• Journal of Veterinary Clinics
• /
• v.37 no.2
• /
• pp.61-66
• /
• 2020

Nickel is a nutritionally essential trace element that plays an important role in the immune system of several animal species. The aim of this study was to examine the effect of nickel chloride on chemotactic activity of peripheral blood polymorphonuclear cells (PMNs) and whether this effect is associated with interleukin (IL)-8 and a nuclear factor-kappa B (NF-κB)-dependent pathway. Peripheral blood mononuclear cells (PBMCs) and PMNs were isolated by Percoll solution (Specific gravity; 1.080) and 1.5% dextran treatment, respectively. A modified Boyden chamber assay was used to measure the chemotactic activity of PMNs. The level of IL-8 in culture supernatant from PBMCs was measured by enzyme-linked immunosorbent assay (ELISA). Both of PBMCs and PMNs exhibited a low viability when cultured with concentration of greater than 1,000 μM of nickel chloride for 24 h. Thus, nickel chloride was used at concentration of 500 μM, which preserved cell viability. Treatment with nickel did not directly affect the chemotactic activity of PMNs. However, the chemotactic activity of PMNs was remarkably increased by culture supernatant from PBMCs treated with nickel chloride (500 μM) for 24 h. Recombinant porcine IL-8 polyclonal antibody (pAb) neutralized the enhancing effect on the chemotactic activity of PMNs by culture supernatant from PBMCs treated with nickel and this culture supernatant had higher IL-8 levels than the culture supernatant from untreated PBMCs. In addition, n-tosyll-phenylalanine chloromethyl ketone (TPCK), a NF-κB inhibitor, antagonized the enhancing effect on the chemotactic activity of PMNs by the culture supernatant from PBMCs treated with nickel. These results suggested that nickel stimulates porcine PBMCs to produce IL-8, which increases the chemotaxis of PMNs via NF-κB-dependent pathway.

• Korean Journal of Microbiology
• /
• v.39 no.4
• /
• pp.235-241
• /
• 2003

We have previously demonstrated that intracellular expression of an RNA aptamer termed RRE40, which was selected in vitro to bind HIV Rev 10-fold much tighter than wild-type RRE, efficiently protected human CD4+ T cell line, CEM, from HIV-1. In this study, to evaluate the efficacy of the RRE40 RNA in clinical settings, polyclonal CD4+ peripheral blood lymphocytes (PBLs) were transduced with retroviral vectors expressing RRE40 decoy RNA and then challenged with clinical isolates of HIV-1. In contrast to the control cells transduced with vectors expressing control tRNA, intracellular expression of RRE40 RNA more effectively inhibited HIV-1 replication in CD4+ PBLs. However, transient and diminished inhibition, rather than complete inhibition, of HIV-1 replication in PBLs expressing RRE40 decoys have been observed. These results suggest that RRE40 decoy RNA would be useful to inhibit HIV-1 replication in cells. However, development of more efficient gene transfer protocols and/or more effective decoy RNAs would be needed to apply RNA decoy to modulate HIV-1 patient.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.30 no.3
• /
• pp.228-233
• /
• 2004

Summary: The malignant peripheral nerve sheath tumor(MPNST) is an aggressive neoplasm and can either arise independently or result from malignant change in preexisting neurofibromatosis (von Recklinghausen's disease). Its histologic characteristics remain controversial, but currently it is believed that the schwann cell is the origin of the peripheral nerve sheath tumors. MPNST is an uncommon neoplasm of the head and neck region, and its presentation in the oral cavity is quite rare. In this study, we report a patient with a rare case of a MPNST involving the maxilla. A case report: A 29-year-old female presented with a chief complaint of painless swelling with bleeding tendency on the left maxillary tuberosity area 2 months ago. Clinical examination showed a $5.0{\times}3.0cm^2$ sized, indurative swelling on the site. Conventional radiographs showed a relatively well-defined soft tissue mass involving the left maxillary sinus, and destruction of the anterior, posterolateral walls of the left maxillary sinus. Subtotal maxillectomy and split-thickness skin graft from thigh were undertaken. In histochemical and immunohistochemical studies, the specimen revealed positive reactivities to Vimentin and S-100 protein. Final diagnosis was made as MPNST.

