• Title/Summary/Keyword: pigment maintenance

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Behavior of Retinal Pigment Epithelial Cells Cultured on Silk Films (실크필름에 배양한 망막색소상피세포의 거동)

  • Lee, So Jin;Kim, Hye Yun;Kim, Seul Ji;Yang, Jaewon;Lee, Seon Ui;Park, Chan Hum;Joo, Choun-Ki;Khang, Gilson
    • Polymer(Korea)
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    • v.38 no.3
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    • pp.364-370
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    • 2014
  • The retinal pigment epithelium (RPE) plays an important role in maintaining a healthy retina and the degeneration of RPE caused a number of retinal diseases. The transplantation of RPE has recently become a possible therapeutic modality for retinal degeneration. To transplant RPE cells securely, substrates are essential, and then as a substrate, we fabricated films using silk that has unique mechanical properties and biocompatibility. After the FTIR spectra, contact angle and biodegradation of silk films were confirmed, RPE cells were seeded and the influence of RPE cells on silk films was examined. We measured the cell adhesion, cell viability, morphology and specific mRNA expression by MTT assay, SEM, immunofluorescence and RT-PCR. In this study, we confirmed that attachment, proliferation and phenotype maintenance of RPE cells cultured on silk films were great, and thereby we were able to confirm the potential applications of silk films as tissue engineering carrier for regeneration of retina.

A Development of concrete Pavement Material with Low Shrinkage and Reflection, High Strength and Performance (저수축 저반사 고강도 고내구성 콘크리트 포장재료 개발)

  • Kim, Hyo-Sung;Nam, Jeong-Hee;Eum, Ju-Yong;Cho, Yoon-Ho
    • International Journal of Highway Engineering
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    • v.11 no.1
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    • pp.13-24
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    • 2009
  • This study developed a high strength and performance concrete pavement material with low shrinkage and reflection of sunlight. Based on the literature review, a new mix-design of applying flash ash to improve the strength and performance of the concrete as well as to reduce the dry shrinkage is suggested. In addition, adding black pigment to reduce the reflection and technique of applying OAG (Optimized Aggregate Gradation) is also included. The result of the laboratory experiment indicates that the brightness and the reflection, which depends on the ratio of black pigment addition, did not deviate from the normal range. When OAG is considered for the mix-design, the strength and performance of the concrete improved greatly. In addition, the mix-design using fly ash reduced the dry shrinkage of concrete and improved the resistance to the permeation of chloride ion. Furthermore, the mix-design, which uses fly ash (25% replacement) and black pigment (3% addition) with the application of OAG, is found to be the most effective mix to reduce the shrinkage and reflection as well as improving the strength and performance of the concrete. The result of an economic analysis indicates that the initial construction cost of this proposed mix is more expensive than that of normal concrete pavement material. However, it can be more economic in the long run because the normal concrete pavement material is likely to cost more due to higher probability of maintenance and repair and higher social cost due to traffic accident, etc.

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Evaluation of Two Biologically Active Compounds for Control of Wheat Root Rot and its Causal Pathogens

  • Hashem, Mohamed;Hamada, Afaf M.
    • Mycobiology
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    • v.30 no.4
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    • pp.233-239
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    • 2002
  • The main aim of this study is to evaluate the efficiency of two biologically active compounds(Strom and F-760) in control of wheat root rot disease and its causal organisms. Fusarium graminearum, F. oxysporum, F. solani and Bipolaris sorokiniana were used as target organisms. In vitro, the two compounds showed fungicidal effect on all investigated pathogens resulted in suppression of radial growth and mycelial dry weight of them. Under greenhouse conditions, treatment of wheat grains with either Strom or F-760 before cultivation significantly reduced the percent of disease distribution as well as the mean disease rating of plants in both seedling and flowering stages. Fresh and dry weights of plants as well as water maintenance capacity were increased as the result of applying these compounds as seed dressing. Also data showed that the membrane stability of plants was injured as a result of infection with all investigated organisms, while this injury was alleviated when F-760 and Strom were applied. The $K^+$ efflux and the leakage of UV absorbing metabolites was stimulated with fungal infection. However, F-760 and Storm treatment partially retarded the stimulatory effect on leakage of $K^+$ and UV-absorbing metabolites of fungal infected plants. On the other side, the fungal infection had inhibitory effects on pigment fractions(chlorophyll a, b, and carotenoids) biosynthesis in wheat leaves. This retarding effect was partially or completely alleviated as the grains were treated with the applied compounds.

Interpretation of Making Techniques through Surface Characteristic Analysis and Non-destructive Diagnosis for the Gilt-bronze Seated Buddha in Dangjin Sinamsa Temple, Korea (당진 신암사 금동여래좌상의 표면특성 분석과 비파괴 정밀진단을 통한 제작기술 해석)

  • CHOI Ilkyu ;YANG Hyeri ;HAN Duru;LEE Chan Hee
    • Korean Journal of Heritage: History & Science
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    • v.56 no.1
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    • pp.100-116
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    • 2023
  • The Sinamsa Temple was built in the late Goryeo Dynasty and a gilt-bronze seated Buddha is enshrined in Geungnakjeon hall in the precinct. Various damages occurred in the gilt layer of the Buddha, such as peeling of the gilt layer and deteriorating gloss. In the study, the conservation conditions of the inside and outside on the statue were accurately investigated, and the making technique was interpreted through the material characteristics and non-destructive diagnosis of the statue. As a result, it is estimated that gold-gilding layer is pure gold, coloration pigment of black is carbon, green is malachite, atacamite and verdigris, red is red lead and cinnabar, respectively. In the deterioration evaluation, peeling, cracking, break out and exfoliation of the gilt layer are confirmed as damages, but the conservation condition is relatively wholesome. However, the gloss of the gilt layer is calculated to be wider in the poorer part than the maintenance part. The ultrasonic velocity of the statue was calculated to be 1,230 to 3,987 (mean 2,608) m/s and showed a relatively wide range. In infrared thermography, peeling was not confirmed, and no special bonding marks were found. In endoscope, some biological damage and corrosion were observed on the surface of the internal metal, and sealed artifacts were identified. Manufacturing technique based on the study, it is considered that the gilt-bronze seated Buddha was cast at once, and the mold was inverted to inject molten metal.