• Mycobiology
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• v.41 no.2
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• pp.77-81
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• 2013

This study aimed to investigate differences in ectomycorrhizal (ECM) fungal communities between disturbed sites and undisturbed old forest sites. ECM root tips of Pinus densiflora were collected from 4 sites disturbed by human activities and 3 undisturbed old forest sites adjacent to the disturbed sites. Results in this study showed that the number of ECM root tips, species diversity, and number of species were significantly higher in the disturbed sites than in the undisturbed sites, suggesting that the ECM fungal community structure was affected by the degree of disturbance.

• The Korean Journal of Mycology
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• v.48 no.2
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• pp.117-124
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• 2020

In this study, we isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Mt. Gyehwasan in Korea. We identified the isolated fungal strains based on their morphological characteristics and phylogenetic analysis using DNA sequences of the internal transcribed spacer, small subunit rDNA, large subunit rDNA, and β-tubulin region. In the process of this study, we confirmed two endophytic fungi that have not been recorded previously in Korea, Lophiostoma macrostomum and Pestalotiopsis lespedezae. In this report, we described the morphological characteristics of these fungal strains and the results of their molecular analysis.

• 보존과학연구
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• s.28
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• pp.59-73
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• 2007

Wooden artifacts are biologically damaged by animals, insects, and micro-organisms. We focused on the evaluation of the antifungal and insecticidal activities of tree extracts to control deterioration of wooden cultural properties. 12 kinds of methanol extracts from 9 species of tree were investigated for their biocidal activities against two micro-organisms and two spices of insect. Most tree extracts showed fungicidal activities to Tyromyces palustris and Trametes versicolor. Among 12 different tree extracts, the strongest antifungal activity against T.palustris and T.versicolor was observed from the extracts of Pinus densiflora stem-bark extract and Pinus koraiensis leaf extracts. However, in the insecticidal activity test, Pinus densiflora stem-bark extracts against termite was relatively effective. Chemical compositions of methanol extracts were analyzed by gas chromatography/mass spectrometry. As a result, it was confirmed that development of biocide based on the natural extracts can be expected to conservate of wooden cultural properties.

• Journal of Plant Biology
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• v.36 no.1
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• pp.29-34
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• 1993

Observation was made on early ontogeny of vascular cambium in the cotyledonary node of Pinus koraiensis seedling in order to clarify the cambial ontogeny of the node which was hardly elongated. In transverse view, the derivatives by the early periclinal divisions at the outer region of the procambial strands differentiated to protophloem with establishing a certain degree of radial seriations. Later, some cells in the central region of the procambial strands begin divide periclinally, eventually the cells, differentiate gradually to the vascular cambium. In tangential view, early procambium is consisted of homogeneous short cells with transverse end walls which are tansformed into the long cells and short cells gradually. The long cells continue intrusive growth and the short cells repeat transverse division. Finally, the long and short cells differentiated to cambial fusiform initials and ray initials respectively. In tangential section, the differentiation pattern of cambial initials resembles that of the stem of Ginkgo biloba. But in transverse view, the characteristics in the origin of vascular cambium of the cotyledonary node resembles that of root. The vascular cambium in cotyledonary node is completed after eleven prophylls were formed.

• Korean Journal of Plant Resources
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• v.12 no.1
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• pp.10-19
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• 1999

The water status of Pinus densiflora and P. koraiensis was observed after the treatment of simulated acid rain for every month August 1997 to March 1998. Both of the species showed increase of dry weight, water saturation deficit and epidermis transpiration after treatment of simulated acid rain (pH 3.5) for first three months. On the contrary, water content was decreased in both the species after three month of simulated acid rain treatment. The effect of simulated acid rain, however, became weaker after that and no big difference was observed after seven month of treatment. Water potential of the leaf of both species became lower than that of the control(pH 5.6) after seven month treatment of simulated acid rain. The cuticle layers of the epidermis and florins ring were damaged seriously and the size of the gap between stomata became bigger after the treatment of simulated acid rain.

