• The Plant Pathology Journal
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• v.29 no.3
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• pp.344-349
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• 2013

Intraspecific variation in morphometry of pine wood nematodes Bursaphelenchus xylophilus and B. mucronatus in relation to geographical locations in South Korea was investigated using morphometric characters (body length, a, b and c ratio, stylet length, and spicule length for a male nematode and V (%) value for a female nematode). B. xylophilus was isolated from Pinus thunbergii in Jinju (1998), Ulsan (2000), Yangsan (2000), Mokpo (2001) and Jeju (2004), and from P. densiflora in Gumi (2001). B. mucronatus was isolated from P. thunbergii in Jinju (1991) and from P. densiflora in Milyang (2001). The body length of male and female B. xylophilus had the highest coefficient of variability and showed significant differences among geographical locations. The V (%) value for female B. xylophilus showed the lowest coefficient of variability, changing little with geographical area and host plant. All morphometric characters in B. mucronatus except for stylet length and female body length showed no significant differences between locations or hosts, suggesting they may not be affected by geographical area or host plant.

• Molecules and Cells
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• v.20 no.2
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• pp.201-209
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• 2005

We have constructed an AFLP-based linkage map of Japanese red pine (Pinus densiflora Siebold et Zucc.) using haploid DNA samples of 96 megagametophytes from a single maternal tree, selection clone Kyungbuk 4. Twenty-eight primer pairs generated a total of 5,780 AFLP fragments. Five hundreds and thirteen fragments were verified as genetic markers with two alleles by their Mendelian segregation. At the linkage criteria LOD 4.0 and maximum recombination fraction 0.25(${\theta}$), a total of 152 markers constituted 25 framework maps for 19 major linkage groups. The maps spanned a total length of 2,341 cM with an average framework marker spacing of 18.4 cM. The estimated genome size was 2,662 cM. With an assumption of equal marker density, 82.2% of the estimated genome would be within 10 cM of one of the 230 linked markers, and 68.1% would be within 10 cM of one of the 152 framework markers. We evaluated map completeness in terms of LOD value, marker density, genome length, and map coverage. The resulting map will provide crucial information for future genomic studies of the Japanese red pine, in particular for QTL mapping of economically important breeding target traits.

• Food Science and Biotechnology
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• v.15 no.4
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• pp.582-588
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• 2006

The antioxidant and antimicrobial activity of the fermentation extract (PFE) and the 50 and 80% ethanol extracts (PE 50, PE 80) of Pinus densiflora pine needles were evaluated. Electron donating ability, superoxide dismutase (SOD) ability, and antimicrobial activity were observed in PFE; those abilities differed in PE 80 and PE 50, depending on the ethanol concentration used for the extraction. PFE had the highest electron donating ability with a value of 92.20%, while PE 80 and PE 50 had values of 74.66 and 53.47%, respectively. For SOD activity, PE 80 exhibited a slightly higher value of 31.11% compared to that of PFE and PE 50, which were 29.65 and 25.43%, respectively. PFE, PE 50, and PE 80 were all found to inhibit bacteria, and the effectiveness of this inhibition was strongly related to the type of extracts used. PFE showed good antimicrobial effects for all of the tested Gram-positive strains and for most of the tested Gram-negative strains. These results suggest that PFE has superior functionality compared to the ethanol extracts (PE 80, PE 50), in terms of antioxidant and antimicrobial activity. On the basis of these results, pine needle fermentation extracts can be used for industrial applications as a functional material.

• Korean Journal of Environment and Ecology
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• v.23 no.1
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• pp.56-65
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• 2009

