• Natural Product Sciences
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• 제4권3호
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• pp.153-157
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• 1998

The aqueous ethanolic leaf extract of Ailanthus altissima was found to contain the new natural product, $luteolin\;7-O-{\beta}-(6"-galloylglucopyranoside)$, 13, along with fourteen known phenolic metabolites (1-12, 14 and 15). Structures of all compounds (1-15) were established by conventional methods of analysis and confirmed by FAB-MS, $^1H-\;and\;^{13}C-NMR$ spectral analysis.

• 산업식품공학
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• 제15권3호
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• pp.195-202
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• 2011

Physiological characteristics of brown rice and black rice were investigated to provide the fundamental information of physiological property of rice and to show the potential of rice as a functional ingredient. Bioactive compounds were extracted from brown and black rices with aqueous solvents like 80% ethanol. Total phenolics, flavonoids and antioxidative capacity of brown and black rices' extracts were determined. Brown rice showed much higher amount of total phenolics and flavonoid contents as well as antioxidative capacity than those of milled rice indicating that most of bioactive compounds are located in the bran layer. Black rice showed higher total phenolics and flavonoid contents and antioxidative capacity than those of brown rices. The highest antioxidative capacity was obtained from Heugjinju followed by Heugseol, Sinnongheug-chal, Hopum and Samkwang. This result indicated that antioxidative capacity is affected by total phenolics and flavonoid contents. Both brown and black rices contained higher amount of ferulic acid than that of p-coumaric acid.

• Archives of Pharmacal Research
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• 제5권2호
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• pp.71-77
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• 1982

Improvements in the analytical methodology used in the gas chromatographic/mass spectral analysis of phenolic compounds from cigarette smoke are described. For the direct analysis of crude samples, pyridine extraction and the glass capillary column GC was used for the separation of phenolics as trimethylsilyl derivativatives. The separations of cigarette smoke on Carbowax 20M and SE-54 wall coated open tubular columns are given. Improved methodology for the routine quantitation of the identified components using the computer-controlled multiple ion selection technique of MS presented. Considerations pertaining to routine analyses of a multitude of complex smoke samples are also discussed.

• Bulletin of the Korean Chemical Society
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• 제35권6호
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• pp.1763-1768
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• 2014

In the course of our program to search for antithrombotic and anti-inflammatory agents from plants, twelve phenolics (1-12) were isolated from the stems of Parthenocissus tricuspidata. Their structures were elucidated on the basis of spectroscopic (1D and 2D NMR, and MS) data analyses, and comparison with published data. At the concentration of $100{\mu}g/ml$, compounds 2, 4, 6 and 10 possessed potential effects on anti-blood coagulation, with inhibitory percentage of 216, 174, 148 and 225%, respectively; while aspirin used as positive control showed 181% inhibition at the same concentration. Furthermore, the anti-inflammatory activity of isolated compounds (1-12) was investigated on lipopolysaccharide (LPS)-induced murine macrophage cells (RAW264.7). Compounds 2, 4 and 6 also potential inhibited the production of nitric oxide, with $IC_{50}$ values of $11.9{\pm}0.3$, $2.9{\pm}0.2$ and $29.0{\pm}0.6{\mu}M$, respectively. Celastrol, the positive control used, gave an $IC_{50}$ value of $1.0{\pm}0.1{\mu}M$.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.297-303
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• 2020

Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities were investigated for comparison of the biological activity of the berry extracts. Chlorogenic acid was the major phenolic acid, which had the highest content in aronia, followed by blueberry. The rutin and isoquercetin contents were the highest in mulberry, followed by aronia, black raspberry, and blueberry. The anthocyanin content was the highest in black raspberry. Aronia showed the highest DPPH free radical and nitrite scavenging activities with the highest contents of total phenolics and proanthocyanidins.

