• The Korean Journal of Ecology
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• 제14권2호
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• pp.149-157
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• 1991

To find out allelopathic potential of naturally occurring substances emitted form pinus korairnsis, water extracts and volatile sudstances of the tree materials werw provided and used for tests such as seed germination and seedling growth test. In general, the more was the concentration of the extracts and essential oils, the worse was growth effects of the selected species. The germination an the growth were inversely proportional to the concentration of the extracts and the essential oils of p. koraiensis. in other words, the chemical substances had the biological toxic activity. gc and gc/ms methods were employed for analysis and identification of phytotoxic substances from the tree leaves. Sixteen chemical compounds were identified from water extracts and nineteen from essential oils of p. koraiensis leaves. Through the experiment on germination and seedling growth test, the chemical substances naturally occurring from p. koraiensis seemed to be responsible for the allelopathic potential in this study.

• 한국균학회지
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• 제43권4호
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• pp.272-276
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• 2015

경기도와 강원도에 위치한 화악산, 명지산 그리고 설악산에서 침엽수를 채집하여 균 분리를 수행하였다. 특히, 눈측백과 분비나무의 내생균에 대한 연구가 최초로 수행되었으며, 본 연구를 통해 이들로부터 분리한 균에 대한 형태적, 유전적 동정을 수행하였다. 그 결과 Cryptosporiopsis actinidiae, Pestalotiopsis mangiferae, Pyrenochaeta cava가 확인되었으며, 이에 대해 최초로 국내에서 눈측백과 분비나무에 분포함을 보고하였다.

• Journal of Applied Biological Chemistry
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• 제60권1호
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• pp.73-78
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• 2017

본 연구는 잣나무(Pinus koraiensis) 잎을 열풍건조, 음지건조, 동결건조하여 물과 80 % ethanol을 추출용매로 사용하여 추출물이 미용식품의 소재로서의 기능성을 증명하고자 실시하였다. Total phenolic compounds는 WE에서는 17.93 mg/g로 음지건조가 가장 높았고, EE에서도 20.63 mg/g로 음지건조 추출물이 가장 높은 용출량을 나타내었다. DPPH, ABTS radical 전자공여능 측정 결과, WE는 열풍건조에서 90.45, 99 %, EE에서는 음지건조가 96.2, 99 %의 높은 활성을 나타내었다. PF 측정 결과, 음지건조 잣나무 잎 추출물에서 WE는 9.63 PF, EE에서는 10.48 PF의 결과를 나타내었고, TBARs 측정 결과, 동결건조 잣나무 잎의 WE에서 82.07 %와 음지건조 잣나무 잎의 EE에서는 89.39 %의 가장 높은 높은 활성을 나타내었다. 주름개선 효과를 측정 결과, elastase 저해 활성은 동결건조 잣나무 잎 EE에서 71.46 %, collagenase 저해 활성은 음지건조 잣나무 잎의 EE에서 97.48 %로 가장 높은 저해 활성을 나타내었다. Melanin 생합성을 억제할 수 있는 tyrosinase 저해 활성은 음지건조 잣나무 잎의 EE 저해 활성이 63.03 %로 가장 높았다. 이러한 결과로 폐자원으로 여겨지는 잣나무 잎을 음지건조 하였을 때 항산화능뿐만 아니라, 주름 및 미백 활성이 특히 높은 것으로 보아 향후 잣나무 잎이 기능성 미용 소재 산업에 활용가치를 기대할 수 있었다.

• 농업과학연구
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• 제38권1호
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• pp.11-16
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• 2011

In this study, we report the generation and analysis of a total of 1,211 expressed sequence tags (ESTs) from Pinus koraiensis. A cDNA library was generated from the young leaf tissue and a total of 1,211 cDNA were partially sequenced. EST and unigene sequence quality were determined by computational filtering, manual review, and BLAST analyses. In all, 857 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length 50 nucleotides. A total of 411 unigene, consisting of 89 contigs and 322 singletons, was identified after assembling. Also, we identified 77 new microsatellite-containing sequences from the unigenes and classified the structure according to their repeat unit. According to homology search with BLASTX against the NCBI database, 63.1% of ESTs were homologous with known function and 22.2% of ESTs were matched with putative or unknown function. The remaining 14.6% of ESTs showed no significant similarity to any protein sequences found in the public database. Gene ontology (GO) classification showed that the most abundant GO terms were transport, nucleotide binding, plastid, in terms biological process, molecular function and cellular component, respectively. The sequence data will be used to characterize potential roles of new genes in Pinus and provided for the useful tools as a genetic resource.

• KSBB Journal
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• 제28권6호
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• pp.380-386
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• 2013

This study was to investigate the chemical composition and biological activities of Pinus Koraiensis seed. The oil, moisture, ash, crude protein, and carbohydrate contents of P. Koraiensis seed were 58.21, 7.84, 1.56, 14.26, and 18.13%, respectively. The ratios of essential amino acid and nonessential amino acid against total amino acids were 36.6 and 60.3%, respectively. The saponification value of seed oil was 166.8 mg KOH/g oil. Among various fatty acids, the linoleic acid content was the highest, 35.5%, which was approximately 72.6% of polyunsaturated fatty acid. The ethylacetate extract of P. Koraiensis seed had the highest DPPH radical scavenging activity (62.8%) at 7.0 mg/mL, followed by hexane extract, methanol extract, and hot water extract. The maximum nitrite scavenging activity was obtained 59.3% at pH 1.2. The total phenolic concentration of ethylacetate extract was 98.7 mg/g, approximately 4.8 folds higher than that of the hot water extract. The maximum inhibition activities of elastase using ethylacetate extract and collagenase using hexane extract were 58.8 and 40.7%, respectively. These results indicate that P. koraiensis seed extract could be applied to present the possibilities of industrial applications for the developments of cosmetics.

