• 한국통신학회논문지
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• 제29권9A호
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• pp.991-997
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• 2004

본 논문은 HomePNA 2.0 모뎀 칩을 위한 모뎀 수신부의 구조를 제안한다. HomePNA 2.0 전송 채널은 브릿지 탭과 HAM 대역의 영향 등으로 매우 열악하다. 이러한 채널을 통해 전송을 가능하게 하기 위해 HomePNA 2.0 은 훈련신호를 사용하여 매 프레임 마다 채널을 등화하고 FD-QAM 전송 방식을 선택적으로 사용한다. 따라서 모뎀 수신부는 일반적 QAM 방식 신호의 북조 기능과 함께 이러한 전송 방식의 특정을 최대한 상려 모뎀 수신 성 능을 극대화 히는 구조가 필요하다 연구 결과 모뎀 수신부의 가능을 송수신 상태에 따라 정상 수신 모드와 충돌 감지 오드의 2 가지로 정의 하였다 본 논문은 특히, 모뎀 수신부를 구성하는 핵심 블록인 등화기와 위상 동기부, 프레임 동기부에 대해서 사용된 알고리즘을 밝혔으며, 버스트 방식 모뎀의 채널 등화 성능을 높이고 안정적으로 동작 시키기 위한 구조를 제얀 하였다 마지막으로 제안된 모뎀 수신부의 성능을 분석하기 위해서 SPW 모델을 사용하여 채널 별 전송 가능 속도를 예측 하였다.

• Journal of Microbiology
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• 제35권1호
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• pp.21-24
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• 1997

The hybrid peptides, CA-ME, CA-MA and CA-BO, with the N-terminal sequence 1-8 of cecropin A and the N-terminal sequences 1-12 of melittin, magainin 2 and bombinin, respectively, have more improved antibacterial activities. CA-MA was found to have stronger antifungal activity against Trichoderma sp than other hybrid peptides and their parental peptides. In order to elucidate the relationships between the peptide structure and antifungal activity, several analogues of CA-MA or CA-BO were also designed and synthesized by the solid phase method. An tifungal activity was measured against T. reesei and T. viride, and hemolytic activity was measured by a solution method against human red blood cells. The residue 16 of CA-MA, Ser, was found to be important for antifungal activity. When the residue was substituted with Leu, showed powerful antifungal activity was dramatically decreased. CA-MA, P1, P4 and P5 designed in this study showed powerful antifungal activity against T. reesei and T. viride with low hemolytic activity against human red blood cells. These hybrid peptides will be potentially useful model to further design peptides with powerful antifungal activity for the effective therepy of fungal infection and understand the mechanisms of antifungal actions of hybrid peptides.

• 전기전자학회논문지
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• 제20권3호
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• pp.251-259
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• 2016

본 논문에서는 순차바이어스와 스위칭 타임 튜닝기법을 통한 반도체 송수신모듈(TRM : Transmitter and Receiver Module)의 강건성 향상 방법에 대해 기술한다. 기존의 회로설계는 TRM의 소형화로 인한 송신출력신호가 수신기로 유기되어 최소수신감도(MDS : Minimum Detection Signal) 개선에 초점을 맞추어졌으나, 평균고장시간(MTBF : Mean Time Between Failure)을 만족하지 못하고 빈번히 고장이 발생하는 문제가 있었다. 본 연구는 이러한 현상을 개선하는 방법으로 순차바이어스 및 스위칭 타임 튜닝기법을 제안한다. 첫 번째로 주요 고장증상 수집 및 원인을 추론하였으며, 두 번째로 개선방법을 도출하고 시스템에 적용하여 효과를 검증하였다. 제안한 방법을 적용하여 격리도 부족에 따른 빈번한 고장증상이 해소되었다.