• The Korean Journal of Ecology
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• v.15 no.3
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• pp.231-246
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• 1992

In order to estimate effects of air pollution for pinus thunbergii forests arounding onsan industrial complex, structure of forest vegrtation was surveyed. In the survey of forest vegetation, pinus densiflora have been died or being withered, and importance value(I.V) of rhododendron mucromulatum and lespedeza spp. were low at the vicinity of industrial complex. Especially lespedeza maximowiczii and l. maritima were not entirely appeared at the vicinity of industrial complex. I.V. of quercus serrata, robinia pseudoacacia and alnus firma were preferably high at the vicinity of industrial complex, and those of smilax china, rubus crataegifolius and rhus trichocarpa were similar at every site and it could be guessed that those had a tolerance. Number of species had a difference over 10 species at the vicinity of industrial complex as compared with those of remote sites, and species diversity showed highly at the farther sites of industrial complex, and also at the low layer, number of species, species diversity and evennes were low at the vicinity of industrialcomplex and species weakened to air pollution were disappearing. It was possible that forest vegetation would be changed from pinus spp. to community of a. firma and r. pseudoacacia.

• The Korean Journal of Ecology
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• v.8 no.4
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• pp.197-200
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• 1985

The relations of crown width and DBH for Pinus densiflora, Pinus rigida, Pinus koraiensis, and Ginkgo biloba were accomplished to estimate the level of crown competition. Measurements of the relations revealed that crown width and DBH were highly correlated for the same species. Also it seems that these relations are independent of age and site quality. The results of regression analysis were as follow: P. densiflora, Y=0.3477X+0.3828 r=0.95 p. rigida, Y=0.3537X+0.1645 r=0.95 P. koraiensis, Y=0.2895X+0.6310 r=0.92 G. biloba, Y=0.4360X+0.0995 r=0.90 The significant differences between G. biloba and pine species seems due to their structural differences of crown formation according to tree species. As results of computing Maximum Crown Area and Crown Competition Factor as indices of crown competition, they indicated that P. densiflora would grow better under the natural conditions.

• Natural Product Sciences
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• v.16 no.1
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• pp.10-14
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• 2010

Phytochemical examination of the barks of Pinus densiflora Siebold et Zuccarini has led to the isolation of one phenylpropanoid, one lignan, one flavonoid, one flavan 3-ol and two procyanidins : 4-O-$\beta$-D-glucopyranosyl-p-coumaric acid (1), 2,3-dihydro-2-(4-methoxy)-7-hydroxy-3-hydroxymethyl-5-(3-hydroxy propyl)-benzofuran 3-O-$\alpha$-D-glucopyranoside (2), taxifolin 3'-O-$\beta$-D-glucopyranoside (3), (+)-catechin (4), procyanidin B1 (5) and epicatechin-($4{\beta}$-8)-catechin-($4{\alpha}$-8)-catechin (6). Among them, Compound 4, 5 and 6 showed potent anti-oxidative activities and these anti-oxidative activities were significantly different compared with ascorbic acid as positive control.

• Journal of Plant Biology
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• v.27 no.2
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• pp.51-60
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• 1984

Jisimdo is an island where evergreen broadleaved forests are well preserved. Soil environments and forest structures of Jisimdo were investigated, and an actual vegetation map and profile diagrams were drawn out. The natural vegetation of Jisimdo was divided into two stand units, one was evergreen broadleaved forest and the other was Pinus thunbergii forest. 26 species were identified as evergreen broadoeaved trees, and among them, Camellia japonica was the dominont of the tree layer of evergreen broadleaved forest. Profile diagram shows that Camellia japonica, with average height of 7∼8m, formed lower tree layer, and laurels like Cinnamomum japonicum, Machilus thunbergii, and Neolitsea sericea formed upper tree layer. In Pinus thunbergii forest, plants of shrub and herb layers were abundant because of much light penetrated into the forest floor, and these layers were largely composed of evergreen broadleaved trees. This fact shows the possibility of succession from Pinus thunbergii forest into evergreen broadleaved forest. Jisimdo is geographically adjacent to Jangseungpo and Okpo, and this increases the economic value of Jisimdo as a place of public resort. It will be necessary from being destroyed by land development and human interferences.

• The Korean Journal of Mycology
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• v.46 no.4
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• pp.437-446
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• 2018

We investigated the biodiversity of endophytic fungi in Pinus densiflora and Junipreus rigida from Mt. Baekryeonsan and Mt. Johangsan in Korea. In total, 59 isolates were discovered from 26 host plants and identified using sequences from the rDNA internal transcribed spacer (ITS) region. Twenty taxa of endophytic fungi were identified in this study; 62% of isolates belonged to Leotiomycetes, 34% belonged to Sordariomyetes, and 4% belonged to Agaricomycetes. Results confirmed three previously unreported endophytic fungi in Korea: Phanerochaete concrescens, Creosphaeria sassafras, and Scopuloides rimosa. Community analysis showed that the fungal diversity patterns of the samples from Mt Baekryeonsan and Mt. Johangsan are similar to those from previous studies and varied depending upon host and locality.