• 한국물환경학회지
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• 제37권6호
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• pp.449-457
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• 2021

Photosynthesis and respiration rate of microalgae are important factors during advanced wastewater treatment research using microalgae, There are several equipments and measurement methods for measuring photosynthesis and respiration, with different challenges that occur during pretreatment and stabilization of the analysis process. Therefore, in this study, for accurate Photosynthesis and Respiration (P&R) analyzer measurement, the analysis process was divided into pre-processing, DO stabilization, and analysis stages and each was optimized to enable accurate evaluation. For this purpose, the effect of DO saturation of the sample on P&R analysis, DO stabilization according to the degassing flow rate, and photoinhibition of the OD level on photosynthesis was investigated. Based on our study results, when DO was supersaturated, photosynthetic efficiency decreased due to photorespiration, making it inappropriate as a P&R sample. In addition, 0.5 L-N2/min level was the optimal nitrogen degassing flow rate for DO desaturation. The inhibition of photosynthetic efficiency by self-shading caused by the increase in OD was observed from OD 2.0, and it was found that P& R analysis is preferably performed on samples with OD less than 2.0. In addition, based on the above three optimization results, an optimized P&R Analyzer instruction for accurate P&R analysis was also presented.

• 한국농림기상학회지
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• 제7권4호
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• pp.296-302
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• 2005

건조 스트레스에 의한 광합성 기구의 피해 양상을 파악하기 위하여 사시나무를 대상으로 광합성 특성의 변화를 조사하였다. 사시나무의 뿌리를 채취하여 근 맹아를 유도하고 각 개체를 증식한 후 실험에 사용하였다. 건조처리는 PEG농도를 무처리구인 대조구와 $0\%,\;2\%,\;5\%,\;and\;10\%$ 처리구로 구분하여, 주1회 300ml씩 관수하여 4주 후 광합성 특성과 광화학계 및 탄소고정계를 분석하였다 수분스트레스의 강도가 증가함에 따라 사시나무의 광합성속도, 기공전도도 및 증산속도는 모두 감소하는 경향을 보였다. 광화학계에서 PEG 처리농도의 증가는 PSII의 양자수율을 감소시켰으며, 이러한 전자에너지 전달의 감소는 순양자수율도 감소시켰다. 한편 호흡속도는 PEG 처리 농도가 증가함에 따라 감소하였으나, 탄소고정계의 광호흡속도는 반대로 증가하였다. 결론적으로 사시나무는 건조스트레스를 받으면 광합성관련 기구가 매우 민감하게 반응하며, 그들의 민감성은 수분스트레스의 강도에 좌우된다. 한편 사시나무는 수분이용효율의 증가와 같은 수분스트레스에 대한 내성 조절 능력을 보여준다.

• 한국가시화정보학회지
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• 제14권1호
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• pp.51-56
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• 2016

Oxygen is a key factor in aerobic reactions and most biological activities. Visualization of oxygen distribution of a chemical process or biological system has been a very challenging object despite of its significance and potential impact. To monitor and visualize the spatial distribution of oxygen concentration, various techniques such as electro-chemical probe, polarographic electrode, LIF(laser-induced fluorescence) and so on have been introduced. Oxygen planar optode which utilizes the oxygen quenching of fluorescence light is one of the currently available methods for time-resolved visualization of oxygen distribution on a planar surface. In this study, we utilized VisiSens oxygen planar optode system to visualize the spatial distribution of oxygen concentration on leaves of Korean azalea. As a result, temporal variation of oxygen concentration distribution caused by respiratory activity of the leaf could be quantitatively monitored.

• Journal of Ecology and Environment
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• 제29권1호
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• pp.41-47
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• 2006

We investigated the enect of $O_3$ on the photosynthetic characteristics of tree of heaven (Ailanthus altissima) that is naturalized plant and used as restoration plant for contaminated area. Two-year-old seedlings were planted to pots and transferred into closed $O_3$ chamber. Photosynthetic pigments contents and photosynthetic characteristics were measured every three weeks under 100 pub $O_3$ fumigation. There was no visible foliar injury by $O_3$ exposure and contents of photosynthetic pigments did not show significant differences between control and $O_3$-treated seedlings. Also there were no significant differences in stomatal conductance, and water use efficiency. But photosynthetic rate and apparent quantum yield (AQY) of $O_3$ treated seedlings were reduced after nine weeks of ozone fumigation. In addition, the reduction of carboxylation efficiency and photorespiration were observed in the leave of $O_3$ treated seedlings after six weeks. In accordance with our result, carbon fixation system of A. altissima was most sensitive to $O_3$ stress to evaluate physiological damage induced by $O_3$.

