• 식물병연구
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• 제24권1호
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• pp.52-58
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• 2018

배를 유기재배하는 과원에서 문제가 되는 주요 병을 효과적으로 방제할 수 있는 친환경유기농자재 살포프로그램을 도출하기 위하여 친환경유기농자재 살포에 따른 검은별무늬병과 붉은별무늬병에 대한 방제효과를 관행재배와 비교 검정하였다. 석회유황합제와 석회보르도액을 10회 살포한 친환경방제구에서는 관행재배과원에 비해서 잎에 발생하는 검은별무늬병과 붉은별무늬병에 대해 40% 이상의 방제효과를 증대시켰다. 석회유황합제만을 기상조건을 고려하여 강우 전후에 8회 살포한 친환경방제구에서도 관행재배과원에 비해서 과실에 발생하는 검은별무늬병에 대해 30% 이상의 방제효과를 증대시켰다. 배 유기재배시 기상조건을 고려하여 친환경유기농자재 살포시기를 조절하는 친환경방제로 주요 병의 효율적 관리가 가능하리라 전망된다.

• Journal of Plant Biotechnology
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• 제34권2호
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• pp.161-166
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• 2007

In vitro shoot regeneration and multiple shoot induction has been obtained from the stolen node explants in Eremochloa ophiuroides (Munro) Hack. The highest number of shoots ($10.66{\pm}0.21$) was observed from initial explants after one month culture duration on Murashige and Skoog (MS) medium containing 6-benzyladenine (BA: 0.5 mg/l). First generation shoot was excised and sub-cultured on the same fresh media for further multiplication of shoots. An enhanced number of second round shoots ($15.33{\pm}0.21$) was obtained compared to the initial culture media containing BA (0.5 mg/l). The number of shoots/stolon node was higher among all the concentrations of BA than kinetin (KN). In vitro regenerated shoots were successfully rooted in the phytohormone free MS medium. Plantlets generated with roots were transferred to pots containing compound mixture of soil and kept in green house conditions. Acclimatized plants showed 100% survival rate with normal morphology in green house conditions. The present study demonstrates the effect of explant and different plant growth regulators towards in vitro response in E. ophiuroides. Moreover, the study reveals the effect of cytokinin on induction of shoot number per stolen node explant in E. ophiuroides.

• 한국축산식품학회지
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• 제38권3호
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• pp.442-450
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• 2018

Liquid egg products can be contaminated with Salmonella spp. during processing. A predictive model for the growth of Salmonella spp. in unpasteurized liquid eggs was developed and validated. Liquid whole egg, liquid yolk, and liquid egg white samples were prepared and inoculated with Salmonella mixture (approximately 3 Log CFU/mL) containing five serovars (S. Bareilly, S. Richmond, S. Typhimurium monophasic, S. Enteritidis, and S. Gallinarum). Salmonella growth data at isothermal temperatures (5, 10, 15, 20, 25, 30, 35, and $40^{\circ}C$) was collected by 960 h. The population of Salmonella in liquid whole egg and egg yolk increased at above $10^{\circ}C$, while Salmonella in egg white did not proliferate at all temperature. These results demonstrate that there is a difference in the growth of Salmonella depending on the types of liquid eggs (egg yolk, egg white, liquid whole egg) and storage temperature. To fit the growth data of Salmonella in liquid whole egg and egg yolk, Baranyi model was used as the primary model and the maximum growth rate and lag phase duration for each temperature were determined. A secondary model was developed with maximum growth rate as a function of temperature. The model performance measures, bias factor ($B_f$, 0.96-0.99) and $r^2$ (0.96-0.99) indicated good fit for both primary and secondary models. In conclusion, it is thought that the growth model can be used usefully to predict Salmonella spp. growth in various types of unpasteurized liquid eggs when those are exposed to various temperature and time conditions during the processing.

• 식물조직배양학회지
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• 제24권5호
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• pp.263-268
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• 1997

다래(A. arguta)의 절간조직을 절편체로하여 식물생장조절제의 종류와 광조건이 shoot 형성에 미치는 영향을 조사하였으며 아울러 기내발근을 통하여 다래로부터 완전한 식물체를 재분화시킬 수 있는 실험체제를 확립하였다. 절간절편체는 3.0 mg/L의 zeatin이 단독첨가된 배지에 암배양한 경우 62.5%로 높은 shoot 형성률을 나타내었다. 0.5mg/L NAA가 포함된 WPM 배지에 4주 동안 배양하여 발근시킨 식물체는 vermiculite와 perlite 가 1:1(v/v)로 혼합된 배양토에 1주간 순화시킨 후 일반토양에 이식한 결과, 완전한 식물체로 생장시킬 수 있었다. 다래의 절간절편체를 shoot 유도배지에 배양하여 shoot의 형성과정을 발달단계별로 조직학적인 측면에서 관찰한 결과, 배양초기에는 신장되거나 모양이 다양한 무정형의 세포가 대부분이었으나 배양이 진전됨에 따라 세포의 크기가 작고 세포질이 충만한 분열조직군이 형성되면서 이들로부터 장차 shoot로 발달될 shoot primordia가 형성되는 것을 관찰할 수 있었다.

