• Title/Summary/Keyword: picB

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Analysis of Microsatellite DNA Polymorphism for Parentage Testing in Dog Breeds (개의 친자감정을 위한 Microsatellite DNA 다형성 분석)

  • Cho, G. J.;Cho, B. W.;Kim, S. K.;Lee, K. W.;Kim, Y. K.
    • Journal of Animal Science and Technology
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    • v.45 no.2
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    • pp.191-198
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    • 2003
  • This study was carried out to investigate a usefulness of the microsatellite DNA markers for individual identification and parentage verification in three dog breeds. A total of 59 random dog (31 Chiwawa, 20 Poongsan, 8 Labrador Retriever) samples were genotyped by using 14 markers (Chiwawa dog), 16 markers (Poongsan dog), and 12 markers (Labrador Retriever dog) among the 17 international standard markers (PEZ1, 3, 5, 6, 8, 10, 11, 12, 13, 15, 16, 17, 20, 21, FHC2010, FHC2054 and FHC2079), respectively. The number of alleles per locus varied from 4 to 14 with a mean value of 6.07 in Chiwawa dog, 2 to 9 with a mean of 4.75 in Poongsan dog, and 3 to 5 with a mean of 4.00 in Labrador Retriever dog. Observed heterozygosity was ranged 0.419${\sim}$0.968 (mean 0.755), 0.300${\sim}$0.950 (mean 0.597) and 0.125${\sim}$0.750 (mean 0.604), and expected heterozygosity was ranged 0.432${\sim}$0.883 (mean 0.711), 0.262${\sim}$0.817 (mean 0.559) and 0.425${\sim}$0.808 (mean 0.660) in these three dog breeds. PIC value was ranged 0.397${\sim}$0.856 (mean 0.659), 0.222${\sim}$0.772 (mean 0.503) and 0.354${\sim}$0.717 (mean 0.563) in these three dog breeds. Of the 17 markers, PEZ1, PEZ3, PEZ6, PEZ10, PEZ12 loci, PEZ1, PEZ6, PEZ13 loci, and PEZ8, PEZ12 loci have relatively high PIC value (>0.7) in Chiwawa dog, Poongsan dog and Labrador Retriever dog, respectively. The exclusion probability was ranged 0.240${\sim}$0.741, 0.111${\sim}$0.616, and 0.198${\sim}$0.529, and the combination of microsatellite loci was 0.9999, 0.9991, and 0.9968 in Chiwawa dog, Poongsan dog and Labrador Retriever dog, respectively. These results can give basic information for developing parentage verification and individual identification system in these three dog breeds.

Studies on the Effect of Picolines on the Stereochemistry of Lanthanide(III) Nitrate Coordination Compounds of 4[N-Furfural)amino]antipyrine Semicarbazone and Antibacterial Activities (4[N-Furfural)amino]antipyrine Semicarbazone의 질산 란탄(III) 배위화합물의 입체화학에 미치는 Picolines의 영향과 항박테리아 활성)

  • Agarwal, Ram K.;Agarwal, Himanshu;Prasad, Surendra;Kumar, Anil
    • Journal of the Korean Chemical Society
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    • v.55 no.4
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    • pp.594-602
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    • 2011
  • The effect of ${\alpha}$-, ${\beta}$- and ${\gamma}$-picolines on the stereochemistry of the coordination compounds of lanthanide(III) nitrates derived from 4[N-(furfural)amino]antipyrine semicarbazone (FFAAPS) has been studied. The general composition of the present coordination compounds is [Ln(FFAAPS)$(NO_3)_3$Pic] (Ln=La, Pr, Nd, Sm, Gd, Tb, Dy or Ho and Pic=${\alpha}$-, ${\beta}$- or ${\gamma}$-picolines). All these coordination compounds have been characterized by elemental analyses, molecular weight, molar conductance, magnetic susceptibility, infrared and electronic spectra. The infrared studies suggest that the FFAAPS behaves as a neutral tridentate ligand with N, N, O donor while ${\alpha}$-, ${\beta}$- or ${\gamma}$-picoline is coordinated to the lanthanide(III) ions via heterocyclic N-atom. Nitrates are bicovalently bonded in these compounds. From the electronic spectral data, nephelauxetic effect (${\beta}$), covalence factor ($b^{1/2}$), Sinha parameter (${\delta}%$) and the covalence angular overlap parameter (${\eta}$) have been calculated. Thermal stabilities of these complexes have been studied by thermogravimetric analysis. The coordination number of lanthanide(III) ions in the present compound is found to be ten. The antibacterial studies screening of the primary ligand FFAAPS and the complexes showed that the present complexes have moderate antibacterial activities.

