• Journal of agriculture & life science
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• v.46 no.4
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• pp.113-118
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• 2012

Effects of plant activators on germination of primed pepper seeds were investigated. The percent germination and T50 of primed seeds with 0.1mM ASM were 98% and 0.96 day at the 7 days after seeding, respectively. However, the germination of primed seeds with 0.5mM ASM were suppressed as 17% at the 7 days after seeding. The percent germination of primed seeds with 0.01mM INA were 90% at the 2 days after seeding. However, those of primed seeds by 0.1mM INA were increased as 90% at the 5 days after seeding. The percent germination of primed seeds was not change by treatment of the BABA and JA.

• Journal of Bio-Environment Control
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• v.29 no.2
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• pp.181-188
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• 2020

This study was conducted to identify the optimal conditions for seedling growth in Veronica rotunda var. subintegra (Nakai) T.Yamaz. which a endemic plant and can be development as ornamental plants. We sowed V. rotunda var. subintegra (Nakai) T.Yamaz. seeds, and exposed the seedling was different treatment conditions. We varied soil type and fertilizer concentration, shading ratio, additional fertilizer concentration, pretreatment light exposure and collection time of seeds. We found that seedling growth was good in horticultural substrate (with no additional fertilizer), but mixed soils supplemented with fertilizer inhibited growth, regardless of the fertilizer concentration. In the 55% shading treatment, seedling growth was greater than in the non-shading treatment. High concentration addition of fertilizer (Hyponex) promoted plant growth, in terms of both plant length and fresh weight. Exposure of seeds to a red light-source prior to germination had a greater effect on seedling growth than exposure to other light sources. Seedlings exhibited better growth when grown from seed collected in 2018, rather than 2017.

• Journal of Ecology and Environment
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• v.45 no.4
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• pp.174-181
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• 2021

Background: Euphorbia jolkinii Boiss. is a perennial species native to Jeju Island and the southern coastal area of Korea. Particularly on Jeju Island, the yellow flowers of E. jolkinii Boiss. have a high ornamental value because of their contrast with basalt. This study was conducted to investigate the effects of different temperatures (5, 15, 20, and 25 ℃) and gibberellic acid (GA₃) concentrations (0, 10, 100, or 1000 mg/L) on seed dormancy and germination of E. jolkinii. In addition, we classified the seed dormancy type and compared types with those of other species in the same genus. Results: The number of seeds with viable embryos and endosperms was approximately 66%. The final germination percentages at 5, 15, 20, and 25 ℃ were 51.7%, 83.5%, 2.6%, and 0.0%, respectively. In GA₃ concentration experiments, the final germination percentages of 0, 10, 100, and 1000 mg/L were 83.5%, 91.7%, 79.1%, and 83.4%, respectively, at 15 ℃ conditions, and 0.0%, 6.9%, 13.2%, and 27.3%, respectively, at 25 ℃. Conclusions: Germination improved at temperatures of 15 ℃ or lower. Furthermore, GA₃ treatment effectively reduced germination times. Thus, the seeds of E. jolkinni were classified as having non-deep physiological dormancy.

• Journal of Plant Biotechnology
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• v.30 no.2
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• pp.151-154
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• 2003

This study was conducted to determine the optimal concentrations of plant hormones (2,4-D, picloram, dicamba, NAA, kinetin) and the suitable explants among seeds, hypocotyl, and cotyledons on calls formation and plant regeneration of stevia(Stevia rebaudiana Bertoni). The frequency of cellus formation was higher in the young leaf-explants then the older ones, and in the seeds then the hypocotyls and cotyledons on MS medium with 1mg/L 2,4-D. After transfer of seed-derived stevia callus producing embryogenic callus on plant-regeneration medium, the frequency of plant regeneration from callus was 23.8% in MS medium with 1mg/L NAA and 3mg/L kinetin.

