• Title/Summary/Keyword: phenolic components

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The Inhibitory Activity of Erigeron annuus-Derived Components on $Interferon-{\gamma}$ and Lipopolysaccharide-Induced Nitric Oxide Production in Mouse Pheritoneal Macrophage

  • Lee, Hee-Jung;Kim, You-Ah;Jeong, Na-Ho;Hong, Seung-Heon;Seo, Young-Wan
    • Journal of Applied Biological Chemistry
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    • v.50 no.3
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    • pp.160-163
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    • 2007
  • Two flavonoids (1 and 2) and one phenolic acid (3) obtained from Erigeron annuus have recently been shown to have potent antioxidant activities. Aim of this study was to investigate the inhibitory effects of these components on $interferon-{\gamma}$ and lipopolysaccharide-induced nitric oxide productions in the mouse pheritoneal macrophage. Compounds 2 and 3 showed marked inhibitory activities against inducible nitric oxide synthase (iNOS) on the lipopolysaccharide and $interferon-{\gamma}-stimulated$ mouse pheritoneal macrophages without cytotoxicity. Therefore, these results suggest that the compounds could be effective anti-inflammatory agents as nitric oxide inhibitors in vivo.

GC-MS Analysis of the Extracts from Korean Cabbage (Brassica campestris L. ssp. pekinensis ) and Its Seed

  • Hong, Eunyoung;Kim, Gun-Hee
    • Preventive Nutrition and Food Science
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    • v.18 no.3
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    • pp.218-221
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    • 2013
  • Korean cabbage, a member of the Brassicaceae family which also includes cauliflower, mustard, radish, and turnip plants, is a crucial leafy vegetable crop. Korean cabbage is harvested after completion of the leaf heading process and is often prepared for use in "baechu kimchi", a traditional Korean food. Many of the components in Korean cabbage are essential for proper human nutrition; these components can be divided into two groups: primary metabolites, which include carbohydrates, amino acids, fatty acids, and organic acids, and secondary metabolites such as flavonoids, carotenoids, sterols, phenolic acids, alkaloids, and glucosinolates (GSLs). Using gas chromatography-mass spectrometry, this study examined the variety of volatile compounds (including isothiocyanates) contained in Korean cabbage and its seed, which resulted in the identification of 16 and 12 volatile compounds, respectively. The primary volatile compound found in the cabbage was ethyl linoleolate (~23%), while 4,5-epithiovaleronitrile (~46%) was the primary volatile component in the seed.

Antioxidant effects and tyrosinase and elastase inhibitory activities of mountain ginseng adventitious roots extracts at different ethanol concentrations

  • Ko, Hyun Min;Eom, Tae Kil;Kim, Kyeoung Cheol;Kim, Chul Joong;Lee, Jae Geun;Kim, Ju-Sung
    • Korean Journal of Agricultural Science
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    • v.45 no.3
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    • pp.499-508
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    • 2018
  • To use mountain ginseng adventitious roots (MGAR), which is superior in functionality compared to ginseng, and to mass produce raw materials as a functional cosmetic material, the antioxidant and cosmeceutical activities of the extracts of MGAR at different ethanol concentrations (0, 20, 40, 60 and 80%) were compared and analyzed. At first, different ethanol concentrations were compared to determine the best solvent for the extraction of phenolic compounds from MGAR. An 80% ethanol extract with 10.07 mg of gallic acid equivalents/g sample and 0.94 mg of quercetin equivalents/g sample was the best solvent in the extraction of phenolic compounds. However, the 60% ethanol extract had the highest antioxidant activity, evident by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, Trolox equivalent antioxidant capacity (35.01 mM Trolox/g sample) and ferric reducing antioxidant power (460.94 mM $FeSO_4/g$ sample). However, the inhibitory activities of the 80% ethanol extract from the MGAR against tyrosinase and elastase were higher than those of the other extracts. The results of this study show that the extract of MGAR can be used as an easily accessible source of natural antioxidant and as a possible cosmetic ingredient in the cosmeceutical industry. However, the components responsible for the antioxidant and cosmeceutical activities of the MGAR extracts are currently unclear. Therefore, it is suggested that further studies should be carried out to isolate and identify the antioxidant and cosmeceutical components in MGAR.

