• Journal of agriculture & life science
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• v.46 no.1
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• pp.113-121
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• 2012

A total of 150,624 records of Holstein milk production collected from 2005 to 2009 were analyzed to investigate the effects of two different contemporary group definitions, parity and somatic cell score (SCS). The first definition (H BY S) of contemporary group was milking cows and heifers born in the same year and season. And the second thing (H CY S) was milking cow and heifers that delivered calves in the same year and season. Effects of contemporary group, parity and regression effect on SCS from two models were highly significant sources of variation. Coverage of variation ($R^2$) was somewhat higher in models with H BY S as contemporary group. From multivariate models with H BY S, phenotypic correlation coefficients of milk components were estimated high and positive. However, the phenotypic correlation coefficient between milk yield and SCS was -0.09, which was low enough to evidence no correlation between them. Phenotypic correlation between SCS and butter fat or between SCS and protein were also negligible but negative. From multivariate models with H CY S as contemporary group, phenotypic correlation among milk traits and SCS were similar to the estimates from models with H BY S. However, SCS in these models were lowly but negatively correlated with milk yield, milk protein, butter fat or SNF, and the phenotypic correlation coefficients of which were -0.10, -0.08, -0.08, -0.11, respectively.

• BMB Reports
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• v.55 no.11
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• pp.565-570
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• 2022

Pulmonary arterial hypertension (PAH) is a progressive and devastating disease whose pathogenesis is associated with a phenotypic switch of pulmonary arterial vascular smooth muscle cells (PASMCs). Bone morphogenetic protein (BMP) signaling and potassium two pore domain channel subfamily K member 3 (KCNK3) play crucial roles in PAH pathogenesis. However, the relationship between BMP signaling and KCNK3 expression in the PASMC phenotypic switching process has not been studied. In this study, we explored the effect of BMPs on KCNK3 expression and the role of KCNK3 in the BMP-mediated PASMC phenotypic switch. Expression levels of BMP receptor 2 (BMPR2) and KCNK3 were downregulated in PASMCs of rats with PAH compared to those in normal controls, implying a possible association between BMP/BMPR2 signaling and KCNK3 expression in the pulmonary vasculature. Treatment with BMP2, BMP4, and BMP7 significantly increased KCNK3 expression in primary human PASMCs (HPASMCs). BMPR2 knockdown and treatment with Smad1/5 signaling inhibitor substantially abrogated the BMP-induced increase in KCNK3 expression, suggesting that KCNK3 expression in HPASMCs is regulated by the canonical BMP-BMPR2-Smad1/5 signaling pathway. Furthermore, KCNK3 knockdown and treatment with a KCNK3 channel blocker completely blocked BMP-mediated anti-proliferation and expression of contractile marker genes in HPAMSCs, suggesting that the expression and functional activity of KCNK3 are required for BMP-mediated acquisition of the quiescent PASMC phenotype. Overall, our findings show a crosstalk between BMP signaling and KCNK3 in regulating the PASMC phenotype, wherein BMPs upregulate KCNK3 expression and KCNK3 then mediates BMP-induced phenotypic switching of PASMCs. Our results indicate that the dysfunction and/or downregulation of BMPR2 and KCNK3 observed in PAH work together to induce aberrant changes in the PASMC phenotype, providing insights into the complex molecular pathogenesis of PAH.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2003.06a
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• pp.105-105
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• 2003

• Journal of Plant Biotechnology
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• v.2 no.1
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• pp.25-28
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• 2000

The anthocyanin gene encoding flavonoid 3',5'-hydroxylase(F3,5H) was normally expressed in Nicotiana tobacco (Xanthi) plants cocultivated with Agrobacterium tumefaciens LBA4404 carrying egg plant flavonoid 3',5'-hydroxylase cDNA. Northern blot analysis showed the normal expression of F3', 5'H gene from transgenic plants. Here we found the phenotypic differences between transgenic plants and wild-type plants. The petal shape of transgenic plants showed more round shape and around petal tube area was compared to that of wild-type tobacco plants. And the petal color of transgenic plants was much lighter than that of wild-type tobacco plants.

• Journal of Microbiology and Biotechnology
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• v.17 no.6
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• pp.985-992
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• 2007

The diversity of archaeal strains from six hypersaline environments in Turkey was analyzed by comparing their phenotypic characteristics and 16S rDNA sequences. Thirty-three isolates were characterized in terms of their phenotypic properties including morphological and biochemical characteristics, susceptibility to different antibiotics, and total lipid and plasmid contents, and finally compared by 16S rDNA gene sequences. The results showed that all isolates belong to the family Halobacteriaceae. Phylogenetic analyses using approximately 1,388 bp comparisions of 16S rDNA sequences demonstrated that all isolates clustered closely to species belonging to 9 genera, namely Halorubrum (8 isolates), Natrinema (5 isolates), Haloarcula (4 isolates), Natronococcus (4 isolates), Natrialba (4 isolates), Haloferax (3 isolates), Haloterrigena (3 isolates), Halalkalicoccus (1 isolate), and Halomicrobium (1 isolate). The results revealed a high diversity among the isolated halophilic strains and indicated that some of these strains constitute new taxa of extremely halophilic archaea.

