• Toxicological Research
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• 제21권3호
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• pp.187-193
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• 2005

The rodent Hershberger assay is considered as a potential short term in vivo screening method for the detection of androgenic or anti-androgenic compounds. The objective of this study was to evaluate the anti-androgenic activities of di(2-ethylhexyl) phthalate (DEHP), di(n-butyl) phthalate (DBP), and butylbenzyl phthalate (BBP). A 10-day Hershberger assay was performed using immature Sprague-Dawley male rats castrated at 6 weeks of age. Tastosterone propionate (TP, 0.4 mg/kg/day) was administered s.c. to castrated male rats and followed by flutamide (1, 5, 10, or 20 mg/kg/day) treatment for 10 days by oral gavage. Similarly, DEHP, DBP, or BBP were also administered by oral gavage at 250, 500, or 1000 mg/kg/day after TP (0.4 mg/kg/day) administration. As expected, flutamide significantly inhibited the TP-induced re-growth of seminal vesicles, ventral prostate, and Levator ani plus bulbocavernosus muscles (LABC) at 1 mg/kg/day and above, and Cowper's glands and glans penis at 5 mg/kg/day and above. DEHP significantly (p<0.05) decreased the seminal vesicles, ventral prostate, LABC and Cowper's glands weights at 1000 mg/kg/day. BBP at 1000 mg/kg/day significantly inhibited TP-induced re-growth of the LABC in the immature castrated male rats, whereas ventral prostate, seminal vesicles, and Cowper's glands weights were unaffected. In contrast to DEHP, DBP did not affect accessory sex organ weights at any concentration. Body weights, combined adrenal glands, and kidney weights were not affected, but liver weights were significantly increased at high dosages in the DEHP, DBP, and BBP treatment groups. Our observations strongly suggest that DEHP acts as an androgen antagonist at the high dose (i.e., 1000 mg/kg/day).

• Toxicological Research
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• 제17권2호
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• pp.115-121
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• 2001

Some of endocrine disruptors with sexual hormone-like effects have been increasingly reported to be immunotoxic in many species in recent several years. Phthalate esters have possible effects on the endocrine system. Prenatal exposure to di(n-butyl) phthalate (DBP) has been reported to impair the androgen-dependent development of the male reproductive tract in rat. Therefore, the immunomodulatory effect of DBP was investigated in the developing immune system of fetal and neonatal Sprague-Dawley rats. Timed-bred pregnant SD rats were given to the doses of 0, 250, 500, and 750 mg DBP/kg$\cdot$ body weight /day by gavage once a day from gestational day (GD) 5 to 18. On GD19 or GD22/postnatal day one (PD1), the dams were euthanized, and the changes in organ weights and thymus phenotypes were examined for their offsprings. At 750 mg DBP/kg$\cdot$b.w./day in maternal exposure group, GD19 fetuses showed decreases in body weight. The spleen/body weight ratios were reduced in GD 19 fetuses from the dams exposed to 500 and 750 mg DBP/kg$\cdot$b.w./day. There were no significant changes in thymus and spleen cellularities though these cellularities showed a tendency to decrease in a dose dependent way. In the DBP-exsposed GD22/PD1 offsprings, the body weights, the relative organ weights and the cellularities did not exhibit alteration. Additionally, the percentages of CD3$^{+}$(CD4$^{+}$CD8$^{+}$, CD4$^{+}$CD8$^{-}$, CD4$^{-}$CD8$^{+}$, and CD4$^{-}$CD8$^{-}$) and CD3$^{-}$(CD4$^{+}$CD8$^{+}$, CD4$^{+}$CD8$^{-}$, CD4$^{-}$CD8$^{+}$, and CD4$^{-}$CD8$^{-}$) thymocyte subsets were not changed in any DBP-treated group. The proliferative responses of splenic T cells to Con A and B cells to LPS were decreased in all DBP-exposed GD22/PD1 offsprings.