• 한국건설순환자원학회논문집
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• 제4권2호
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• pp.165-171
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• 2016

본 연구에서는 에코 프리캐스트 콘크리트 제품 개발을 위한 기초 연구로서 박테리아를 흡착한 팽창질석 기반의 친생태 모르타르를 개발하였으며, 압축강도 발현 및 생태환경영향 평가를 실시하였다. 경화된 모르타르의 극건조 환경 및 강알칼리성 환경에서 생장이 가능하며, 식물생장성 증대 효과를 갖는 Bacillus alcalophilus와 Rhodoblastus acidophilus를 분리 및 배양하였다. 배양이 완료된 박테리아는 생장처 제공을 위한 재료로서 선택된 팽창질석에 흡착하였으며, 이를 배합시 골재의 체적 대비 혼입하여 모르타르를 제작하였다. 평가 결과 친생태 모르타르는 COD 및 T-N 수질정화능력 및 식물생장증대에 효과를 보였다. 모르타르의 압축강도 발현 및 비용적 측면을 고려했을 때, 박테리아 기반 팽창질석의 최적 치환율은 최대 10% 이하가 추천될 수 있었다.

• 한국환경보건학회지
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• 제31권4호
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• pp.332-339
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• 2005

The environmental regulations in the world has been reinforced and many nations has devoted themselves to the development of cost-effective technology. Selective catalyst reduction(SCR) and selective non-catalyst reduction (SNCR) processes are mainly used to treat nitrogen oxidants generated from fossil-fuel combustion. One of these typical technologies for reduction of do-NOx is SNCR process has increased continuously because of the low cost for building and maintenance. Nevertheless the researches on the application to real scale plant by the reductant like Urea are rarely studied. In this paper, an experimental investigations were performed on the SNCR process in the industrial waste incineration plant. With no reducing agent, the concentration of NOx stayed in around 180 ppm $(O_2\;12\%)$ with the exhausting temperature of $950^{\circ}C$ and changed within the range of 20 ppm to remain relatively consistent. When $10\;wt\%)$ of a solution was added, the efficiency of denitrification reached above $61.4\%$ with the NSR of 2.0 and the exhausting temperature of $950^{\circ}C.$ When the concentration of the urea solution was set to $10\;wt\%$ and the sprinkling to four nozzles, the reaction temperature was reduced to about $50~100^{\circ}C$ with a mixture of $10\;wt\%\;CH_3OH\;and\;5wt\%\;Na_2CO_3$ in $40\;wt\%$ of the solution. The NOx removal efficiency increased to $78.4\%,$ achieving a broader and expansive range of reaction temperatures than the addition of an unmixed pure solution.

• Journal of Ginseng Research
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• 제39권1호
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• pp.81-88
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• 2015

Background: In Changbai Mountains, Panax ginseng (ginseng) was cultivated in a mixture of the humus and albic horizons of albic luvisol in a raised garden with plastic shade. This study aimed to evaluate the impact of ginseng planting on soil characteristics. Methods: The mixed-bed soils were seasonally collected at intervals of 0-5 cm, 5-10 cm, and 10-15 cm for different-aged ginsengs. Soil physico-chemical characteristics were studied using general methods. Aluminum was extracted from the soil solids with $NH_4Cl $(exchangeable Al) and Na-pyrophosphate (organic Al) and was measured with an atomic absorption spectrophotometer. Results: A remarkable decrease in the pH, concentrations of exchangeable calcium, $NH_4^+$, total organic carbon (TOC), and organic Al, as well as a pronounced increase in the bulk density were observed in the different-aged ginseng soils from one spring to the next. The decrease in pH in the ginseng soils was positively correlated with the $NH_4^+$ (r=0.463, p<0.01), exchangeable calcium (r=0.325, p<0.01) and TOC (r= 0.292, p < 0.05) concentrations. The $NO_3^-$ showed remarkable surface accumulation (0-5 cm) in the summer and even more in the autumn but declined considerably the next spring. The exchangeable Al fluctuated from $0.10mg\;g^{-1}$ to $0.50mg\;g^{-1}$ for dry soils, which was positively correlated with the $NO_3^-$ (r=0.401, p < 0.01) and negatively correlated with the TOC (r=-0.329, p < 0.05). The Al saturation varied from 10% to 41% and was higher in the summer and autumn, especially in the 0-5 cmand 5-10 cm layers. Conclusion: Taken together, our study revealed a seasonal shift in soil characteristics in ginseng beds with plastic shade.

