• Parasites, Hosts and Diseases
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• 제21권2호
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• pp.150-156
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• 1983

1975년부터 1983년까지 치료를 위해 서울의대 기생충학교실로 의뢰된 광절열두조충 충란양성자 5명에 대하여 bithionol, niclosamide 또는 praziquantel을 투여 하고 하제를 사용하여 충체를 얻은바 Diphyllobothrium latum으로 동정되었다. 충자는 서울 및 전남 완도군에 거주하고 있는 10~64세 남(4명) 여(1명)로 그중 2례는 농어를 생식한 경험이 있으며 나머지 3례는 바다생선회를 생식하였으나 그 종류를 기억할 수 없다고 한다. 또 그중 3례는 심한 복통과 소화기증상을 경험하였고, 2례는 편절이 자연배출된 일이 있다고 하나 빈혈소견은 전례에서 관찰되지 않았다. 대편검사에서 발견된 충란은 명 열별로 크기 평균 $59~67{\times} 41~48{\mu}m$이고 황갈색 유란원형 또는 타원형이 있으며 operculum과 abopercular protuberance가 뚜렷하였다. 충체는 4례에서 두절을 포함한 걸이 320~680cm의 완전한 것이 배출되었고 l례에서는 투약후 충체 배출여부를 확인하지 못하고 자연배출된 길이 30cm정도의 체절을 동정에 이용하였다. 특징적인 편절의 형태인 rosette형 자궁, vaginal pore가 cirrus sac에 포함되어 개구된 점, 재정과 cirrus sac의 분리 등으로 보아 Diphyllobothrium latum으로 동정되었다. 본 5례는 우리나라에서 1971년 이후 충체가 확인된 6례에 이어 제 7례-제11례의 인체기생례가 된다.

• Parasites, Hosts and Diseases
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• 제55권6호
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• pp.631-641
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• 2017

A survey was performed to investigate the infection status of zoonotic trematode (ZT) metacercariae in fish from a local market in Yangon City, Myanmar. A total of 264 fish (12 species) were collected through 4 times from December 2013 to June 2015. All collected fish were transferred to our laboratory on ice and examined by the artificial digestion method. More than 7 species of ZT metacercariae, i.e., Haplorchis taichui, H. pumilio, H. yokogawai, Centrocestus spp., Stellantchasmus falcatus, Pygidiopsis cambodiensis, and Procerovum sp. were detected. Metacercariae of H. taichui were collected in 58 (42.3%) out of 137 fish (5 species), and their average density was 42.9 per fish infected. Metacercariae of H. pumilio were detected in 96 (49.0%) out of 196 fish (9 species), and their average density was 23.6 per fish infected. H. yokogawai metacercariae were found in 40 (50.0%) out of 80 fish (5 species), and Centrocestus spp. metacercariae in 91 (50.8%) out of 179 fish (8 species), and their densities were 306 and 25.8 per fish infected, respectively. Metacercariae of S. falcatus and P. cambodiensis were detected only in mullets, Chelon macrolepis. A total of 280 Procerovum sp. metacercariae were found in 6 out of 12 climbing perch, Anabas testudineus. Morphological characteristics of adult flukes recovered from experimental animals were described. It has been first confirmed that fish from Yangon, Myanmar are commonly infected with various species of ZT metacercariae.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.113-118
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• 1999

The insulin-like growth factor (IGF) is found in all vertebrates and its type-II molecule is regarded as a fundamental embryonic growth factor during development. We have firstly identified, in this study, a cDNA clone corresponding to IGF-II (flIGF-II) from the adult brain of the teleost, Paralichthys olivaceus. We also examined the tissue expression of flIGF-II in several adult tissues by RT-PCR. The flIGF-II cDNA contained a complete ORF consisting of 215 amino acids and one stop codon. Its molecular characteristics appear to be similar to the previously identified IGF-II molecules, in which a common primary structure exhibiting B, C, A, D, and E domains is evidently observed. This cDNA clone seems to be cleaved at $Ala_{52}$ for the $NH_2$-end signal peptide and appears to produce a 98 amino acid-long E-peptide from the $Arg^{118}$. The functional B-D domain regions, therefore, include 65 amino acids and is able to encode a 7.4-kDa protein. The most prominent structural difference between IGF-I and IGF-II was that the D domain of IGF-II exhibits a two-codon-deleted pattern compared to the 8 amino acid-containing IGF-I. The insulin family signature in the A domain and six cysteins forming three disulfide bridges between the B and A domains were evolutionary-conserved from teleosts to mammalian IGF-II. Interestingly, the E-peptide region appears to provide a distinct hallmark between teleosts in amino acid composition. The flIGF-II shows 85.1% of sequence identity to salmon and trout, 90.6% to tilapia, and 98.4% to perch in amino acid level. In tissue expressions of IGF-II, it is very likely that flIGF-II has a significant expression in the adult brain. However, liver seems to be the main source for IGF-II production, and relatively low signals were observed in the adult muscle and kidney. Taken together, it would be concluded that the functional region for IGF-II mRNA is highly similar in phylogeny and is evolutionary, conserved as a mediator for the growth of vertebrates.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1230-1236
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• 2012

