• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.6
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• pp.674-682
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• 2014

The purpose of this study was to identify the effect of applying oriental music therapy and reliability of electroencephalogram(EEG) equipment. The study was approved by the critical trial judge committee from ${\bigcirc}{\bigcirc}$(IRB No. 2013-07) university. In order to measure test-retest reliability for 15 subjects, EEG for same participants were measured using same method mentioned above after 2 hours from the first measurement. Same provider implemented to each person at same time. Firstly, EEG was measured for 5 minutes after the subject with attached electrodes sat on chair comfortably for 10 minutes. Then, the subject was given mental stress using the four fundamental arithmetic operations for 5 minutes, and measured EEG for another 5 minutes. After that the subject sat on the chair comfortably listening oriental medicine music therapy for 5 minutes, and EEG was measured for 5 minutes again. There was no side effect regarding music therapy reported. Raw data, which was measured in each step, were converted through FFT(fast fourier transform) and analyzed after divided into certain frequency including ${\alpha}$ wave, ${\beta}$ wave, ${\theta}$ wave, immersion wave, stabilization wave, sef100 wave, and sef95 wave. Data were analyzed using wilcoxon signed rank test, Intraclass correlation coefficient(ICC), repeated measures ANOVA with the SPSS program. In test-retes method, there were significantly differences in ${\alpha}$ wave, ${\beta}$ wave, immersion wave, stability wave, ${\theta}$ wave, sef100 wave, sef95 wave. ICC has shown a high degree of reliability that it was ${\alpha}$ wave .877, ${\beta}$ wave .855, ${\gamma}$ wave .895, immersion wave .897, stability wave .816, ${\theta}$ wave .904, sef100 wave .910, sef95 wave .776. Also, there was a statistically significant difference in ${\alpha}$ wave after applying oriental music therapy. Based on these results, it is considered that average of the channel EEG and application of oriental music therapy would be practiced by increase of sample size using this machine.

• Applied Microscopy
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• v.31 no.1
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• pp.1-8
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• 2001

The morphological features of neuronal dendritic spines are changed their shapes, sizes and density in response to physiological or pathological conditions . Therefore, exact analysis of spines warrants understanding of neuronal function. The size of the spine is at the borderline of resolution with light microscopy. High voltage electron microscopy Provide excellent resolution of the spines with proper stain techniques thanks to its higher resolution and penetration power. We evaluated more effective staining method for observing dendritic spines after labeling Purkinje cells with anti-calbindin 28 kD immunohistochemistry or Golgi staining methods. 4 fm thickness sections were observed with high voltage electron microscopy and some morphometric analyses were performed. Both Golgi staining and immunohistochemistry revealed the detail structures of the Purkinje cell such as soma, dendrites, and dendritic spines. High voltage electron micrographs with Golgi staining provide more precise morphology and are easy to measure. Average density of spine is $24.5{\pm}3.6/10{\mu}m$ and its length is $1.12{\pm}0.22{\mu}m$. For quantitative analysis of the spines, high voltage electron, micrographs with Golgi staining are more effective. This preliminary result is expected to be useful for further study of spine plasticity in various conditions.

• Environmental Analysis Health and Toxicology
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• v.11 no.1_2
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• pp.19-29
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• 1996

Human exposure to volatile compounds in tap water can occur from inhalation and dermal absorption as well as direct ingestion. The relative contribution to total human exposure from these pathways has been considered to be important especially for VOC's (volatile organic compounds). In an attempt to reduce the uncertainty of the risk assessment, it has been suggested that the exposure assessment process could be significantly improved by adopting Monte-Carlo simulation. However, there is no actual data in Korea for each exposure parameter to determine the level of exposure, and the distributional pattern. Therefore, we surveyed water use patterns and behavior related to multi-route exposure to VOC's in household tap water in Korea, and compared these values to the those in western countries. In the first survey, we calculated daily water intake using data from a sample of 1322 persons of several cities in Korea. In the second survey, we obtained questionnaire data on exposure time for showering, bathing and household activities, and tap water intake from 851households in Korea. In the last survey, we measured the exposure parameters (exposure time, water use rate etc.) related to showers, baths, toilets, dish washing, washing and cooking, and tap water intake was surveyed. Also, the subjects were measured their body weight, height and tidal volume, etc. A diary, a flow meter and a measuring cup were used to measure these values as precisely as possible. Average daily water intake was ranged 0.79-1.71 L/day for adults in three surveys. Tap water intake measured by log-sheet during one week in third survey was 1.26 (average), 1.98 L/day (90 percentlie), respectively. These results were comparable with results from EPA (1.4, 2L/day). The average amount of water used by housewives in the third survey was 515.0 $\pm$ 564.6L/day. In usual activity, the amount of water used in the bathroom, the laundry and the kitchen was 140.0 $\pm$ 538.9, 148.0 $\pm$ 174.5, 229.3 $\pm$ 205.4 L/day, respectively. Exposure parameters such as water intake rate, exposure duration, body weight, inhalation rates in surveyed data of Korean people differed from those published from western countries. This could be attributed to variations in lifestyle, dietary habits and physiological characteristics.