• The Plant Pathology Journal
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• v.17 no.6
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• pp.364.5-364
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• 2001

• Journal of Ginseng Research
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• v.22 no.4
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• pp.299-303
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• 1998

To know possibility of raw ginseng production for white- and red-ginseng by direct-sowing culture, seeds were directly sowed or seedlings were transplanted at soil condition of sandy loam in ginseng field. After cultivation, the characters of 5-year-old ginseng were investigated. Number of survived plants was increased with increase the seeding quantity in direct-sowing culture, the survived plants in direct-sowing culture was higher than that of transplanting one. Rate of the numbers of survived plants to numbers of seeds sowed in plots of 134 or 90 seeds sowed per tan, 180 x 90cm area, inspire of high number of survived plant was high compared to that of transplanting culture. Occurrence rate of rusty root of ginseng in direct-sowing culture was low significantly compared to that of transplanting culture. Root yield showed in the order of 134, 268, 90 seeds sowed, the values of yield in direct-sowing culture were high obviously compared to that of transplanting one. Individual root weight was increased with decrease the seeding quantity, however, the root weight in plot of 90 seeds sowed showed almost equal the weight in transplanting culture. The number of usable raw ginseng for white- and red-ginseng was increased with decrease of the seeding quantity; the numbers were higher than that of transplanting culture remarkably. We concluded that optimum seedling rate in direct-sowing culture of ginseng was 90 to 134 seeds per tan considering the yield per area and production rate of large root.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.76-76
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• 2019

The purpose of this study was to evaluate the antioxidant and whitening action of Fagopyrum esculentum and F. tataricum buckwheat seeds depending on their germination time. In a previous study, we reported significant changes in sprout yield and phytochemical content in ethanol extracts from F. esculentum and F. tataricum seeds with increase in germination times. DPPH radical scavenging activities of F. tataricum increased with increasing germination time, whereas that of F. esculentum decreased. Next, we investigated anti-melanogenic activities of these species by estimation of melanin content and tyrosinase activity. Inhibition of melanin production in ${\alpha}-MSH$ (${\alpha}$-melanocyte stimulating hormone)-induced B16F10 cells by extracts from these seeds were analyzed. Among the two, F. tataricum extracts were characterized by higher inhibitory activity against melanin production. In addition, when B16F10 cells were incubated with L-DOPA for detection of in situ tyrosinase activity, F. tataricum and F. esculentum extracts were observed to reduce melanin production in cells. Taken together, these results indicate that extracts from buckwheat seeds could influence cellular processes via modulation of tyrosinase activity. Hence, buckwheat seeds could be utilized as whitening agents in the cosmetic industries and as therapeutics for hyperpigmentation disorders in a clinical setting.

• Journal of Plant Biotechnology
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• v.7 no.4
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• pp.219-223
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• 2005

We investigated chemical-wounding effect on Agrobacterium-mediated Chinese cabbage transformation via vacuum infiltration. Pre-germinated or germinating Chinese cabbage seeds were infiltrated with Agrobacterium tumefaciens LBA4404 cells carrying either GUS gene (pBI121) or hepatitis B virus surface antigen DNA (pBIHBsAg). Prior to agroinfiltration process, the seeds were soaked in sodium hydrosulfite (SHS) solution or just in sterile water as a control. Comparative transformation efficiency was determined by both of histochemistry and ELISA. We could demonstrate that SHS solution treatment especially to 1-day or 2-days old germinating seeds efficiently improved transformation process, and therefore, transient expression level. This strongly indicated that Agrobacterium infection could be facilitated indeed by SHS-causing wounds on Chinese cabbage seeds.

• Plant Biotechnology Reports
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• v.3 no.1
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• pp.1-56
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• 2009

Orchid seeds are nearly microscopic in size. Because of that, many fanciful theories were proposed for the origin of orchids. Almost 400 years separate the time when orchid seeds were seen for the first time and the development of a practical asymbiotic method for their germination. The seeds were first observed and drawn during the sixteenth century. Seedlings were first described and illustrated in 1804. The association between orchid and fungi was observed as early as 1824, while the requirement for mycorrhiza for seed germination was established in 1899. An asymbiotic method for orchid seed germination was developed in 1921. After Knudson's media B and C were formulated, orchids growing and hybridization became widespread. Hybrids which early growers may not have even imagined became possible.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.44-49
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• 2014

In this study, we investigated the effects of sodium hypochlorite (NaOCl) and red or blue light-emitting diode (LED) light on embryo swelling and germination of Oreorchis patens (Lindl.) Lindl. A method for determining the swelling and protocorm formation in O. patens seeds through in vitro examination of immature seeds was established. NaOCl treatment of immature seeds was found to significantly enhance the extent of embryo swelling and protocorm formation in immature zygote embryos compared to those in the untreated controls. Additionally, the effects of white fluorescent light, and red and blue LED lights on embryo swelling and protocorm formation in in vitro cultured seeds were examined and compared to the conditions with or without NaOCl treatment. The most suitable light for embryo swelling and protocorm formation was the red LED light.