• 한국식물병리학회지
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• 제3권1호
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• pp.3-7
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• 1987

도열병에 대해 감수성을 보이는 낙동과 성체식물저항성을 보이는 도봉의 건전엽과 도열병 감염엽내의 에탄올가용성 아미노산과 페놀화합물을 양적으로 분석하였다. 접종 3일후에는 가용성 아미노산과 페놀화합물의 수준은 건전엽과 비슷하였다. 도열병 병반이 접종 5일 후에 감염엽에 나타났을 때 가용성 아미노산과 페놀화합물은 5엽기와 3엽기에서 증가하기 시작하였고, 접종 7일 후에서는 이들 화합물의 수준은 5엽기와 8엽기에서 건전엽보다 약 $1.5\~3$배 더 많았다. 도열병 감염동안 비록 도봉이 도열병에 대해 낙동보다 덜 감염되었지만 5엽기와 8엽기의 건전엽과 감염엽에서 성체식물저항성 품종 도봉의 가용성 아미노산 및 페놀화합물 함량은 감수성 품종 낙농의 함량보다 높았다. 벼품종 도봉이 도열병에 감염동안 아미노산과 페놀화합물 함량이 현저하게 증가하여 성체식물저항성이 발현하는데 중요한 역할을 할지 모른다.

• 한국식품영양과학회지
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• 제45권3호
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• pp.333-341
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• 2016

본 연구는 물과 ethanol을 추출용매로 사용한 번행초(Tetragonia tetragonioides) 추출물이 미용 식품 소재로 사용 가능한 기능성을 증명하고자 하였다. Phenolic compounds는 물 추출물에서 3.29 mg/g, 50% ethanol 추출물에서 4.14 mg/g의 결과를 각각 나타내었다. 전자공여능 측정 결과 $200{\mu}g/mL$ phenolic compounds의 농도에서 물 추출물은 98.45%, 50% ethanol 추출물은 91.20%의 활성을 나타내었다. ABTS radical 활성 측정 결과 $100{\mu}g/mL$ phenolic compounds의 농도에서 물 추출물은 97.28%, 50% ethanol 추출물은 97.03%의 활성을 나타내었다. Antioxidant protection factor(PF) 측정 결과 $200{\mu}g/mL$ phenolic compounds의 농도에서 물과 ethanol 추출물 모두 1.77 PF의 같은 결과를 나타내었다. Thiobarbituric acid reactive substance(TBARS) 측정 결과 $100{\mu}g/mL$ phenolic compounds의 농도에서 물과 ethanol 추출물은 94.77%와 95.64%의 높은 활성을 나타내었다. 미백과 관련 있는 tyrosinase 저해 활성을 측정한 결과 $200{\mu}g/mL$ phenolic compounds의 농도에서 ethanol 추출물은 34.96%의 억제력을 나타내었다. 주름 개선 효과를 측정하는 elastase 저해 활성과 collagenase 저해 활성을 측정한 결과 $200{\mu}g/mL$ phenolic compounds의 농도에서 ethanol 추출물만 78.90%와 61.3%의 높은 저해 활성을 나타내었다. 수렴 효과를 나타내는 astringent effect에서 $200{\mu}g/mL$ phenolic compounds 농도의 ethanol 추출물은 7.82%의 효과를 나타내었다. Hyaluronidase 저해 효과 측정 결과 물 추출물 $200{\mu}g/mL$ phenolic compounds의 농도에서 81.04%의 염증 억제 효과를 나타내었다. 이러한 결과들로 미루어 보았을 때 번행초 추출물은 항산화 작용과 미용 식품으로서의 기능성을 기대할 수 있다.