• Korean Journal of Agricultural Science
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• v.39 no.4
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• pp.515-523
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• 2012

Recent development and enlargement of reclaimed lands along the sea shores required to explore alternatives to existing crops, which are suitable to grow well and to declare higher profits. The objectives of this study were to investigate yield-related parameters for development of new leafy vegetables, and to screen some candidates among New Zealand spinach genotypes which were collected in Korea. Initially all the collected genotypes were grown for 7 weeks in a greenhouse of the experiment field in Chungnam National University, and then transplanted into the field to measure morphological or physiological parameters (plant height, branch number, stem diameter, chlorophyll content, and maximum canopy diameter), and yield-related parameters (leaf number, leaf length, leaf width, fresh weight and dry weight). Those parameters were quantitatively measured at 1, 5 or 9 weeks after transplanting (WAT). Parameters exhibiting statistically significant difference among the accessions were plant height, branch number, maximum canopy diameter, chlorophyll content, and leaf length. In contrast to highly correlated parameters with fresh and dry weight obtained in salt-affected soil in the earlier report, fresh weight was correlated significantly with leaf length (r=0.72), leaf width (r=0.64), and canopy diameter(r=0.66), while dry weight was correlated significantly with plant height (r=0.46), leaf length (r=0.72), leaf width (r=0.73), fresh weight (r=0.79), chlorophyll content (r=0.47), and canopy diameter (r=0.87). Based on the significantly correlated with yield parameters, the candidate accessions which were ranked in top statistical groups include CNU06A01, CNU06A13, CNU06A26, CNU06A35, CNU06A38, and CNU06A55. In order to be cultivated in reclaimed lands, it is necessary to screen out salt tolerant accessions among the above high-yielding genotypes.

• Investigative Magnetic Resonance Imaging
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• v.2 no.1
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• pp.73-82
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• 1998

Purpose : A precise NMR technique for measuring the rate of water exchange and cell membrane permeability across the hepatocyte membrane using liver-specific MR contrast agent is described. Materials and Methods : The rat hepatocytes isolated by perfusion of the livers were used for the NMR measurements. All experiments were performed on an IBM field cycling relaxometer operating from 0.02MHz to 60 MHz proton Larmor frequency. spin-echo pulse sequence was empolyed to measure spin-lattice relaxation time, T1. The continuous distribution analysis of water proton T1 data from rat hepatocytes containing low concentrations of the liver specific contrast agent, Gd-EOB-DTPA, modeled by a general two compartment exchange model. Results : The mean residence time of water molecule inside the hepatocyte was approximately 250 msec. The lower limit for the permeability of the hepatocyte membrane was $(1.3{\pm}0.1){\;}{\times}{\;}10^{-3}cm/sec$. The CONTIN analysis, which seeks the natural distribution of relaxation times, reveals direct evidence of the effect of diffusive exchange. the diffusive water exchange is not small in the intracellular space in the case of hepatocytes. Conclusions : Gd-EOB-DTPA, when combined with continuous distribution analysis, provides a robust method to study water exchange and membrane permeability in hepatocytes. Water exchange in hepatocyte is much slower thatn that in red blood cells. Therefore, tissue-specific contrast agent may be used as a functional agent to give physiological information such as cell membrane permeability.

