• Title/Summary/Keyword: physiological factors

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Enhancement of Drought-Stress Tolerance of Brassica oleracea var. italica L. by Newly Isolated Variovorax sp. YNA59

  • Kim, Yu-Na;Khan, Muhammad Aaqil;Kang, Sang-Mo;Hamayun, Muhammad;Lee, In-Jung
    • Journal of Microbiology and Biotechnology
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    • v.30 no.10
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    • pp.1500-1509
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    • 2020
  • Drought is a major abiotic factor and has drastically reduced crop yield globally, thus damaging the agricultural industry. Drought stress decreases crop productivity by negatively affecting crop morphological, physiological, and biochemical factors. The use of drought tolerant bacteria improves agricultural productivity by counteracting the negative effects of drought stress on crops. In this study, we isolated bacteria from the rhizosphere of broccoli field located in Daehaw-myeon, Republic of Korea. Sixty bacterial isolates were screened for their growth-promoting capacity, in vitro abscisic acid (ABA), and sugar production activities. Among these, bacterial isolates YNA59 was selected based on their plant growth-promoting bacteria traits, ABA, and sugar production activities. Isolate YNA59 highly tolerated oxidative stress, including hydrogen peroxide (H2O2) and produces superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities in the culture broth. YNA59 treatment on broccoli significantly enhanced plant growth attributes, chlorophyll content, and moisture content under drought stress conditions. Under drought stress, the endogenous levels of ABA, jasmonic acid (JA), and salicylic acid (SA) increased; however, inoculation of YNA59 markedly reduced ABA (877 ± 22 ng/g) and JA (169.36 ± 20.74 ng/g) content, while it enhanced SA levels (176.55 ± 9.58 ng/g). Antioxidant analysis showed that the bacterial isolate YNA59 inoculated into broccoli plants contained significantly higher levels of SOD, CAT, and APX, with a decrease in GPX levels. The bacterial isolate YNA59 was therefore identified as Variovorax sp. YNA59. Our current findings suggest that newly isolated drought tolerant rhizospheric Variovorax sp. YNA59 is a useful stress-evading rhizobacterium that improved drought-stress tolerance of broccoli and could be used as a bio-fertilizer under drought conditions.

Effect of Adenosine Analogues on the Renal Function and Renin Release (가토의 신장기능과 Renin분비에 미치는 Adenosine Analogues의 영향)

  • Song, Yun-Sik;Choi, Byung-Soo;Kim, Suhn-Hee;Cho, Kyung-Woo
    • The Korean Journal of Physiology
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    • v.22 no.2
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    • pp.319-332
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    • 1988
  • The regulations of renal function and renin release are influenced by neural, humoral and physical factors. During the last decade, considerable progress has been made in the identification and characterization of these extrinsic renal control systems. Mechanisms intrinsic to the kidney are also important for renal function. These include the autoregulation of blood flow, and the local control of renin secretion. Fundamental questions regarding the mechanism of these intrinsic controls remain unanswered. Recently, endogenous renal adenosine has been claimed to influence the tubuloglomerular feedback control and renin release. Two subclasses of adenosine receptors $A_1{\;}and{\;}A_2$ have been described. The present experiment was carried out to evaluate the effects of $N_6-cyclohexyladenosine$ $(CHA,{\;}A_1{\;}selective)$ and 5'-N-ethylcarbox-amide adenosine $(NECA,{\;}A_2{\;}selective)$ on the renal function and renin release in the unanesthetized rabbit. Intra-renal arterial infusion of NECA $(0.3{\sim}10.0n{\;}mole/min/rabbit)$ or CHA $(0.03{\sim}10.0n{\;}mole/min/rabbit)$ caused a prompt and dose-dependent decrease in urine volume, glomerular filtration rate (GFR), renal plasma flow (RPF), filtration fraction (FF), electrolyte excretion and free water clearance $(CH_2O)$, the effect being much more profound with CHA than with NECA. The NECA infusion resulted in a profound decrease of systemic blood pressure, but the CHA infusion did not. Both NECA and GHA infusions caused a prompt and dose-dependent decrease in renin secretion rate, again the effect being greater with CHA than with NEGA. These results suggest that both $A_1{\;}and{\;}A_2$ adenosine receptors may be involved in the intrinsic control of renal function and renin release, and that the $A_1$ receptors plays a more important role than the $A_2$ receptor in the regulation of renal fnction.

