• Journal of Life Science
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• v.24 no.5
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• pp.558-566
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• 2014

Growth induced by cadmium (Cd) and ethylsalicylic acid (ESA) and the effect of ESA on rubisco/rubisco activase were studied in tobacco. The effect of denaturants on rubisco/rubisco activase was also investigated. In order to determine optimal concentration of ESA for growth of tobacco, tobacco was treated with $10^{-6}$-10 mM. It was found that its growth was the highest at $10^{-4}$ mM ESA. In the experiment using control, Cd treated group, ESA treated group, and Cd and ESA mixture group, ESA alone showed the highest growth and Cd showed the lowest growth. Cd treated group was the lowest in both rubisco/rubisco activase content and activity. ESA reduced the rubisco/rubisco activase content, but increased their activity. The activity of rubisco was inhibited by treating L-cysteine, urea, thiourea, ${\beta}$-mercaptoethanol, and EDTA other than guanidine-HCl in control group. L-cysteine, urea, thiourea, and guanidine-HCl treatments showed no change, but ${\beta}$-mercaptoethanol and EDTA increased rubisco activase activity. In conclusion, ESA inhibited the content of rubisco and promoted its activity, whereas promoted the content of rubisco activase and inhibited its activity. In addition, the content and activity of rubisco and rubisco activase inhibited by Cd were recovered by ESA. The activity of rubisco and rubisco activase by Cd and ESA was inhibited by the denaturant and the recovery of ESA inhibited by Cd was lost by the denaturant.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.5
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• pp.1485-1489
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• 2004

Panax ginseng is the one of best famous phytochemical plant in the world and it's various positive effects such as antioxidant, regulation of immunity are very well known. In this study, we investigated primary the cell viability and morphological change and secondary an antioxidative effect and liver function improvement of extract from Ginseng folium and stem in CCl4 intoxicated rats. The NCTC cell line were used for cell viability and sirius red staining before the animal experiment. The female Sprague-Dawley rats (90-100g) were divided into 3 groups (Normal, AC: CCl₄ treated group, GFS: CCl₄+ extract of Ginseng folium and stem treated group) and acute liver damage was developed by one time administration of CCl₄ mixture (0.5㎖/rat). The liver tissue and sera were collected and used for quantitative measurement of enzyme activity (AST, ALT, ALP, BUN), MDA and Hyp. As a result, cell viability in GFS treated group (in concentration of 3.33-33.33㎎ GFS/200㎕ medium) was 180.9-241.0% significantly and dose dependently higher than in control group. And potential state of cell growth and differentiation and no criteria of cytoplasm lysis and nucleus breaking were observed in control and GFS group. The parameters of liver function (AST and ALP) in sera of GFS group showed significantly 93% and 67.6% lower than AC group (p<0.005-0.05). And the level of ALT and BUN showed fast similar in AC group and GFS group. The concentration of MDA in liver was decreased 576.5% significantly in GFS group when compared with AC group (p<0.005). The content of Hyp in GFS group is merely lower than in AC group. In conclusion, the water extract of Ginseng folium and stem such as Ginseng radix may be possessed the antioxidative effect and improvement of liver function in CCl₄ intoxicated rats.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.12
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• pp.1287-1292
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• 2006

Constructed wetlands are well known as highly efficient system to treat wastewater from different sources. Among the constructed wetlands, upflow types of constructed wetlands have become a common selection of wastewater during the last decade. We conducted a pilot scale study at peen house on treating potential of nutrients by upflow vegetated filter(UVF) pilot wetland which was combined with hydrodynamic separator and used the cattail plant(Typha angustifolia), and operated with artificial nutrients influent. This study evaluate the performances of upflow vegetated fille, in removal of nutrients. The objectives of this study were two-fold: (i) to evaluate the nutrients removal performance of pilot-scale upflow vegetated filter, filled with a mixture of perlite and soil media and planted with cattails and (ii) to design of scale-up upflow vegetated filter using Froude number. Results indicated that, under the condition of the ranges of hydraulic surface load rate were $22.7{\pm}9.6\;m^3/m^2/day$, the average removal of $COD_{Mn}$, and TN, TP were 57.5%, 40.0% and 41.5%, respectively. Computational fluid dynamics, FLUENT 6.0 program was used to predict the distribution of velocity in UVF and hydrodynamic separator. Full scale UVF was designed using the Froude number scale-up method that was assumed geomertic similarity between model and prototype. Result shows that the UVF with 3 m diameter has capacity of design sewage flowrate 75 $m^3/day$.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.11
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• pp.1075-1086
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• 2008