• Journal of Korean Academy of Nursing
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• v.25 no.3
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• pp.538-548
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• 1995

Exercises are achievement oriented, the process is frequently perceived as hard and difficult Participants drop out from exercise programs in the middle of the training period. Dance movement, which is the deliberate and systematic use of movement, is enjoyable during the movement and provides opportunities for persons to express them-selves. Regular long term dance movement may in-duce a training effect with a decreased drop out rate. Dance movement could be one way to attain wellness, however, there have been few studies to evaluate both physiological and psychological aspects of dance movement. This study focused on evaluating the effects of dance movement training on body weight, resting blood pressure and heart rate, limb circumference and strength, stress response and subjective feelings. This quasi-experimental study was designed as a nonequivalent control group pre test -post test study. Ten healthy fe-male subjects, aged between 19 and 31 years volunteered for an eight week dance movement program. Ten healthy female subjects, between 19 and 21 years of age paticipated as controls. None of the subjects had performed regular physical activity for six months prior to the study. Dance movement was created with reference to Heber's movement guide. The Dance movement program consisted of approximately 30 minutes of dance, three days per week, for eight weeks. During each 30 minute work out, there were approximately 5 minutes of warm-up dancing, 20 minutes of conditioning dance and 5 minutes of cool-down dancing. The intensity for the conditioning phase was at between 60% and 65% of age-adjusted maximum heart rates. Body weight, resting blood pressure and heart rate, circumference of mid upper arm, mid thigh and mid calf, muscle strength of upper and lower limb, physical and psychological response to stress were measured prior to, and following the experimental treatment. Body weight was measured by digital weight scale(Kyung In Corp., Korea). Resting systolic and diastolic blood pressure were measured by sphygmomanometer, Resting heart rate was measured for one minute in a relaxed sitting position using the radial artery. Circumference of mid upper arm, mid thigh and mid calf was determined by tape measure. Muscle strength of the upper extremities was measured by a grip dynamometer (Takei Corp. No.1857, Japan) and that of the extremities was measured by the length of time the leg could be held at 45° Physical and psychological responses to stress were measured using the Symptoms of Stress (SOS)Scale. Paticipants in the dance movement were interviewed by the facilitator following the eight weeks, and their thematic responses about the dance movement were recorded. Following the eight week dance movement train-ing, body weight decreased significantly, circumference of mid thigh and mid calf increased. The length of time leg - raising could be held tended to increase following the dance movement training. Resting systolic and resting heart rate showed a tendency to decrease. Total mean score of stress response tended to de-crease, and mean score of habitual patterns, do-pression, anxiety / fear, anger and cognitive disorganization decreased remarkably following the eight week dance movement. Thematic responses about the dance movement were positive following the training.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1221-1225
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• 2009

The pulse diagnosis is an important and universally used method in Oriental Medicine. Since the traditional method of palpating the pulse relies on the subjective sense in the fingers of an Oriental Medical Doctor(OMD), there has been continued need for more objective method for pulse diagnosis. Recently, various pulse analyzers have been developed to meet such objective palpation and interpretation. However, most of these attempts were not successful to replace OMD's own palpation by fingers. To improve the performance of the pulse analyzers, one should develop machine-appropriate interpretations for the pulse images in the literature, in addition to the improvement in the repeatability and reproducibility. One of such widely-used pulse images to be interpreted is the floating and sinking pulse. The floating and sinking pulses are the two representative pulse images informing us how strong pressure one should apply to obtain the maximal pulse strength. A previous study suggested a convenient and unified measure for the floating and sinking pulses by defining the coefficient of the floating-sinking pulse(CFS). We found the original definition of the CFS could be erroneous under some situations. To improve the performance, we introduce new CFS algorithm for determining the floating and sinking pulse with a pulse analyzers(3-D MAC). To test the performance of the newly suggested algorithm, we conducted a clinical study comparing the agreement ratio with the floating and sinking pulse diagnosis by the OMDs. We found that, among the subjects who are diagnosed with having either the floating pulse or sinking pulse, the new CFS algorithm showed 55.3% diagnosis rate and 73.0% concordance rate, which are about 3% and 6% improvement in the diagnosis rate and agreement rate, respectively, compared to the original CFS algorithm.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1341-1348
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• 2009