Effects of Different Sources of Dietary Chromium on Growth, Blood Profiles and Carcass Traits in Growing-finishing Pigs

  • Park, J.K.;Lee, J.Y.;Chae, B.J.;Ohh, S.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.11
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    • pp.1547-1554
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    • 2009
  • This study was carried out to evaluate the effects of dietary supplementation of different sources of chromium on growth performance, blood profile and carcass trait in growing-finishing pigs. A total of 200 growing pigs (Landrace${\times}$Yorkshire)${\times}$Duroc, average initial weight 8.5 kg) were allotted to 5 treatments with 4 replicates per treatment and 10 pigs per replicate. Five treatments were designated as follows according to the source of chromium. i) Control (No chromium): corn-soybean meal based basal diet, ii) $CrCl_{3}$: control diet+200 ppb Cr as $CrCl_{3}$, iii) CrPic: control diet+200 ppb Cr as Cr picolinate, iv) CrMet-1: control diet+100 ppb Cr as Cr methionine, and v) CrMet-2: control diet+200 ppb Cr as Cr methionine. After the feeding trial, three pigs per replicate (12 pigs per treatment) were slaughtered for the evaluation of carcass traits. Average daily gain (ADG), average daily feed intake (ADFI), and feed: gain ratio (F/G) were not different (p>0.05) among dietary Cr sources. However, whole-period ADG of pigs fed CrPic, CrMet-1 and CrMet-2 diets was higher (p<0.05) than for the control diet. Nutrient digestibility was not different (p>0.05) among dietary Cr sources, but the nutrient digestibility of pigs fed CrPic, CrMet-1 and CrMet-2 diets was higher (p<0.05) than for the control diet. BUN level decreased with more magnitude (p<0.05) in pigs fed Cr during the 20 to 50 kg period. Although both serum cholesterol and triglyceride were different (p<0.05) among treatments, there was no consistent response that could be related to the dietary Cr sources regardless of growth phase. However, the overall data suggested that serum cholesterol level increased as BW of pigs increased. Blood total protein (TP) increased (p<0.05) in pigs fed Cr only during the 90-110 kg phase, and blood creatinine (Creat) level was higher in $CrCl_{3}$ and CrPic treatments than in the control only during the 90-110 kg phase. Backfat thickness was thinner (p<0.05) in pigs fed CrMet-2 than in the control treatment. Therefore, lean percentage was higher (p<0.05) in CrMet-2 than in control pigs. However, dressing percentage and Longissimus muscle area (LMA) were not different (p>0.05) among treatments. In conclusion, dietary supplementation of 200 ppb Cr, via either CrPic or CrMet, improved pig growth performance and nutrient digestibility. Moreover, dietary CrMet supplementation for the growing-finishing pig is evidently remarkable for improving both lean percentage of the carcass and backfat thickness.

