• Journal of Microbiology and Biotechnology
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• v.31 no.11
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• pp.1526-1532
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• 2021

Suaeda australis, Phragmites australis, Suaeda maritima, Suaeda glauca Bunge, and Limonium tetragonum in the Seocheon salt marsh on the west coast of the Korean Penincula were sampled in order to identify the endophytes inhabiting the roots. A total of 128 endophytic fungal isolates belonging to 31 different genera were identified using the fungal internal transcribed spacer (ITS) regions and the 5.8S ribosomal RNA gene. Fusarium, Paraconiothyrium and Alternaria were the most commonly isolated genera in the plant root samples. Various diversity indicators were used to assess the diversity of the isolated fungi. Pure cultures containing each of the 128 endophytic fungi, respectively, were tested for the plant growth-promoting abilities of the fungus on Waito-C rice germinals. The culture filtrate of the isolate Lt-1-3-3 significantly increased the growth of shoots compared to the shoots treated with the control. Lt-1-3-3 culture filtrate was analyzed and showed the presence of gibberellins (GA₁ 2.487 ng/ml, GA₃ 2.592 ng/ml, GA₉ 3.998, and GA₂₄ 6.191 ng/ml). The culture filtrate from the Lt-1-3-3 fungal isolate produced greater amounts of GA₉ and GA₂₄ than the wild-type Gibberella fujikuroi, a fungus known to produce large amounts of gibberellins. By the molecular analysis, fungal isolate Lt-1-3-3 was identified as Gibberella intermedia, with 100% similarity.

• Korean Journal of Agricultural Science
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• v.38 no.2
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• pp.325-330
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• 2011

In order to revive the ecological function of degraded rivers, a total restoration plan for riverbeds and riparians needs to be developed. Previous evaluations for rivers were mainly focused on the river's physical structures. Therefore, this research has developed indicators to evaluate a riparian restoration considering biodiversity. Through literature and previous cases review, 4 fields and 13 indicators are selected for the evaluation. Four fields are biodiversity, habitat diversity, connectivity and habitat functionality. In the biodiversity field, 4 indicators of the exuberant extent of herbaceous vegetation and their diversity, the exuberant extent of shrub and woody plants and their diversity, the number of plant communities and naturalized plants are included. Habitat diversity are comprised of 4 indicators of the longitudinal continuity of vegetation, the mixture of plant communities, the extent of plant type color fruit abundance and the distribution of vegetation. Connectivity includes 3 indicators of target distribution, the shore slope of low water channels and the extent of artificial embankment materials. Habitat functionality has 2 indicators of the status of food supply plants and the habitat functionality. The value weighting for the fields and indicators has been calculated based on the AHP(Analytic Hierarchy Process) method. 50 experts were surveyed with quantifiable questionnaire, among them 43 experts have more than 10 yesrs experiences in the nature restoration field. The selected and weighted indicators have been tested to the 12 sections in Gap stream located in Daejeon. In conclusion, the indicators are feasible and the selected indicators could be used to establish the direction and objectives of riparian restoration.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.82-82
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• 2017

The production of spring wheat, the major crop in Mongolia, is accounting for 98% of the cultivated area. Collection, conservation and utilization of wheat germplasm resources play an important role in wheat breeding and production in Mongolia. Understanding genetic variability in the existing genebank accessions is important for collection and conservation of wheat germplasms. To determine the genetic diversity and population structure among a representative collection of Mongolian local wheat cultivars and lines, 200 wheat accessions were analyzed with 15 SSR markers distributed throughout the wheat genome. A total of 85 alleles were detected, with 3 to 5 alleles per locus and a mean genetic diversity value of 5.66. The average genetic diversity index was 0.68, with values ranging from 0.37 to 0.80. The 200 Mongolian wheat accessions were divided into two subgroups based on STRUCTURE, un-rooted NJ cluster and principal coordinate analyses. The results from this study will provide important information for future wheat germplasm conservation and improvement programs with Mongolian genebank.