• Journal of Veterinary Clinics
• /
• v.37 no.5
• /
• pp.249-254
• /
• 2020

Neutrophil extracellular trap (NET) formation is an immune response for the invasion of microbes. The purpose of this study is to examine the effect of zinc on NET formation of porcine peripheral blood polymorphonuclear cells (PMNs). The NET formation of PMNs was measured by fluorescence microplate reader. The production of tumor necrosis factor (TNF)-α in the culture supernatants from zinc-treated peripheral blood mononuclear cells (PBMCs) was measured by enzyme-linked immunosorbent assay (ELISA). Zinc itself did not have no effect on NET formation. However, the NET formation of PMNs was increased by culture supernatants from PBMCs treated with zinc. Also, the NET formation of PMNs was increased by recombinant porcine (rp) TNF-α. The production of TNF-α in PBMCs culture supernatants was shown to increase upon zinc treatments. These NET formations of PMNs increased by either culture supernatant from PBMCs treated with zinc or rpTNF-α were inhibited by treatment of anti-rpTNF-α polyclonal antibody (pAb). These results suggested that zinc has an immunostimulating effect on the NET formation of PMNs, which is mediated by TNF-α released from zinc-treated PBMCs. Therefore, zinc may play an important role for NET formation in the defense of porcine inflammatory diseases.

• The Journal of Korean Medicine
• /
• v.37 no.2
• /
• pp.45-52
• /
• 2016

Objectives: The purpose of this study is to evaluate the clinical effects of Muscle Energy Technique(MET) for peripheral facial paralysis. Methods: 60 Patients were divided into two groups. Group A(n=30) received the treatment with existing Korean medicine. Group B(n=30) received the MET with existing Korean medicine. It was performed once a day, five time per a week for three weeks. we analyzed Yanagihara's score and House-Brackmann scale Results: A week after MET treatment, Yanagihara's score average of Group A is $7.17{\pm}6.34$. Yanagihara's score average of Group B is $8.84{\pm}5.22$. (p=0.72). Two weeks after MET, Yanagihara's score average of Group A is $12.39{\pm}4.94$. Yanagihara's score average of Group B is $15.12{\pm}3.20$. (p=0.04). Three weeks after MET, Yanagihara's score average of Group A is $17.11{\pm}5.31$. Yanagihara's score average of Group B is $22.78{\pm}3.67$. (p=0.01). A is $3.87{\pm}1.36$. House-Brackmann Scale average of Group B is $3.64{\pm}1.76$. (p=0.63). Two weeks after MET treatment, House-Brackmann Scale average of Group A is $3.20{\pm}0.97$. House-Brackmann Scale average of Group B is $3.02{\pm}1.03$. (p=0.05). Three weeks after MET, House-Brackmann Scale average of Group A is $2.84{\pm}1.12$. House-Brackmann Scale average of Group B is $2.23{\pm}0.78$. (p=0.04). Conclusion: MET treatment is effective for improve the symptoms of peripheral facial paralysis. Therefore, it will be used to peripheral facial paralysis.

• The Korean Journal of Physiology
• /
• v.19 no.2
• /
• pp.113-125
• /
• 1985

Electrophysiological difference of the central and peripheral area of the sinoatrial node in the rabbit was studied by glass microelectrode technique. Effects of $K^+,\;Na^+,\;Cs^+,$ adrenaline and ouabain on the action potential of the two areas were investigated, and transient hyperpolarization ($K^+-induced$ hyperpolarization) which developed following readmission of potassium after having pre-treated with $K^+-free$ Tyrode solution for 10 minutes was analyzed. The results obtained were as follows ; 1) The frequency of the spontaneous action potential recorded in the periphery of the SA node was faster than the central area. Reduction by $Cs^+$ and increase by O mM $K^+$, $10^{-6}M$ adrenaline and $10^{-6}M$ ouabain in the frequency of action potential were noticed more prominently in the peripheral than the central area. On the contrary, the frequency in the central area was more decreased than the Peripheral area by 13 mH $K^+$ and 1 mM $Co^{2+}$. 2) The amplitude of the K+_induced hyperpolarization was very small in the central area but large in the peripheral area. Transient hyperpolarization was abolished by ouabain and low sodium, and decreased by cooling the tissue $(17^{\circ}C)$. 3) By changing the concentration of $Ca^{2+}$ in the perfusate, the amplitude and the rate of transient hyperpolarization were increased in the high $Ca^{2+}$ concentration. It could be concluded that the central area of the SA node is less susceptible to the inhibition of Na-Pump and more susceptible to Ca-blocker and high concentration of $K^+$. The Na-Pump activity of the central area measured by means of transient hyperpolarization is found to be much less active than that of the peripheral area.

