• 한국식품영양학회지
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• 제3권1호
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• pp.39-56
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• 1990

The effects of six kinds of phosphate complex on the water holding capacity (W.H.C) and protein solubility of hair tail, yellow tail runner and dried pollack meat paste were investigated and animal meat(pork, chicken and hare meat complex) The formulation of six kind of phosphate complex employed to this experiment were made by mixing several phosphate such as sodium polyphosphate, sodium pyrophosphate, sodium acid pyrophosphate, potassim pyrophosphate, sodium ultra-meta-phosphate, sodium-tetra-phosphate and monoglyceride at different mixture ratio Among the six kinds of phosphate complex, phosphate B complex which was formulated by mixing sodium polyphosphate 40%, sodium pyrophosphate 30%, sodium tetra mata phosphate 10%, sodium ultra meta phosphate 10% was most effective on enchanging the W H. C, and protein solubility of hair tail, yellow tail runner dried pollack meat past and in case of pork, chicken and hare meat paste. Phosphate C complex which was formulated by mixing sodium polyphosphate 50%. sodium pyrophosphate 30%, sodium tetra meta phosphate 10%, potassium pyrophosphate 10%, was more effective them other phosphate complex, and thief optimum addition level was 0.5% respectively in weight of fish meat paste. Texture characteristics such as hardness, cohesiveness and springiness value of Kamaboko(fish meat and pork, chicken, hare meat complex past meat product) were evaluted as best when 0.5% of Phosphate B complex was added The optimum cooking condition of Kamaboko to get good texture was heating for 20 minutes at 12$0^{\circ}C$.

• 한국식품영양학회지
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• 제10권4호
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• pp.544-548
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• 1997

인산염의 첨가가 조기 및 갈치연육의 보수력 및 단백질의 용출성에 미치는 영향과 가열처리 후에 조기육을 원료로 가공한 어묵의 texture에 미치는 영향을 조사하기 위하여 인산염을 첨가하지 않은 대조구와 4종의 시판 복합 인산염을 첨가수준별로 사용했을 때의 기능적 성질을 분석 검토하였다. 연육의 보수력 및 염용성 단백질량은 조기연육에는 인산염 B(sodium pol-phosphate 50%, sodium pyro-phosphate 20%, sodium tetra meta-phosphate 20%, sodium acid pyro-phosphate 5%, sodium ultra meta-phosphate 5%)를, 갈치연육에는 인산염 C(sodium poly-phosphate 40%, sodium pyro-phosphate 30%, potassium pyro-phosphate 15%, sodium tetra meta-phosphate 10%, hexa meta-phosphate 5%)를 각각 0.4% 첨가한 경우가 가장 효과적이었고 그 때의 염용성 단백질의 용출량은 조기연육이 954mg%, 갈치연육이 686mg%였다. 조기육을 원료로 하여 가공한 어묵의 적정 가열조건은 85$^{\circ}C$에서 45분간 가열한 것이 조직감이 양호하였다. 한편 121$^{\circ}C$에서 10분간 가열한 경우는 C 인산염이 다른 인산염에 비하여 어육 단백질의 열안 정성이 높은 것으로 나타났다.

• 한국식품과학회지
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• 제17권4호
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• pp.253-257
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• 1985

인산염의 첨가가 조기 및 갈치연육의 보수력 및 단백질의 용출성에 미치는 영향과 가열처리 후에 조기육을 원료로 가공한 어묵의 texture에 미치는 영향을 조사하기 위하여 인산염을 첨가하지 않은 대조구와 4종의 시판 복합 인산염을 첨가수준별로 사용 했을때의 기능적 성질을 분석 검토 하였다. 연육의 보수력 및 염용성 단백질량은 조기연육에는 인산염B(sodium poly-phosphate 50%. sodium Poly-Phosphate20%, sodium tetra meta-Phosphate 20%, sodium acid pyro-phosphates%, sodium ultra meta-phosphate 5%)를 갈치연육에는 인산염C(sodium poly-phosphate40%, sodium pyro-phosphate 30%, potassium pyro-phosphate 15%, sodium tetra meta-phosphate10%, sodium hexa meta-phosphates%)를 각각 0.4% 첨가한 경우가 가장효과적 이였고 그때의 염용성 단백질의 용출량은 조기연육이 954mg%, 갈치연육이 686mg%였다. 조기육을 원료로 하여 가공한 어묵의 적정가열 조건은 $85^{\circ}C$에서 45분간 가열한 것이 조직감이 양호 하였다. 한편 $121^{\circ}C$에서 10분간 가열한 경우는 C인산염이 다른 인산염에 비하여 어육 단백질의 열 안정성이 높은 것으로 나타났다.

• Journal of Applied Biological Chemistry
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• 제49권3호
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• pp.86-89
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• 2006

The novel tetranuclear nickel (II) complex is a high rate accelerator in promoting hydrolysis of phosphate diesters. Nickel-bound bis-nitrophenyl phosphate (BNPP) can be $10^4$ times more reactive than the unbound BNPP. The large rate of enhancements by the complex slightly under basic condition has shown high catalytic activity in phosphate diester cleavage. The bell-shaped pH-rate profile indicated that the nickel-oxide form of the tetranuclear complex or its kinetic equivalent was the active species for cleaving BNPP. The catalytic hydrolysis between tetranuclear nickel (II) complex and phosphate diester proceeds via the formation of bidentate coordination of the anionic phosphate to the Ni (II) atom. This reveals that the complex has the possibility as artificial nuclease.

