• 사상체질의학회지
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• 제11권2호
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• pp.251-266
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• 1999

1. 연구목적 본 실험은 청심연자탕이 대뇌신경세포의 산화적 손상에 대한 효능을 밝히기 위한 것이다. 2. 연구방법 신생 생쥐에서 분리 배양한 대뇌신경세포에 여러 농도의 hydrogen peroxide가 포함된 배양액에서 6시간 동안 처리하여 hydrogen peroxide가 배양 대뇌신경세포에 미치는 영향을 조사하였으며 hydrogen peroxide의 독성효과에 대한 청심연자당의 영향을 조사하였다. 3. 결과 및 결론 산소자유기인 hydrogen peroxide는 NR assay와 MTT assay에 의한 세포생존율을 감소시켰고 lipid peroxidation의 증가 및 LDH양의 증가에 의하여 생쥐의 배양 대뇌신경세포에 독성을 나타냈다. 청심연자탕(淸心蓮子湯)은 hydrogen peroxide의 산화적 손상에 대한 신경독성에 대하여 Iipid peroxidation의 감소에 유의한 효과를 보였다. 청심연자탕(淸心蓮子湯)은 hydrogen peroxide의 산화적 손상에 의한 신경독성에 대하여 LDH양의 감소에 유의한 효과를 보였다. 이상의 결과로 보아 hydrogen peroxide는 생쥐에서 분리한 대뇌신경세포에 산화적 손상에 의한 신경독성을 나타냈으며 청심연자탕(淸心蓮子湯)이 hydrogen peroxide와 같은 산소자유기의 산화적 손상에 대한 방어에 효과적인 것으로 사료된다.

• 약학회지
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• 제28권6호
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• pp.299-303
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• 1984

In order to eluciate the effect of humidity and organic solvent on the decomposition of carbamide peroxide, the kinetic study was carried out. The carbamide peroxide was prepared from urea and 30%-hydrogen peroxide. The accelerated stability analysis for carbamide peroxide crystal in various relative humidity, and for 10%-carbamide peroxide solution of organic solvents were investigated. Both humidity and temperature were important factors influencing the decomposition rate of carbamide peroxide crystal. The higher the humidity and temperature, the greater was the reaction rate. The breakdown rate of crystal was observed as an apparent zero-order, and was faster than the rate of decomposition in dilute propylene glycol, glycerine or sorbitol solutioos which were measured as an apparent first-order reaction. The more dilute to 10% the organic solvents of 10%-carbamide peroxide, the slower was breakdown rate. It is, therefore, useful in the aspects of stability and economics to substitute solvent of carbamide peroxide topical solution (USP XXI) with 10%-propylene glycol or glycerine instead of anhydrous glycerine.

• 한국추진공학회지
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• 제13권4호
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• pp.16-21
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• 2009

과산화수소의 저장성 평가 방법을 소개하고, 고농도 과산화수소의 저장성을 평가하였다. 농도 측정 방법을 통하여 저장성을 평가하였으며, 과산화수소의 MIL-PRF-16005F 기준 만족 여부와 저장 온도 및 저장 용기의 마개 재질을 실험 변수로 선정하여 저장성을 평가하였다. 8~24개월 동안 측정을 수행한 결과, 안정제가 포함된 과산화수소가 추진제급 과산화수소에 비해 저장성이 매우 떨어졌으며, 과산화수소 증기와 반응을 보이지 않은 파라핀 필름으로 용기를 밀폐하였을 때, 플라스틱 마개를 사용한 경우에 비해 좋은 저장성을 나타내었다. 또한 과산화수소의 저장성에는 온도가 매우 큰 영향을 미쳐 상온에 비해 $10^{\circ}C$ 이하에서 보관하였을 때 저장성이 크게 향상되었다.

• Journal of Microbiology
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• 제38권2호
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• pp.99-104
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• 2000

Hydrogen peroxide has been used in cleaning the piping of an advanced high-purity water system that supplies ultra-high purity water (UHPW) for 16 megabyte DRAM semiconductor manufacturing. The level of hydrogen peroxide-resistant bacteria in UHPW water was monitored prior to and after disinfecting the piping with hydrogen peroxide. Most of the bacteria isolated after hydrogen peroxide disinfection were highly resistant to hydrogen peroxide. However, the percentage of resistant bacteria decreased with time. The hydrogen peroxide-resistant bacteria were identified as Micrococcus luteus, Bacillus cereus, Alcaligenes latus, Xanthomonas sp. and Flavobacterium indologenes. The susceptibility of the bacteria to hydrogen peroxide was tested as either planktonic cells or attached cells on glass. Attached bacteria as the biofilm on glass exhibited increased hydrogen peroxide resistnace, with the resistance increasing with respect to the age of the biofilm regrowth on piping after hydrogen peroxide treatment. In order to optimize the cleaning strategy for piping of the high-purity water system, the disinfecting effect of hydrogen preoxide and peracetic acid on the bacteria was evaluated. The combined use of hydrogen peroxide and peracetic acid was very effective in killing attached bacteria as well as planktonic bacteria.