A comparison of the characteristic properties between soybean (Glycine max [L.] Merrill) seeds with different seed coat colors

  • Oh, Sung-Dug;Yeo, Yunsoo;Lee, So-Young;Suh, Sang Jae;Moon, Jung Kyung;Park, Soo-Kwon;Park, Soo-Yun
    • Korean Journal of Agricultural Science
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    • v.46 no.4
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    • pp.971-980
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    • 2019
  • We profiled the health-promoting bioactive components in nine types of soybean seeds with different seed coat colors (yellow, green, brown, and black) and investigated the effects of different extraction solvents (methanol, ethanol, and water) on their antioxidant activities. The carotenoid and anthocyanin compositions varied greatly by seed color, and the phenolic acids, total phenol, and total flavonoid contents differed by genotype. The carotenoid content was relatively higher in soybean seeds with green and black seed coats than in those with a yellow seed coat while lutein was the most plentiful. The anthocyanin content was considerably higher in the soybean seed with the black seed coat. The results of the DPPH assay showed strong antioxidative activities in the methanol- and water-extracts compared to the ethanol-extract, irrespective of the seed coat colors. Moreover, the soybean seeds with the black seed coat exhibited the highest antioxidant activity among the samples, regardless of the extraction solvent used. Eighteen bioactive compounds were subjected to data-mining processes including principal component analysis and hierarchical clustering analysis. Multivariate analyses showed that brown and black seeds were distinct from the yellow and green seeds in terms of the levels of carotenoids and anthocyanins, respectively. These results help our understanding of the compositional differences in the bioactive components among soybean seeds of various colors, providing valuable information for future breeding programs that seek to enhance the levels of compounds with health benefits.

Changes in Color Value and Chemical Components of Hoelen by Various Drying Methods (건조방법에 따른 복령의 색도 및 화학성분의 변화)

  • Jee, Jae-Hyung;Lee, Hyun-Dong;Chung, Shin-Kyo;Choi, Jong-Uck
    • Korean Journal of Food Science and Technology
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    • v.31 no.3
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    • pp.575-580
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    • 1999
  • In this study, the quality changes of Hoelen was investigated, which were color differences, browning degrees, total phenolic compounds and electron donating abilities to optimize drying condition using hot air and far infrared drying by changing conditions such as temperatures, air velocities and radiation distances. In color values, ${\Delta}b\;value$ decreased with increasing temperatures, while ${\Delta}L$, ${\Delta}a$ and ${\Delta}E\;value$ increased. The browning degrees showed the similar tendency with the color values. Total phenolic components of Hoelen decreased with increasing drying temperature and air velocity, and decreasing radiation distance. On the other hand, there was no significant difference in the changes of electron donating abilities (EDA) of Hoelen among drying methods and conditions. As a result, the hot air drying was more effective method than the far infrared was.

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Analysis of Total Phenolic, Flavonoid Contents, and Antioxidant Capacity Extract from Leaves of Selected Accessions of Two Wild Pear Species, Pyrus pyrifolia and P. ussuriensis

  • Yang, Si Woong;Lee, Hwa;Song, Jae Mo;Choi, Sun Eun;Cheong, Eun Ju
    • Journal of Forest and Environmental Science
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    • v.37 no.3
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    • pp.226-234
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    • 2021
  • Two species, P. pyrifolia and P. ussuriensis, of the genus Pyrus native to Korea, are valuable genetic resources that can be used for food, dietary supplement, pharmaceutical, and cosmetics. Bioactive compounds of the plant leaves are the main components that are used for the products. Farmers had cultivated a few individuals from the wild to produce fruits or leaves for traditional remedy or tea; however, bioactive components of their leaves are not tested. We selected some trees from the natural stand that have distinct traits for the improvement program. We investigated the bioassay on the extracts' bioactive compounds and antioxidant capacity from the selected accessions and other accessions, including newly developed cultivars. The contents of the phenolic compounds and flavonoids from the leaf extracts of the selected accessions were higher than the commonly cultivated trees in both species but lower than 'Sanhyang' in P. ussuriensis. The antioxidant capacity was measured using two assay methods, DPPH and ABST. The selected cultivars also had higher inhibitory activity than common trees. The selected accession 'Cultivar 3' in P. pyrifolia had the highest radical scavenging activity than others. Although leaves of the accessions were used only in this study, all three selected individuals have the potential for cultivar in containing high bioactive compounds and antioxidant capacity.

STUDY OF VOLATILE COMPONENTS IN THE PYROLYZATES OF COCOA POWDER TOBACCO PRODUCTS FLAVORANT (담배향료로 쓰이는 코코아분말의 열분해 생성물에 관한 연구)

  • Park, Joon Y.;Kim, Ok C.;Na, Do Y.;Chang, Hee J.;Kim, Yong T.
    • Journal of the Korean Society of Tobacco Science
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    • v.12 no.2
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    • pp.67-75
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    • 1990
  • The pyrolytlc behavior of cocoa powder, a flavorant of tobacco smoking products, was examined by determining its pyrolyzate constituents. Cocoa powder was pyrolyzed of two cigarette smoking conditions'distillation-pyrolysis zone(35$0^{\circ}C$, 55$0^{\circ}C$) and high temperature zone($650^{\circ}C$, 85$0^{\circ}C$). Pyrolyzate was flushed from the tube by NB gas into CS2 trap in dry ice-acetone cooled bath and charcoal tube and its constituents were analyzed by GC/MS. As results, the major components of pyrolyzates were identified as hydrocarbon and phenolic compounds. In addition to these, aldehyde, ketone, pyrazin in very small amount. Component changes were observed with temperature increase; decane, styrene, tridecane, m-cresol,4-ethylphenol were increased while hexadecane, tetradecane were decreased. o-cresol and 2-ethylphenol were constant in amounts despite temperature change.

