• Journal of Ecology and Environment
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• v.33 no.2
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• pp.133-139
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• 2010

Atmospheric $CO_2$ concentrations have increased exponentially over the last century and, if continued, are expected to have significant effects on plants and soil. In this study, we investigated the effects of elevated $CO_2$ on the growth of Pinus densiflora seedling and microbial activity in soil. Three-year-old pine seedlings were exposed to ambient as well as elevated levels of $CO_2$ (380 and 760 ppmv, respectively). Growth rates and C:N ratios of the pine seedlings were also determined. Dissolved organic carbon content, phenolic compound content, and microbial activity were measured in bulk soil and rhizosphere soil. The results show that elevated $CO_2$ significantly increased the root dry weight of pine seedling. In addition, overall N content decreased, which increased the C:N ratio in pine needles. Elevated $CO_2$ decreased soil moisture, nitrate concentration, and the concentration of soil phenolic compounds. In contrast, soil enzymatic activities were increased in rhizosphere soil, including ${\beta}$-glucosidase, N-acetylglucosaminidase and phosphatase enzyme activities. In conclusion, elevated $CO_2$ concentrations caused distinct changes in soil chemistry and microbiology.

• Preventive Nutrition and Food Science
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• v.11 no.2
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• pp.105-109
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• 2006

Pinus densiflora belongs to the Pinaceae family which has been widely used for health promoting purposes as folk medicine or as a food. Various curative effects of different parts of the pine have been reported including as a remedy for carcinoma. We examined the effects of pine needle water extracts (PNE) on macrophage function using peritoneal macrophage, pre-osteoclast bone macrophage (Raw 264.7 cell) and brain macrophage (C6 microglia). When peritoneal macrophages were treated with various concentrations of PNE ($1{\sim}100{\mu}g/mL$) for 24 hours, phagocytic activity was significantly increased, whereas it had no effect on tumoricidal activity and NO production. However, the treatment of Raw 264.7 with PNE resulted in the enhancement of NO production at high concentration ($100{\mu}g/mL$). Furthermore, the treatment of C6 with PNE increased the production of NO in a concentration-dependent manner, whereas PNE suppressed NO production in $LPS/IFN-{\gamma}-stimulated$ microglia. These results suggest that PNE has differential immunomodulatory effects on macrophages.

• Journal of the Korean Wood Science and Technology
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• v.47 no.4
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• pp.498-508
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• 2019

This study was carried out to investigate the potential of Korean red pine (Pinus densiflora) inner bark extracts as an antidiabetic agent. The ethyl acetate soluble fraction of the bark extracts was chromatographed on a Sephadex LH-20 column to yield five compounds, which structures were elucidated by NMR spectroscopy. The isolated compounds were (+)-catehin, (-)-epicatechin, taxifolin, taxifolin-3'-O-${\beta}$-D-(+)-glucose and $\tilde{n}$-courmaric acid. The antidiabetic activity of the different fractions, including the crude extracts and isolated compounds, was evaluated by ${\beta}$-cells insulin secretion and glucose uptake in skeletal muscle cells. The insulin secretion was 128% for taxifolin at $25{\mu}g/mL$. However, the other samples had no effect on this test. For the glucose uptake activity assay, $1{\mu}M$ insulin and 2 mM metformin were used as controls. Both the crude extract and taxifolin showed relatively low activity values, but the other samples yielded glucose uptake values over 260%. ${\rho}$-courmaric acid showed the highest uptake (270%). The results confirmed that Korean red pine extracts may be used as a hypoglycemic agent.

• Korean Journal of Agricultural and Forest Meteorology
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• v.10 no.2
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• pp.69-71
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• 2008

• Korean Journal of Agricultural and Forest Meteorology
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• v.10 no.1
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• pp.32-34
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• 2008

• The Korean Journal of Ecology
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• v.21 no.3
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• pp.251-255
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• 1998

To elucidate the effects of a hydroelectric dam construction on annual ring growth of Pinus densiflora, the annual ring widths of 68 trees collected from 7 sites were closely examined. The result was analyzed to ask three special questions first, whether there are real effects of dam construction on the radial growth second, would the magnitude of the effect be different due to two periods of under-construction and post-construction the last, would it be different among age classes. Annual ring growth has been significantly enhanced by the dam construction. Specially, its effect was marked after the construction was finished and the reservoir was filled up with water. There was, however, no remarkable evidence that pine growth would be reduced while the dam was under construction, even though there was a minor decreasing trend. The magnitude of the effect was different among age groups. That is, the changed condition after the construction enhanced relatively the growth of aged trees monre. According to other meterological research since the dam was constructed, the reasons of enhancing pine growth assumed to be the increase of daily temperature, the decreased of daily temperature difference and the increased of rainy days.