• 한국작물학회지
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• 제56권4호
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• pp.375-384
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• 2011

The aim of this study was to evaluate 30 phenolic compounds in adzuki bean germplasm. Adzuki 21653 had the highest content of total phenolics compounds ($6597\;{\mu}g\;g^{-1}$) while 104372 had the lowest concentration. The average total phenolic content of Japanese ($2432\;{\mu}g\;g^{-1}$) adzuki beans was higher than that of Korean ($2256\;{\mu}g\;g^{-1}$) adzuki beans. The average total phenolic contents were $2507\;{\mu}g\;g^{-1}$ in small sized adzuki beans from Japan and $2459\;{\mu}g\;g^{-1}$ in those from Korea. In large sized adzuki beans, the average total phenolic contents were $1315\;{\mu}g\;g^{-1}$ in Japanese seeds and $1232\;{\mu}g\;g^{-1}$ in Korean seeds. The average total phenolic contents in medium seeds were $2369\;{\mu}g\;g^{-1}$ in Japanese adzuki beans and $1397\;{\mu}g\;g^{-1}$ in Korean ones. In small seeds, the total phenolic contents of adzuki beans varied from $524\;{\mu}g\;g^{-1}$ to $6597\;{\mu}g\;g^{-1}$ in Japanese ones and from $375\;{\mu}g\;g^{-1}$ to $6569\;{\mu}g\;g^{-1}$ in Korean ones. Japanese and Korean adzuki beans were divided into landraces and wild adzuki beans. In this study, the wild adzuki beans showed higher contents of total phenolics than the native varieties. Specifically, the wild adzuki beans from Korea had the highest concentration of phenolics ($3403\;{\mu}g\;g^{-1}$). All adzuki bean germplasms were measured for their color and were classified into four groups accordingly: A; L < 30, +a, +b; B; L < 30, +a, -b, C; L > 50, +a, +b, D; L > 50, +a, -b. Especially, group B had the highest concentration of total phenolic compounds ($2827\;{\mu}g\;g^{-1}$), whereas group C had the lowest concentration ($1882\;{\mu}g\;g^{-1}$).

• 한국식품과학회지
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• 제49권6호
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• pp.714-716
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• 2017

장미꽃을 $30^{\circ}C$, 상대습도 60%의 조건에서 24시간 동안 숙성하여 총페놀화합물 함량, 산화방지 활성, 카테킨 함량을 최대로 증가시키는 조건을 수립하였다. 장미꽃을 차로 이용하기 전에 숙성공정을 통하여 생리활성 물질인 카테킨을 비롯한 페놀화합물의 추출 효율을 증가시킬 수 있고 이에 따라 장미꽃 차의 산화방지효과를 증대 시킬 수 있다.

• Journal of Applied Biological Chemistry
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• 제55권4호
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• pp.207-215
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• 2012

유근피에 함유된 페놀성 물질은 70% ethanol을 용매로 하여 추출하였을 때 $17.9{\pm}1.0mg/g$이 용출되었다. 주름 생성과 관련된 elastase 저해 활성은 $50-200{\mu}g/mL$ phenolics 농도의 70% ethanol 추출물에서 55.5-69.5%의 제해효과를 확인할 수 있었다. 유근피 추출물의 미백효과와 관련된 tyrosinase 저해활성은 $200{\mu}g/mL$ phenolics 농도의 70% ethanol 추출물에서 24%의 저해효과가 측정되었다. Astringent 활성은 $200{\mu}g/mL$ phenolics 농도의 70% ethanol 추출물에서 71%의 활성을 나타내어 모공축소 효과가 높을 것으로 판단되었다. 유근피 추출물의 hyaluronidase 저해활성은 $50{\mu}g/mL$ phenolics의 농도에서 80%의 염증억제 효과가 확인되었다. 유근피 추출물의 항균활성은 물 추출물의 경우 $50-200{\mu}g/mL$의 phenolics 농도에서 Propionibacterium acnes에 대한 항균활성이 각각 8.7, 10, 11.1 및 11.8 mm의 clear zone이 확인되었다. 유근피 추출물을 첨가하여 제조한 복합 기능성 화장품(로션)의 안정성은 28일 동안 pH 및 점도의 변화가 없었고, 온도 및 광에 대하여 안정하였다. 제조한 로션은 추출물의 첨가 농도가 증가함에 따라 색도는 어둡게 나타났으나, 사용감면에서 8.5점 정도의 좋은 점수를 나타내었다.