• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.308.1-308.1
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• 2002

In drugs for neutropenia. which suppress born marrow and which are needed to control their dosage and the therapy periods, there has been lots of emphasis on drug development to increase blood cells. In order to see the effects of an impact to hematopoietic cells. the hematopoietic effect of Phellinus linteus polysaccharide by segregating the study levels in matured cells both in born marrow cell and splenocyte were examined. (omitted)

• 한국잠사곤충학회지
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• 제44권2호
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• pp.74-81
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• 2002

The optimal conditions for mycelial growth of P. linteus ASI 26011 were 25-30$^{\circ}C$ and pH 6.0, respectively. The mycelial growth of P. linteus was excellent on MCM medium. In case of carbon sources, the mycelial growth of P. linteus was best on the culture media that were contained with sucrose, mannose and glucose. Potassium nitrate and sodium nitrate were good for the mycelial growth of P. linteus as a nitrogen source. For comparison of the mycelial colonization of P. linteus on logs, several techniques of inoculation were tested; the sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich. The initial mycelial growth and the full mycelial colonization of P. linteus were the best on 20 cm logs under the condition of 42% of moisture content in log. Also the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after 5-6 months of incubation was the best for formation of basidiocarp of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at the 31-35$^{\circ}C$ and over 96% of relative humidity.

• 동의생리병리학회지
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• 제18권6호
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• pp.1825-1831
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• 2004

Phellinus linteus is known as a medicinal mushroom, which has the pharmaceutical activity on tumors and inflammatory diseases in traditional Oriental medicine. However, despite extensive pharmacological studies on P. linteus, the liquor of fermentation using mycelium of P. linteus(LFMP) has not been investigated. In the present study, it was examined the effect of the evaporating extract from LFMP(E-LFMP) on the expression of inflammatory proteins and the generation of reactive oxygen species in human hepatocarcinoma HepG2 cells. E-LFMP inhibited acetaldehyde-induced morphological change in HepG2 cells. Also, E-LFMP inhibits expression of inflammatory proteins including cyclooxygenase(COX)-1 and COX-2 through suppressing nuclear translocation of nuclear factor κB(NF-κB) and degradation of inhibitory κBα(IκBα). In addition, E-LFMP inhibits generation of reactive oxygen species(ROS) by hydrogen peroxide(H₂O₂) in HepG2 cells. These results suggest that LFMP has the pharmaceutical, especially anti-inflammatory, activity similar to P. linteus mushroom.

• Mycobiology
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• 제34권4호
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• pp.200-205
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• 2006

Phellinus genus belonged to Hymenochaetaceae of Basidiomycetes and has been well known as one of the most popular medicinal mushrooms due to high antitumor activity. This study was carried out to obtain the basic information for mycelial culture conditions of Phellinus linteus, P. baumii, and P. gilvus. According to colony diameter and mycelial density, the media for suitable mycelial growth of them were shown in MEA, glucose peptone, and MCM. The optimum temperature for mycelial growth was $30^{\circ}C$. Carbon and nitrogen sources were man nose and malt extract, respectively. The optimum C/N ratio was 10:1 to 5:1 with 2% glucose concentration, vitamin was thiamine-HCl, organic acid was succinic acid, and mineral salt was $MgSO_{4}{\cdot}7H_{2}O$.

• Korean Chemical Engineering Research
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• 제46권2호
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• pp.430-435
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• 2008

상황버섯(Phellinus linteus) 균사체로부터 얻어지는 다당류를 최대화하기 위하여 투석시간(8, 16, 24, 48 h)을 포함하는 추출조건과 정제조건이 다당류의 양에 미치는 영향에 대해 연구하였다. 추출온도(50, 65, 80, $95^{\circ}C$)와 건조 균사체의 무게에 대한 물의 부피의 비(10, 15, 20, 25 ml/g), 추출시간(2, 4, 6, 8 h), 에탄올의 최종 농도(70, 75, 80, 85%) 그리고 묵힘시간(1, 4, 8, 16 h)이 증가할수록 다당류의 양은 증가하였다. 투석시간이 24 h까지 증가할수록 다당류의 양은 감소하였으나 그 이상의 투석시간은 다당류의 양을 거의 변화시키지 못했다. 침전용매로는 3가지 용매(에탄올, 메탄올, 아세톤)를 사용하였는데, 다당류의 양은 아세톤, 에탄올, 메탄올의 순서로 감소하였다. 최적 추출조건은 추출온도가 $95^{\circ}C$이고, 건조 균사체의 무게에 대한 물의 부피의 비가 25 ml/g이며, 추출시간은 8 h인 조건이었다.