Optimal Interference Rejection Weight for Multistage Parallel Nulling-Partial PIC Receiver for MIMO MC-CDMA Systems (MIMO MC-CDMA 시스템을 위한 다단계 병렬 널링 및 부분 간섭 제거 수신기를 위한 최적 가중치 결정)

  • 구정회;김경연;심세준;이충용
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.41 no.11
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    • pp.9-15
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    • 2004
  • We propose optimal interference rejection weight for multistage parallel nulling (MPN) partial parallel interference cancellation (PPIC) receiver previously proposed to enhance the performance of V-BLAST for downlink multiple-input multiple-output (MIMO) multicarrier (MC)-code division multiple access (CDMA) systems. MPN-PPIC method proposed in [1] was based on the parallel interference cancellation (PIC) with fixed interference rejection weight obtained experimentally. However, the fixed weight can not be adapted to various systems efficiently, thus we proposed method for the optimal interference rejection weight based on the received signal to interference and noise ratio (SINR), and the performance of the proposed method was evaluated through computer simulation comparing with the previous method. We obtained performance gains of 2.5 dB ~ 5 dB for BER of 10$^{-3}$ .

Interference Canceller Wideband CDMA Systems for IMT-2000 (IMT-2000을 위한 Wideband CDMA시스템에서의 Interference Canceller)

  • 윤헌영;김낙명;문두영
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.8B
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    • pp.1371-1382
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    • 2000
  • IMT-2000 system is the third generation mobile communication system which aims at mobile multimedia services with global roaming. Like 2nd generation system, the IMT-2000 system is also under strong influence of interferences among active subscribers in the multiple access environment. That is, in multiple access environment, signals from one subscriber could interfere with others in the same cell or in the neighboring cells. The interference control in the IMT-2000 systems is much more difficult then the interference control in the 2ndgeneration CDMA cellular systems, since the transmitted power of a subcriber might be different that of others in the IMT-2000 system. In this paper, a parallel interference cancellation with traffic discrimination is proposed which can effectively cancel interferences by grouping the subscribers into several equi-power classes. As a conclusion, simulation results show that the proposed method outperforms the conventional partial PIC scheme in several aspects.

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Triangular Ring Resonator Without Direct Coupling Between Two Access Waveguides of Multimode Interference Coupler (다중모드 결합기의 입출력 광도파로 사이에 광결합이 없는 삼각형 링 공진기)

  • Kim, Doo-Gun;Kim, Hyo-Jin;Kim, Seon-Hoon;Ki, Hyun-Chul;Kim, Hwe-Jong;Oh, Geum-Yoon;Choi, Young-Wan
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.59 no.1
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    • pp.123-128
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    • 2010
  • We have investigated the properties of the novel triangular ring resonator with the total internal reflection mirrors and the semiconductor optical amplifier for photonic integrated circuits (PIC). A novel triangular resonators containing active and passive sections are fabricated and characterized with various multimode interference (MMI) lengths. The optimum MMI length and width turn out to be 108 and 9 ${\mu}m$, respectively. A free spectral range of approximately 228 GHz is observed near 1558 nm along with an on-off ratio of 9 dB. The proposed triangular resonator has a good advantage to remove the direct coupling between the two access waveguides of the MMI coupler. Hence, such resonators can be directly integrated with other devices making compact and highly functional PIC possible.

Microsatellite DNA Typing Using 16 Markers for Parentage Verification of the Korean Native Horse

  • Cho, G.J.;Cho, B.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.6
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    • pp.750-754
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    • 2004
  • This study was performed for parentage verification of the Korean native horse (KNH). 103 random KNH samples (including 19 foals for parentage testing) were genotyped by using 16 microsatellite markers. The number of alleles per locus varied from 5 to 13 with an average value of 8.56 in the KNH. The observed heterozygosity and the expected heterozygosity ranged 0.398-0.893 (the average value was 0.683) and 0.368-0.871 (the average value was 0.727) in the KNH, respectively. The PIC value and the exclusion probability ranged 0.347-0.853 (the average value was 0.692) and 0.208-0.736, respectively, and the total exclusion probability of 16 microsatellite loci was 0.9999. Of the 16 markers, AHT4, AHT5, ASB2, ASB17, HMS2, HMS3, HTG10, LEX33, TKY321 and VHL20 loci have a relatively high PIC value (>0.7) in the KNH. Of the 19 foals, 5 foals were disqualified by an incompatibility of 4-7 markers according to a Mendelian fashion in the present DNA typing for parentage testing. These results present basic information for developing a system for parentage verification and individual identification in the KNH.