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Factors Affecting the Lactate Dehydrogenase Activity of a Spore-forming Lactic Acid Bacteria (포자형성 유산균의 lactate dehydrogenase 역가에 미치는 제요인)

  • ;Hah, Yung Chil;Hong Soon Woo;Lee, Jung Chi
    • Korean Journal of Microbiology
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    • v.15 no.3
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    • pp.103-112
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    • 1977
  • Several strains of spore-forming lacticacid bacteria were isolated from natural sources such as soils, cereals, and foods. The general morphological and physiological characteristics of the strain 6-4 were investigated nad compared with some other industrial strains. The effects of fructose-1,6-diphoshpate (FDP), adenosine triphosphate (ATP), and pH on the lactate dehydrogenase(LDH) activity of the strain were studied, and the changes in LDH activity and spore formation under various cultural conditions were researched. The results were as follows. 1. This strain was identified to Bacillus coagulans Hammer and distributed widely in natural sources. 2. The strain strongly converted various fermentation substrates in to L(+)-lacticacid in anaerobic conditioins, and many spores that were of great advantages to the industrial application were formed easily in the aerobic condition. 3. The LDH activity of this strain was activated by FDP and inhibited by ATP. The optimal pH for the enzyme activity was 6.0-6.5. 4. In the anaerobic culture condifion, the large amount of glucose added in the medium increased the LDH activity, but the cells were not committed to sporulate. 5. When none or a very small amount of glucose (less than 0.5%) was added to culture medium in the aerobic condition, the LDH activity was decreased and many spore were produced with final pH higher than 8.5. 6. The additioin of large amount of glucose (more than 2.0%) in aerobic culture increased the LDH activity and inhibited strongly the spore formation with final pH lower than 6.0.

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Effect of Ethylene Scrubber in Polyethylene Film Wrap on the Storage of 'Tsugaru' Apples (플라스틱 필름포장 내 에틸렌제거가 사과 '쓰가루' 과실의 저장 품질에 미치는 영향)

  • Hwang, Yong Soo;Chun, Jong Pil;Lee, Jae Chang
    • Korean Journal of Agricultural Science
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    • v.25 no.1
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    • pp.19-25
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    • 1998
  • This study was conducted to develop an appropriate postharvest technique to extend the shelf-life of an early cultivar, 'Tsugaru' apples. The general quality of stored fruit was more affected by maturity at harvest than postharvest treatments. When firmness and internal quality were considered, fruits harvested at the medium stage of color development showed better results. Between postharvest treatments examined in this study, a simple polyethylene film(PE) wrapping (30um) was excellent on inhibition of weight loss but this treatment revealed no clear effect on other quality factors including firmness and acidity. Ethylene scrubber(ES) in polyethylene wrapping showed a positive effect on keeping fruit quality in general. Especially acid content was higher in fruit stored with PE + ES, thus, fruit seemed to be better in freshness. A physiological disorder was observed regardless postharvest treatments. The symptom was associated with incidence of pitting on surface and breakdown of the effected tissue. And much more symptoms were developed near the calyx end. Thus, future studies concerning 'Tsugaru' storage should include an examination of the optimum storage temperature.