• Reproductive and Developmental Biology
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• 제30권4호
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• pp.235-245
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• 2006

Di-n-butyl phthalate (DBP), diisononyl phthalate (DINP) and di-(2-ethylhexyl) adipate (DEHA) are ubiquitously distributed chemicals that are widely used as plasticizers and also found at low levels in foods. The aims of this study were to determine whether perinatal exposure to DBP, DINP and DEHA could alter normal patterns of neonatal development. Dams were provided with pulverized soy-free diet containing 20, 200, 2,000 and 10,000 ppm of DBP, 40, 400, 4.000 and 20,000 ppm of DINP, or 480, 2,400 and 12,000 ppm of DEHA from gestational day 15 to postnatal day 21. Exposure to the high doses of DBP, DINP and DEHA during gestational period significantly decreased food consumption and body weight gain of dams. These chemicals reduced neonatal body weight as well as that of the after maturation. Also, exposure to DINP of all the doses used and the higher doses (2,400 and 12,000 ppm) of DEHA decreased AGD at PND 1 in male neonates, though that to DBP did not affect AGD in males. In female neonates, an increase in AGD was observed in DBP- and DINP-exposed animals at the highest doses. Moreover, these chemicals affected survival rate of pups at PND 5, and delayed onset of eye opening in all chemica1-exposed groups at PND 17. These results suggest that perinatal exposure to these chemicals may affect the normal development and/or growth of offspring.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Current Trends in Toxicological Sciences
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• pp.98-98
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• 2002

Di-n-butyl phthalate(DBP) is one of the most commonly used phthalic acid esters(PAEs). It is extensively used as a plasticizer in elastomers and explosives, as a solvent for printing inks and resins and as a textile lubricating agent. It is also present in the formulations of various cosmetic products.(omitted)

• 대한화학회지
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• 제19권4호
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• pp.269-279
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• 1975

合成한 pllyglyceryl phthalate, bisphenol A-epichlorohydrin 축중합물과 polyvinyl alcohol의 naphthoquinone-1,2-diazide-5-sulfonyl esters(PGND, BEND 및 PVAND)의 感光特性을 지배하는 諸因子를 이들에 대한 光照射前後의 溶解度 차이에 의하여 檢討하였다. 유리 또는 石英支持薄板上에 塗布한 各試料를 各種條件下에서 露光하고 알카리 水溶液에 침지시킨 다음 塗布된 필림의 殘膜收率(W/W0)을 計算하였다. 필름의 感光度와 직접적인 관계가 있는 殘膜收率은 母體樹脂의 重合度, 增感劑의 種類 및 添加濃度에 따라 영향이 있었다. 母體樹脂의 重合度가 크면 클수록 感光性이 더 우수하였으며, 增感劑의 添加濃度는 5%일때가 가장 좋은 效果를 나타내었다. PGND, BEND 및 PVAND에 대해 본실험에서 사용한 各種 增感制 중에서 가장 效果的인 것은 benzanthrone, 5-nitroacenaphthene, picramide이였다. 이들 樹脂와 增感劑를 添加한 경우의 固相에 있어서의 紫外線吸收스펙트럼과 感色性 寫眞을 각각 비교 검토하여 그 增感과 分光感度的 特性을 糾明하였다. PGND, BEND 및 PVAND는 使用한 增感制에 의하여 光學增感하였으며 增感效果가 현저한 것일수록 그 感光波長城이 長波長쪽으로 伸張하였다. 未增感, 增感試料에 있어서 分光吸收 極大 波長城과 分光感光極大波長城이 거의 一致하고 있는 점으로 보아 試料 樹脂들에 의해서 吸收된 波長城의 光은 그 分野에 有效하게 使用되고 있음을 확인하였다

• 분석과학
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• 제13권5호
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• pp.616-623
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• 2000

Phthalates 8종 및 adipate에 대해 수질 및 저질중의 함유량을 동시에 측정하였다. 수질의 경우는 n-헥산을 사용하여 액체-액체 추출법으로 추출하였고 농축시킨 후 n-헥산에 용해시켜서 분석하였으며 저질의 경우에는 디클로로메탄을 추출용매로 사용하여 초음파세정기를 이용하여 추출한 후 농축시켜서 이소옥탄에 용해시켜서 GC/MS에 주입하여 SIM 방법으로 분석하였다. 그 결과 0.1-20 ng/ml(수질)과 10-500 ng/g(저질, DEP: 10-250 ng/g; DEHP: 10-2000 ng/g)의 정량구간내에서 $R^2=0.993$ 이상의 좋은 직선성을 나타내었으며 검출한계는 0.1 ng/ml(수질)과 10 ng/g(저질) 이하이있다. 본 분석방법은 낮은 농도에서도 정밀도와 정확도가 양호하였다.

