• Journal of Applied Biological Chemistry
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• v.59 no.4
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• pp.313-316
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• 2016

Sparassis crispa fruits were extracted in 80 % MeOH, and the concentrated extract was partitioned into EtOAc, n-butyl alcohol, and water fractions. The repeated octadecyl $SiO_2$ and silica gel ($SiO_2$) column chromatographies for the EtOAc and nbutyl alcohol fractions led to isolation of two ergosterol peroxides. There chemical structures were determined as ($3{\beta}$,$5{\alpha}$,$8{\alpha}$,22E)-5,8-Epidioxyergosta-6,22-dien-3-ol (ergosterol peroxide) (1) and 3-O-${\beta}$-D-glucopyranosyl ergosterol peroxide (2) based on spectroscopic data analyses including nuclear magnetic resonance, infrared spectrometry, and mass spectrometry (MS). Compounds 1 and 2 were for the first time isolated from S. crispa in this study.

• The Journal of Natural Sciences
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• v.14 no.1
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• pp.7-24
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• 2004

We found new type of thiol peroxidase, fused with GRX.(TPx-GRX) The TPx-GRX exists in pathogenic bacteria including -. This protein was homogeneously purified from the E.coli recombinant overexpressing TPx-GRX. In the presence of a thiol-containing electron donor such as DTT, the purified TPx-GRX has potent the antioxidant to prevent the inactivation of GS by the MCO system, which is comprised of DTT, $Fe^{3+}$, and $O^2$. The antioxidant activity is much higher that other thiol peroxidase. The investigate the peroxidase activity of TPx-GRX, we directly measured the peroxidase activity of TPx-GRX toward peroxides in terms of the removal of peroxides in the presence of GSH. This result demonstrates that the peroxidase activity of TPx-GRX. These taken together results suggest that TPx-GRX is a new member of thiol peroxidase. These observations also suggest that in the pathogenic bacteria, TPx-GRX plays an important antioxidative role as a multiple array defence mechanism against oxidative stress.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.2
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• pp.150-157
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• 2006

The aim of the present study was to investigate the effect of combined extract of safflower seed with herbs on the improvement of blood glucose, lipid peroxides, lipids in the plasma and liver of strpetozotocin-induced diabetic rats. Rats in the experimental group were orally administered with combined extract of safflower seed (100 mg, 200 mg/kg B.W.) with herbs (Ophiopogon japonicus Ker-Gaqler, Glycyrrhiza uralensis Fisch, Mori Folium, Poria cocos, Rehmannia glutinosa, Eriobtrya japonica, Aralia continentalis Kitagawa, Zizyphus jujuba var, Cornus officinalis, Paeonia suffruticosa, Trichosanthes kirilowii Maxim and Schizandra chinensis Baill) for 4 weeks. Body weight gain and food efficiency ratio were significantly lower in diabetic groups than those of control group. These were no protective effect of the supplementation of combined extract of safflower seed with herbs. Concentration of blood glucose was significantly higher in the diabetic groups than those in the control group. Blood glucose concentration was remarkably lower supplementation of combined extract of safflower seed (200 mg/kg B.W.) with herbs. There was no significant difference of plasma lipid peroxides among experimental groups, while liver lipid peroxides of diabetic group was significantly higher in control group. But supplementation of combined extract of safflower seed with herbs was induced markedly lower in liver lipid peroxides in diabetic rats. Diabetic groups had markedly higher levels in triglycerides, LDL-cholesterol and atherogenic index, while had lower HDL-cholesterol level. Triglyceride levels of plasma and liver were significantly lower with combined extract of safflower seed with herbs. But total cholesterol, phospholipid and free fatty acid were no differing significantly among experimental groups.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.6
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• pp.1047-1052
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• 1998

The effects of antioxidants such as ascorbyl palmitate(AP), tocopherol( Toc), BHA and ascorbyl palmitate＋ tocopherol(AP＋ Toc) on lipid peroxide formations in lard have been investigated during storage at 50oC after gamma irradiation with the dose of 1~10kGy. Immediately gamma irradiation greatly increased the initiative oxidation of lard as expected. But antioxidants were found to be greatly effective in minimizing the radiation induced peroxidation of lard. The decreasing order of effects on their antioxidative activities was AP>AP＋ Toc>BHA> Toc. Oxidation of lard during storage at 50oC after irradiation induced the acceleration of autooxidation. But the additions of an tioxidants inhibited the formation of peroxides. Their antioxidative activities during storage were BHA> AP＋ Toc> Toc>AP. Especially AP＋ Toc mixture was not significantly different from BHA (p>0.05).

• BMB Reports
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• v.44 no.8
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• pp.497-505
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• 2011

Reactive oxygen species (ROS), which include superoxide anions and peroxides, induce oxidative stress, contributing to the initiation and progression of cardiovascular diseases involving atherosclerosis. The endogenous and exogenous factors hypercholesterolemia, hyperglycemia, hypertension, and shear stress induce various enzyme systems such as nicotinamide adenine dinucleotide (phosphate) oxidase, xanthine oxidase, and lipoxygenase in vascular and immune cells, which generate ROS. Besides inducing oxidative stress, ROS mediate signaling pathways involved in monocyte adhesion and infiltration, platelet activation, and smooth muscle cell migration. A number of antioxidant enzymes (e.g., superoxide dismutases, catalase, glutathione peroxidases, and peroxiredoxins) regulate ROS in vascular and immune cells. Atherosclerosis results from a local imbalance between ROS production and these antioxidant enzymes. In this review, we will discuss 1) oxidative stress and atherosclerosis, 2) ROS-dependent atherogenic signaling in endothelial cells, macrophages, and vascular smooth muscle cells, 3) roles of peroxidases in atherosclerosis, and 4) antioxidant drugs and therapeutic perspectives.

