• 자연과학논문집
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• 제11권1호
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• pp.65-73
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• 1999

C-P 화합물(Pn; phosphonate)의 일종인 glyphosate(GPS)를 인산원으로 이용하는 Pseudomonas sp. strain #A1으로부터 GPS 분해 유전자 및 2-aminoethylphosphonate(AEPn), methyl-phosphonate(MPn)와 같은 Pn의 분해 유전자를 클로닝하였다. Mini-Mu plasmid를 이용한 in vivo molecular cloning 결과 약 10-19 Kb의 $AEPn^+$ clones, 10 Kb의 $MPn^+$ clones, 12-18 Kb의 $GPS^+$ clone들을 얻었으며 E. coli의 $\Delta phn$ mutants에 transformation 하였을 때 각각의 Pn에 대한 다양한 phenotype을 나타냈다. 따라서 Pseudomonas sp. strain #A1에서는 적어도 3종류의 Pn 분해대사 경로를 갖고 있는 것으로 예측된다. 뿐만 아니라, 각각의 phn clone($AEPn^+$, $MPn^+$, $GPS^+$)들은 PHO regulon의 조절유전자 phoBR에 의존하여 발현하였다.

• Journal of Microbiology
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• 제33권4호
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• pp.328-333
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• 1995

$\^$31/ P-NMR experiment was performed to detect phophonates (Pn) utilization and degradation in the several different C-P lyase mutants of E. coli and in E. aerogenes and the recombinants. The relative peak intensity (RPI) for the standard samples of 0.5 mM methylphosphonate (MPn) and 1.0 mM aminoethylphosphonate in glucose-MOPS medium showed 0.5 : 1.0 ratio. In the case of BW14329 (.DELTA.phnC-P, .delta.phoA), RPI did not change significantly after 24 hrs culturing, which means it nearly could not utilize Pn. In vivo $\^$31/ P-NMR spectrum of E. aerogens (BWKL 16627) during 3 hrs starvation showed two intense peaks at 0-2 ppm and at near-10 ppm which indicate intracellular orthophosphate (Pi) and pyrophosphate (PPi), respectively. Both of them might be released by degradation of inorganic polyphosphate pool. When MPn is supplied to the medium as an unique P source, Pi content in the cell has the constant, but PPi seems to be slightly decreased. Recombinants (BWKL 16954) grew slower than E. aerogenes in the glucose-MOPS media with various P sources. In vivo $\^$31/ P-NMR spectrum of recombinant did not show any intense signal in the cell. Surprisingly, under the cultivation adding with MPn, a few intense peaks in the region of Pi AND phospate monoester were detected.