• 대한치과기공학회지
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• 제23권1호
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• pp.85-93
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• 2001

The purpose of this study was to evaluate the effects of commercial home-tooth bleaching agents on the color of tooth. Twenty five sound extracted teeth were randomly divided into five groups. The color differences between before and after treatment with five types of tooth bleaching agents (7.5% hydrogen peroxide Nite White $Excel^{(R)}$, 10% carbamide peroxide Nite White $Excel^{(R)}$, 16% carbamide peroxide Nite White $Excel^{(R)}$, 10% carbamide peroxide Insta-BriteTM, 20% carbamide peroxide Insta-$Brite^{TM}$) were evaluated. The results were as follows: 1. By 2 week home tooth bleaching agent applications, the values ($L^*$) of bovine teeth increased as high as 4.38 $\sim$ 8.80 when comparing to those of the samples before treatment, and the color difference (${\Delta}E^*$) showed as high as 10.16 $\sim$ 15.04. 2. 16% carbamide peroxide Nite White Excel induced significantly greater ${\Delta}L^*$ than other test edgroups except for 7.5% hydrogen peroxide Day White Excel, and significantly greater ${\Delta}E^*$ than other tested groups by 2 week bleaching agent treatments (p<0.01). 3. 16% carbamide peroxide Nite White Excel(${\Delta}L^*$=8.80, ${\Delta}E^*$=15.04) induced significantly greater ${\Delta}L^*$ and ${\Delta}E^*$ than 10% carbamide peroxide Nite White Excel(${\Delta}L^*$=5.01, ${\Delta}E^*$=10.16)(p<0.01), but significant difference between 10% carbamide peroxide Insta-Brite(${\Delta}L^*$=4.38, ${\Delta}E^*$=10.51) and 20% carbamide peroxide Insta-Brite(${\Delta}L^*$=5.63, ${\Delta}E^*$=11.23) was not shown in ${\Delta}L^*$ and ${\Delta}E^*$(p>0.01). 4. 16% carbamide peroxide Nite White Excel(${\Delta}L^*$=8.80, ${\Delta}E^*$=15.04) which were applied in night time induced significantly greater ${\Delta}L^*$ and ${\Delta}E^*$ than 7.5% hydrogen peroxide Day White Excel(${\Delta}L^*$=8.47, ${\Delta}E^*$=12.75) which were applied in day time. Conclusions: These results demonstrate that all the commercial home-tooth bleaching agents have appreciable bleaching effect on teeth, and the effects of home-tooth bleaching agents which are used during night time are affected by content of carbamide peroxide. Especially the whitening effect of home tooth bleaching agents that are used through night time is greater than that of short time-applying tooth bleaching agent.

• Bulletin of the Korean Chemical Society
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• 제32권7호
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• pp.2187-2192
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• 2011

Hydrogen peroxide plays a key role as a second messenger in the normal cellular signaling but its overproduction has been implicated in various life-threatening diseases. Peroxalate chemiluminescence is the light emission from a three component reaction between peroxalate, hydrogen peroxide and fluorophores. It has proven great potential as a methodology to detect hydrogen peroxide in physiological environments because of its excellent sensitivity and specificity to hydrogen peroxide. We developed chemiluminescent micelles composed of amphiphilic polymers, peroxalate and fluorescent dyes to detect hydrogen peroxide at physiological concentrations. In this work, we studied the relationship between the chemiluminescence reactivity and stability of peroxalate by varying the substitutes on the aryl rings of peroxalate. Alkyl substitutes on the aryl ring of peroxalate increased the stability against water hydrolysis, but diminished the reactivity to hydrogen peroxide. Chemiluminescent micelles encapsulating diphenyl peroxalate showed significantly higher chemiluminescence intensity than the counterpart encapsulating dimethylphenyl or dipropylphenyl peroxalate. Diphenyl peroxalate-encapsulated micelles could detect hydrogen peroxide generated from macrophage cells stimulated by lipopolysaccharide (LPS) and image hydrogen peroxide generated during LPS-induced inflammatory responses in a mouse.