• The Korean Journal of Ecology
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• v.10 no.3
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• pp.151-159
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• 1987

Relationships between soil factors and the growth of annual ring of Pinus densiflora grown on stony mountain were investigated at two sites of the different parent rocks: the one was formed by granite at Mt. Gwanag, Seoul and the other feldspar porphyry at Mt. Bipa, Daegu. The growth of annual ring was influenced by the physical factors of soil, such as soil depth, field caacity and water content of soil, rather than by the KDICical factors, such as total nitrogen, potassium, and calcium of soil. Of the soil factors affecting the growth of annual ring, soil depth, field capacity, water content of soil and organic matter closely interrelated with each other. All of these factors influenced water content of soil which might affect the water potential of Pinus densiflora leaves. In fact, the leaf water potential, affecting as the main factor for the growth of annual ring, of the pine grown in a deep soil was higher than that of the pine in a shallow soil.

• Journal of the Korean Wood Science and Technology
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• v.24 no.2
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• pp.81-93
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• 1996

To elucidate the structure of procyanidin in Korean red pine (Pinus densiflora S. et Z.), bark, the extractives were extracted with acetone-water mixture(7:3, v/v) from inner bark of Korean red pine. The extracts separated three fractions which were extracted by liquid-liquid extraction. The extracting solvents were chloroform and ethyl acetate and water. The part of ethylacetate soluble was chromatographed by liquid chromatography. The ethylacetate soluble portion yielded four natural procyanidin dimers, two known epicatechin-($4{\beta}{\rightarrow}6$)-catechin, catechin-($4{\alpha}{\rightarrow}8$)-catechin and two unknown catechin-($4{\beta}{\rightarrow}6$)-catechin and conformational isomer of epicatechin-($4{\alpha}{\rightarrow}6$)-catechin. The additional catechins was also isolated. The structures of these procyanidins were elucidated by their $^{13}C$-NMR spectra.

• Journal of the Korea Furniture Society
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• v.17 no.3
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• pp.23-28
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• 2006

An experiment was conducted to investigate the pit structure of four kind of pine wood species grown in Korea. Torus diameter, margo width, margo lattice size, diameter of pit aperture and pit border width were taken under consideration for explaining the pit structure difference among Pinus densiflora, Pinus rigida, Pinus koraiensis and Larix kaempferi. Torus diameter was found highest in Pinus rigida and the lowest in Pinus densiflora. Margo lattice size varied with torus diameter. Wider torus lowered the margo lattice size. Highest margo width was found in Pinus rigida while the lowest one was found in Pinus koraiensis. Pit aperture diameter was found highest in Pinus densiflora and lowest in Pinus koraiensis. In Pinus rigida, pit border was found the highest and the lowest was found in Larix kaempferi. Pit aperture diameter and pit border were increased gradually from pith to bark while there was a decreasing trend in the margo lattice size measuring from the pith to bark.

• Journal of the Korean Wood Science and Technology
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• v.22 no.3
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• pp.59-67
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• 1994

The essential oils of leaves and resins from P. densiflora and P. koraiensis were analyzed to identify their components. After each retention times of 45 known terpenoids were dertermined with a fixed analytical condition by GC the essential oil compounds of leaves and resins were identified by comparing their retention times with the retention times of known standards. To confirm these results the essential oil components of leaves from P. koraiensis were analized by 2 different GC/MS. According to these results, 36 terpenoids in essential oils of leaves from P. densiflora and P. koraiensis were identified and 15 terpenoids and 22 terpenoids were identified from P. koraiensis resin and P. densiflora resin, respectively. The major components which are more than 2% of total amaunt of volatile components were as follows: 1. The major terpenoids of leaves from red pine. ${\alpha}$-pinene, camphene, ${\beta}$-pinene, D-limonene, ${\beta}$-phellandrene, myrcene, terpinolene, ${\alpha}$-terpineol. 2. The major terpenoids of leaves from korean pine. ${\alpha}$-pinene, camphene, myrcene, D-limonene, 3-carene, terpinolene, bornyl acetate, ${\beta}$-caryophyllene, ${\alpha}$-terpineol, borneol, ${\delta}$-cardinene. 3. The major terpenoids of resin from red pine. ${\alpha}$-pinene, ${\beta}$-pinene, myrcene, ${\beta}$-phellandrene, linalool, linalyl acetate. 4. The major terpenoids of resin from korean pine. ${\alpha}$-pinene, ${\beta}$-pinene, D-limonene, ${\beta}$-caryophyllene, phytol.