• 한국토양비료학회지
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• 제6권4호
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• pp.239-244
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• 1973

대두(大豆)의 안산(燐酸) 감수성(感受性)과 관련(關聯) 엽(葉)의 광합성(光合性)-호흡평형점(呼吸平衡點)과 유리(遊離) 아미노산에 대(對)한 질소원(窒素源)의 영향(影響)을 조사(調査)하였다. 유리(遊離) 아미노산 함량(含量)은 암모니움 태(態)에서 최고(最高)였고 요소태(尿素態)에서 최저(最低)였다. glycine, serine, alanine과 특히 histidine이 암모니움태(態)에서 높았다. aspartic acid 는 초산태(硝酸態)에서 높았다. 광합성(光合性)-호흡평형점(呼吸平衡點)은 감수성(感受性) 품종(品種)에서 높고 초산태(硝酸態)에서 보다 암모니움태(態)에서 높았다. 감수성(感受性) 품종(品種)에서 과잉흡수(過剩吸收)된 암모니움을 광합성(光合性) 회로(回路)에서 중간대사물(中間代謝物)을 탈취(脫取)하여 조해(阻害)하고 오탄당(五炭糖) 회로(回路)와 광호흡회로(光呼吸回路)가 활발(活發)해지며 인산감수성품종(燐酸感受性品種)에서는 이러한 현상이 더욱 조장(助長)되는 것으로 나타났다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.126-126
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• 2017

Mitochondria are important in wheat, as in all crops, as the main source of ATP for cell maintenance and growth including vitamin synthesis, amino acid metabolism and photorespiration. To investigate the mitochondrial proteome of the roots of wheat seedlings, a systematic and targeted analysis were carried out on the mitochondrial proteome from 15 day-old wheat seedling root material. Mitochondria were isolated by Percoll gradient centrifugation; and extracted proteins were separated and analyzed by Tricine SDS-PAGE along with LTQ-FTICR mass spectrometry. From the isolated the sample, 184 proteins were identified which is composed of 140 proteins as mitochondria and 44 proteins as other subcellular proteins that are predicted by the freeware subcellular predictor. The identified proteins in mitochondria were functionally classified into 12 classes using the ProtFun 2.2 server based on biological processes. Proteins were shown to be involved in amino acid biosynthesis (17.1%), biosynthesis of cofactors (6.4%), cell envelope (11.4%), central intermediary metabolism (10%), energy metabolism (20%), fatty acid metabolism (0.7%), purines and pyrimidines (5.7%), regulatory functions (0.7%), replication and transcription (1.4%), translation (22.1%), transport and binding (1.4%), and unknown (2.8%). These results indicate that many of the protein components present and functions of identifying proteins are common to other profiles of mitochondrial proteins performed to date. This dataset provides the first extensive picture, to our knowledge, of mitochondrial proteins from wheat roots. Future research is required on quantitative analysis of the wheat mitochondrial proteomes at the spatial and developmental level.

• Journal of Ginseng Research
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• 제13권1호
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• pp.98-104
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• 1989

In order to determine the specific active oxygen species directly related to chlorophyll bleaching in the leaf-burning disease, we investigated the effects of singlet oxygen (1O2), superoxide radical (O2-), and hydrogen Peroxide (H2O2) on isolated chloroplast suspension and leaf discs from Panax ginseng C.A. Meyer. When the singlet oxygen was added to the chloroplast suspension, the chlorophyll and carotenoid contents were decreased by more than 809), similar to treatment with high light intensity (100 KLux). We assumed that the conversion of dioxygen (O2) produced either in photolysis or in breakdown of hydrogen peroxide to singlet oxygen resulted from photorespiration. On the basis of these experiments , :he inhibitory effects of active oxygen scavengers propylgallic acid (PGA), 2,5-ditetrabutyl hydroquinon (DBH), sodium pyrosulfate (SPS), and ascorbic acid (ABS) were examined. In chloroplast suspension all four scavengers inhibited chlorophyll bleaching by more than 75fl , and in the leaf discs the inhibition rates of SPS, PGA and ABS were 46%, 51%, and 96% respectively.