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Importance and production of chilli pepper; heat tolerance and efficient nutrient use under climate change conditions

  • Khaitov, Botir;Umurzokov, Mirjalol;Cho, Kwang-Min;Lee, Ye-Jin;Park, Kee Woong;Sung, JwaKyung
    • Korean Journal of Agricultural Science
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    • v.46 no.4
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    • pp.769-779
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    • 2019
  • Chilli peppers are predominantly cultivated in open field systems under abiotic and biotic stress conditions. Abiotic and biotic factors have a considerable effect on plant performance, fruit quantity, and quality. Chilli peppers grow well in a tropical climate due to their adaptation to warm and humid regions with temperatures ranging from 18 to 30℃. Nowadays, chilli peppers are cultivated all around the world under different climatic conditions, and their production is gradually expanding. Expected climate changes will likely cause huge and complex ecological consequences; high temperature, heavy rainfall, and drought have adverse effects on the vegetative and generative development of all agricultural crops including chilli peppers. To gain better insight into the effect of climate change, the growth, photosynthetic traits, morphological and physiological characteristics, yield, and fruit parameters of chilli peppers need to be studied under simulated climate change conditions. Moreover, it is important to develop alternative agrotechnologies to maintain the sustainability of pepper production. There are many conceivable ideas and concepts to sustain crop production under the extreme conditions of future climate change scenarios. Therefore, this review provides an overview of the adverse impacts of climate change and discusses how to find the best solutions to obtain a stable chilli pepper yield.

The Impact of Late-Night Fits on Utilitarian·Hedonic Value and Shopping intent (심야 적합성이 심야쇼핑의 실용적·쾌락적 가치와 쇼핑의도에 미치는 영향에 관한 연구)

  • Jeong, Yun-Hee
    • Management & Information Systems Review
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    • v.33 no.5
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    • pp.117-130
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    • 2014
  • The purposes of this study were to consider the influence of person-late night fit in the perceived value, intent of late-night shopping. The late-night shopping model which we present in this study incorporates three effects including the time fit and the physiological fit, the mood fit factors. Also, we expect that these fits effect utilitarian value and hedonic value, in turn, utilitarian value and hedonic value effect shopping intent. Survey research is employed to test hypotheses involving late-night fits, values, shopping intent. We collected data involving various late-night shopping, and used 221 respondents to analyze these data using LISREL structural modeling. The proposed model was a good fit with the data(GFI= .96 NNFI= .80 CFI= .92, AGFI= .88, RMR= .044), the hypothesized relationships were partly significant(p< .05). In the final section, we discussed several limitations of our study and suggested directions for future research. We concluded with a discussion of managerial implications, including the potential to advance understanding experiential consumption and implying an enhanced ability to attract late-night shopper.

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Analysis of the Effectiveness of Tai Chi Exercise for improving Balance (타이치 운동 중재 후 균형 증진에 관한 논문분석)

  • Lee, Hea-Young;Suh, Moon-Ja;Lee, Eun-Ok;Eun, Young;Choi, Jung Hyun
    • Korean Journal of Adult Nursing
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    • v.16 no.3
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    • pp.409-420
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    • 2004
  • Purpose: This study aims to analyze the effectiveness of Tai chi exercise for improving balance and its tendency and helps to identify directions for future research. Method: 19 articles from Medline search of foreign journals(1981-2003) and 5 from Korean nursing journals (1981-2003) were surveyed. The contents analyses were focused on outcome measures and relative factors regarding balance. Result: Variable measures for balance were used in Tai Chi studies relating to balance. They included the functional measures such as 14 single-leg stance, 7 walking in physiological measures and 7 laboratory-based balance measures were done with platform stability test. The measure of effective Tai Chi exercise depended on the health status of subjects and the methods. Health status of subjects were only on physically inactive older adults in 17 articles and arthritis patients in 7 articles in the Tai Chi study. The significant effects of Tai Chi exercise on balance was revealed after 10weeks duration. Conclusion: Considering the results, Tai Chi exercise is more effective than any other studies for walking balance. But the reports on the outcome are inconsistent with wide variations in the choice of balance measures. It is suggested that future studies analyze systemic change through meta analysis and have a wide variety of subjects who need postural control and balance control. The methods of measures should be distinct from the balance state of subjects for the effectiveness of Tai chi exercise.

