• 한국자원식물학회지
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• 제12권3호
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• pp.198-203
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• 1999

콩 저장 단백질의 대부분은 globulin이며, 이중 7S와 11S가 70% 이상을 차지한다. 따라서 콩 단백질의 조성개량을 위해서는 11S/7S비율 조정이 우선되는데, 본 연구에서는 전기영동(SDS-PAGE)법을 사용하여 콩 단백질 7S와 11S를 분리 확인하고, 이들 분획 단백질의 유전변이를 분석하였다. 국내 3개지역에서 재배된 콩 장려품종 6계통들의 평균 7S 함량은 38.9% 이었고 11S는 61.2%의 함량을 나타내었다. 분산분석 결과 품종간에 는 유의성이 있었지만 지역간에는 변이가 없었으며, 품종 x 지역의 상호작용은 고도의 유의성을 나타내었다. 유전력은 7S분획중의 $\beta$함량이 72.7%로 높게 나타났다. 공분산을 이용한 상관계수 추정에서는 유전상관이 표현형 상관 보다 다소 높게 나타났다. 따라서 7S와 11S의 분획간 함량을 조정함으로써 콩 단백질의 조성을 개량할 수 있으리라 판단된다.

• 대한의생명과학회지
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• 제25권1호
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• pp.75-82
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• 2019

We developed the multiplex LAMP assay using 16S rRNA, femA and mecA genes for direct detection of the methicillin resistance in Staphylococci from positive blood culture. To simultaneously recognize Staphylococci genus, S. aureus and methicillin resistance, three sets of six primers for 16S rRNA, femA and mecA were designed, respectively. The performance of LAMP assay was affirmed using VITEK system for the phenotypic methods of identification and for oxacillin and cefoxitin antimicrobial susceptibility. The optimal condition for LAMP assay was obtained under $64^{\circ}C$ for 50 min. The detection limit was determined to be of 20 copies and CFU/reaction ($10^4CFU/mL$). For clinical application of comparison with phenotypic methods, the sensitivity and specificity of the LAMP with femA gene for detecting S. aureus was 95.31% and 100%, respectively. The sensitivity and specificity of the LAMP with mecA gene for detecting methicillin resistance was 98.46% and 100%, respectively. The multiplex LAMP assay with femA and mecA gene successfully detected all of MRSA (38 isolates) isolates from 103 Staphylococci in blood cultures. The LAMP assay developed in this study is sensitive, specific, and of excellent agreement with the phenotypic methods.

• Tuberculosis and Respiratory Diseases
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• 제82권2호
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• pp.143-150
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• 2019

Background: The purpose of this study was to analyze the relationship between the gene mutation patterns by the GenoType MTBDRplus (MTBDRplus) assay and the phenotypic drug susceptibility test (pDST) results of isoniazid (INH) and prothionamide (Pto). Methods: A total of 206 patients whose MTBDRplus assay results revealed katG or inhA mutations were enrolled in the study. The pDST results were compared to mutation patterns on the MTBDRplus assay. Results: The katG and inhA mutations were identified in 68.0% and 35.0% of patients, respectively. Among the 134 isolated katG mutations, three (2.2%), 127 (94.8%) and 11 (8.2%) were phenotypically resistant to low-level INH, high-level INH, and Pto, respectively. Among the 66 isolated inhA mutations, 34 (51.5%), 18 (27.3%) and 21 (31.8%) were phenotypically resistant to low-level INH, high-level INH, and Pto, respectively. Of the 34 phenotypic Pto resistant isolates, 21 (61.8%), 11 (32.4%), and two (5.9%) had inhA, katG, and both gene mutations. Conclusion: It is noted that Pto may still be selected as one of the appropriate multidrug-resistant tuberculosis regimen, although inhA mutation is detected by the MTBDRplus assay until pDST confirms a Pto resistance. The reporting of detailed mutation patterns of the MTBDRplus assay may be important for clinical practice, rather than simply presenting resistance or susceptibility test results.