• Textile Coloration and Finishing
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• v.5 no.1
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• pp.26-32
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• 1993

This study is concerned with the graft polymerization of acrylamide onto the surfaces of polyethylene and polyethylene terephthalate films treated with on corona discharge. In the case, peroxides formed by the corona discharge treatment are likely to be the species responsible for initiating the graft polymerization. This treatment produced a continuous charge in wettability and also amid group density on the polymer surface, as evidenced by water contact angle measurement, Fourier-transform infrared spectroscopy in the attenuated total reflectance mode, and electron spectroscopy for chemical analysis. Both of the merely corona-treated film and the subsequently grafted film are discussed as a function of time after treatment and water washings.

• BMB Reports
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• v.32 no.5
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• pp.506-510
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• 1999

The newly-found thiol peroxidases (TPx) with a conserved cysteine as the primary site of catalysis are capable of catalyzing the thiol-dependent reduction of peroxides. However, the cellular distributions of the isoforms remain poorly understood. As a first step in understanding the physiological functions of the TPx isoforms, we examined the cellular and tissue distribution of the isoenzymes in various bovine tissues. The tissue distributions of TPx isoenzymes indicate that two types of TPx are widely distributed throughout all of the tested tissues. These two forms are the predominant proteins, with levels of the proteins being quite different from each other. The level of predominant TPx proteins, named type II (TPx II) and type V (TPx V), appeared to be very different with respect to tissue type. The cellular distribution and level of TPx isoenzymes also varied with the types of cells. Immunoblot analysis of the mitochondrial and cytosol fractions from various tissues indicates that TPx III is a unique mitochondrial form. Based on the different tissue and cellular distribution of TPx isoenzymes, we discuss the physiological function of TPx isoenzymes, especially the ubiquitous TPx II.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.2
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• pp.221-224
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• 1997

Effects of melatonin on hepatic glutathione-peroxidase (GSH-Px) and glutathione-reductase (GSH-reductase) activities were studied in Sprague-Dawley (SD) rats administered i.p. (10 mg/kg body weight) with melatonin during 15 days. The activity of cytosolic GSH-reductase in the liver was not changed by melatonin. However, melatonin injection increased significantly the activity of liver cytosolic GSH-Px activity compared with those in saline-treated rats. At the same time, plasma GSH-Px was also increased significantly in melatonin-treated rats. Since GSH-Px, a major antioxidative enzyme, removes $H_2O_2$ and lipid peroxides which are formed during lipid peroxidation from cellular membrane, such elevation of heptatic GSH-Px activity may contribute to the improvement of antioxidative effects under oxidative damage in the liver.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.47-53
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• 2013

Four new cyclic peroxide compounds (1~4) were isolated from the marine sponge Plakortis simplex. Their structures including relative stereochemistry were determined by MS and NMR analysis. All compounds, a side carbon chain with 10 carbons, were very unstable. After transformation into methyl ester analogues, the structure determination was conducted. Compounds 1a and 2a are stereoisomers, assigned as $3S^*$, $4S^*$, $6R^*$ and $3R^*$, $4S^*$, $6R^*$, respectively. Similarly, compounds 3a and 4a, replaced the methoxy group with an aliphatic methyl, are also stereoisomers. Compounds 1a and 2a exhibited the strong antifungal effect against the fungus Candida albicans.

• Environmental Analysis Health and Toxicology
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• v.15 no.1_2
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• pp.7-12
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• 2000

Scavenging effects on paraquat induced toxicity were investigated by using methanol (MeOH) and ethylacetate (EtoAC) extracts of Lonicera japonica. The results are summerized as follows: 1. To Fe(III)-ADP-NADPH induced microsomal lipid peroixdation, MeOH and EtoAC extracts showed antioxidative activiies and inhibition ratio at 100 $\mu\textrm{g}$/$m\ell$ 44.4% and 73.8% respectively 2. To microsomal NADPH dependent cytochrome p -450 reductase in rat liver, MeOH and EtoAC extracts inhibited the enzyme activiies and inhibition ratio were 26.3% and 44.8% respectively. 3. Administration (30 mg/kg, iv) of paraquat to rats caused the marked elevation of GOT, GPT, LDH, ALP in the serum and lipid peroxides in the microsome as compared to the control group. Serum GTP, LDH, ALP and liver microsomal LPO were reduced significantaly by administration of MeOH extract. (1,000 mg/kg), EtoAC extract (40 mg/kg) and Silymarin (150 mg/kg) as compared to the paraquat group. From the results, MeOH and EtoAc exuacts. of Lonicera japonica showed the useful scavenger and reducer on the paraquat induced hepatotoxicty.