• Korean Journal of Plant Taxonomy
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• v.45 no.1
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• pp.29-35
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• 2015

Sasa borealis (Hack.) Makino & Shibata is widely distributed in South Korea. With amplified fragment length polymorphism (AFLP) markers, we analyzed the genetic diversity of S. borealis to predict and measure the phytogeographical factors of these populations. Relatively high levels of genetic diversity (PPL = 37.2%, h = 0.143, I = 0.205) and genetic differentiation ($G_{ST}$ = 0.324, ${\theta}^B$ = 0.395) were confirmed in populations of S. borealis. Moreover, an analysis of molecular variance (AMOVA) showed that the rate of differentiation among the populations was 47.7%. The results showed that genetic diversity is inversely proportional to the latitude of the S. borealis populations, indicating that the distribution of S. borealis may have extended from lower to higher latitudes. This method of investigating the correlation between genetic diversity and latitude presents critical information for estimating changes in distributions and plant conservation due to climate change.

• Journal of Life Science
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• v.22 no.11
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• pp.1435-1443
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• 2012

The changes of plant communities at Angol valley and Baetgol valley in Geoje-do were studied. Both ${\alpha}$-diversity and ${\gamma}$-diversity decreased with the sizes and degrees of habitat fragmentations. The mean number of winner species decreased with habitat fragmentation. All Quercus species, including Quercus acutissima, significantly declined in importance, while the many introduced species, including Trifolium pretense, all increased in importance. As the proportional change in adult survival rate increased, the absolute value of the bias in the elasticity prediction also increased from 2003 to 2011 at undisturbed forests. However, the bias was low for decreases in disturbed populations. Moran's I values showed overall decreases for habitat fragmentation and for the periods of habitat conversion. Eventually, plant communities, due to urbanization in Goeje-do, might have led to decreased chances of common species when the environment was disturbed.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.105-105
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• 2018

Finger millet, Eleusine coracana Gaertn., is more nutritious than other cereals and millets and widely cultivate in tropical regions of the world. However, status of its genetic diversity remained concealed due to lack of research work in this species. In recent years, microsatellites have become the most used markers for studying population genetic diversity. In present study, genetic diversity and structure of different populations of finger millet from Africa and South Asia was examined at molecular level using newly developed EST-Simple Sequence Repeat (EST-SSR) markers using a total of 1,927 ESTs of Eleusine coracana available in the NCBI database. In total, 46 primers produced 292 alleles in a size range of 100-500 bp and mean Polymorphism Information Content (PIC) and Marker Index (MI) were 0.372 and 1.04, respectively. 46 primers showed polymorphism and 21 primers were identified as having a PIC value above 0.5. Principal coordinates analysis and the dendrogram constructed out of combined data of both markers showed grouping of finger millet accessions to their respective area of collection. The 156 accessions was classified into four groups, such as three groups of Africa collection and one group of Asia. Results of present study can be useful in identifying diverse accessions and management of this plant resource. Moreover, the novel SSR markers developed can be utilized for various genetic analyses in this species in future.

• Korean Journal of Agricultural Science
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• v.48 no.2
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• pp.283-297
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• 2021

Investigation of genetic diversity and molecular characterization in high yielding rice varieties is important for their identification. The experiment was conducted during 2016 - 2017 to analyse the genetic diversity of fifteen high yielding rice varieties in Bangladesh by using random amplification of polymorphic DNA (RAPD) markers. Polymorphism was revealed in 12 RAPD primers out of 30, whereas no other reaction was detected on the remaining 18 primers. The 40 out of 45 bands (88.89%) polymorphics were produced by the primers and ranged from 50 to 100%. The maximum number of polymorphic bands was produced by the primer OPB-18 whereas the lowest number of polymorphic bands belonged to OPC-12. The genetic similarity coefficients were determined with the RAPD data, which ranged from 0.47 to 0.94. The unweighted paired group of arithmetic means (UPGMA) dendrogram presented the studied rice varieties into two major clusters. Moreover, the value of Nei's genetic diversity is 0.26 and the Shanon information index is 0.41. The study produced distinct positions, suggesting that the genotypes were different from each other. The results indicated that these markers could be efficient for comparing the genetic relationships, patterns of variation, and measurement of genetic distance among rice varieties. Considering all of these results, RAPD analysis is found to be an effective tool for estimating the genetic diversity of different rice varieties. The outcomes of this research may contribute to the germplasm data of rice accessions and a future breeding program of rice genotypes.

• The Plant Pathology Journal
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• v.17 no.6
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• pp.383.6-384
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• 2001

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2000.10a
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• pp.30.1-30
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• 2000

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.04a
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• pp.52-53
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• 2002