In order to investigate the applicability and suitability of the industrial by-products to apply mono-layer cover system for non-sanitary landfill sites, 6 different industrial by-products, such as construction waste, bottom ash, gypsum, blast furnace and steel manufacture slags, and stone powder sludge, were evaluated. Various physicochemical and hydrodynamic properties of the industrial byproducts were investigated. The environmental safety was monitored using batch and long-term leaching tests as well. In addition, the flexibility of plants was observed by cultivating them in the industrial by-products. The results for physicochemical properties indicate that most of the materials considered appeared to be suitable for landfill cover. Particularly, the concentration levels of hazardous elements regulated by the Korean Law for Waste Management did not exceed the regulatory limits in all target materials. In addition, the concentrations of regulated elements for the Korean Soil Conservation Law were examined below the regulatory limits in most of materials considered, except for the stone powder sludge. The results of batch and long-term experiments showed bottom ash and construction waste were the most suitable materials for landfill cover among the industrial by-products considered. The results of plant studies indicate that the bottom ash among industrial by-products considered was most effective in developing vegetation on landfill site, showing fast germination and large growth index. At the final covering system made of mixture of soil and bottom ash, the optimum application rate of farmyard manure was observed to be 40-50 Mg/ha.

• Korean Journal of Weed Science
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• v.18 no.4
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• pp.341-347
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• 1998

This study was carried out to investigate the effect of U-Granule formulation of molinate(S-ethylhexaphdro-1H-azepine-1-carbothioate) mixtures in green house and paddy field, Five minutes were taken for U-Granule to spread out 7m in irrigated water of direct seeded on flooded paddy surface. The concentration of active ingredient of molinate in molinate U-Granule application was similar to molinate+simetryn U-Granule application. But weeding effect of molinate+simetryn U-Granule on Echinochlor crus-galli was 23% higher Than single application of molinate at 7m from application point. Bioefficacy of molinate+simetryn+imazosulfuron U-Granule on control of Echinochloa crus-galli was higher than that of molinzte+imazosulfuton U-Granule, but those effect on Eleochairs kuroguwai was not significantly different. Only slight rice phytotoxicity was observed at 5m and 2m from application point of U-Granule molinate+simetryn+imazosulfuron and molinate+imazosulfuron, respectively. So it is concluded that there is little phytotoxicity problem in practical application of U-Granule of molinate mixtures.

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.207-216
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• 1995

This study was conducted to establish technologies for plug seedling production using somatic embryos-derived regenerants and their seeds in Bupleurum falcatum L. Among distilled water, GA (0.1mg/l) and putrescine (0.lmg/l) treated to regenerants for acclimatization, GA was most effective to develop shoots and roots, 1/2X MS medium and NAA 0.1mg/l + BA 0. 5mg/l enhanced the growth rates of the regenerants and increased dry weight. Activated charcoal effected to grow markedly leaves and roots of the regenerants at the level of 0.4 %. Regenerants increased their plant height, root length and dry weight at $30^{\circ}C$. Plug seedlings originated from seeds of the tissue culture regenerants showed the maxium growth on the mixture of peatmoss soil (2) and mountain sand (1) .Root length, leaf area and dry weight of plug seedlings increased significantly when No.1, 2 and 3 of Wondergrow solution were mixed in the ratio of 1.3 - 0.9 - 0.1. Light supplement (4%) and high tem­perature $(30^{\circ}C)$ promoted the growth of plug seedlings as well as dry weight. Ninety days seedlings were more vigorous and adaptable for transplanting than other seedlings.

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.246-250
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• 1995

Scions from the one- and two-years old and the nodes of Ligusticum chuanxiong stem were used to study the rooting ability with the treatment of plant growth regulators planted to different bed soils as the alternative propagation for the present method by rhizome. The rooting ratio in the scion from the one- and the two-years old plants showed any difference, but that was ranged from 94% to 100% in the first node and from 56% to 64% in the second node from the bottom of the stem. The scion of first node from both the plants showed more than 90% in the root ratio and 16 in the number of rooting and growed more than 31cm in the root length. The Rootone-F and 250ppm of NAA were most effective for the promotion of rooting. Rooting ratios in the sand and in the mixture of vermiculite and perlite as bed soils were about 18%, but that in the cultured soil considerably decreased.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.21
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• pp.9319-9325
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• 2014