• 한국동물생명공학회지
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• 제38권4호
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• pp.268-274
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• 2023

Background: Recently, the single-step genomic best linear unbiased prediction (ssGBLUP) method, which incorporates not only genomic information but also phenotypic information of pedigree, is under study. In this study, we performed a ssGBLUP analysis on a commercial Hanwoo population using phenotypic, genotypic, and pedigree data. Methods: The test population comprised Hanwoo 1,740 heads raised in four regions of Korea, while the reference population used Hanwoo 18,499 heads raised across the country and two-generation pedigree data. Analysis was performed using genotype data generated by the Hanwoo 50 K SNP beadchip. Results: The mean Genome estimated breeding values (GEBVs) estimated using the ssGBLUP methods for carcass weight (CWT), eye muscle area (EMA), back fat thickness (BFT), and marbling score (MS) were 7.348, 1.515, -0.355, and 0.040, respectively, while the accuracy of each trait was 0.749, 0.733, 0.769, and 0.768, respectively. When the correlation analysis between the GEBVs as a result of this study and the actual slaughter performance was confirmed, CWT, EMA, BFT, and MS were reported to be 0.519, 0.435, 0.444, and 0.543, respectively. Conclusions: Our results suggest that the ssGBLUP method enables a more accurate evaluation because it conducts a genetic evaluation of an individual using not only genotype information but also phenotypic information of the pedigree. Individual evaluation using the ssGBLUP method is considered effective for enhancing the genetic ability of farms and enabling accurate and rapid improvements. It is considered that if more pedigree information of reference population is collected for analysis, genetic ability can be evaluated more accurately.

• 생명과학회지
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• 제24권4호
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• pp.361-369
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• 2014

16S rRNA gene PCR법은 환자 검체로부터 병원성 미생물을 검출 및 동정에 사용되어진다. 본 연구는 대량의 임상미생물 진단을 위해 bacterial 16S rRNA 부위 유전자 서열을 이용하여 광대한 범위와 높은 특이도를 가지는 primer을 포함한 PCR법을 개발하였다. 10개 표준 균주 16S rRNA 보존 부위의 유전자 서열을 기반으로 primer set를 구축하였다. 98명 환자 검체에서 임상 미생물을 분리하였다. 98개 균주는 phenotypic 방법을 이용하여 확인하고, 개발된 primer set와 universal primer set를 이용한 PCR법으로 확인하였다. 획득한 PCR 산물은 forward primer, reverse primer, 그리고 자동화 DNA 분석기를 이용하여 각 균주의 16S rRNA 유전자 서열을 분석 및 확인하였다. 본 연구에서 개발된 primer set와 universal primer set의 임상미생물 검출에 대한 효율성을 평가하였고, 또한 phenotypic 방법과 분자생물학적 방법을 비교했다. 분리된 98개 균주를 대상으로 개발된 primer set로 16S rRNA PCR을 진행하여 778 bp 크기의 단일밴드로 증폭 되었음을 확인했다. 총 98개중 94개 균주(95.9%)는 phenotypic 결과와 동일함을 확인했다. 새로 개발된 primer set를 이용한 결과는 universal primer set를 이용한 98개 균주(100%)의 결과와 동일함을 확인하였다. 개발된 16S rRNA gene PCR법은 임상미생물 검출 및 동정에서 신속성, 정확성, 그리고 검사 비용 절감의 장점을 가진다. 개발된 primer set는 병원성 미생물 동정에서 효율성을 확인했다.

• Plant Biotechnology Reports
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• 제5권2호
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• pp.135-146
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• 2011

The objective of the present research was to map QTLs associated with agronomic traits such as days from sowing to flowering, plant height, yield and leaf-related traits in a population of recombinant inbred lines (RILs) of sunflower (Helianthus annuus). Two field experiments were conducted with well-irrigated and partially irrigated conditions in randomized complete block design with three replications. A map with 304 AFLP and 191 SSR markers with a mean density of 1 marker per 3.7 cM was used to identify QTLs related to the studied traits. The difference among RILs was significant for all studied traits in both conditions. Three to seven QTLs were found for each studied trait in both conditions. The percentage of phenotypic variance ($R^2$) explained by QTLs ranged from 4 to 49%. Three to six QTLs were found for each yield-related trait in both conditions. The most important QTL for grain yield per plant on linkage group 13 (GYP-P-13-1) under partial-irrigated condition controls 49% of phenotypic variance ($R^2$). The most important QTL for 1,000-grain weight (TGW-P-11-1) was identified on linkage group 11. Favorable alleles for this QTL come from RHA266. The major QTL for days from sowing to flowering (DSF-P-14-1) were observed on linkage group 14 and explained 38% of the phenotypic variance. The positive alleles for this QTL come from RHA266. The major QTL for HD (HD-P-13-1) was also identified on linkage group 13 and explained 37% of the phenotypic variance. Both parents (PAC2 and RHA266) contributed to QTLs controlling leaf-related traits in both conditions. Common QTL for leaf area at flowering (LAF-P-12-1, LAF-W-12-1) was detected in linkage group 12. The results emphasise the importance of the role of linkage groups 2, 10 and 13 for studied traits. Genomic regions on the linkage groups 9 and 12 are specific for QTLs of leaf-related traits in sunflower.

