• Fisheries and Aquatic Sciences
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• 제3권3_4호
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• pp.195-199
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• 2000

Tyrosinase inhibitory activities of 14 kinds of seaweed, Ecklonia stolonifera, Ecklonia cava, Undaria pinnatiflda, Laminaria japonica, Sargassum fulvellum, Sargassum miyabei, Sargassum thunbergii, Porphyra yezoensis, Gracilaria verrucosa, Carpopeltis affinis, Pachymeniopsis elliptica, Gelidium amansii, Codium fragile and Ulva pertusa were determined using commercially available mushroom tyrosinase in an in vitro assay system. The $1\%$ (w/v) methanol extract from E. stolonifera showed the highest tyrosinase inhibitory activity of $79.0\%$, electron donating activity of $79.0\%$ and total phenol content of 3.75 mg/100g. Ethyl acetate-methanol-water (7 : 2 : 0.2, v/v) fraction $(0.5\%,\;w/v)$ isolated from the methanol extract showed tyrosinase inhibitory activity of $75.9 \%$, electron donating activity of $88.1 \%$ and total phenol content of 4.38 mg/100g. Tyrosinase inhibitory activity was closely associated with total phenol content (R = 0.99) and electron donating activity (R=0.99). Maximum absorption wavelength of the fraction was 218nm and that of phenolic compounds showed about a range from 210 to 220nm. The inhibition mode of the fraction was noncompetitive inhibition.

• 한국식품저장유통학회지
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• 제15권3호
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• pp.325-331
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• 2008

The propose of this study was to investigate the antioxidant activity of 80% ethanol extracts and various solvent fractions of dandelion (Taraxacmn officinale) leaves and roots, Total phenolics and phenolic acid contents were also examined. The total phenol content of leaves and roots were $7.9{\pm}0.4%$ and $9.4{\pm}0.3%$ respectively. Eight phenolic acids were separated by GC, among which caffeic acid (113.7 mg%)and m-coumaric acid (152.6 mg) were the dominant phenolic acids in leaves and roots, respectively. Amongst solvent functions of leaves and roots, the ethyl acetate fraction showed the strongest radical scavenging activity. A strong correlation was found between total phenol content and electron-donating ability, and ABTS radical scavenging activity showed a similar trend as electron-donating ability. Hydroxyl-radical-scavenging activity and lipid peroxidation were significantly higher in the ethyl acetate fraction than other factions. In particular, the SOD-like activity was highest (43.6%) in the ethyl acetate fraction of dandelion leaves, and was higher than that of trolox. Thus, the ethyl acetate fraction of dandelion leaves exhibited significant phenol content, antioxidant activity, and free-radical-scavenging effects.

• 대한본초학회지
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• 제30권4호
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• pp.101-108
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• 2015

Objectives : Positive effects of Ginseng has great research attentions such as anticancer, anti-diabetic, antiaging, liver, immune function, CNS, etc. In this study, we investigated Hydroponic-cultured Ginseng Folium fermented byBacillus subtilisto establish fermentation conditions for enhancing functionality.Methods : Ginseng Folium were cultivated hydroponic-cultured and were extracted with methanol. We inoculateBacillus subtilisfor fermentation by adding to 0%, 3% and 5% sugar respectively and checked antioxidant activities, total phenolic content and total saponin content in 2 days intervals during 11 days. The antioxidant activities were studied by the 1,1-diphenyl-2-picryl hydrazyl(DPPH) radical, 2, 2'-Azino-bis(3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt(ABTS) radical scavenging assay and Reducing power assay. We analyzed the Total phenol content, crude saponin content and ginsenoside content. Moreever, Hepatoprotective effects by Glutamic oxaloacetic transaminase(GOT) and Glutamic pyruvic transaminase(GPT) in Sprague-Dawley rat.Results : The results of DPPH and ABTS were 66.89% and 96.72%, respectively. The reducing power was resulted in optical density of 0.7312 with 3% sugar after 9 days of fermentation. and the concentration at 200 ㎍/㎖. Total phenol content was 36.92㎎/g with 3% sugar after 9 days of fermentation, in which crude saponin content wasn't changed, and ginsenoside content such as Rg3, Re and Rb was increased. Activities of GOT and GPT concentration were decreased in rat.Conclusions : This study suggests that hydroponic-cultured Ginseng Folium fermented byBacillus subtilisin 9 days showed significant efficacy of hepato-protection as well as antioxidant compared to the others. In addition, it shows not only improved value but also utilized hydroponic-cultured Ginseng Folium by fermentation.