In Korea, Red pine(Pinus densiflora) stands at a very important place, historically, culturally, and emotionally and it is one of the tree species that can produce domestic timber as forest resources economically. The growing space for Red pine forest in Korea is gradually reducing while the space for deciduous tree forests including oak series is gradually increasing. Thus, it is required that the research work should be done on renewal for pine forest retention and its related forest management practices. This research aims at developing stable, sustainable management forests and inducing nature renewal by grasping growth environment and succession process through the pattern of stand and structure analysis of the red pine(Pinus densiflora) for central region. The pine forests in central region were classified into four communities, such as Acer pseudo-sibolianum, Quercus acutissima, Lindera erythrocarpa, and Pinus densiflora and they are showing different characteristics by pattern according to their growing district. There appeared a lot of red pines in the upper layer, but there existed high weight of broad-leaved forest tree species, such as oak series in the middle layer and the appearance of the red pine was meager in the lower layer. Therefore, it appears that the red pine has a high influential ecological strength in terms of correlativity; however, if the red pine in tree layer is dead by aging in nature state with the lapse of time, it is considered that oak species, such as Quercus mongolica, Quercus variabilis, and Quercus serrata will be dominant below sub-tree layer. In addition, there appear differences in characteristics and growth pattern of the red pine forest depending on stand pattern, so it is considered that a differential application method should be employed in the pine forest management.

• The Korean Journal of Ecology
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• v.20 no.5
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• pp.315-321
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• 1997

Peroxidase activities and isozyme patte군 of the pine needles (Pinus densiflora) were examined and compared in the coastal regions of Anmyum-Do(Choongnam, Taean-Gun) and inland regions of Shinchang-Myun(Choongnam, Asan-City). The pine needle peroxidase from Anmyum-Do showed approximately three times higher specfic activity than Shinchang pine needle peroxidase. The pine needle extracts of Anmyun-Do and Shinchang contained three anionic isoperoxidases, named A1, A2 and A3, when subjected to starch gel electrophoresis at pH 7.0. Cjationic isoperoxidases could not be found in both extracts., However, there existed unique isoperoxidase An only from the extracts of Anmyun-Do pine needles under the salinary environment. Moreover, the specific activities of catalase and glucose-6-phosphate dehydrogenase from Anmyun-Do, known for the inducible enzymes under the stress condition, were about 1.8 times higher than those of Shinchang pine needles. However, the specific activities of other enzymes did not show great differences between the two regions. Considering the above results of the higher specific activity of peroxidase and the unique expression of isoperoxidase An, pine needle peroxidase might involve in the defence mechanism against the salinary stress of Anmyun-Do.

• The Korean Journal of Ecology
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• v.25 no.6
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• pp.389-393
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• 2002

Effects of environmental factors on in vitro pine pollen performance were investigated. Pinus densiflora and P. rigida pollen grains collected at Mt. Kwanak, Korea were used. Three environmental factors, such as pollen storage temperature, pollen culture temperature and nutrient condition in medium, were tested. To determine the storage temperature effects on pollen viability, pine pollen was stored at $-70^{\circ}C$, $-12^{\circ}C$, $4^{\circ}C$ and $22^{\circ}C$. Pollen viability was substantially extended at the storage temperatures of $-12^{\circ}C$ and $4^{\circ}C$ for more than 300 days. To elucidate the culture temperature effects on pine pollen germination and tube growth, pollen grains were cultured at the temperatures from $5^{\circ}C$ to $40^{\circ}C$ at $5^{\circ}C$intervals. The germination rate and tube growth were highest at $25^{\circ}C$ and decreased above $30^{\circ}C$. To investigate boron and sucrose effects on pollen tube growth, the pollen was cultured at different sucrose and boric acid concentrations. Germination rate was optimal in germination medium containing 3 or 5$\%$ sucrose with 0.01 $\%$ boric acid. These results indicate that the pine pollen can be stored for considerable length of time without noticeable loss of viability at storage temperature below or near $0^{\circ}C$. Optimal germination medium conditions were established for pine pollen. Therefore, pine pollen can be used for many biological and environmental monitoring researches.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.4
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• pp.568-573
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• 1998

Volatile compounds in Pinus densiflora were extracted with Likens-Nickerson apparatus for three hours, and were separated and identified by gas chromatography(GC) and mass selective detector(MSD). Twenty six compounds were isolated from Pinus densiflora, identified by GC-MSD and twelve compounds were confirmed by matching retention times of the pure comounds. The main valatile compounds were terpenoids such as limonene(36.2%), $\beta$-pinene(16.9%), $\beta$-myrcene(12.6%) and $\alpha$-pinene(10.9%), and the total amount of these main compounds was 367.9$\mu\textrm{g}$ per 1g of pine twigs.