Hoechunyanggyeok-san (HYS) is a traditional oriental herbal medicine widely used for treating inflammatory disorders. Although there are numerous clinical results of HYS reported in the literature of oriental hebal medicine, it has been rarely conducted to evaluate the immuno-biological activity. The present study was conducted to examine the anti-inflammatory effects of HYS extract (HYSE) in vivo and in vitro. To determine the cytotoxic concentration of HYSE, cell viability was tested by MTT assay. All four doses of HYSE (0.01, 0.03, 0.10 and 0.30 mg/ml) had no significant cytotoxicity during the entire experimental period. In order to measure NO levels in culture medium, the cells were treated with $1\;{\mu}g/ml$ of LPS 1h before adding HYSE for 24 h and then culture medium were reacted with Griess reagent. Increased NO production and iNOS expression were detected in LPS-activated cells compared to control. However, these increases were dose-dependently attenuated by treatment with HYSE. LPS plays a key role in leading to the massive production of pro-inflammatory cytokines such as TNF-$\alpha$, IL-$1{\beta}$ and IL-6 in macrophages. Thus, we next determined the levels of these cytokines. HYSE reduced the elevated production of TNF-$\alpha$, IL-$1{\beta}$ and IL-6 by LPS. Moreover, the effects of HYSE were in a dose-dependent manner. In vivo, histopathological study, HYSE effectively inheefed the increases of hind paw skin thicknesses and inflammatory cell infiltrations induced by carrageenan treatment. It, therefore, considered that HYSE will be favorably inheefed the acute edematous inanner. In s. These findings showed that HYSE could have anti-inflammatory effects through the reduction of NO and inflammatory cytokines in macrophage. Furthermore, the reduction of carrageenan-induced paw oedema by HYSE helps to understand its actions on inflammatory conditions.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1491-1496
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• 2009

Stroke is usually associated with the cerebral blood flow of the central nervous system. However, studies concerning the effects of neurologic sysmptoms induced from stroke on the peripheral blood flow has not taken place sufficiently. To ascertain the feasibility of a blood flow meter adopting to use doppler ultrasonogrphy, under the prospect that hemiparesis induced from stroke may have effect on the peripheral blood flow, the peripheral blood flow velocity was observed. The control group made up of healthy people without any factors capable of effecting the peripheral blood flow velocity, and patient group which consist of hemiparetic people induced from stroke, were recruited. Volumes of recruitment are 21 persons in the patient group, and 29 persons in the control group, but the final numbers of people are 17 and 21 respectively because of the inconsistancy in the method of the test. The non-invasive method of Doppler effect of Ultrasound was used to measure the blood flow velocity. The blood flow velocity in the peripheral part of left and right fourth fingers:dorsal branches of proper palmar digital artery to dorsum of distal phalanges, was measured in the control group and patient group through Doppler Ultrasound. In comparison of the control group and the patient group, the systolic blood flow velocity from the peripheral part of the upper extremity was lower in the patient group than that of the control group. According to such results, it is concluded that hemiparesis induces the reduction of the peripheral blood flow velocity in the systolic phase.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.6
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• pp.4021-4030
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• 2015

Pulse wave is the physiological responses through the autonomic nervous system such as ECG. It is relatively convenient because it can measure the signal just by applying a sensor on a finger. So, it can be usefully employed in the field of U-Healthcare. The objects of this study are acquiring the PPG (Photoplethysmography) one of the way of measuring the pulse waves in non-invasive way using the CMOS image sensor on a smartphone camera, developing the portable system judging stressful or not, and confirming the applicability in the field of u-Healthcare. PPG was acquired by using image data from smartphone camera without separate sensors and analyzed. Also, with that image signal data, HRV (Heart Rate Variability) and stress index were offered users by just using smartphone without separate host equipment. In addition, the reliability and accuracy of acquired data were improved by developing additional hardware device. From these experiments, we can confirm that measuring heart rate through the PPG, and the stress index for analysis the stress degree using the image of a smartphone camera are possible. In this study, we used a smartphone camera, not commercialized product or standardized sensor, so it has low resolution than those of using commercialized external sensor. However, despite this disadvantage, it can be usefully employed as the u-Healthcare device because it can obtain the promising data by developing additional external device for improvement reliability of result and optimization algorithm.