• Journal of Korean Society of Forest Science
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• v.102 no.2
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• pp.239-246
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• 2013

This study observed changes of understory vegetation to evaluate the role of forest aggregate after 15% aggregated retention harvest in mature oak forest (> 100 years) in Gyungsangbukdo Bonghwagun in 2010 and 2011. Spontaneous responses of understory vegetation cover (%), species richness, abundance of plant growth forms (herbaceous and woody plants), and overall attributes (by Ordination analysis) were estimated in aggregate area (0.15 and n=36) and clear cut area (0.85 and n=192) in experimental site and control site (1 and n=300). Based on ordination analysis, overall change of species composition in aggregated sites were relatively lower than in harvest area. Right after treatment, total cover of cutted area slightly decreased from 15.6% to 14.7%, and species richness increased from 14 species to 22 species. Cover and richness in the both of aggregate and control sites increased. In plant growth forms, 15% aggregate harvest revealed positive effects on the abundance (cover and richness) of herbaceous plants than woody group. After retention treatment, overall, edge effect likely played major component of vegetation changes in aggregate forest and in harvested area, mechanical damage from harvest operation and change of forest structure by clear cutting were critical. As pre-treatment data, which are rare in ecological studies in Korea, were critical for interpretation between patterns that may have arisen from spatial distributions in the original forest, our experimental design have higher opportunity for long term monitoring on the effect of forest aggregate and vegetation regeneration in clear cutted area.

• Korean Journal of Medicinal Crop Science
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• v.1 no.2
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• pp.129-136
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• 1993

For the clonal proliferation of Gentiana scabra Bunge. which is one of the medicinaland ornamental plant, establishment multiplication of shoot through tissue culture technique and transplantation into soil were carried out. The shoot proliferation increased on the MS medium containing 0.5mg/l NAA and 0.5mg/l BAP. Optimum pH for shoot growth was pH 5.9, consequently MS medium supplemented with 2g/l activated charcoal was most effective for plant growth. There are two types of somaclonal variants, tall type was 63% and dwarf type was 37%.

• Korean Journal of Plant Tissue Culture
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• v.27 no.3
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• pp.213-217
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• 2000

To investigate the cucumber regeneration from embryogenic calli, shoot tips of aseptically-grown cucumber seedlings were used as explants for establishing tissue cultures. Growth and differentiation of callus were studied by using Murashige and Skoog's (MS) medium containing 0.5 to 2 mg/L 2,4-D. Plantlets were induced from shoot tip culture on the plant growth regulators-free MS medium. Non-embryogenic calli and viscous calli were induced on the medium supplemented with 0.5 to 2 mg/L 2,4-D, but embryogenic callus was not induced on the same medium. Segments (ca. 5∼10 mm) of aseptically-grown hypocotyl from five to seven days old seedlings after germination were placed on MS medium supplemented with 1 mg/L 2,4-D for 50 days. Embryogenic calli and embryoids were induced only from the seedlings grown in dark condition, and hypocotyl was placed on the media explanted in light condition. Foully-five point one percent of white fragile calli and 0.6% yellowish compact calli formed roots. Yellowish callus lines were investigated to have a considerably higher concentration of crude proteins than white callus lines. Plantlets derived from embryogenic calli or embryoids have been transferred to pots containing sterile vermiculite and perlite. Normal fruits were harvested from nutrient culture on aggregated hydroponics in the F-clean house.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.4
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• pp.425-430
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• 1983

Rice (Oryza sativa L.) anthers collected from different genotypes were cultured to investigate the priority to increase callus production rate among several factors affecting on callus formation. Rice varieties, F$_1$ hybrids and F$_2$ plants differed greatly in their abilities to produce callus from anthers, and it was confirmed that the culturability of rice anther was a heritable characteristic. Cold shock treatment before plating anthers promoted callus formation rate, but combined effect with genotype having high culturability was more significant. The response to sucrose concentration in culture medium in callus induction rate was different between rice genotypes, but combined effect with genotype was not significant. Single supplement of NAA to R$_2$ medium increased callus production rate remarkably only in rice genotype having high culturability. Conclusively selection of genotypes is most important to increase callus initiation frequency from rice anthers.

