• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.2
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• pp.446-451
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• 2005

This experiment was designed to investigate the effect of Mominis Placenta herbal acupuncture solution(HP-HAS) on immune cells and cytokines in murine asthma model. In vivo C57BL/6 mice were sensitized and challenged with OVA for 12 weeks. The experimental group was treated with Hominis Placenta herbal acupuncture solution(HP-HAS) at P'yesu(BL13) for the later 8 weeks(3 times a week) and analyzed by ELISA, flow cytometer. The results were obtained as follows Eosinophils in BALF(bronchoalveolar lavage fluid) of HP-HAS group decreased significantly compared with that of control group. IL-4, IL-5, IL-13, IgE in BALF of HP-HAS group decreased significantly compared with that of control group. Number of $CD3e^-/CCR3^+$, $CD69^+/CD3e^+$, $CD11b^+/Gr-1^+$ cells in the HP-HAS group decreased compared with that of control group.

• Korean Journal of Acupuncture
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• v.26 no.2
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• pp.53-60
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• 2009

Objectives: To investigate the effects of Hominis placenta pharmacopuncture on the blood picture and antioxidative activity in rats. Methods: Sprague-Dawley rats were divided into 3 groups; normal control (n=5), pharmacopuncture at CV12 (CV12 group, n=5), and pharmacopuncture at ST36 (ST36 group, n=5) once every other day for 4 weeks. Blood cell counting was performed and liver superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were analyzed. Results: Values of red blood cell and plasma cell volume were significantly higher in the ST36 group than the normal control. Values of hematocrit, total protein, and albumin were not significantly different among groups. White blood cell count and the percentage of neutrophils, lymphocytes, and eosinophils were not significantly different among groups. However, monocytes and basophils were significantly increased in the ST36, and CV12 groups, respectively. SOD and CAT in the CV12 and ST36 groups were significantly activated than in the normal control group, while the activity of GSH-Px showed no significant difference among groups. Conclusions: Based on the findings of this study, Hominis placenta pharmacopuncture may have positive impact on antioxidative capacity, thus activate various functions of the body.

• Journal of Environmental Health Sciences
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• v.20 no.3
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• pp.39-48
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• 1994

The purpose of this study was to determine the mercury accumulated at maternal and fetal organs, and compare its levels between maternal and fetal organs on day 20 of gestation, in pregnant Fisher-344 rats which given orally methylmercuric chloride on day 7 of gestation. Pregnant rats were divided four groups by dose: control group, and methylmercuric chloride treatment groups of 10, 20 and 30 mg/kg, respectively. The results obtained are as follows: I The mercury concentrations in maternal organs were the highest in kidney, and followed by blood, spleen, liver and brain. 2. The slopes of regression equation among mercury dose levels in maternal organs were as follows: Kidney 3.62 (r$^2$=0.943), Blood 2.75 (r$^2$=0.941), Spleen 2.49 (r$^2$=0.990), Liver 1.13 (r$^2$= 0.949), Brain 0.33 (r$^2$=0.984). 3. The mercury concentrations in fetal organs and placenta were the highest in liver, and followed by kidney, placenta and brain. 4. The slopes of regression equation among mercury dose levels in fetal organs and placenta were as follows: Liver 1.79 (r$^2$= 0.968), Kidney 0.79 (r$^2$= 0.976), Placenta 0.68 (r$^2$= 0.920), Brain 0.52 (r$^2$= 0.978), All Body 0.58 (r$^2$= 0.941). 5. As to the mercury levels in kidney, dams were 4.8~14.9 times higher than fetus. But as to the mercury levels in liver and brain, fetus were 1.6~2.5 and 1.5~1.9 times higher than dams. In conclusion, the mercury which exposured to pregnant rats can easily pass through the placenta and accumulated in fetus, especially higher in fetal liver and brain.