Alkaloids are the most extensively featured compounds of natural anti-tumor herbs, which have attracted much attention in pharmaceutical research. In our previous studies, a mixture of major three alkaloid components (5, 6-dihydrobicolorine, 7-deoxy-trans-dihydronarciclasine, littoraline) from Hymenocallis littoralis were extracted, analyzed and designated as AHL. In this paper, AHL extracts were added to human liver hepatocellular cells HepG-2, human gastric cancer cell SGC-7901, human breast adenocarcinoma cell MCF-7 and human umbilical vein endothelial cell EVC-304, to screen one or more AHL-sensitive tumor cell. Among these cells, HepG-2 was the most sensitive to AHL treatment, a very low dose ($0.8{\mu}g/ml$) significantly inhibiting proliferation. The non-tumor cell EVC-304, however, was not apparently affected. Effect of AHL on HepG-2 cells was then explored. We found that the AHL could cause HepG-2 cycle arrest at G2/M checkpoint, induce apoptosis, and interrupt polymerization of microtubules. In addition, expression of two cell cycle-regulated proteins, CyclinB1 and CDK1, was up-regulated upon AHL treatment. Up-regulation of the Fas, Fas ligand, Caspase-8 and Caspase-3 was observed as well, which might imply roles for the Fas/FsaL signaling pathway in the AHL-induced apoptosis of HepG-2 cells.

• Proceedings of the Korean Society of Community Living Science Conference
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• 2009.09a
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• pp.95-95
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• 2009

우리나라를 비롯한 동양문화권에서는 천연 약용식물자원의 이용을 통하여 전통적으로 지역 보건향상을 도모해 왔으며 최근 성인병과 난치병 해결을 위한 대체의학에 대한 관심이 증가하면서 약용식물자원을 이용한 건강기능식품 분야가 주목되고 있다. 약용식물자원은 민간에서 약선음식 등에 널리 활용되고 있지만 원료 농산물을 그대로 이용하는 수준으로 광범위한 적용성을 가지는 약선식품용 소재화 기술개발은 약용식물자원의 소비촉진에 크게 기여할 것으로 기대된다. 본 연구에서는 약선식품용 식품소재 개발의 일환으로 약용식물자원의 배합비와 추출물의 제형이 품질특성에 미치는 영향을 조사하였다. 스트레스 경감기능을 보유하는 약용식물자원은 고문헌에 기초하여 선정하였으며 약선원리에 따라 소정의 조건으로 3종(CLP 1, CLP 2, CLP 3) 배합비를 설정하였다. 추출물은 온도를 달리한 열수에 추출하고 유동Ext, 분무건조분말 및 과립으로 제형화 하였고 품질특성은 폴리페놀함량, 용해도, 투과도 및 색도특성을 분석하였다. 약용식물자원 혼합 추출물의 수율은 CLP 2가 65%로 가장 많았으며 1차 추출에서 76%의 수율을 보였고 추출시간대비 수율은 상업적 추출의 경우가 가장 높았다. 유동 Ext의 폴리페놀함량은 CLP 2가 g 당 11~13 mg을 함유하여 가장 많았고 CLP1과 CLP 2는 동등한 수준을 나타냈다. 유동 Ext는 99%의 용해도를 보였으며 투과도는 CLP 3가 52~68%로 가장 낮았으며 추출조건에 따라 색도가 차이가 있었다. 분말형태의 경우 폴리페놀 함량은 분무건조로 제조한 CLIP 2가 15.40mg/g으로 가장 많았다. 부형제로 과립화한 CLP 3가 g당 1.7 mg으로 가장 적었다. 분무건조분말은 98~99%의 용해도를 보였으며 부형제를 사용하여 분무건조하거나 과립화한 시료는 투과도와 명도가 높았으며 적색도와 황색도 및 갈색도가 낮았다. 이상의 결과는 약용식물자원 혼합 추출물은 원료의 조합비와 추출물의 제형은 식품소재화에 따른 품질특성의 영향인자로 작용함을 나타낸다.

• Applied Chemistry for Engineering
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• v.29 no.1
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• pp.10-17
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• 2018

POU (point of use) scrubbers were applied for the treatment of waste gases including PFCs (perfluorocompounds) exhausted from the CVD (chemical vapor deposition), etching, and cleaning processes of semiconductor and display manufacturing plant. The GWP (global warming potential) and atmosphere lifetime of PFCs are known to be a few thousands higher than that of $CO_2$, and extremely high temperature more than 3,000 K is required to thermally decompose PFCs. Therefore, POU gas scrubbers based on the thermal plasma technology were developed for the effective control of PFCs and industrial application of the technology. The thermal plasma technology encompasses the generation of powerful plasma via the optimization of the plasma torch, a highly stable power supply, and the matching technique between two components. In addition, the effective mixture of the high temperature plasma and waste gases was also necessary for the highly efficient abatement of PFCs. The purpose of this paper was to provide not only a useful technical information of the post-treatment process for the waste gas scrubbing but also a short perspective on R&D of POU plasma gas scrubbers.