• Mycobiology
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• 제50권5호
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• pp.317-325
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• 2022

Endophytic fungi occupy various ecological niches, which reinforces their diversity. As few studies have investigated the endophytic fungi of alpine conifers, we focused on four species of alpine conifers in this study-Abies nephrolepis, Pinus pumila, Taxus cuspidata var. nana, and Thuja koraiensis-and examined them for endophytic fungi. A total of 108 endophytic fungi were isolated. There were four taxa in A. nephrolepis, 12 in P. pumila, 18 in T. cuspidata var. nana, and 17 in T. koraiensis; these were divided into five classes: Agaricomycetes (3.2%), Dothideomycetes (29.0%), Leotiomycetes (15.0%), Sordariomycetes (41.9%), and Orbiliomycetes (1.6%). The most prevalent fungi were Sydowia polyspora (22.7%) and Xylariaceae sp. (22.7%) in P. pumila, Phomopsis juglandina (16.1%) in T. cuspidate var. nana, and Thuja-endophytes sp. 1 (70.0%) in T. koraiensis. However, there was no dominant species growing in A. nephrolepis. Some host plants were analyzed using next-generation sequencing. We obtained 4618 reads for A. nephrolepis and 2268 reads for T. koraiensis. At the genus level, the top three endophytic fungi were Ophiostomataceae_uc (64.6%), Nectriaceae_uc (15.5%), and unclassified organism (18.0%) in A. nephrolepis and Nectriaceae_uc (41.9%), Ophiostomataceae_uc (41.8%), and Magnaporthaceae_uc (9.2%) in T. koraiensis. Our results show that there are different communities of endophytic fungi among different host plants, even if the host plants are in the same region. Such ecological niches are important in terms of the ecological restoration of alpine conifers.

• 환경위생공학
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• 제5권1호
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• pp.28-33
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• 1990

춘천의 잣나무림과 관악산의 소나무림에 있어서 낙엽의 무기화를 연구하였다. N, P, K, Ca 및 Na의 무기화 상수를 보면 잣나무림에서는 각각 0.064, 0.068, 0.040, 0.047, 0.417, 0.058이었으며, 소나무림에서는 0.049, 0.049, 0.346, 0.058이었다. 무기물이 무기화되는 순서는 잣나무림에서는 Ca>P>N>Na>K였으며 소나무림에서는 Ca>Na>N=P>K로 나타났다. 일반적으로 양이온의 무기화는 음이온의 무기화보다 빨랐다.

• The Plant Pathology Journal
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• 제16권1호
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• pp.52-54
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• 2000

Pitch canker of Pinus rigida, caused by Fusarium subglutinans f. sp. pini, was first noticed in Inchon, Korea, and is now being spread into other plantations of pines. Typical symptoms re resin flows from canders on the shoots, branches, and trunks, and resin-soaking of the sap wood under the bark. Pathogenic fungi were isolated from the infected shoots, branches, and sees on Fusarium-selective medium. Mycelial growth and microscopic characteristics were examined. Pathogenicity test was carried out by inoculating four common species of pines (P. rigida, P. densiflora, P. thunbergii, and P. koraiensis) in Korea. P. rigida and P. thunbergii showed symptoms identical to those of naturally infected trees, while P. densiflora and P. koraiensis remained free symptomless.

• 한국환경복원기술학회지
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• 제11권1호
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• pp.85-95
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• 2008

This study was carried out to analyze Sungkyunkwan University Forest vegetation at Pocheon. Employing the releve method of Braun-Blanquet, 20 plots were sampled. The communities were classified into Larix leptolepis community, Quercus mongolica community, Pinus koraiensis community and Pinus densiflora community. Average coverage rate was 91% in tree layer, 39% in subtree layer, 44% in shrub layer, 43% in herb layer, respectively. The importance values were 61.79% in Larix leptolepis, 52.08% in Quercus mongolica, 44.6% in Pinus koraiensis, 30.35% in Pinus densiflora, 17.78% in Styrax obassia, 10.9% in Fraxinus rhynchophyllia. DBH range of most Quercus mongolica was between 6cm and 15cm. Therefore, Quercus mongolica will be dominant species in the study area for several more decades.

• Journal of the Korean Wood Science and Technology
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• 제12권4호
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• pp.36-46
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• 1984

한국식목질과 포틀랜드 시멘트(Type I)로 건축재개발가능성을 구명하기 위하여 소나무, 리기다 소나무, 잣나무, 일본잎갈나무, 현사시나무 목질과 왕겨 및 볏짚과 포틀랜드 시멘트 그리고 물과 혼합하여 24시간동알 수화열을 측정하고 수화억제지수를 산출한 바 리기다 소나무와 소나무가 한정된 조건하에서 목질-시멘트 복합체에 적절한 수종이며, 열수추출 수행하면 경화억제지수가 개선되어 왕겨, 잣나무, 일본잎갈나무는 한정된 조건하에서 적절한 수종이고 열수추출과 경화촉진제와 염화칼슘이나 염화마그네슘을 경울하면 현사시나무 일본 잎 갈나무도 리기다 소나무와 아울러 우수한 수종으로 판명되었다.