• Journal of Pharmacopuncture
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• v.8 no.3
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• pp.87-97
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• 2005

Objectives : This study has been designed and performed to identify the effect on Bell's palsy according to the injection of herbal medicine induced from the Hominis Placenta. Methods : We measured the facial palsy changes of the patients who were admitted for Bell's palsy in the Oriental Medical hospital of Dongeui medical center from 03-01-2004 to 07-31-2004. Bell's palsy patients were divided into two groups. One group(A group) was injected with Hominis Placenta herbal acupuncture. The other group(B group) was injected with normal saline. Then effects of these treatment was evaluated by Yanagihara's unweighted grading system. Results : A group was marked more higher than B group in treatment outcome. we discovered that it is significant differences between two groups after 4 week. Conclusions : These results provided that A group is more effective than B group. For clearly proving the effect of Hominis Placenta herbal acupuncture on Bell's palsy, it is need more sample's number and more treatment's duration.

• Journal of Pharmacopuncture
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• v.11 no.3
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• pp.123-131
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• 2008

Objective: This study was designed to evaluate the effect of Hominis placenta Pharmacopuncture treatment on Dysmenorrhea of Women. Methods: 49 subjects who were suffering from dysmenorrhea volunteered to answer the MMP(Measure of Menstrual Pain) and MSSL(Menstrual Symptom Severity List) questionnaire. They were divided into two groups, a Hominis placenta Pharmacopuncture treatment group(Experiment al group, n=25) and a Normal Saline(N/S) treatment group(Control group, n=24). The two groups were injected on the $CV_4,\;S_{36},\;Sp_9$ and $Sp_6$ acupuncture point. They were treated totally five times depending on the individual menstruation cycles. The scores of MMP and MSSL were measured overall three times before and after the menstruation cycle. The collected data were analyzed as paired t-test, independent t-test using SPSS 12.0 WIN Program. Results: As a result of the evaluation by MMP and MSSL, a significant improvement on dysmenorrhea was made in the two groups(p<0.05), and both scores of Experiment group were decreased more than Control group. But there was no significant difference between the two groups. Conclusions: The Hominis placenta Pharmacopuncture treatment and the Normal Saline treatment were effective in decreasing the symptom of Dysmenorrhea.

• Biomedical Science Letters
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• v.18 no.2
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• pp.169-174
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• 2012

The placenta is an extraembryonic tissue that is formed between mother and fetus and mediates delivery of nutrients and oxygen from the mother to the fetus. Because of its essential role in sustaining the growth of the fetus during gestation, defects in its development and function frequently result in fetal growth retardation or intrauterine death, depending on its severity. Vertebrate Hox genes are well known transcription factors that are essential for the proper organization of the body plan during embryogenesis. However, certain Hox genes have been known to be expressed in placenta, implying that Hox genes not only play a crucial role during embryonic patterning but also play an important role in placental development. So far, there has been no report that shows the expression pattern of the whole Hox genes during placentation. In this study, therefore, we investigated the Hox gene expression pattern in mouse placenta, from day 10.5 to 18.5 of gestation using real-time RT-PCR method. In general, the 5' posterior Hox genes were expressed more in the developing placenta compared to the 3' Hox genes. Statistical analysis revealed that the expression of 15 Hox genes (Hoxa9, -a11, -a13/ -b8, -b9/ -c6, -c9, -c13/ -d1, -d3, -d8, -d9, -d10, -d11, -d12) were significantly changed in the course of gestation. The majority of these genes showed highest expression at gestational day 10.5, suggesting their possible role in the early stage during placental development.

• Biomolecules & Therapeutics
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• v.25 no.5
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• pp.528-534
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• 2017

Placenta is a special organ that contains many nutrients such as growth factors, minerals, and bioactive peptides. Dipeptides of glycine and leucine are major components of porcine placenta extracts (PPE) that has been used as an alternative of human placenta extracts. In this study, we investigated whether major peptides of PPE, Glycyl-L-Leucine (Gly-Leu), L-Leucyl-Glycine (Leu-Gly), and L-Leucyl-L-Leucine (Leu-Leu), affect skin hydration and elasticity in vitro and in vivo. We found that Gly-Leu and Leu-Gly dipeptides induced the expression of transglutaminase 1 in normal human epidermal keratinocytes (NHEKs) whereas Leu-Leu dipeptides did not. Treatment with Gly-Leu or Leu-Gly significantly increased hyaluronan (HA) synthesis in NHEKs and the upregulation of hyaluronan synthase 2 (HAS2) mRNA level was confirmed. In addition, elastase activity was inhibited in NHEKs treated with Gly-Leu or Leu-Gly dipeptides. Oral administration of Gly-Leu or Leu-Gly dipeptides increased skin hydration and elasticity in UVB-irradiated hairless mice. The significant upregulation of HA in UVB-irradiated hairless mice was observed in response to oral administration of Gly-Leu or Leu-Gly. These results suggest that the major dipeptides of porcine placenta, Gly-Leu and Leu-Gly, are potentially active ingredients for skin moisturization formulations.