• 한국작물학회지
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• 제49권6호
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• pp.539-545
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• 2004

M/G RIL 164계통과 그 유전자지도를 이용하여 지역에 따른 벼의 품질과 관련된 양적형질 유전자좌(QTL)를 분석한 결과를 보면 다음과 같다. M/G RIL 164계통의 지역에 따른 단백질함량, 아밀로오스 함량, 지방산함량 및 식미평가치에 있어 빈도분포는 정규분포에 가까운 연속변이를 보였으며, 양친의 범위를 벗어나는 초월분리 현상을 나타내었다. 또한 지역에 따라 품질형질의 분포범위의 폭이 다양하게 나타났으며, 단백질함량은 원주>익산>대구의 순으로, 아밀로오스함량, 지방산함량 및 식미평가치는 대구>익산>원주의 순으로 나타났다. 품질에 관련된 QTLs분석에 있어 단백질함량과 관련하여서는 8개의 QTLs를 확인하였으며, 1번 염색체에서 2개, 3번, 6번, 7번 염색체에서 각각 1개, 8번 염색체에서 3개의 QTLs를 확인할 수 있었으며, 이들 8개의 QTLs가 설명할 수 있는 표현형 변이는 $6.0\~15.2\%$로 나타났다. 아밀로오스함량과 관련하여 6번 염색체에서 1개, 7번 염색체에서 2개의 QTLs를 확인하였다. 3개의 QTLs가 설명할 수 있는 표현형 변이는 $7.3\~24.4\%$로 나타났다. 지방산함량과 관련하여서는 2번과 6번 염색체에서 각각 깨, 3번과 7번 염색체에서 각각 1개의 QTLs를 분석하였으며, 6개의 QTLs로 설명할 수 있는 표현형 변이는 $5.5\~14.0\%$를 보였다. 식미평가치와 관련된 QTLs는 2번과 6번 염색체에서 각각 1개, 7번과 8번 염색체에서 각각 2개의 QTLs가 분석되었으며, 그 6개의 표현형 변이는 $5.5\~10.3\%$로 나타났다.

• 한국가금학회지
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• 제39권1호
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• pp.9-15
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• 2012

The aim of this study was to investigate phenotypic characteristics, morphometric measurements, reproduction and production performances of Aseel chicken of Bangladesh. The dominant feather color of neck/hackles was red in both males (56.14%) and females (54.16%) while the sickle feather color was mostly black in both chickens (71.93% vs. 54.17%). The predominant saddle and breast feather colors were red (40.35%) and black (64.91%), respectively, in male whereas most frequent observed color was pale brown in female (58.33 and 50.0%, respectively). The predominant feather color of wing bow and wing bay was found black (68.42 and 80.70%, respectively) in male but only pale brown color was observed in females (62.5 and 54.17%, respectively) for these two characters. Different phenotypic measurements such as the average shank length and circumference were $12.79{\pm}0.13$ and $7.8{\pm}0.08$ cm, respectively, in male and $10.21{\pm}0.25$ and $5.81{\pm}0.21$ cm, respectively, in female. Keel length was $14.39{\pm}0.19$ cm in male and $10.79{\pm}0.23$ cm in female. The average adult live weight in male was measured $3749.12{\pm}83.44$ g while in female it was $2062.50{\pm}105.26$ g. The age of 1st lay was found to be 28.86 weeks. Total number of eggs laid per year ranged between 24~48, number of clutch/hen/year varied from 2 to 4 and number of eggs/clutch/hen was found to be 10~12. The average live weight of Aseel chicken at 1, 2, 3, 4, 6, 8, 10, 12, 16 and 17 weeks of age were recorded as $31.14{\pm}0.55$, $48.63{\pm}3.99$, $116.57{\pm}5.72$, $138.40{\pm}5.91$, $212.88{\pm}4.82$, $361.00{\pm}9.72$, $577.50{\pm}42.86$, $743.75{\pm}24.65$, $1086.00{\pm}26.02$, $1402.00{\pm}24.54$ and $1432.00{\pm}27.00$ g respectively. Finally, this phenotypic characterization as well as productive and reproductive performances of Aseel chicken will give the baseline information to researcher for further study and for planning any on-ward conservation and implement strategy.

