• 한국균학회지
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• 제24권1호통권76호
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• pp.49-55
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• 1996

살충성진균 Metarhizium anisopliae가 시들음병을 일으키는 Fusarium oxysporum, 잿빛곰팡이 병을 일으키는 Botrytis cinerea, 겹둥근무늬병을 일으키는 Alternaria solani 등에 대하여 항진균활성을 갖는지 알아보았다. 그 결과 Plate상에서 균사생장저해 실험과 inhibition rate실험을 했을 때 항진균활성이 검정되었으며 또한 YPD배지에서 배양된 M. anisopliae로부터 얻은 배양여액을 식물병원균에 30%농도로 첨가했을 때 이들의 포자발아율은 B. cinerea, F. oxysporum에서 각각 21.5%(control: 88.2%), 53.0%(control: 78.6%)로 감소하였으며 발아개시 시간도 8시간 정도 지연되었다. 또한 현미경을 통한 미세구조관찰에서는 10% 배양여액을 첨가했을 때 F. oxysporum의 균사로부터 후막포자가 생성되고 생장저해가 일어나는 것이 확인되었다. 이상의 결과로부터 M. anisopliae의 항진균활성은 fungistatic effect를 나타냄을 알 수 있었다.

• 한국유전체학회:학술대회논문집
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• 한국유전체학회 2002년도 The 11th Korea Genome Conference
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• pp.48.2-48.2
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• 2002

• Bulletin of the Korean Chemical Society
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• 제28권10호
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• pp.1803-1806
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• 2007

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.21-22
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• 2003

Fungicide treatment is the most important method for the control of plant diseases caused by phytopathogenic fungi. But fungicide resistant strains have appeared in many phytopathogenic fungi. Until now, molecular mechanisms of fungicide resistance such as mutation of target protein, overproduction of target enzyme and detoxification of fungicide have been designated. Recently, it was demonstrated that active efflux of fungicides mediated by ATP-binding cassette (ABC) transporters also contributes to fungicide resistance in several filamentous fungi, such as Aspergillus nidulans, Penicillium digitatum and Botrytis cinerea.(중략)

• 농약과학회지
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• 제2권1호
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• pp.40-45
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• 1998

온실 토양으로부터 주요 작물 병원균의 생육을 억제하는 길항곰팡이를 분리, 동정하였고, 그 균이 분비하는 향균물질을 분리하여 활성을 petriplate assay로 검정했다. 길항균의 foot cell, 분생포자 및 MY20 배지상의 균사 등 형태학적 특징에 근거하여 이 균을 Aspergillus terreus로 동정하였다. A. terreus의 배양여액을 chloroform으로 추출하고, 그 추출물을 column chromatography와 thin layer chromatography로 향균물질을 순수분리하였으며, 화학구조는 butyrolactone I이었다. 이 물질의 주요 작물병원균에 대한 $ED_{50}$은 Botrytis cinerea, Phytophthora capsici. Pythium ultimum, Rhizoctonia solani, 그리고 Fusarium oxysporum에 각각 9.7, 42.6, 23.3, 13.7, 102.7 ppm으로 나타났다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.303.1-303
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• 1998

No Abstract, See Full Text

• 농업과학연구
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• 제29권2호
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• pp.91-97
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• 2002

식물병원균 Bipolaris sorokiniana 배양 추출물로부터 이탈리안 라이그라스 및 논피의 유근신장을 억제하는 활성물질을 분리 정제하여 각종 기기분석을 통하여 화학구조를 결정하였다. 이들 화합물들은 분자식이 $C_8H_8O_3$인 benzenoid 화합물인 3-methoxybenzoic acid와 3-hydroxy benzoic acid methyl ester로 숙주식물의 유근신장을 1000ppm에서 90% 이상 저해하였다. 이들 화합물은 식물체의 잎과 줄기에서 분리되어 항균활성 및 항류마티스 활성이 있음이 보고된바 있으나 B. sorokiniana로부터 분리되어 제초활성에 관하여 보고하기는 본 논문이 처음이다.

• Molecules and Cells
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• 제38권12호
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• pp.1105-1110
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• 2015

Phleichrome, a pigment produced by the phytopathogenic fungus Cladosporium phlei, is a fungal perylenequinone whose photodynamic activity has been studied intensively. To determine the biological function of phleichrome and to engineer a strain with enhanced production of phleichrome, we identified the gene responsible for the synthesis of phleichrome. Structural comparison of phleichrome with other fungal perylenequinones suggested that phleichrome is synthesized via polyketide pathway. We recently identified four different polyketide synthase (PKS) genes encompassing three major clades of fungal PKSs that differ with respect to reducing conditions for the polyketide product. Based on in silico analysis of cloned genes, we hypothesized that the non-reducing PKS gene, Cppks1, is involved in phleichrome biosynthesis. Increased accumulation of Cppks1 transcript was observed in response to supplementation with the application of synthetic inducer cyclo-(${_L}-Pro-{_L}-Phe$). In addition, heterologous expression of the Cppks1 gene in Cryphonectria parasitica resulted in the production of phleichrome. These results provide convincing evidence that the Cppks1 gene is responsible for the biosynthesis of phleichrome.

• Journal of Microbiology and Biotechnology
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• 제19권6호
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• pp.573-581
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• 2009

The complex enzyme pool secreted by the phytopathogenic fungus Fusarium graminearum in response to glucose or hop cell wall material as sole carbon sources was analyzed. The biochemical characterization of the enzymes present in the supernatant of fungal cultures in the glucose medium revealed only 5 different glycosyl hydrolase activities; by contrast, when analyzing cultures in the cell wall medium, 17 different activities were detected. This dramatic increase reflects the adaptation of the fungus by the synthesis of enzymes targeting all layers of the cell wall. When the enzymes secreted in the presence of plant cell wall were used to hydrolyze pretreated crude plant material, high levels of monosaccharides were measured with yields approaching 50% of total sugars released by an acid hydrolysis process. This report is the first biochemical characterization of numerous cellulases, hemicellulases, and pectinases secreted by F. graminearum and demonstrates the usefulness of the described protein cocktail for efficient enzymatic degradation of plant cell wall.

• The Plant Pathology Journal
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• 제23권2호
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• pp.97-102
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• 2007

A total of 39 essential oils were tested for antifungal activities as volatile compounds against five phytopathogenic fungi at a dose of 1 ${\mu}l$ per plate. Five essential oils showed inhibitory activities against mycelial growth of at least one phytopathogenic fungus. Origanum vulgare essential oil inhibited mycelial growth of all of the five fungi tested. Both Cuminum cyminum and Eucalyptus citriodora oils displayed in vitro antifungal activities against four phytopathogenic fungi except for Colletotrichum gloeosporioides. The essential oil of Thymus vulgaris suppressed the mycelial growth of C. gloeosporioides, Fusarium oxysporum and Rhizoctonia solani and that of Cymbopogon citratus was active to only F. oxysporum. The chemical compositions of the five active essential oils were determined by gas chromatography-mass spectrometry. This study suggests that both E. citriodora and C. cyminum oils have a potential as antifungal preservatives for the control of storage diseases of various crops.