• Title/Summary/Keyword: plant diversity

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Differential Impacts on Bacterial Composition and Abundance in Rhizosphere Compartments between Al-Tolerant and Al-Sensitive Soybean Genotypes in Acidic Soil

  • Wen, Zhong-Ling;Yang, Min-Kai;Fazal, Aliya;Liao, Yong-Hui;Cheng, Lin-Run;Hua, Xiao-Mei;Hu, Dong-Qing;Shi, Ji-Sen;Yang, Rong-Wu;Lu, Gui-Hua;Qi, Jin-Liang;Hong, Zhi;Qian, Qiu-Ping;Yang, Yong-Hua
    • Journal of Microbiology and Biotechnology
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    • v.30 no.8
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    • pp.1169-1179
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    • 2020
  • In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation.

Statistical Analysis of Sewage Plant Operation (하수처리장 운전조건의 통계분석)

  • 이찬형;문경숙
    • Journal of Environmental Science International
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    • v.11 no.1
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    • pp.63-68
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    • 2002
  • In this study, we examined statistical analysis between sewage plant operations parameters and effluent quality We got six components from principle component analysis of the operation parameters and secondary effluent quality. 91.8% of the total variance was explained by the six components. The components were identified in the following order : 1) organic matter removal by aeration basin microbe, 2) settleability on secondary clarifier load, 3) removal of nutrients, 4) microbial number increasement and species diversity, 5) microbial activity in aeration basin, 6) oxidation in aeration basin.

Diversity and Plant Growth Promoting Capacity of Endophytic Fungi Associated with Halophytic Plants from the West Coast of Korea

  • Khalmuratova, Irina;Kim, Hyun;Nam, Yoon-Jong;Oh, Yoosun;Jeong, Min-Ji;Choi, Hye-Rim;You, Young-Hyun;Choo, Yeon-Sik;Lee, In-Jung;Shin, Jae-Ho;Yoon, Hyeokjun;Kim, Jong-Guk
    • Mycobiology
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    • v.43 no.4
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    • pp.373-383
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    • 2015
  • Five halophytic plant species, Suaeda maritima, Limonium tetragonum, Suaeda australis, Phragmites australis, and Suaeda glauca Bunge, which are native to the Muan salt marsh of South Korea, were examined for fungal endophytes by sequencing the internal transcribed spacer (ITS) region containing ITS1, 5.8S rRNA, and ITS2. In total, 160 endophytic fungal strains were isolated and identified from the roots of the 5 plant species. Taxonomically, all 160 strains belonged to the phyla Ascomycota, Basidiomycota, and Zygomycota. The most dominant genus was Fusarium, followed by the genera Penicillium and Alternaria. Subsequently, using 5 statistical methods, the diversity indices of the endophytes were determined at genus level. Among these halophytic plants, P. australis was found to host the greatest diversity of endophytic fungi. Culture filtrates of endophytic fungi were treated to Waito-C rice seedlings for plant growth-promoting effects. The fungal strain Su-3-4-3 isolated from S. glauca Bunge provide the maximum plant length (20.1 cm) in comparison with wild-type Gibberella fujikuroi (19.6 cm). Consequently, chromatographic analysis of the culture filtrate of Su-3-4-3 showed the presence of physiologically active gibberellins, $GA_1$ (0.465 ng/mL), $GA_3$ (1.808 ng/mL) along with other physiologically inactive $GA_9$ (0.054 ng/mL) and $GA_{24}$ (0.044 ng/mL). The fungal isolate Su-3-4-3 was identified as Talaromyces pinophilus.