• ALGAE
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• v.21 no.4
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• pp.349-375
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• 2006

The application of modern ecological, ultrastructural and molecular methods, aided by the cultivation of numerous cyanobacterial morphotypes, has substantially changed our knowledge of these organisms. It has led to major advances in cyanobacterial taxonomy and criteria for their phylogenetic classification. Molecular data provide basic criteria for cyanobacterial taxonomy; however, a correct phylogenetic system cannot be constructed without combining genetic data with knowledge from the previous 150 years research of cyanobacterial diversity. Thus, studies of morphological variation in nature, and modern morphological, ultrastructural, ecophysiological and biochemical characters need to be combined in a “polyphasic” approach. Taxonomic concepts for generic and infrageneric ranks are re-evaluated in light of combined phenotypic and molecular criteria. Despite their usefulness in experimental studies, the limitations of using strains from culture collections for systematic and nomenclatural purposes is highlighted. The need for a continual revision of strain identification and proper nomenclatural practice associated with either the bacteriological or botanical codes is emphasized. Recent advances in taxonomy are highlighted in the context of prospects for understanding cyanobacterial diversity from natural habitats, and the evolutionary and adaptational processes that cyanobacteria undergo.

• Journal of Korea Multimedia Society
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• v.17 no.12
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• pp.1521-1527
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• 2014

This paper presents to design User eXperience(UX) of anthropomorphic animal characters search system (hereinafter, AACSS) for efficient user search. To this end, meta data were utilized herein to elevate the search efficiency of multimedia information and text information. Anthropomorphic animal characters require the human elements and the animal elements, thus this paper extracted the key elements of meta data as below; phenotypic element in animal system classification (Morphologic property elements, Ecological property elements, Behavioral property elements), emotion classification, which is the trait of personification and the Step of Anthropomorphic Animal Characters.

• Mycobiology
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• v.36 no.4
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• pp.203-210
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• 2008

In previous studies investigating the genus Xanthoparmelia, thirteen different species have been reported from South Korea alone. However, there currently has been no revisional study performed until now. To explore the genus Xanthoparmelia, a phenotypic analysis was performed based on morphological, anatomical and chemical characters, while an investigation of Xanthoparmelia phylogeny was based on nuclear ribosomal (nr) DNA ITS sequences. A thorough examination of the specimens deposited in the Korean Lichen Research Institute (KoLRI) confirmed that eight species of Xanthoparmelia occur inside South Korea. Our analysis further confirmed the colors of the lower surface and medullar chemistry are important taxonomic characters in Xanthoparmelia. This study also presents a detailed description of each species and a key to the genus.

• Journal of Sericultural and Entomological Science
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• v.42 no.2
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• pp.86-92
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• 2000

Studies were carried out to investigate the phenotypic characteristics of the non-molting mutant (nm-f) which was mapped on the 2nd linkage group. The results obtained were as follows : The nm-f mutant was distinguishable in the 3rd day of hatching. About 80 percentage of the non-molting mutant larvae died at the first instar within 10 days of hatching. The remaining larvae survived to the 2nd ad the 3rd instar but did not live to the final instar. There was no difference in non-molting nutant manifestation between hibernating and artificial hatching eggs. As a result of hemolymph protein analysis, the protein content on nm-f mutant was less than the normal larvae's. Therefore, we conclude that the characterization of nm-f is similar to the already known strains of non-molting mutant and the shortage of hemolymph protein is closely related to the non-molting characteristic in nm-f.

• Journal of Microbiology
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• v.41 no.2
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• pp.83-88
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• 2003

The taxonomic position of actinomycete strain SCNU5$\^$T/, isolated from extensive foam in the aeration basin of an activated sludge process, was clarified by phenotypic, chemotaxonomic and phylogenetic analyses. The strain possesses wall chemotype IV, MK-9(H$\^$0/), as the major menaquinone, and contains saturated, monounsaturated and 10-methyl branched fatty acids. The G+C content of its DNA is 68.1 mol%. Phenotypic data and DNA relatedness to known species indicate that the strain SCNU5$\^$T/ represents a new species within the genus Tsukamurella, for which we propose the name Tsukamurella sunchonensis SP. NOV. The type Strain Of T. sunchonensis is SCNU5$\^$T/ (=KCTC 9827$\^$T/).