• 한국식품저장유통학회지
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• 제25권1호
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• pp.19-26
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• 2018

본 연구는 저장성이 낮아 폐기되는 복숭아를 활용하여 고추장을 담금하여 품질 특성을 조사하였다. pH, 당도, 염도, 환원당, 회분 및 열량은 시판 고추장에 비하여 복숭아 고추장이 낮은 경향을 보였으나, 산도 및 수분은 복숭아 과육의 높은 유기산 및 수분의 함량으로 다소 높은 결과를 얻었다. 유리당 함량의 비교에서 glucose는 시판 고추장이 복숭아 고추장에 비하여 높은 함량을 보였지만, fructose와 maltose는 복숭아 고추장에서 높은 함량을 보였다. 3대 영양소의 함량은 단백질과 탄수화물에서 시판 고추장의 함량이 높았으며, 지방은 복숭아 고추장의 함량이 높았다. 무기원소의 함량은 나트륨과 마그네슘에서 시판 고추장이 높게 측정되었으며, 칼륨 함량은 복숭아 고추장에서 높은 결과를 보였다. 페놀 및 플라보노이드의 함량을 비교한 결과, 증류수 추출물에서는 유의적인 차이가 없었지만, 페놀 함량에서 복숭아 고추장의 에탄올 추출물이 시판 고추장의 추출물 보다 더 높은 결과를 가져왔다. 또한, DPPH 및 ABTS 라디컬 소거 활성을 비교한 결과 에탄올 추출물에서는 유의적인 차이가 없었지만, 증류수 추출물에서 DPPH 라디컬 소거 활성이 더 높게 나타났다. 이와 같이 복숭아 품종별 페이스트를 이용하여 제조한 고추장의 이화학적 품질 특성을 조사한 결과, 대부분의 품종에서 시판고추장에 비해 품질이 높게 나타나 상품화의 가능성이 있는 것으로 기대된다. 향후 담금 조건, 관능적 특성, 숙성 기간 및 유통 안전성에 관한 연구가 요망되었다.

• 한국식품영양과학회지
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• 제17권2호
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• pp.85-94
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• 1988

두유의 고삽미와 불쾌취를 제거하기 위한 기초 자료를 얻기 위하여 대두에 NaOH, $NaHCO_3$ 용액을 처리하여 phenol 화합물의 변화를 HPLC로 정량하였고, 두유중의 beany flavor를 GC로 분석하였으며, 두유의 일반성분 및 기호도를 측정한 결과 대두중의 phenol 화합물은 chlorogenic, p-hydroxybenzoic, p-coumaric, ferulic, gentisic acid가 확인되었으며, chlorogenic acid 함량이 가장 높았다. 대두중의 phenol 화합물중 chlorogenic acid는 주로 결합형, 기타 다른 phenol 화합물은 유리형으로 대부분 존재하였다. 대두를 0.1% NaOH용액으로 8시간 침지하였을 때 chlorogenic acid는 85% 이상이 제거되었다. 대두중의 phenol화합물 제거에는 NaOH용액이 효과적이었고, 온도가 높아짐에 따라 제거율도 높아졌으며, 0.1% NaOH용액에게서 $90^{\circ}C$, 1시간처리하였을 때 phenol 화합물은 대부분 제거되었다. 물, 0.1% NaOH 및 0.5% $NaHCO_3$ 용액으로 침지하여 제조한 두유의 일반성분은 처리간에 비슷하였다. NaOH 용액 처리에 의하여 두유의 beany flavor 중 hexanol은 증가하였으나, hexanal, propanal, pentanal은 60% 이상이 제거되었다. NaOH 용액처리, 고온침지처리에 의하여 두유의 yellowness는 증가하였으나 물과 저온 침지처리에서는 whiteness가 증가하였다. 대두를 0.1% NaOH 용액으로 $90^{\circ}C$에서 1시간 처리하여 제조한 두유의 기호도가 가장 좋았다.

• Applied Biological Chemistry
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• 제48권1호
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• pp.65-69
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• 2005

St. John's wort를 성인병 예방을 위한 기능성 식품소재로 이용하기 위하여 추출물의 생리활성효과를 조사하였다. 추출물의 phenol 함량은 열수추출물이 $237.4{\pm}13.2\;{\mu}g/ml$였으며, 알콜추출물은 $246.0{\pm}10.5\;{\mu}g/ml$로 알콜추출물의 phenol 함량이 다소 높게 나타났다. 추출물의 항산화효과는 DPPH가 열수추출물과 알콜추출물이 각각 95.02%, 95.24%로 높게 나타났고, antioxidant protection factor는 알콜추출군이 PF 1.78정도로 지용성물질에 대한 항산화력도 높은 것으로 확인되었으나 열수추출물의 경우 지용성 물질에 대한 항산화력이 거의 없었다. Helicobacter pylori에 대한 추출물의 항균활성은 열수추출물의 경우 clear zone이 나타나지 않았으며 알콜추출물의 경우 $100\;{\mu}g/ml$과 $150\;{\mu}g/ml$의 농도에서 각각 11 mm와 13 mm의 clear zone이 관찰되어 저해활성이 높은 것으로 판단되었다. Angiotensin converting enzyme 저해효과는 열수추출군에서만 19.15%의 angiotensin converting enzyme에 대한 저해력을 나타내어 고혈압의 예방 효과는 비교적 낮았다. Xanthin oxidase에 대한 억제효과는 열수추출물이 84.79%, 알콜추출물이 100%의 완벽한 저해효과를 나타내어 xanthin oxidase에 대한 높은 저해를 관찰할 수 있었다. 이러한 결과로 St. John's wort는 Helicobacter pylori에 대한 항균제, 항산화제 및 anti-gout 소재로의 개발이 기대되었다.