• Korean Journal of Acupuncture
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• v.30 no.4
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• pp.319-328
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• 2013

Objectives : The purpose of this research was to compare the effect of general acupuncture therapy to Hominis placenta Pharmacopuncture therapy on pain and discomfort of severe dyspepsia in upper abdomen. Methods : We investigated 60 cases of patients with severe dyspepsia, who had been treated at Dept. of Internal Medicine, College of Oriental Medicine Dongeui University from May 2011 to September 2012. We divided patients into two groups : Group I was treated by general acupuncture(ACU, n=30) and Group II was treated by Hominis placenta Pharmacopuncture therapy(PHA, n=30). The symptoms and quality of life were measured with the Nepean dyspepsia index-Korean version(NDI-K) at baseline and 2 weeks. The responsiveness of the NDI-K was evaluated by correlation with symptom scores. Results : In ACU group, the 13 symptom scores were significantly decreased after treatment(p<0.05). In PHA group, the 9 symptom scores were significantly decreased after treatment(p<0.05). In ACU group, the 3 Quality of life(QOL) scores were significantly increased after treatment(p<0.01). In PHA group, the 3 QOL scores were significantly increased after treatment(p<0.05). Conclusions : General acupuncture therapy and Hominis placenta Pharmacopuncture therapy improves the symptoms and the quality of life in patients with severe dyspepsia. But there were no significant differences statistically between the two groups.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.9
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• pp.4405-4408
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• 2016

Background: To investigate the characteristics of allelic distribution of IGF2 and H19 gene polymorphisms in molar tissues compared to normal placentas. Materials and Methods: Forty-nine specimens of molar tissues as well as 100 control normal placental tissues, delivered on the same days, were collected. Polymerase chain reaction (PCR) with restriction fragment length polymorphism (RFLP) on 2% agarose gel electrophoresis was conducted to determine the allelic distribution. The ApaI polymorphism within exon 9 of IGF2 and the RsaI polymorphism within exon 5 of H19 were employed to identify the allelic distribution of the IGF2 and H19 genes, respectively. Then the data for these genes in the molar and normal placenta tissues were compared. Results: The allelic distribution of IGF2 genes found in molar tissue were 21 (42.9%) aa (undigested), 10 (20.4%) ab (heterozygous) and 18 (36.7%) bb (digested), while in normal placenta tissue the values were 22 (22%) aa, 51 (51%) ab, and 27 (27%) bb. The allelic distribution of H19 in molar tissues was 8 (16.2%) aa (undigested), 8 (16.3%) ab (heterozygous) and 33 (67.4%) bb (digested) and in normal placental tissue was 16 (16%) aa, 36 (36%) ab and 48 (48%) bb in normal placenta tissue. These results were significantly different with P values of 0.001 and 0.037 for the allelic distribution of IGF2 and H19, respectively. Conclusions: Molar tissues showed significant differences of allelic distribution of IGF2 and H19 from normal placenta tissues.

• Journal of Physiology & Pathology in Korean Medicine
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• v.32 no.2
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• pp.141-147
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• 2018

The purpose of this research is to show the effectiveness of spinal stenosis treated by Hominis Placenta Megadose pharmacopuncture combined with Korean medicine treatment. We used Hominis Placenta Megadose pharmacopuncture treatment on patients who received treatment at Bucheon Jaseng Korean medicine hospital from March, 2016 to October, 2016. Also we checked Numeric Rating Scale(NRS), Oswestry low back pain Disability Index(ODI) to follow the scale showing the improvement of the symptoms of the patients. All 4 patients showed the improvement of NRS, ODI scales. This research suggests Hominis Placenta Megadose pharmacopuncture combined with Korean medicine treatment can help treat spinal stenosis.