• 대한한방소아과학회지
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• 제18권2호
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• pp.61-76
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• 2004

Objective : To demonstrate of regulatory effect of atopic allergic regulation by Chungsangbangpoong-Tang(CBT), This experiment was studied. Methods : The author investigated a possible effect of CBT on cytokines production using human T cell line (MOLT-4) or human mast cell line (HMC-1). In addition, the author investigated whether CBT has inhitory effects on compound 48/80- induced histamine release from rat peritoneal mast cells (RPMC) and compound 48/80-induced ear swelling in ICR mice. Results : CBT (0.01 mg/ml)-containing medium in stimulated culture supernatants significantly increased IL-2 secretion compared with untreated MOLT-4, whereas CBT (0.01-1.0 mg/ml)-containing medium in stimulated culture supernatants significantly decreased IL-4 secretion compared with untreated MOLT-4. Significant reduced levels of IL -6 and $TNF-{\alpha}$ were observed in the HMC-l with CBT (P<0.05). CBT did not inhibit the histamine release from the RPMC but inhibit ear swelling response. Conclusion : These results suggest that CBT contributes to the treatment of atopic allergic reactions, such as atopic dermatitis and that its action may be due to regulation of cytokine production.

• 대한한방부인과학회지
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• 제27권1호
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• pp.101-119
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• 2014

Objectives: This study was performed to investigate the effects of Hominis Placenta pharmacopuncture (HPP) therapy on the experimentally-induced endometriosis in the rats. Materials and Methods: Endometriosis was induced in rats by autotransplanting uterine tissue to the peritoneum and divided them into three groups: (1) sham-operated group (n=8), (2) surgically induced endometriosis and untreated control group (n=8), (3) surgically induced endometriosis and HPP treated group. Sham-operated group and control group were inject with normal saline once a every other day for 30days, while treated group was injected with HPP extract once a every other day for same duration. Injected point of HPP and normal saline were subcutaneous tissue at Gwanwon (CV4) acupoint. Then we measured the body weight, the volume of endometriotic implants, the weigh of uterus and ovaries, and investigated the concentration of cytokines (MCP-1, TNF-${\alpha}$) in peritoneal fluids. Histopathology, immunohistochemisty for COX-2 and VEGF, and histochemistry for mast cell in transplanted uterine tissue were performed. Results: The volume ($mm^2$) of endometriotic implants in HPP treated group ($55.4{\pm}41.6$) was significantly decreased (p<0.01) compared with control group ($140{\pm}66.1$). And the concentration (pg/ml) of MCP-1 in peritoneal fluids in HPP treated group ($1117.6{\pm}60.5$) was significantly decreased (p<0.01) compared with control group ($1446.2{\pm}280.3$). The concentration (pg/ml) of TNF-${\alpha}$ in peritoneal fluids in HPP treated group ($80.6{\pm}31.4$) was decreased (p<0.01) compared with control group ($145.3{\pm}86.9$). Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatory cell and angiogenesis in transplanted uterine tissue of HPP treated group were weakly observed than those of control group. The COX-2 expression in endometrial, epithelial and stromal cells in transplanted uterine tissue of HPP treated group was decreased compared with control group. The VEGF expression of endometriotic epithelia, neovascular endothelia and stromal cell in transplanted uterine tissue of HPP treated group were weakly observed than those of control goup. Conclusions: HPP is effect on Endometriosis of rats by Experimentally-induced.

• IMMUNE NETWORK
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• 제2권4호
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• pp.195-201
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• 2002

Background: IgE is closely related to the development of allergies. However, the poor relationship between the specific IgE level and the severity of allergic diseases suggests the possibility of functionally different IgE isoforms. With this in mind, rat basophilic leukemia (RBL)-2H3 activation was analyzed with each type of rat IgE for two parameters, exocytosis and IL-4 mRNA production. RBL-2H3 has been well documented in the rat mucosal mast cell line. Methods: RBL-2H3 cells sensitized with each kind of rat IgE was activated by cross-linking FcRI with B5 (monoclonal anti-rat IgE mouse IgG antibodies). The RBL-2H3 exocytosis was measured by analyzing the ${\beta}$-hexosaminidase level, and the level of IL-4 mRNA synthesis was analyzed using semiquantitative RT-PCR. Rat IgE, which was produced by a parasite infection (REP), was prepared using either Paragonimus westermani metacercariae (REP-PW) or Anisakis simplex third stage larvae (REP-AS). A rat IgE prototype of IR162 was prepared by a peritoneal injection of immunocytoma. Results: The level of exocytosis showed a linear relationship with the rat IgE concentration when REP-PW or REP-AS was applied. However, it exhibited a biphasic response with IR162. In addition, the time course of heating at $56^{\circ}C$ illustrated the similarity between REP-PW and REP-AS, which differed from that of IR162. In contrast, the level of IL-4 mRNA synthesis in the RBL-2H3 cells with IR162 was comparable to that of either REP-PW or REP-AS. Conclusion: These results suggest that functionally different rat IgE isoforms exists in RBL-2H3 exocytosis.