• Journal of Ginseng Research
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• 제45권4호
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• pp.519-526
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• 2021

Background: This study aimed to investigate the effect of cold plasma treatment on the improvement of seed germination and surface sterilization of ginseng seeds. Methods: Dehisced ginseng (Panax ginseng) seeds were exposed to dielectric barrier discharge (DBD) plasma operated in argon (Ar) or an argon/oxygen mixture (Ar/O₂), and the resulting germination and surface sterilization were compared with those of an untreated control group. Bacterial and fungal detection assays were performed for plasma-treated ginseng seeds after serial dilution of surface-washed suspensions. The microbial colonies (fungi and bacteria) were classified according to their phenotypical morphologies and identified by molecular analysis. Furthermore, the effect of cold plasma treatment on the in vitro antifungal activity and suppression of Cylindrocarpon destructans in 4-year-old ginseng root discs was investigated. Results: Seeds treated with plasma in Ar or Ar/O₂ exhibited a higher germination rate (%) compared with the untreated controls. Furthermore, the plasma treatment exhibited bactericidal and fungicidal effects on the seed surface, and the latter effect was stronger than the former. In addition, plasma treatment exhibited in vitro antifungal activity against C. destructans and reduced the disease severity (%) of root rot in 4-year-old ginseng root discs. The results demonstrate the stimulatory effect of plasma treatment on seed germination, surface sterilization, and root rot disease suppression in ginseng. Conclusion: The results of this study indicate that the cold plasma treatment can suppress the microbial community on the seed surface root rot in ginseng.

• 한국응용곤충학회지
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• 제50권1호
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• pp.55-63
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• 2011

본 연구는 감귤에 발생하는 해충인 네눈쑥가지나방의 실내 사육용 인공사료를 개발하고자 수행하였다. 인공사료의 주 재료로 옥수수와 메주콩을 조합한 사료에서만 정상적으로 발육하였으며, 옥수수와 메주콩의 전체 함량이 높은 사료에서 유충 생존성이 높았다. 효모와 콜레스테롤을 인공사료에 첨가하였을 때, 유충 발육기간은 짧아지고, 생존율은 높은 것으로 나타났다. 발육기간, 누적 생존율, 산란량에 차이는 인공사료와 감귤 잎을 먹은 개체들 간에 없었다. 최종적으로 네눈쑥가지나방 사육에 최적인 인공사료 조성은 물 1 L당 한천 25 g, 옥수수 100 g, 메주콩 100 g, 효모 30 g, 콜레스테롤 0.5 g, 비타민혼합물(Vanderzant vitamin mix.) 2 g, 염혼합물(Wesson's salt mix.) 2 g, 소르브산 2 g, 메틸파라벤(methyl-4-hydroxybenzoate) 2.5 g, 아스코르브산 2 g이었다. 사육법으로 유충은 플라스틱 원형용기(${\varphi}90{\times}40$ mm) 안에서, 갓 부화 유충부터 4령까지는 40마리씩 집단으로, 5령과 6령은 개체별 사육하였다. 성충들에는 10% 설탕물을 먹이로 하여 플라스틱 상자 안($25{\times}25{\times}25$ cm)에서 암컷 3마리와 수컷 5~6마리가 교미하도록 했고, 난괴는 거즈(눈 크기 1 mm)를 붙인 기름종이에 받았다. 기타 인공사료로 사육한 네눈쑥가지나방의 발육단계별 형태적 특징에 대하여 기술하였다.

• 한국환경과학회지
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• 제19권9호
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• pp.1137-1142
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• 2010

This study proposes a guideline of a green roof system suitable for the local environment by verifying the growth of Zoysia japonica in a shallow, extensive, green roof system under rainfed condition. The experimental soil substrates into which excellent drought tolerance and creeping Z. japonica was planted were made with different soil thicknesses(15cm, 25cm) and soil mixing ratios(SL, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$, $P_4P_4L_2$). The plant height, green coverage ratio, fresh weight, dry weight and chlorophyll contents of Z. japonica were investigated. For the soil thickness of 15cm, the plant height of Z. japonica was significantly as affected by the soil mixing ratio and it was shown in the order SL= $P_4P_4L_2$ < $P_7P_1L_2$ = $P_5P_3L_2$ < $P_6P_2L_2$. For the soil thickness of 25cm, the plant height was increased in order to SL < $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$ < $P_4P_4L_2$. The green coverage ratio was not observed by soil the mixing ratio or soil thickness. However, the green coverage ratio was 86~90% with a good coverage rate overall. The chlorophyll contents of Z. japonica were not significantly affected by the soil mixing ratio in the soil thickness of 15cm, but were higher in the natural soil than in the artificial soil at 25cm soil thickness. The fresh weight and dry weight of Zoysia japonica were heavier in the 25cm thickness than in the 15cm thickness and in the artificial soil mixture than in the natural soil. The result indicated that the growth of Zoysia japonica was more effective in the 25cm soil thickness with artificial soil than in the 15cm soil thickness with natural soil in the green roof system under rainfed condition.