and Cr) in both plant leaf and dust (deposited on its surface) samples were measured from six different sites. Metal concentrations in dust samples were found on the order of Fe>Mn> Cr>Pb>Cu>Cd. In contrast, the concentration of metals in plant leaves were seen on the order of Fe>Mn>Cr>Cd>Cu>Pb. As such, Cd showed significantly high concentration in leaves relative to their corresponding dust samples. A high accumulation potential for Fe and Cd was seen from Butea monosperma, while Mn and Pb were accumulated noticeably in Pongamia pinnata and Butea monosperma. Likewise, Cr and Cu were enriched in Calotropis procera, Alstonia scholaris, and Butea monosperma. The overall results of our study suggest that the foliar uptake pattern should vary considerably by an interactive role between plant and metal types.

• Journal of Plant Biotechnology
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• v.33 no.4
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• pp.263-270
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• 2006

Pongamia pinnata Pierre is a tree legume, having potential in production of raw material for biodiesel. A protocol for in wk propagation of this plant was standardized using seedling explants. Growth regulators (GR) including gibberellic acid $(GA_3),\;N^6-benzylaminopurine(BA)$, thidiazuron (TDZ), and Adenine sulphate (Ads) were tested for optimum germination of seeds. Removal of seed coat prior to germination, controlled fungal growth partially but enhanced bacterial growth. Antibiotic cefotaxime was ineffective in controlling bacterial contamination. Seedling derived nodal explants and cotyledon nodes with attached cotyledons were excised and cultured for induction of shoots. Optimum sprouting and multiplication of shoot buds were obtained in MS medium supplemented with $8.88{\mu}M$ BA. These buds differentiated and rooted on medium devoid of GR. Optimum growth of Pongamia seedling was obtained in cotton plugged culture vessels. Reculturing of the cotyledon node explants produced more shoots from the same site. This process of removing shoots and reculturing of cotyledon node was followed for eight passages yielding 4 to 8 shoots in each cycle. The shoots (75%) rooted on half strength MS basal medium supplemented with 0.22% charcoal. All plants survived on transfer to soil. This is the first report on in vitro regeneration of Pongamia pinnata. This report demonstrates the possibility of coupling more than one parameter in single experiment to hasten the process of standardization. The process of cycling the nodal explant repeatedly for production of large number of shoots from single meristem may find application in genetic transformation experiments wherein meristems are used for transformation.