• Journal of Acupuncture Research
• /
• v.29 no.3
• /
• pp.121-128
• /
• 2012

Objectives : The aim of this study was to observe the effect of Pyung-Hyung acupuncture on peripheral facial palsy. Methods : We investigated 44 cases of patients with peripheral facial paralysis, and divided patients into two groups. We treated one group by complex oriental medical treatment with Pyung-Hyung acupuncture, and the other group by complex oriental medical treatment without Pyung-Hyung acupuncture. To evaluate the effectiveness of treatment applied for two groups, we used Yanagihara's unweighed grading system(Y system) and gross grading system of House-Brackmann(HB score) at baseline and final. Results : 1. The final Y-system in both the Pyung-Hyung acupuncture group and the control group significantly increased compared to the value at baseline. 2. At final, Pyung-Hyung acupuncture group showed significantly increase on Y-system compared with control group. Conclusions : Pyung-Hyung acupuncture can be used for relieving symptoms related with peripheral facial paralysis.

• Tuberculosis and Respiratory Diseases
• /
• v.64 no.5
• /
• pp.379-382
• /
• 2008

Eosinophilic pleural effusions (EPE) are defined as those effusions that contain at least 10% eosinophils, and EPE can be associated with peripheral blood eosinophilia in a variety of systemic diseases. There have been a few cases that have addressed the association of peripheral blood eosinophilia and posttraumatic EPE, and this condition can be misdiagnosed as being the result of other causes due to the delayed presentation. We report here on a case of 47-year-old male who presented with eosinophilic pleural effusion associated with peripheral blood eosinophilia at 2 months after minor chest trauma. We excluded the other possible causes such as consumption of drugs, parasite infection, malignancy, diseases of pulmonary eosinophilic infiltration, autoimmune diseases and pulmonary thromboembolism. We observed his clinical course without specific treatment. Three months later, the pleural effusion completely disappeared and the number of peripheral eosinophils returned to normal.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.30 no.4A
• /
• pp.345-349
• /
• 2005

In this paper, the enhanced ATA(AT Attachment Interface with Extension) interface that combines the price of ATA and intelligent operation of SCSI(Small Computer System Interface) is realized both performance and structure of general interface used to connect peripheral devices that are used in PC(Personal Computer). SCSI bus is for peripheral devices that have intelligent operation. It is excellent bus that has well-arranged command system and stable hardware structure. But is aspect of price this peripheral device has the difficulty in purchase because there is price difference more than two times compared with ATA peripheral device. Whereas, although ATA peripheral device had low performance in the early period, after the improvement its speed has almost etched to SCSI and its price is also excellent. In this paper, the enhanced interface structure to fulfill price and performance was suggested and materialized as the hardware using such two advantages.

• Journal of Acupuncture Research
• /
• v.19 no.1
• /
• pp.11-23
• /
• 2002

Objective : Lately oriental medicine treatment of peripheral facial paralysis is various. In various treatments, this study makes a report that effect of our clinic using aqua-acupuncture with hominis placenta for peripheral facial paralysis. Materials and Methods : This report have been observed among twenty-six patients with peripheral facial paralysis who admitted to oriental medicine hospital with Se-myung university during Jan. 1st, 2001 to Oct. fourth, 2001. These patients were divided into two groups; the first group added aqua-acupuncture with hominis placenta to basic oriental medicine treatment., while the second group was only treated with basic oriental medicine treatment. Results : The results of treatment with adding aqua-acupuncture with hominis placenta to basic oriental medicine treatment showed that 9 of 12(75%) patients achieved good or more recovery. While the second group was observed that 6 of 14(50%) patients achieved good or more recovery. Conclusion : The outcome of the observation, we concludes that the therapeutic rate of the group of adding aqua-acupuncture with hominis placenta was higher than that of basic oriental medicine treatment.