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Physiological and pharmacological features of the non-saponin components in Korean Red Ginseng

  • Hyun, Sun Hee;Kim, Sung Won;Seo, Hwi Won;Youn, Soo Hyun;Kyung, Jong Soo;Lee, Yong Yook;In, Gyo;Park, Chae-Kyu;Han, Chang-Kyun
    • Journal of Ginseng Research
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    • v.44 no.4
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    • pp.527-537
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    • 2020
  • Panax ginseng, a medicinal plant, has been used as a blood-nourishing tonic for thousands of years in Asia, including Korea and China. P. ginseng exhibits adaptogen activity that maintains homeostasis by restoring general biological functions and non-specifically enhancing the body's resistance to external stress. Several P. ginseng effects have been reported. Korean Red Ginseng, in particular, has been reported in both basic and clinical studies to possess diverse effects such as enhanced immunity, fatigue relief, memory, blood circulation, and anti-oxidation. Moreover, it also protects against menopausal symptoms, cancer, cardiac diseases, and neurological disorders. The active components found in most Korean Red Ginseng varieties are known to include ginsenosides, polysaccharides, peptides, alkaloids, polyacetylene, and phenolic compounds. In this review, the identity and bioactivity of the non-saponin components of Korean Red Ginseng discovered to date are evaluated and the components are classified into polysaccharide and nitrogen compounds (protein, peptide, amino acid, nucleic acid, and alkaloid), as well as fat-soluble components such as polyacetylene, phenols, essential oils, and phytosterols. The distinct bioactivity of Korean Red Ginseng was found to originate from both saponin and non-saponin components rather than from only one or two specific components. Therefore, it is important to consider saponin and non-saponin elements together.

Antioxidative Activity and Related Compounds of Apple Pomace (사과박의 항산화 활성 및 항산화 성분)

  • Lee, Jae-Ho;Kim, Young-Chan;Kim, Mi-Yeon;Chung, Hun-Sik;Chung, Shin-Kyo
    • Korean Journal of Food Science and Technology
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    • v.32 no.4
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    • pp.908-913
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    • 2000
  • To enhance the utilization of apple pomace for the functional food resources, we analyzed the useful components and examined the antioxidant activity of apple pomace. The contents of total dietary fiber, total flavonoid, total phenolic acid and vitamine C were 55.56%, 458 mg%, 1048 mg% and 19.8 mg%, respectively. Protocatechuic acid, cinamic acid, caffeic acid, ferulic acid, syringic acid, vanillic acid and phydroxybenzoic acid were identified in the apple pomace extract by GC-MS. Phloridzin and quercetin-3-glucoside were identified in the apple pomace extract by HPLC. Ethyl acetate fraction showed the highest antioxidative ability by ferric thiocyanate method. Malondialdehyde(MDA) formation in normal rat liver tissue also showed the lowest in ethyl acetate fraction.

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Potential of Wood Vinegar in Enhancing Fruit Yield and Antioxidant Capacity in Tomato

  • Benzon, Hiyasmin Rose L.;Lee, Sang Chul
    • Korean Journal of Plant Resources
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    • v.29 no.6
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    • pp.704-711
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    • 2016
  • Tomatoes are considered as one of the main components of daily meals in most households. Thus, it is important to invest in studies enhancing their yield and nutritional value. The study evaluated the effect of wood vinegar (WV) on tomato under greenhouse conditions. Data on fruit number, fruit weight, and plant height were recorded. Total phenolic content (TPC) and antioxidant activity of tomato were determined using the Folin-Ciocalteu reagent and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, respectively. FRR-CF+500x-WV significantly increased the fruit number (86.11%) and fruit weight (81.78%) over the control. Results were comparable to HRR-CF+250x-WV, indicating that conventional fertilizer and WV may have synergistic effect on each other. TPC and scavenging effect on DPPH radical was improved by 250x-WV application. The increase in the phenolic compounds can be attributed to WV as a direct source or as a factor triggering the plants to produce more secondary metabolites with the purpose of increasing natural defenses. The significant effect obtained by applying the full recommended rate of conventional fertilizer on the carotenoid content was due to the availability of the major nutrients needed by the plant. Correlation analysis showed that carotenoids have negative correlation with TPC and DPPH. However, TPC and DPPH showed that these are positively correlated with each other. Tomatoes are excellent source of antioxidants associated with the reduction of some human diseases and improved health. The results provided evidence that WV alone and/or its combination with conventional fertilizers has favorable effects on the quality of tomato.