Genetic Diversity of Wild Quail in China Ascertained with Microsatellite DNA Markers

  • Chang, G.B.;Chang, H.;Liu, X.P.;Zhao, W.M.;Ji, D.J.;Mao, Y.J.;Song, G.M.;Shi, X.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.12
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    • pp.1783-1790
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    • 2007
  • The genetic diversity of domestic quail and two wild quail species, Japanese (Coturnix coturnix)and Common quail (Coturnix japonica), found in China was studied using microsatellite DNA markers. According to a comparison of the corresponding genetic indices in the three quail populations, such as Polymorphism Information Content (PIC), Mean Heterozygosity ($\bar{H}$) and Fixation Index, wild Common quail possessed rich genetic diversity with 4.67 alleles per site. Its values for PIC and $\bar{H}$ were the highest, 0.5732 and 0.6621, respectively. Domestic quail had the lowest values, 0.5467 and 0.5933, respectively. Wild Japanese quail had little difference in genetic diversity from domestic quail. In addition, from analyses of the fuzzy cluster based on standard genetic distance, the similarity relationship matrix coefficient between wild Japanese quail and domestic quail was 0.937, and that between wild Common quail and domestic quail was 0.783. All of these results showed that the wild Japanese quail were closer to the domestic quail for phylogenetic relationship than wild Common quail. These results at the molecular level provide useful data about quail's genetic background and further supported the hypothesis that the domestic quail originated from the wild Japanese quail.

Optimization of Bovine Testicular PH-20 hyaluronidase Production in Pichia pastoris (소의 히아론산 분해효소(PH-20)의 Pichia pastoris에서의 생산 최적화)

  • Shin, Hwa Shook;Kim, Eunki
    • Korean Chemical Engineering Research
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    • v.46 no.4
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    • pp.764-768
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    • 2008
  • Bovine testicular hyaluronidase PH-20 was cloned into pPIC9 vector and expressed in Pichia pastoris. Recombinant PH-20 was 75 kDa MW and 7460 units/L activity. Extracellular hyaluronidase activity was two times higher than that of intracellular activity. Non-buffered medium and $30^{\circ}C$ cultivation was favorable for PH-20 production. 1M sorbitol as an osmotic pressure and 0.3% methanol inducer increased cell growth and enzyme activity. 0.4 M arginine augmentation decreased the proteolytic degradation of recombinant hyaluronidase.

Efficient Symbol Detection Algorithm for Space-frequency OFDM Transmit Diversity Scheme (공간-주파수 OFDM 전송 다이버시티 기법을 위한 효율적인 심볼 검출 알고리즘)

  • Jung Yun ho;Kim Jae seok
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.30 no.4C
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    • pp.283-289
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    • 2005
  • In this paper, we propose two efficient symbol detection algorithms for space-frequency OFDM (SF-OFDM) transmit diversity scheme. When the number of sub-carriers in SF-OFBM scheme is small, the interference between adjacent sub-carriers may be generated. The proposed algorithms eliminate this interference in a parallel or sequential manlier and achieve a considerable performance improvement over the conventional detection algorithm. The bit error rate (BER) performance of the proposed detection algorithms is evaluated by the simulation. In the case of 2 transmit and 2 receive antennas, at $BER=10^{-4}$ the proposed algorithms achieve the gain improvement of about 3 dB. The symbol detectors with the proposed algorithms are designed in a hardware description language and synthesized to gate-level circuits with the $0.18{\mu}m$ 1.8V CMOS standard cell library. With the division-free architecture, the proposed SF-OFDM-PIC and SF-OFDM-SIC symbol detectors can be implemented using 140k and 129k logic gates, respectively.