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Effects of Exercise Program for Workers in Small Enterprises (운동프로그램의 효과 -소규모사업장 근로자를 대상으로-)

  • Hyun, Hye-Jin;Yoon, Soon-Nyoung;Choi, Eun-Ok
    • Research in Community and Public Health Nursing
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    • v.12 no.2
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    • pp.338-343
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    • 2001
  • Physical activity is one of important factors of the chronic disease. It was reported that Most of Korean workers didn't take exercise regularly. The purpose of this study is testing effects of exercise program, based on the Theory of Planned Behavior. 30 subjects participating in this study are workers in small enterprises and have not taken exercise regularly. Exercise program, which includes education, support, monitoring, and counseling, was conducted for 6 weeks. Effects of this program was evaluated by the change of exercise, attitude, subjective norm, perceived control. intention of exercise, perceived health states, and fatigue. The results of this study can be summarized as follows: 1. There was the significant difference between pre and post-test in the subjective norm, perceived control. and intention of exercise, but not the significant difference in the attitude of exercise. 2. 53.3% of the subject of this study had taken exercise regulary in the post-test. 3. There was not the significant difference in the perceived health states and fatigue, From the results, it can be concluded that the exercise program is effective, leading to the change of intention and exercise rate. On the other hand, it was not effective in Physiological aspects. It seems that these result was due to short term program. Thus, it can be recommended that studies are needed with longer term program and larger sample.

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Optimal Production of Poly-$\beta$-hydroxybutyrate and Polysaccharide Methylan by Mentylobacterium organophilum from Methanol (메탄올로부터 Methylobacterium organophilum을 이용한 Poly-$\beta$-hydroxybutyrate와 다당류 Methylan의 최적 생산조건)

  • 김재연;김선원
    • KSBB Journal
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    • v.10 no.2
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    • pp.176-182
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    • 1995
  • The environmental and physiological factors affecting the production of exopolysaccharide (Methylan) and Poly-${\beta}$-hydroxybutyrate(PHB) by Methylobacterium organophilum were investigated. The maximum PHB content was obtained at $38^{\circ}C$ whereas maximum polysaccharide concentration was $3.54g/\ell$ at $30^{\circ}C$. Optimum pH was pH 7-8 for PHB production and pH 6-7 for polysaccharide production, respectively. Under the condition of $Mo^{2+}, Mg^{2+} or Mn^{2+}$ limitation with nitrogenlimitation, the PHB accumulation was increased, whereas the polysaccharide production was decreased as compared with that of solenitrogenlimitation. Under the condition of sole K+ limitation, cell growth was significantly inhibited and no polysaccharide was produced. However, the PHB content was as high as 60% of dry cell weight. Effect of C/N ratios (methanol/ammonium) in the feeding solution was examined for the simultaneous production of polysaccharide and PHB. The higher ratio of C/N showed the lower cell growth, higher content of PHB in cells, and higher yield of polysaccharide.

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Hospitalization Stress in Child : Concept Analysis (아동의 입원 스트레스에 대한 개념분석)

  • Lee, Young-Ok
    • Journal of the Korea Convergence Society
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    • v.11 no.1
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    • pp.327-335
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    • 2020
  • The purpose of this study was to investigate conception of children's hospitalization stress and to make clear concepts, and to use Walker & Avant's concept analysis method. Children's hospitalization stress attributes were derived from physiological changes, individual-environmental interactions, cognitive and psychosocial developmental stage responses, and intrinsic and outward changes due to coping styles. The prerequisites for hospitalization stress of children were disease severity, biological factors, and support system, and the results were hospitalization adaptation, disease and health recovery, improvement of child development, and acquisition of coping skills. In order to solve the prerequisites for Hospitalization stress of children, it is necessary to be aware of the disease and to establish a support system of parent-family. Afterwards, we suggest more qualitative research on the hospitalization stress of children, the development of tools that reflect the characteristics of the hospitalization stress of children, and the development of programs to solve the Hospitalization stress.