• 한국식품영양과학회지
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• 제31권4호
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• pp.636-641
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• 2002

인공재배한 상황버섯 원료의 영양성분은 탄수화물의 함량이 80.9%로 가장 많았고, Na, K, Ca, Fe의 무기질 4종도 비교적 풍부하게 함유되어 있었으나 비타민 B$_1$, B$_2$, C와 같은 수용성비타민은 검출되지 않았다. 가장 바람직한 추출조건은 5% 농도의 원료를 10$0^{\circ}C$의 온도에서 3시간 이내에 추출하는 것으로 나타났다. 추출물의 청정화를 위해서 depth filter pad를 이용한 여과법, 원심분리법 및 여과조제 첨가법 등을 적용한 결과 원심분리와depth filter pad를 이용했을 때 가장 우수한 청정효과를 나타내었고, 7주간 항온저장실험으로 탁도를 비교한 결과에서도 청정도의 변화가 거의 없었다. 결론적으로 상황버섯 추출물은 6,000 rpm이상에서의 원심분리나 8$\mu\textrm{m}$ 이하의 pore size를 지닌 filter system을 이용할 때 청정도를 가장 효과적으로 유지할 수 있었다

• 한국약용작물학회지
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• 제19권1호
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• pp.31-37
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• 2011

Previous work demonstrated that an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix markedly inhibits $A{\beta}$ (25-35)-induced neurotoxicity. The present study was performed to further verify the neuroprotective effect of HS0608 on oxidative and ischemic cerebral injury using cultured rat cortical neurons and rats. Exposure of cultured cortical neurons to $100\;{\mu}M$ hydrogen peroxide ($H_2O_2$) induced neuronal apoptotic death. At $10-100{\mu}g/ml$, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) induced by $H_2O_2$ in primary cultures of rat cortical neurons. In vivo, HS0608 prevented cerebral ischemic injury induced by 2-h middle cerebral artery occlusion (MCAO) and 24-h reperfusion. The ischemic infarct and edema were significantly reduced in rats that received HS0608 (200 mg/kg). These results suggest that the anti-oxidative properties of HS0608 may be responsible for its neuroprotective effect against focal cerebral ischemic injury and that HS0608 may have a therapeutic role in neurodegenerative diseases such as stroke.

• 대한한방종양학회지
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• 제8권1호
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• pp.93-101
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• 2002

Objectives This experimental study was carried out to evaluate the effects of Phellinus Linteus on immune activation. Methods In order to investigate the effect of Phellinus Linteus, the following was performed: The fraction of $CD4^+$, $CD8^+$, $CD19^+$ in splenic cell, gene expression of IL-12 (p35), IL-12 (p40) , $IFN-{\gamma}$, and splenic cell proliferation by PL-E. Results PL-E helped $CD4^+$, $CD8^+$, $CD19^+$ expression more effectively compared with control group. PL-E helped IL-12 (p35), IL-12 (p40), $IFN-{\gamma}$ gene expression in splenic cells more effectively compared with control group. PL-E proliferated splenic cells more effectively compared with control group. Conclusions: It is suggested that PL-E is able to activate immune response system.

• 한국약용작물학회지
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• 제17권6호
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• pp.388-396
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• 2009

The present study investigated an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix for possible neuroprotective effects on neurotoxicity induced by amyloid $\beta$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to $10\;{\mu}M$ $A{\beta}$ (25-35) for 36 h induced neuronal apoptotic death. At $1-50\;{\mu}g/m{\ell}$, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) induced by $A{\beta}$ (25-35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of ICR mice with 15 nmol $A{\beta}$ (25-35) was inhibited by chronic treatment with HS0608 (25, 50 and 100 mg/kg, p.o. for 7 days) as measured by a passive avoidance test. From these results, we suggest that the antidementia effect of HS0608 is due to its neuroprotective effect against $A{\beta}$ (25-35)-induced neurotoxicity and that HS0608 may have a therapeutic role in preventing the progression of Alzheimer's disease.

• 동의생리병리학회지
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• 제33권5호
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• pp.275-281
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• 2019

Polysaccharides produced in microorganisms and plants are known to increase the immune response in the body. We proposed analysis of beta-glucan contents of phellinus linteus fruit body (FB) and mycelium (MC) cultured in cudrania tricuspidata. Also, we examined whether fruit body and mycelium can increase the immune response in cyclophosphamide-induced immunosuppression animal models. We injected cyclophosphamide (50 mg/kg) twice to produce immunosuppression mice. Then, FB (200 mg/kg) and MC (200 mg/kg) were oral administered for 14 days. In order to confirm the immune-enhancing effect of FB and MC, we analyzed spleen weight, the number of immune cells, cytokines, and immunoglobulins levels. Cyclophosphamide decreased the weight of spleen, the number of immune cells. However, FB and MC have significantly increased the weight of spleen, the number of white blood cell, lymphocyte and monocyte. In addition, they have significantly increased immune-related cytokines (IL-2 and IFN-${\gamma}$) and immunoglobulins (IgA, IgG, IgM) levels. As a results, phellinus linteus fruit body (FB) and mycelium (MC) cultured in cudrania tricuspidata can be used as effective natural materials for immune-enhancing.