• Journal of Pharmaceutical Investigation
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• 제35권5호
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• pp.323-328
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• 2005

The purpose of the present study was to evaluate the bioequivalence of two torasemide tablets, Torem tablet (Roche Korea Co., Ltd., Korea, reference drug) and Boryung Torsemide tablet (Boryung Pharmaceutical Co., Ltd., Korea, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). After adding an internal standard (furosemide) to human serum, serum samples were extracted using 5 mL of ethyl acetate. Compounds were analyzed by reverse-phase HPLC method with UV detection. This method showed linear response over the concentration range of 0.05 ug/mL with correlation coefficient of 0.999. The lower limit of quantitation using 0.5 mL of serum was 0.05 ug/mL which was sensitive enough for pharmacokinetic studies. Twenty-eight healthy male Korean volunteers received each medicine at the torasemide dose of 20 mg in a $2{\times}2$ crossover study. There was a one-week washout period between the doses. Serum concentrations of torasemide were monitored by an HPLC-UV for over a period of 12 hr after the administration. $AUC_{t}$(the area under the serum concentration-time curve from time zero to 12 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (maximum serum drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the serum concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_{t}$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_{t}$ ratio and the $C_{max}$ ratio for Boryung Torsemide/Torem were log 0.97-10g 1.03 and log 0.93log 1.12, respectively. These values were within the acceptable bioequivalence intervals of log 0.80-log 1.25. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Boryung Torsemide tablet and Torem tablet are bioequivalent.

• Genomics & Informatics
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• 제16권2호
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• pp.22-29
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• 2018

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 strains) covering 88.5% of all 4,919 open reading frames, 7,734 barcodes (88.8%) were validated as high-fidelity to be inserted at the correct positions by Sanger sequencing. Sequence examination of the 7,734 high-fidelity barcodes revealed that 1,039 barcodes (13.4%) deviated from the original design. In total, 1,284 mutations (mutation rate of 16.6%) exist within the 1,039 mutated barcodes, which is comparable to budding yeast (18%). When the type of mutation was considered, substitutions accounted for 845 mutations (10.9%), deletions accounted for 319 mutations (4.1%), and insertions accounted for 121 mutations (1.6%). Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. When the mutation rate was analyzed by position within 20-mer barcodes using the 1,284 mutations from the 7,734 sequenced barcodes, there was no significant difference between up-tags and down-tags at a given position. The mutation frequency at a given position was similar at most positions, ranging from 0.4% (32/7,734) to 1.1% (82/7,734), except at position 1, which was highest (3.1%), as in budding yeast. Together, well-defined barcode sequences, combined with the next-generation sequencing platform, promise to make the fission yeast gene deletion library a powerful tool for understanding gene function.

• 생명과학회지
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• 제31권1호
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• pp.83-89
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• 2021

개불 라이소자임(Uu-ilys)은 개불(Urechis unicinctus)로부터 정제된 무척추형 라이소자임으로 병원균에 대한 방어에 주요하게 작용하는 선천성 면역 물질이며, 비효소적 항균 활성을 가지고 있어 항균 활성을 지닌 유도체의 개발 가능성을 가지고 있다. 본 논문은 개불 라이소자임에서 유래된 항균 활성을 가지는 유도체의 디자인과 생산을 기술하고 있다. 여러 항균성 펩타이드(antimicrobial peptide, AMP) 데이터베이스에서 제공하는 항균성 펩타이드 예측 도구를 사용하여 개불 라이소자임에서 항균 활성을 가지는 부위를 예측하였다. 개불 라이소자임 C-말단의 절편이 항균 활성을 나타낼 것으로 예측되었으며, 이 펩타이드는 C-말단 절편, Uu-ilys-CF라 명명하였다. Uu-ilys-CF은 이형 발현 시스템인 TrxA-Uu-ilys-CF 퓨전 단백질을 사용하여 생산하였다. 만들어진 퓨전 단백질은 브롬화시안을 사용하여 메티오닌 잔기를 절단하였으며, 절단된 Uu-ilys-CF은 고성능액체크로마토그래피와 역상 컬럼인 CapCell-Pak C18을 사용하여 분리되었다. Uu-ilys-CF의 항균 활성을 조사하기 위해서 여러 균주(그람양성균 4개, 그람음성균7개, 진균 1개)를 사용하였다. Uu-ilys-CF의 항균 활성은 살모넬라에서 가장 높은 반응을 보였다. 비록 Uu-ilys-CF는 진균에 활성을 나타내지 않았지만, 사용한 균주들에서 넓은 범위의 항균 활성을 나타내었다.