• 한국응용과학기술학회지
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• 제24권3호
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• pp.211-218
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• 2007

Based on the Benzoflex (Vesicol Chemical Co.) as PVC plasticizer substituents for Di-n-octyl phthalate (DOP), a series of new aromatic carboxylic acid ester compounds were designed as plasticizers, synthesized, and screened for the endocrine disrupting activity. 2-Hydroxybenzoic acid (1) and 2-methoxybenzoic acid (2) as the commercially available starting materials were reacted with diethylene glycol (3) in the presence of p-toluenesulfonic acid using Dean-Stark column to give diethylene glycol di-(2-hydroxy)benzoate (4, KH01) and diethylene glycol di-(2-methoxy)benzoate (5, KH02), respectively. And diethylene glycol di-(3-pyridinyl) ester (7, KH03) and dipropylene glycol di-(3-pyridinyl) ester (9, KH04) were obtained in high yields by treatment of nicotinoyl chloride (6) with diethylene glycol (3) and dipropylene glycol (8) in the presence of triethylamine as a base. To determine the estrogenic disrupting effect of new synthetic phthalate analogues, E-screen assay method was used. Of these compounds, 4 (KH01) was found to be compound without endocrine disrupting effect.

• 한국환경과학회지
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• 제14권6호
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• pp.597-603
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• 2005

In this study, we try to determine the distributive property in southwest coastal waters, such as K wang-yang bay, Ka-mak bay, Yeo-ja bay, Wan-do, Hea-nam, Young-gwang, and Mok-po, using simultaneous analytical method of 310 chemicals. The results were detected tens of the organic pollutants in sampling sites, and the major chemicals detected were CH type chemicals such as aliphatic, polycyclic compounds and were CHN(O) type chemicals such as aromatic amines, nitro compounds, In particular, pesticides were mainly detected in summer, phenols and phthalate esters were not seasonal effect. Also, a number of aromatic chemicals were detected in Kwang-yang bay, From the results of this study, we confirmed that it is mainly contaminated in summer and the pesticide chemicals are the major pollutants in southwest coastal waters.

• 한국대기환경학회지
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• 제25권5호
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• pp.382-391
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• 2009

The aim of the study was to assess the levels in typical central borough of phthalate exposure by monitoring children's facilities (19 kindergartens and 21 elementary schools) and households (17 old apartments and 22 new apartments) via sampling indoor floor dust. A vacuum cleaner specifically prepared for sampling dust was attached to a filter of a vacuum cleaner dust collecting container. During the sample preparation process, containers made of glass were used and analysis was performed using GC-MSD. The mean concentration of DEHP was $412{\mu}g/g$, DnBP was $24{\mu}g/g$, and BBzP was $10{\mu}g/g$ dust. The indoor characteristics including floor, wall materials, years after construction, water leakage history for the past three years, and ventilation were also examined to categorize phthalate esters from the dust samples. From the flooring and wallpaper materials of kindergartens and elementary schools, DEHP in the dust sample appeared at a statistically significant level (p<0.05) when PVC materials were present. DEHP in the indoor dust also increased significantly (p<0.01) during the construction period. The daily intake of DEHP measured from indoor dust was 0.08~$19.7{\mu}g/kg/day$ for children and 0.02~$1.1{\mu}g/kg/day$ for adults (women). The results clearly revealed that phthalate exposure is greater in children than in adults (women) due to the indoor dust.

• Environmental Analysis Health and Toxicology
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• 제28권
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• pp.13.1-13.5
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• 2013

Objectives: Potential environmental risks caused by chemicals that could be released from a recycled plastic product were assessed using a screening risk assessment procedure for chemicals in recycled products. Methods: Plastic slope protection blocks manufactured from recycled plastics were chosen as model recycled products. Ecological risks caused by four model chemicals - di-(2-ethylhexyl) phthalate (DEHP), diisononyl phthalate (DINP), cadmium (Cd), and lead (Pb)-were assessed. Two exposure models were built for soil below the block and a hypothetic stream receiving runoff water. Based on the predicted no-effect concentrations for the selected chemicals and exposure scenarios, the allowable leaching rates from and the allowable contents in the recycled plastic blocks were also derived. Results: Environmental risks posed by slope protection blocks were much higher in the soil compartment than in the hypothetic stream. The allowable concentrations in leachate were $1.0{\times}10^{-4}$, $1.2{\times}10^{-5}$, $9.5{\times}10^{-3}$, and $5.3{\times}10^{-3}mg/L$ for DEHP, DINP, Cd, and Pb, respectively. The allowable contents in the recycled products were $5.2{\times}10^{-3}$, $6.0{\times}10^{-4}$, $5.0{\times}10^{-1}$, and $2.7{\times}10^{-1}mg/kg$ for DEHP, DINP, Cd, and Pb, respectively. Conclusions: A systematic ecological risk assessment approach for slope protection blocks would be useful for regulatory decisions for setting the allowable emission rates of chemical contaminants, although the method needs refinement.