• Korean Journal of Plant Resources
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• v.21 no.5
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• pp.368-373
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• 2008

In order to investigate the effects of low temperature pretreatment of floral bud and plant growth regulators on anther-derived callus and shoot differentiation, anthers were cultured on 1/2 MS medium supplemented with 2,4-D, NAA, BA and TDZ. This plant depends on the plant growth regulators, for these anthers couldn't respond on 1/2 MS medium without plant growth regulators. 2,4-D was a prerequisite substance in this experiment, especially 52.6% of callus formation on MS medium with 2.0mg/L 2,4-D alone. However, the optimum medium was on 1/2 MS medium with 0.1 mg/L 2,4-D and 1.0mg/L BA for continuous growth and shoot differentiation from the anther. Calli derived from on MS medium with 2.0mg/L 2,4-D transferred to the 1/2MS medium with TDZ and BA. TDZ were less superior to BA, only one anther could produce shoot on MS media with 1.0mg/L TDZ. On the other hand, when the calli transferred to the medium with 3.0mg/L BA, adventitious shoots were proliferated, subsequently, regenerated shoots elongated from the embryogenic calli. After floral buds of one week before anthesis were incubated at $5^{\circ}C$ refrigerator for eight or fifteen days, anthers seperated from floral buds were cultured on 1/2MS medium supplemented with 0.1mg/L 2,4-D and 1.0mg/L BA. Callusing and shoot differentiation on anthers from treated at $5^{\circ}C$ for eight days were more effective than those of fifteen days or control.

• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.484-489
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• 2017

Lilies as cut flowers are one of the most popular ornamental plants in South Korea. It is necessary to develop lily cultivars with high qualities. Therefore, highly efficient propagation systems are needed following release of elite cultivars. In this study, we used taurine treatment to improve the growth conditions including shoot and bulb formation, fresh weight gain, and reduction of rooting and browning. We experimentally evaluated the effect of taurine as a growth stimulator, at concentrations of 0, 2.5, 5, 10, 15 and 20 mg/l. The results showed that 20 mg of taurine enhanced shoot formation by 85% and increased fresh weight 5.5-fold, which was higher than the approximately four-fold increase in the control. In addition, multiple bulb formation rate was increased by 80% and rooting by 82% following exposure to 20 mg/l of taurine. The efficiency of taurine treatment was higher than that of control with 50% multiple bulb formation rate and 60% rooting rate. The browning was 10.6% at 2.5 mg/l of taurine when compared with 0.8% at 20 mg/l. Taurine showed a positive effect on the overall growth of lily plants in terms of increased fresh weight, shoot formation rate, rooting, and formation of multiple bulbs, indicating that taurine can be used as an alternative to amino acids or as an antioxidant such as citrate and vitamin C in plant tissue culture.

• Korean Chemical Engineering Research
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• v.50 no.2
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• pp.365-370
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• 2012

In this study, Economic analysis of supercritical coal-fired power plant with $CO_2$ capture process was performed. For this purpose, chemical absorption method using amine solvent, which is commercially available and most suitable for existing thermal power plant, was studied. For the evaluation of the economic analysis of coal-fired power plant with post-combustion $CO_2$ capture process in Korea, energy penalty after $CO_2$ capture was calculated using the power equivalent factor suggested by Bolland et al. And the overnight cost of power plant (or cost of plant construction) and the operation cost reported by the IEA (International Energy Agency) were used. Based on chemical absorption method using a amine solvent and 3.31 GJ/$tonCO_2$ as a regeneration energy in the stripper, the net power efficiency was reduced from 41.0% (without $CO_2$ capture) to 31.6% (with $CO_2$ capture) and the levelized cost of electricity was increased from 45.5 USD/MWh (Reference case, without $CO_2$ capture) to 73.9 USD/MWh (With $CO_2$ capture) and the cost of $CO_2$ avoided was estimated as 41.3 USD/$tonCO_2$.