• 한국식품영양과학회지
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• 제36권2호
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• pp.155-162
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• 2007

본 연구에서는 녹차(Camellia sinensis)잎 추출물, 구아바(Psidium guajava)잎 추출물 그리고 장미(Rosa hybrida)꽃잎 추출물로 이루어진 식물추출 복합물(PEM381)에 대하여 제I형 알레르기 반응 억제 효능 및 생화학적 기전을 밝히기 위하여 COX 억제 활성, LO 억제 활성, compound 48/80에 의한 랫드 복강 비만세포의 탈과립과 히스타민 유리에 미치는 영향 그리고 수동피부아나필락시스 반응 억제효능을 측정하였다. 그 결과 PEM381은 5-LO $IC_{50}$ 값이 $14.11{\pm}0.51ppm$으로 나타났고, COX-1에 비해 COX-2를 선택적으로 억제하는 것으로 나타났다. 또한 PEM381은 compound 48/80에 의한 비만세포의 탈과립과 히스타민 유리를 농도 의존적으로 억제할 뿐만 아니라 수동피부아나필락시스 반응도 억제하였다. 이상의 결과로 PEM381은 알레르기성 비염, 아토피 피부염 및 기관지천식 같은 제I형 알레르기 질환에 대하여 예방 및 치료에 유용하게 사용될 수 있을 것으로 생각된다.

• Natural Product Sciences
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• 제9권1호
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• pp.44-48
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• 2003

The effect of aqueous extract of Stachys riederi var. japonica Miq. (Labiatae) (SRAE) on the immediate-type allergic reactions was investigated. SRAE was found to exhibit a inhibitory activity on the compound 48/80-induced systemic anaphylaxis in mice. SRAE inhibited the plasma histamine release induced by compound 48/80 in mice. In addition, SRAE also inhibited the passive cutaneous anaphylaxis reaction induced by IgE/anti-IgE in mice. The effect of SRAE on the histamine release from rat peritoneal mast cells (RPMC) was studied. SRAE inhibited the histamine release induced by compound 48/80 in RPMC. To clarify the mechanism of these inhibiting reactions, we investigated the effects of SRAE on cyclic AMP (cAMP). The level of cAMP in human leukemia cell line, HMC-1, when SRAE (1 mg/ml) was added, significantly increased compared with that of basal cells. These results indicate that SRAE may be beneficial in the treatment of immediate-type allergic reaction.

• 대한한방부인과학회지
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• 제22권3호
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• pp.1-24
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• 2009

Purpose: This study was performed to investigate the effects of Bojungikgi-Tang on the development of experimentally-induced endometriosis in rats. Methods: Endometriosis was induced in rats by autotransplanting uterine tissue to the peritoneum and we divided the rats into three groups: (1) sham-operated group (n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3) surgically induced endometriosis and Bojungikgi-Tang treated group. Bojungikgi-Tang was orally administrated for 15 days after operation. Then we measured the body weight, the volume of endometriotic implants, the weight of uterus and ovary, and analysed the concentration of cytokines(MCP-1, TNF-${\alpha}$, IL-1${\beta}$, IL-6) in peritoneal fluids. Histopathology, immunohistochemistry for COX-2 and VEGF, and histochemistry for mast cell in transplanted uterine tissue were also performed. Results: 1. The volume($mm^3$) of endometriotic implants in Bojungikgi-Tang treated group(129${\pm}$39) was significantly decreased(p<0.05) compared with control group(222${\pm}$109). 2. The concentration(pg/$m{\ell}$) of MCP-1 in peritoneal fluids in Bojungikgi-Tang treated group(1012${\pm}$248) was significantly decreased(p<0.05) compared with control group(1413${\pm}$346). 3. The concentration(pg/$m{\ell}$ of TNF-${\alpha}$ in peritoneal fluids in Bojungikgi-Tang treated group(887${\pm}$196) was significantly decreased(p<0.05) compared with control group(1126${\pm}$140). 4. Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatory cells and angiogenesis in transplanted uterine tissue of Bojungikgi-Tang treated group were weakly observed than those of control group. 5. The percentage of positive epithelial layers for COX-2 in Bojungikgi-Tang treated group(56${\pm}$17) was significantly decreased(p<0.05) compared with control group(75${\pm}$16). 6. The VEGF expression of endometriotic epithelia, neovascular endothelia and stromal cells in transplanted uterine tissue of Bojungikgi-Tang treated group were weakly observed than those of control group. Conclusion: On the basis of these results, we concluded that Bojungikgi-Tang has inhibiting effects on the development of transplanted uterine tissue. And these effects may be related with decreased production of MCP-1, TNF-${\alpha}$, and decreased expression of COX-2 and VEGF infiltration by administration of Bojungikgi-Tang.