• Journal of Ginseng Research
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• 제33권4호
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• pp.249-255
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• 2009

The oxidation of glycolate to glyoxylate, a key step in plant photorespiration, is carried out by the peroxisomal flavoprotein glycolate oxidase (EC 1.1.3.15). To investigate the altered gene expression and the role of GOX in ginseng plant defense system, a cDNA clone containing a GOX gene designated as PgGOX was isolated and sequenced from Panax ginseng. The cDNA was 692 nucleotides long and have an open reading frame of 552 bp with a deduced amino acid sequence of 183 residues. A GenBank BlastX search revealed that the deduced amino acid of PgGOX shares a high degree homology with the Glycine max (95% identity). In the present study we analyzed the expression of PgGOX under various environmental stresses at different times using real time-PCR. The results showed that the expressions of PgGOX increased after various treatments involving salt, light, cold, ABA, SA, and copper treatment.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1980년도 학술대회지
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• pp.151-170
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• 1980

Physiological response of Panax ginseng var. atropurpureacaulo (purple stem variety, Pg) to light was reviewed through old literatures and recent experiments. Canopy structure, growth, pigment, leaf anatomy, disease occurence, transpiration, photosynthesis (PS), leaf saponin, photoperiodism and nutrient uptake were concerned. P. ginseng var. xanthocarpus (yellow berry variety, Px) and Panax quinquefolius(Pq) were compared with Pg if possible. Compensation point(Cp) increased with increase of light and ranged from 110 to 150 at $20^{\circ}C$ but from 140 to 220 at $30^{\circ}C$ with 4 to 15 Klux indicating occurence of light and temperature-dependent high photorespiration. Characteristics of Korea ginseng to hate high temperature was well accordance with an observation 2000 years ago. Korea ginseng showed lower Cp and appeared to be more tolerant to high light intensity and temperature than American sheng although the latter showed greater PS, stomata frequency and conductance, chlorophyll and carotenoids. Px showed lower PS than Pg probably due to higher Cp. Total leaf saponin was higher in leaves grown under high light. Ratio or diol saponin and triol saponin(PT/PD) decreased with increase of light intensity during growing mainly due to decrease of ginsenoside $Rg_1$ but increase of ginsenoside Rd. Leaves of Pg and Px had $Rg_1$ but no $Rb_3$ which was only found as much as $20\%$ of total in Pq leaves, and decreased with increase of light intensity. Re increased in Pg and Px but decreased in Pq with increase of light. PT/PD in leaf ranged 1.0-1.5 in Pg and Px but around 0.5 in Pq. Korea ginseng has Yang characteristics(tolerant to high light and temperature), cultured under Eum(shade) condition and long been used for Yang efficacy (to build up energy) while Pq was quite contrary. Traditional low light $intensity(3-8\%)$ for Korea ginseng culture appeared to be strongly related to historical unique quality. Effect of light quality and photoperiodism was not well known. Experiences are long but scientific knowledge is short for production and quality assessment of ginseng. Recent scientific knowledge of ginseng should learn wisdom from old experiences.

• 한국약용작물학회지
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• 제3권3호
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• pp.181-186
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• 1995

본(本) 연구(硏究)는 고추냉이의 광합성(光合成) 효율증진(效率增進)을 위한 기상환경(氣象環境)에 조건(條件)을 알아내어 재탑적지(栽培適地)의 선정(選定)과 재배관리(栽培管理)에 대한 기초적(基礎的)인 자료(資料)를 제공(提供)하고자 온도(提供)와 광도(光度), 그리고 $CO_2$의 농도(農度)에 대한 광합성(光合成), 청호흡(晴呼吸) 그리고 광호흡(光呼吸)의 반응(反應)을 조사(調査)하였다. 1. 고추냉이의 광합성(光合成)에 최적온도(最適溫度)는 $17{\sim}20^{\circ}C$이었고 최적온도(最適溫度) 이상(以上)에서 광합성(光合成)이 크게 감소(減少)하였으며, 암호흡(暗呼吸)은 $15^{\circ}C$에서 $30^{\circ}C$까지 온도(溫度)가 높아질수록 증가(贈加)하였다. 2. 광보상점(光補償點)은 고추냉이가 $6.0 {\mu}E\;m^{-2}s^{-1}$으로 옥수수의 $36.7{\mu}E\;m^{-2}s^{-1}$보다 낮았으며 광포화점(光飽和點)은 고추냉이와 옥수수 모두 $600{\mu}E\;m^{-2}s^{-1}$로 비슷하였다. 3. $CO_2$ 보상점(補償點)은 고추냉이가 67. 3 ppm으로 옥수수 11.6 ppm보다 높았으며 암호흡(暗呼吸)은 $CO_2$의 농도(濃度)가 낮을수록 높았다. 4. 고추냉이의 광호흡(光呼吸)은 광도(光度) $l000{\mu}E\;m^{-2}s^{-1}$에서 $3.3 mg\;CO_2\;dm^{-2}\;hr^{-1}$이었으며 광도(光度)가 낮아짐에 따라 감소(減少)하였다. 5. 기공(氣孔)의 분포(分布)는 잎의 표면(表面)에 약 76개/$mm^{-2}$, 뒷면에 623개/$mm^{-2}$이었으며 기공(氣孔)의 크기는 표면(表面)의 것이 $12{\mu}m$이고 뒷면의 것이 $17{\mu}$로 나타났다.