• Molecules and Cells
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• 제27권1호
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• pp.99-103
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• 2009

Ribose-5-phosphate isomerase A (RpiA) plays an important role in interconverting between ribose-5-phosphate (R5P) and ribulose-5-phosphate in the pentose phosphate pathway and the Calvin cycle. We have determined the crystal structures of the open form RpiA from Vibrio vulnificus YJ106 (VvRpiA) in complex with the R5P and the closed form with arabinose-5-phosphate (A5P) in parallel with the apo VvRpiA at $2.0{\AA}$ resolution. VvRpiA is highly similar to Escherichia coli RpiA, and the VvRpiA-R5P complex strongly resembles the E. coli RpiA-A5P complex. Interestingly, unlike the E. coli RpiA-A5P complex, the position of A5P in the VvRpiA-A5P complex reveals a different position than the R5P binding mode. VvRpiA-A5P has a sugar ring inside the binding pocket and a phosphate group outside the binding pocket: By contrast, the sugar ring of A5P interacts with the Asp4, Lys7, Ser30, Asp118, and Lys121 residues; the phosphate group of A5P interacts with two water molecules, W51 and W82.

• 한국식품과학회지
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• 제19권1호
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• pp.75-80
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• 1987

마늘 플레이크 건조시 갈변을 방지하기위하여 축합 인산염의 농도 및 침지시간에 따라서 마늘 플레이크의 갈변현상을 조사하였다. 마늘 플레이크 건조중 갈변억제는 인산염 농도, 침지시간 및 환원당에 의해서 크게 좌우되며 갈변물질의 형성속도는 환원당 함량과 반비례의 관계가 있었다. 인산염 처리는 0.3%에서 3분간 침지함으로서 마늘 건조중 갈변을 억제할 수 있었으며 pyruvate 성분의 감소는 $0.2{\sim}0.3%$에서 3분 침지하는 것이 가장 적었다. 건조수율을 인산염을 처리하면 처리하지 않을 경우보다 $1.62{\sim}1.75%$의 수율이 떨어졌는데 이는 가용성 물질이 용출되어 수율이 감소되는 것으로 생각되었다.

• 미생물학회지
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• 제28권2호
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• pp.134-144
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• 1990

In order to examine that what kind of system correlated with cadmium detoxification mechanism in Klebsiella aerogenes ATCC 10031, we tried to investigate the effect of phosphate upon the detoxification and also elucidate whether the cadmium phosphate and/or polymeric Cd-Pi complex is formed actually in cell or not. As the results, it was shown that growing pattern had long lag adaptive phase of 12 hr to 24 hr, at the concentrations of 0.02 mM and 0.08 mM cadmium, respectively. Cadmium was accumulated more highly in the fraction of cell wall and membrane than in those of cytoplasm. In case of phosphate starving cells added cadmium, inorganic polyphosphate system was primarily correlated with Cd-detoxification during the lag phase for the accommodation to cadmium, on the other hand, Cd:Sulfide complex system secondarily correlated it during the stationary phase. These results implied that polyphosphate system and Cd:sulfide complex system, these two systems were operated compensatively each other. Considering the results obsdrved with EM and examined tha changes of sulfide and polyphosphate amount, it was reflected that Cd:S complex was located at the cell surface. In the results of $in-vivo^{31}$P NMR spectra in the cells with cadmium pressure, several phosphate signals arose newly from the polyphosphate region with moving chemical shift of it. This phinomenon strongly implied the actual existence of Dd:Pi comples and /or Cd:poly-P complex in the cell and also the cellular compartmentalization of cadmium detoxifying mechanism.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2005년도 춘계학술발표회
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• pp.311-312
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• 2005

The precipitation of U(VI) in the presence of phosphate and carbonate was investigated in the pH range of 4 to 13 and the following was obtained as a result of this experimental condition. U(VI) precipitates as a $NaUO_{2}PO_{4}$ at pH<9 but as mixtures of phosphate, hydroxides and/or carbonate at pH>9. The portion of the phosphate in the precipitate decreases almost linearly to near zero with an increasing pH in the range of 9 to 13. The U(VI) phosphate is dissolved by the carbonate complex formation at pH<10.5. The ternary complex of a carbonate and phosphate is not found.

• Bulletin of the Korean Chemical Society
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• 제22권2호
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• pp.199-204
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• 2001

A Cryptate (221) having a short alcohol pendant (2) was metallated with europium(Ⅲ) in anhydrous condition, and its hydrolytic activity for phosphate esters at neutral pH was examined. While the activity for the phosphate diester and monoester is comparable to that of the parent metal complex [1Eu]3+, its hydrolytic activity towards a phosphate triester is significantly suppressed. Potentiometric titration and luminescence spectroscopic studies for the equilibrium behavior of the complex in solution suggest that a dimer formation through the metal hydroxides as well as the pendant alcohol is likely to happen. The low hydrolytic activity for the triester seems to be associated with the dimer formation.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.415-419
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• 1994

A simple nad rapid colorimetric method for the assay of amodiaquine hydrochloride, chloroquine phosphate and primaquine phosphate is described. The method is based on the interaction of the drug base with bromophenol blue to give a ion-pair complex. The spectra of the complex shows a maxima at 415-420 nm with high apparent molar absorptivities. Beer's law was obeyed in the concentration range 1-8, 2-10 and $2-12{\;}{\mu}{\cdot}ml^{-1}$ for amodiaquine hydrochloride, primaquine phosphate and chloroquine phosphate respectively. The proposed method was applied to the determination of these drugs in certain formulations and the results were favourably comparable to the official methods.