• 한국식품영양학회지
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• 제26권4호
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• pp.646-654
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• 2013

This study was conducted to investigate the effect of pine needle powder on antioxidant activity and the quality characteristics of Majakgwa (Korean traditional cookie). Majakgwa was prepared with different amounts of pine needle powder (in ratios of 0, 3, 6, 9 and 12% to the flour quantity). The antioxidant activity was estimated by DPPH free radical scavenging activity and by the total phenol content in pine needle powder and Majakgwa. For analyzing the quality characteristics, bulk density and pH of the dough, moisture content, volume, color, texture profile analysis and sensory evaluations were measured. The bulk density, volume, total phenol contents and DPPH free radical scavenging activity of Majakgwa significantly increased with increasing pine needle powder (p<0.001), whereas the pH of the dough, L values and b values of the Majakgwa significantly decreased with increasing pine needle powder content (p<0.001). The consumer acceptability score for the 6% pine needle Majakgwa ranked significantly higher (p<0.001) than those of the other groups in overall preference, flavor, taste, crispiness and color. Acid value and peroxide value was lower in Majakgwa with pine needle powder than control. From these results;we suggest that pine needle powder is a good ingredient for increasing consumer acceptability and functionality of Majakgwa.

• 공업화학
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• 제8권6호
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• pp.973-978
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• 1997

Diglycidyl ether of bisphenol A(DGEBA)수지에 castor oil(CO)로 혼합한 후 경화촉매인 tris(dimethylaminomethyl) phenol (DMP-30)로 반응시킨 에폭시의 강인성과 모폴로지를 관찰하였다. 개질제인 CO와 에폭시수지의 혼합물은 기존의 에폭시 개질제인 carboxyl terminated butadiene acrylonitrile(CTBN)보다 상용성이 좋았다. 경화온도와 CO의 양이 증가할수록 유리전이온도가 감소하였는데, CO/에폭시 경화물은 경화온도가 증가할수록 가교밀도가 감소하면서 연성구조를 갖게 되는 것으로 해석되었다. CO/에폭시 경화물은 경화온도 $40^{\circ}C$에서 CO의 양이 증가함에 따라 강인성이 약간 증가하였다. 경화온도가 증가와 CO의 양이 증가함에 따라 강인성이 증가하였다.

• 한국식품조리과학회지
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• 제17권5호
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• pp.456-463
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• 2001

The proximate composition of 3 kinds of Umbelliferaeceae Wild Plants (Angelica gigas NAKAI., Ostericum sieboldii NAKAI. and Coriandrum sativum LINNAEUS) were analyzed and used for making pork sausages as a functional ingredient. In the proximate composition, crude protein content was the highest in Ostericum sieboldii, 37.87%, and crude lipid and crude ash contents were the highest in Coriandrum sativum. Vitamin C was contained in Angelica gigas at 0.30mg%. but was not detected in Ostericum sieboldii and Coriandrum sativum. $\beta$-carotene content in Angelica gigas was the highest at 19.96%, but selenium was not detected in any of them. Total phenol content in Coriandrum sativum was the highest as 1107.43mg%. TBA and VBN values of all sausages with wild plants increased gradually during four-week-storage and were lower than those of control. Coriandrum sativum-added sausage had the lowest TBA as well as VBN valuew. During the four-week-storage at 4$\^{C}$, TBA value and VBN value were in the very safe range at all sample. Total phenol contents in sausages appeared to have more antioxidative activity than vitamin C, $\beta$-carotene and selenium, and consequently decreased the TBA values of sausages.

• 한국약용작물학회지
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• 제23권3호
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• pp.207-213
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• 2015

The purpose of this study was to determine the possibility of using Wasabi japonica Matsum leaves as natural health food source. To accomplish this purpose, the contents of general and antioxidative nutrients of Wasabi japonica Matsum leaves were measured. The contents of carbohydrate, crude protein, crude lipid and ash are 53.41%, 25.00%, 7.95% and 13.64%. And the calories of Wasabi leaves was 385.23 Kcal. Total dietary fiber was 52.27%. The K was the largest mineral followed by Ca, P, Mg which means Wasabi leaves is alkali material. The contents of sinigrin and allyisothiocyanate in the wasabi leaves were 69.2 mg/g and 241.0 mg/g, respectively. Total phenol contents of the hot water extract and the 70% ethanolic extract were $19.44{\pm}0.23$ and $19.33{\pm}1.17mg$ GAE/g, respectively. The total flavonoids content of the hot water extract and the 70% ethanol extract were $7.69{\pm}0.71$ and $19.25{\pm}1.41mg$ QE/g, espectively. The general nutrients and other antioxidant bioactive materials in Wasabi japonica Matsum leaves were also potential materials for good health food.