• The Korean Journal of Ecology
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• v.24 no.6
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• pp.341-348
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• 2001

Population dynamics of the oldest relic stand of Pinus densiflora for. erecta which remains as a status of patch in Sokwang-Ri area, Uljin-Gun, was studied to provide the basic data for conservation of the oldest pine stand in Korea. The upper slope site and ridge site including total 12 plots mainly represented Pinus densiflora for. erecta and Quercus mongolica. These two tree species showed reverse J-shaped distribution. From this information, it was concluded that intensive interspecific competition between two species could be occurring. Age distribution of P. densiflora for. erecta on the sites above middle slope area was indicated bi-modal type or tri-modal type which was known as an age structure of climax forest. Therefore, it was estimated that Pinus densiflora for. erecta population could be continuously maintained in the each patch above middle slope area. In the valley sites, hardwood species such as Q. mongolica, Q. variabilis, Styrax obassia and Acer pseudosieboldianum occupied most part of the under crown-cover area and their seedlings and saplings were developing into the closed upper layer crown because the height of upper layer crown reached about 35 meters and such a high crown height was more suitable for light utilization by plants of under layer. The growth patterns were mainly fluctuated for the entire life time of the oldest tree caused by frequent disturbance. And the initial growth curves of the mature trees and suppressed juvenile trees showed different tendencies along the sites.

• Journal of the Korean Wood Science and Technology
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• v.27 no.4
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• pp.15-19
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• 1999

Three wood coffins of Chosun Dynasty period buried about 450 years ago were excavated in the sound condition in Andong area in the early 1998. The proprietors of wood coffins were grandparents, Mr. Myoung Jong Lee and Mrs. Mun, and their grandson, Mr. Eung Tae Lee, and the social standing of their family was known to belong to the nobility in those days by the clan genealogy. All the wood coffins investigated through light microscopy had same anatomical characteristics as follows: abrupt to somewhat abrupt tracheid transition from earlywood to latewood; normal longitudinal and horizontal resin canals with thin-walled epithelium; tylosoids in resin canals; bordered pits frequently in 1 row on radial walls of tracheids; 1 or 2 window-like pits per cross-field; uniseriate and fusiform rays; heterogeneous rays composed of body ray parenchyma cells and marginal ray tracheids or homgeneous rays composed of only ray tracheids; dentate ray tracheids; occasional trabeculae traversing tracheids in radial direction. Based on theses microscopic characteristics, all the wood coffins were identified to be Korean red pine (Pinus densiflora) or Korean black pine (Pinus thunbergii). Korean black pine growing naturally in coastal area might not be probable because the site of excavation, Andong area, was mountainous and inland area of Korea Thus, Korean red pine was thought to be the possible species for the wood coffins because of its natural distribution through the Korean Peninsula and the easy availability.

• Korean Journal of Agricultural and Forest Meteorology
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• v.22 no.2
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• pp.47-56
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• 2020

Decline of pine forests happens in Korea due to various disturbances such as insect pests, forest fires and extreme climate, which may further continue with ongoing climate change. For conserving and reestablishing pine forests, understanding climate-induced future shifts of pine tree distribution is a critical concern. This study predicts future geographical distribution of Pinus densiflora, using Maximum Entropy Model (MaxEnt). Input data of the model are locations of pine tree stands and their environmental variables such as climate were prepared for the model inputs. Alternative future projections for P. densiflora distribution were conducted with RCP 4.5 and RCP 8.5 climate change scenarios. As results, the future distribution of P. densiflora steadily decreased under both scenarios. In the case of RCP 8.5, the areal reductions amounted to 11.1% and 18.7% in 2050s and 2070s, respectively. In 2070s, P. densiflora mainly remained in Kangwon and Gyeongsang Provinces. Changes in temperature seasonality and warming winter temperature contributed primarily for the decline of P. densiflora., in which altitude also exerted a critical role in determining its future distribution geographic vulnerability. The results of this study highlighted the temporal and spatial contexts of P. densiflora decline in Korea that provides useful ecological information for developing sound management practices of pine forests.