• 한국식품영양과학회지
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• 제36권4호
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• pp.424-430
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• 2007

인동초를 이용하여 표고균사체를 접종, 배양하여 얻어진 인동초균사체 추출액의 간암세포, 유방암세포, 자궁경부암세포 그리고 고형암의 증식에 미치는 영향을 조사하였다. 3가지 암세포의 형태변화 및 증식 억제에 미치는 영향에서 간암세포와 유방암세포에서 암세포에 대한 특이적 형태변화가 관찰되었고, 특히 간암세포에서 인동초균사체 추출물이 인동초추출물보다 효과적인 것으로 나타났다. 인동초균사체를 간암세포에 3mg/mL로 처리했을 때 $85.60{\pm}4.66%$의 암세포 증식억제율을 나타내어 인동초를 처리한 것보다 암세포 증식 억제율이 61.10% 높았다. 유방암세포에서는 인동초 및 인동초균사체를 처리한 것 모두 높은 암세포 증식억제율을 보였으며, 두 군간의 차이는 미미한 것으로 나타났다. 자궁경부암세포에서는 인동초 및 인동초균사체를 처리한 것 모두 암세포 증식억제율이 미미한 것으로 나타났다. 고형암 억제효과에서는 대조군에 비해 인동초균사체 추출물에서 고형암이 61% 억제되었으며, 인동초균사체가 인동초추출물보다 고형암 억제효과가 30% 더 높은 것으로 나타났다. 히스타민 유리 억제효과를 측정한 결과 compound 48/80처리군에 비해 인동초추출물은 9.07%, 인동초균사체 추출물은 43.05%의 히스타민 분비 억제효과가 있는 것으로 나타났다.

• 한국식품영양과학회지
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• 제36권1호
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• pp.8-13
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• 2007

황기를 이용하여 황기균사체를 접종, 배양하여 얻어진 황기균사체 추출액의 간암세포, 유방암세포, 자궁경부암세포 그리고 고형암의 증식에 미치는 영향을 조사하였다. 3가지 암세포의 형태변화 및 증식억제에 미치는 영향은 황기균사체 추출물이 황기추출물보다 효과적인 것으로 나타났으며, 황기균사체를 간암세포에 3 mg/mL로 처리했을 때 65.23%, 유방암세포에 5 mg/mL로 처리했을 때 69.42%의 높은 암세포 증식 억제 효과가 있었다. 자궁경부암세포에서는 황기균사체를 3 mg/mL로 처리했을 때 황기를 처리했을 때보다 암세포 증식억제효과가 23.82%더 높았다. 특히 간암세포와 유방암세포에서 황기균사체 추출물을 처리하였을 때 강력한 암세포 증식억제효과가 있는 것으로 나타났다. 고형암 억제효과에서도 대조군에 비해 황기균사체 추출물이 47%의 고형암 억제효과가 있었고, 황기추출물보다 고형암 억제 효과 10% 더 높았다. 히스타민 유리 억제효과를 측정한 결과 compound 48/80 처리군에 비해 황기추출물은 7.6%, 황기균사체 추출물은 44.6%의 히스타민 분비 억제효과가 있는 것으로 나타났다. 이상의 결과로 볼 때 한방기능성 소재에 균사체 배양기법의 접목으로 효능의 상승과 복합기능을 기대할 수 있겠다.

• 대한한방소아과학회지
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• 제18권2호
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• pp.31-48
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• 2004

Objective : KagamJwagwiEum(KJE) has been used for the purpose of prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of KJE, the author examined cytotoxicity and inflammatory cytokines secretion with human leukemic mast cell line, HMC-1. Methods: HMC-1 was stimulated with phorbol 12-myristate 13-acetate(PMA) and calcium ionophore A23187. KJE by itself had no effect on viability of HMC-l. The effects of KJE on the secretion of tumor necrosis $factor-alpha(TNF-{\alpha})$, interleukin(IL)-6 and IL-8 from HMC-1 were evaluated with enzyme-linked immunosorbent assay. Results : KJE inhibited PMA plus A23187-induced $TNF-{\alpha}$ and IL-6 secretion. But KJE had no effect IL-8 secretion: KJE had immunoregulatory effects on cytokines, increased secretion of NO and $TNF-{\alpha}$ but did not effect IL-12 secretion when the cells were primed and trigged with $IFN-{\gamma}$ in the peritoneal macrophages of C57BL/6 mice. Conclusions : Taken together, these results suggest that KJE inhibit the production of inflammatory cytokines in HMC-1 cells and activate macrophages.

• 분석과학
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• 제29권4호
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• pp.186-193
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• 2016

본 연구는 용규전초로부터 열수추출방법(SNL-W)으로 1차 추출하고 부틸알코올을 이용하여 분별추출을 통해 유기용매분획(SNL-W/B)과 수상분획(SNL-W/W)을 각각 획득하였다. 각 분획의 총 알칼로이드함량과 사포닌함량을 측정하였고 수상분획의 메탄올 용출물은 알칼로이드 성분으로 확인되었다. 꿀벌봉독을 쥐의 복막 비만세포에 처리하여 알러지 유발물질인 히스타민을 분비시키고, 용규의 알칼로이드성분으로 히스타민 분비에 대한 저해효과를 고찰하였다. 그 결과, 용규의 수용성 알칼로이드성분(SNL-W/W/M)은 꿀벌봉독으로 유발된 비만세포의 히스타민 분비에 대하여 강한 저해요인으로 작용하는 것을 확인하였다.