• Korean Journal of Weed Science
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• v.18 no.3
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• pp.205-213
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• 1998

This experiment was carried out to produce herbicide resistant potatoes hawing only chimeric phosphinothricin acetyltransferase (PAT) genes without using antibiotic selectable marker. The pDY502 vector having only PAT gene was reconstructed for transformation of potato. The reconstructed vector was introduced to Agrobacterium tumefaciens MP90 disarmed, and they were used for potato transformation. Hormonal requirement for plant regeneration from leaves and stem explants of potato was investigated. From this experiment, MS medium treated with IBA 0.1 mg/L + BA 0.5 mg/L was the best for potato regeneration, and the ratio of shoot regeneration was 54% for leaf and 46% for stem in that condition. For transformation, explants of potato leaves and stems were cocultured with A. tumefaciens MP90 containing reconstructed vector harvoring only PAT gene. When the potato explants were placed on various concentrations of bialaphos and all the potato explants were dead on medium with over 5.0mg/L bialaphos. By this selection methods, the explants cocultured with Agrobacterium produced the putative transgenic shoots on medium with 5mg/L bialaphos treatment after 3-4 weeks. Second selection was performed by transferring the shoot tips of putative transgenic to medium containing 20mg/L of bialaphos. The shoot tips grew well on the second selection medium, indicating the production of successful transgenic plants. But normal shoots were dead in same cytotoxic medium. Incorporation of the PAT gene into transgenic potatos were confirmed by PCR analysis of DNA and Southern hybridization. These results show that the PAT gene can serve as a selectable marker and herbicide resistant genes for transformation of potato.

• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.43-49
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• 2018

The species of forage crops used in this study were Italian ryegrass (cv. Kowenery), sorghum (cv. SX17), proso millet (cv. domestic) and Japanese millet (cv. Jeju). The plant height of the summer crops was the highest at the dough stage. The dry matter yield of Italian ryegrass was 902.7 kg per 10 a. The dry matter yield of the winter crop and sorghum was 11,985 kg when harvested at the dough stage rather than at the first and second harvests. The proso and Japanese millet also had higher yields for dry matter during the dough stage rather than during heading and regeneration. The acid detergent fiber (ADF) content of Sorghum was lower than that of the first and second harvest; however, the proso and Japanese millet had a higher ADF content at the dough stage. The neutral detergent fiber (NDF) content was higher at the dough stage than at the first and second harvest, and the crude protein content was also lower at the dough stage than at the first and second harvest. The crude protein production for the dry matter yield was about 84 kg in Sorghum when harvested at the dough stage. Proso millet showed no difference for the crude protein production at the heading and dough stage while the Japanese millet had a higher crude protein production. There were no differences in the total digestible nutrients (TDN) content for the three crops according to the harvesting time. Therefore, if Sorghum and Proso and Japanese millet are to be combined with Italian ryegrass, it is better to harvest them at the dough stage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.4
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• pp.498-504
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• 2014

Potato virus Y (PVY) and potato leafroll virus (PLRV) are among the most damaging potato viruses and prevalent in most potato growing areas. In this study, cryopreservation was used to eradicate PVY and PLRV using two cryogenic methods. Potato shoot tips proliferated in vitro were cryopreserved through droplet-vitrification and encapsulation-vitrification using plant vitrification solution 2 (PVS2; 30% glycerol + 15% dimethyl sulfoxide + 15.0% ethylene glycol + 13.7% sucrose) and modified PVS2. Both cryogenic procedures produced similar rates of survival and regrowth, which were lower than those from shoot tip culture alone. The health status of plantlets regenerated from shoot tip culture alone and cryopreservation was checked by reverse transcription-polymerase chain reaction. The frequency of virus-free plants regenerated directly from highly proliferating shoot tips reached 42.3% and 48.6% for PVY and PLRV, respectively. In comparison, the frequency of PVY and PLRV eradication after cryopreservation was 91.3~99.7% following shoot-tip culture. The highest cryopreserved shoot tip regeneration rate was observed when shoot tips were 1.0~1.5 mm in length, but virus eradication rates were very similar (96.4~99.7%), regardless of shoot tip size. This efficient cryotherapy protocol developed to eliminate viruses can also be used to prepare potato material for safe long-term preservation and the production of virus-free plants.