• Asian-Australasian Journal of Animal Sciences
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• 제15권8호
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• pp.1080-1084
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• 2002

Carcass characteristics of 241 crossbred pigs (Korean wild boars ${\times}$ Landrace sows) were analyzed to examine variations in fasted body weight (FASTWT), carcass weight (CARCWT), dressing percentage (DP), back fat thickness (BFT) and longissimus muscle weight (LMW), and to estimate genetic and phenotypic parameters using three different slaughter-end points. Covariates in the least squares full sib model were slaughter age, fasted body weight and back fat thickness of the carcass. Coefficient of variation was highest for BFT followed by LMW, CARCWT, FASTWT and DP in magnitude. Regressions of three covariates on traits were all linear. However, slaughter age was not significant as a linear covariate for five traits while FASTWT was significant for CARCWT and LMW and BFT was significant for all remaining traits. Genetic and phenotypic variation was considerably reduced by regressing FASTWT or BFT in the model. Heritability estimates of FASTWT, CARCWT, DP and BFT were 0.68, 0.61, 0.11 and 0.49, respectively, using slaughter age as covariate (model 1). Those of CARCWT, DP, BFT and LMW were 0.15, 0.15, 0.30 and 0.11, respectively, using FASTWT as covariate (model 2). Heritability estimates of the traits using LMW as covariate (model 3) were similar to the estimates from Model 1 except that the estimate of CARCWT was reduced to 0.39. Genetic or phenotypic correlations among FASTWT, CARCWT and BFT were all positive and moderate to high. Those between BFT and LMW were also positive and low to moderate. However, genetic and phenotypic correlations between DP and CARCWT were positive while those between DP and FASTWT were negative. It was suggested from this study that differences in carcass yield traits be determined using slaughter age or back fat thickness as slaughter-end point and carcass quality traits using fasted body weight as slaughter-end point.

• 한국산림과학회지
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• 제26권1호
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• pp.13-18
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• 1975

본시험(本試驗)은 죽간재적(竹稈材積)에 영향(影響)하는 형질(形質)을 정확(正確)하게 파악(把握)하기 위(爲)하여 계획(計劃)되었다. 그리고 왕대의 수개형질상호간(數個形質相互間)의 표현형상관(表現型相關), 유전상관(遺傳相關) 및 환경상관(環境相關)을 구명(究明)하고져 공분산분석(共分散分析) 되었으며 Dewey and Lu의 편회귀분석법(偏回歸分析法)으로 각형질(各形質)이 죽간재적(竹稈材積)에 미치는 효과(効果) 즉(即) 경로계수(經路係數)를 산출(算出)하였다. 조사형질(調査形質)은 흉고직경(胸高直徑), 죽간고(竹稈高), 지상고(枝上高), 지하경(地下莖), 절수(節數), 근원직경(根元直徑) 및 죽간재적(竹稈材積)이며 각(各) 형질(形質)의 평균치(平均値), 표준오차(標準誤差), 분산(分散) 및 표준편차(標準偏差)는 제(第)1표(表)와 같고 7개형간(個形間)의 표현형(表現型), 유전(遺傳) 및 환경상관(環境相關)을 분산분석(分散分析)한 결과(結果)는 제(第)2표(表)와 같이 계통간(系統間)에는 고도(高度)의 유의성(有意性)이 인정(認定)되었으나 몇몇 반복간(反覆間)에는 유의성(有意性)이 인정(認定)되지 않았다. 각(各) 형질(形質)의 유전력(遺傳力)을 추정(推定)한바 흉고직경(胸高直徑)과 근원직경(根元直徑)은 유전력(遺傳力)이 높고 기타형질(其他形質)의 유전력(遺傳力)은 제(第)4표(表)에서와 같이 보다 낮으며 환경상관(環境相關)은 표현형상관(表現型相關), 유전상관(遺傳相關)보다 전체적(全體的)으로 낮다. 그리고 죽간재적(竹稈材積)에 대(對)하여 근원직경(根元直徑)과 흉고직경(胸高直徑)은 높은 상관관계(相關關係)를 나타내었다. 형질별(形質別) 상관관계(相關關係), 경로계수(經路係數) 및 죽간재적(竹稈材積)에 미치는 직접(直接), 간접효과(間接効果)는 도표(圖表) 1과 제(第)5표(表)에서 볼 수 있으며 결과적(結果的)으로 본시험(本試驗)에서는 죽간재적수량(竹稈材積收量)은 근원직경(根元直徑)과 흉고직경(胸高直徑)에 의(依)하여 좌우(左右)될 수 있다는 것을 알았다.