Fish meal grades SL1 and SL2 from Sardine (Sardinella longiceps) and NJ from Pink Perch (Nemipterus japonicas) were evaluated as a sole source of carbon and nitrogen in the medium for alkaline protease production by Bacillus pumilus MTCC 7514. The analysis of the fish meal suggests that the carbon and nitrogen contents in fish meal are sufficient to justify its choice as replacement for other nutrients. Protease production increased significantly (4,914 U/ml) in medium containing only fish meal, compared with the basal medium (2,646 U/ml). However, the elimination of inorganic salts from media reduced the protease productivity. In addition, all the three grades of fish meal yielded almost the same amounts of protease when employed as the sole source of carbon and nitrogen. Nevertheless, the best results were observed in fish meal SL1 medium. Furthermore, protease production was enhanced to 6,966 U/ml and 7,047 U/ml on scaling up from flask (4,914 U/ml) to 3.7 and 20 L fermenters, respectively, using fish meal (10 g/l). Similarly, the corresponding improvement in productivities over flask (102.38 U/ml/h) was 193.5 and 195.75 U/ml/h in 3.7 and 20 L fermenters, respectively. The crude protease was found to have dehairing ability in leather processing, which is bound to have great environmental benefits.

• Parasites, Hosts and Diseases
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• 제45권3호
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• pp.219-223
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• 2007

A case of Diphyllobothrium latum infection in a 49-year old man is described, and diphyllobothriasis latum in the Republic of Korea is briefly reviewed. An incomplete strobila of a tapeworm, 95 cm in length, without scolex and neck, was spontaneously discharged in the feces of a patient. On the basis of morphologic characteristics of the worm and eggs, the worm was identified as D. latum. The patient was successfully treated with a single dose (15 mg/kg) of praziquantel. The most probable source of infection was salmon flesh according to the past history of the patient. The first case of D. latum infection was documented in 1971, and this is the 43rd recorded case in the Republic of Korea. The 43 cases were briefly reviewed. The patients' main complaints were gastrointestinal troubles, such as mild abdominal pain, indigestion, and diarrhea, and discharge of tapeworm segments in the feces. The suspected infection sources included raw or improperly cooked flesh of fresh or brackish water fish, including the perch, mullet, salmon, and trout.

• ALGAE
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• 제31권1호
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• pp.17-31
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• 2016

To explore the feeding ecology of the newly described heterotrophic dinoflagellate Aduncodinium glandula in the family Pfiesteriaceae, its feeding behavior and prey species were investigated. Additionally, the growth and ingestion rates of A. glandula on the mixotrophic dinoflagellates Heterocapsa triquetra and Akashiwo sanguinea, its optimal and suboptimal prey, respectively were measured. A. glandula fed on prey through a peduncle after anchoring to the prey using a tow filament. A. glandula ate all algal prey and perch blood cells tested and had the most diverse prey species in the family Pfiesteriaceae. Unlike for other pfiesteriacean species, H. triquetra and A. sanguinea support the positive growth of A. glandula. However, the cryptophytes Rhodomonas salina and Teleaulax sp. and the phototrophic dinoflagellate Amphidinium carterae did not support the positive growth of A. glandula. Thus, A. glandula may have a unique kind of prey and its optimal prey differs from that of the other pfiesteriacean dinoflagellates. With increasing mean prey concentration, the growth rates of A. glandula on H. triquetra and A. sanguinea increased rapidly and then slowed or became saturated. The maximum growth rates when feeding on H. triquetra and A. sanguinea were 1.004 and 0.567 d⁻¹, respectively. Further, the maximum ingestion rates of A. glandula on H. triquetra and A. sanguinea were 0.75 and 1.38 ng C predator⁻¹ d⁻¹, respectively. There is no other pfiesteriacean species having H. triquetra and A. sanguinea as optimal and suboptimal prey. Thus, A. glandula may be abundant during blooms dominated by these species not preferred by the other pfiesteriacean dinoflagellates.