• 대한본초학회지
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• 제26권1호
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• pp.53-58
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• 2011

Objectives : The purpose of this study is to investigate effects of Scutellariae Radix Water Extract on hydrogen peroxide production in RAW 264.7 mouse macrophages. Methods : Scutellariae Radix produced from South Korea (SK) and Scutellariae Radix produced from China (SC) were extracted by hot water. Effects of SK and SC on hydrogen peroxide production in RAW 264.7 were measured by dihydrorhodamine 123 assay after 2, 4, 20, 24, 28, 44, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL. Results : SK significantly increase hydrogen peroxide production in RAW 264.7 cells for 2, 4, 20, 24, 28, 44, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). SC also significantly increase hydrogen peroxide production in RAW 264.7 cells for 4, 20, 24, 28, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). For 2 h incubation, SC significantly increase hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, and 100 ug/mL (P < 0.05). For 44 h incubation, SC significantly increase hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, and 50 ug/mL (P < 0.05). Conclusions : These results suggest that Scutellariae Radix has the immune - enhancing property related with its increasement of hydrogen peroxide production in macrophages.

• 약학회지
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• 제46권5호
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• pp.324-330
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• 2002

This experiment was carried out to determine non-destructively the hydrogen peroxide concentration of 3％ antiseptic hydrogen peroxide solutions by portable near-infrared (NIR) system. Hydrogen peroxide standards were prepared ranging from 0 to 25.6 w/w％ and the NIR spectra of hydrogen peroxide standard solutions were collected by using a quartz cell in 1 mm pathlength. We found the variation of absorbance band due to OH vibration of hydrogen peroxide depending on the concentration around 1400 nm in the second derivatives spectra. Partial least square regression (PLSR) and multilinear regression (MLR) were explored to develop a calibration model over the spectral range 1100-1720 nm. The model using PLSR was better than that using MLR. The calibration showed good results with a standard error of prediction (SEP) of 0.16％. In order to validate the developed calibration model, routine analyses were performed using commercial antiseptic hydrogen peroxide solutions. The hydrogen peroxide values from the NIR calibration model were compared with the values from a redox titration method. The NIR routine analyses results showed good correlation with those of the redox titration method. This study showed that the rapid and non-destructive determination of hydrogen peroxide in the antiseptic solution was successfully performed by portable NIR system without very harmful solvents.

• 대한수의학회지
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• 제38권2호
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• pp.273-279
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• 1998

The ability of bovine intact red blood cells to scavenge superoxide and hydrogen peroxide by superoxide dismutase, catalase and glutathione peroxidase was investigated. Intact red cells(up to 0.4%) suspensions did not inhibit ferricytochrome c reduction by superoxide in the superoxide generating system. On the other hand, intact red cell(0.4%) suspensions almost completely inhibit ferrocytochrome c oxidation by hydrogen peroxide. The ability of intact red cells to scavenge hydrogen peroxide was mainly attributed to either membrane bound catalase or glutathione peroxidase. The scavenge of hydrogen peroxide by 0.1~0.2% intact red cells showed a trend of dependence on mainly glutathione peroxidase. However, at blood cell concentration higher than 0.3%, the process depended upon peroxidase-independent scavengers like catalase. Enhancement of ferrocytochrome c oxidation by red cells treated with aminotriazole proved that the protection against hydrogen peroxide was due to catalase, while the protection in the presence of glutathione indicated scavenging effect of glutathione peroxidase against hydrogen peroxide.

• 펄프종이기술
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• 제38권3호
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• pp.79-84
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• 2006

Hydrogen peroxide has been a bleaching chemical for varied pulp, especially mechanical and deinking pulp. It is catalytically decomposed by some transition metals in pulp slurry. In this paper, some metals which can be contained in pulp such as manganese, copper, iron, magnesium and calcium were used to investigate their effect on the decomposition of hydrogen peroxide. From the result, hydrogen peroxide was more decomposed in the order of Mn, Cu, $Fe^{3+}\;and\;Fe^{2+}$, while Mg and Ca had little effect on the decomposition of hydrogen peroxide. The effect of Mg/Mn ratio on the decomposition of hydrogen peroxide was also investigated. At the specific ratio of them(Mg/Mn=10), hindering effect of peroxide decomposition by Mg was decreased.