• 한국수산과학회지
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• 제51권2호
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• pp.170-177
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• 2018

Samples were obtained from broodstork in May, 1998, while naturally fertilized embryos were maintained and the process of skeletal development was observed from larvae and juveniles. Prelarvae immediately after hatching showed an average total length of $5.38{\pm}0.41mm$ (n=10), premaxillary and dentary were ossified, parasphenoid was ossified in the cranium, and centrum and caudal bone did not ossify. Prelarvae showed ossification with maxillary, articular and epihyal and branchiostegal rays of hyoid arch were ossified at 5 days after hatching with an average total length of $6.40{\pm}0.39mm$ (n=10). The vertebrae began to ossify in the direction of the tail, and neural spine began to ossify above the ossified vertebra. Postlarvae showed ossification of lateral ethmoid, parietal, and caudal skeleton in the cranium when the average total length was $7.30{\pm}0.12mm$ (n=10) in 8 days after hatching. At 22 days after hatching, postlarvae ossified maxillary in the cranium, and ossified endopterygoid and ectopterygoid, etc. in the palate, when the average length of $11.1{\pm}1.27mm$ (n=10). At 32 days after hatching, with the average length was $12.8{\pm}1.97mm$ (n=10), caudal skeleton had one additional epural bone ossification, resulting in ossification of a total of 3 epural bone to complete ossification of all spicules.

• 대한수의학회지
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• 제30권4호
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• pp.479-485
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• 1990

This study was carried out to observe in vitro effect of praziquantel on the viability and internal organ changes of Heterophyopsis continua with light microscopy. Metacercariae were collected from the perch, Lateolabrax japonicus, by artificial digestion technique and fed to 2-week old chickens. Adult worms were recovered from the small intestines of chickens 8 days after infection. For working solutions, praziquantel was diluted with TC199 medium at the concentration of 0.01, 0.1, 1 and $10{\mu}g/ml$. To each petri dish containing 10ml of solution, 5~10 worms were introduced and incubated at $37^{\circ}C$. Motlity of worms was observed at 5, 15, 30, 60 minutes, 1, 2, 4 and 6 hours after incubation. For light microscopy, worms were fixed in 10% formalin under cover glass pressure and stained with Semichon's acetocarmine. The results were as follows: 1. In $0.01{\mu}g/ml$ praziquantel, the worms had their mobility until 6 hours post treatment. However, worms in over $0.1{\mu}g/ml$ of praziquantel contracted within 5 minutes and immobilized. 2. Intestine of the worm incubated in $0.001{\mu}g/ml$ praziquantel for 5 minutes was dilated and intestinal wall was thickened. 3. In incubated over $0.1{\mu}g/ml$ praziquantel, pharynx of the worm protruded out from oral sucker. 4. The lowest effective lethal concentration of praziquantel on H. continua was $0.1{\mu}g/ml$. The worms exposed to the drug were observed to be immobilized immediately after incubation in solutions of over $0.1{\mu}g/ml$ concentration. All of the worms in early period showed severe contraction and those in late period showed severe dilation.

• Restorative Dentistry and Endodontics
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• 제24권2호
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• pp.286-298
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• 1999

The endodontic-periodontic combined lesions have been difficult to get correct diagnosis and predictable treatment. This study was to make the experimental endodontic-periodontic combined defects in dogs for the study of the periodontal regeneration and to evaluate the efficacy of the enamel matrix protein and e-PTFE membrane in the experimental endodontic-periodontic combined defects. 5 mongrel dogs were used. The pulp chambers were opened and the plaque was inserted into the chambers to induce the periapical lesions on the mandibular second, third and fourth premolars of the dogs. 1 month later, the root canal treatments were done with gutta perch a and ZOE sealer. On the day of surgery, the periapical defects were standardized by trephine bur. The buccal dehiscence defects were made by the dental bur and bone chisels. The apicoectomy with retrofilling was done. The prepared roots were randomly selected for test and control groups. In the experimental groups, the enamel matrix derivative and e-PTFE membrane were used. Nothing was placed on the control group. Fluroscent labelling was used to evaluate the bone formation. After 4 and 12 weeks, the dogs were sacrificed and undecalcified sections were prepared and stained with toluidine blue. Those histologic sections were examined by fluorescent microscopy and light microscopy. The results were as follows. 1. In the control group, new bone was formed in the periapical defects and scarcely in the buccal dehiscence defects. New cementum was not detected at 4 and 12 weeks. 2. In the experimental groups, new bone, new cementum and periodontal ligament were found in the periapical and buccal dehiscence defects. The relative amount and the quality of the new bone, new cementum and periodontal ligament tissue that had formed on the experimental groups were superior to those of the control group. 3. The current observation implicated that e-PTFE membrane and enamel matrix protein could be the effective tools for the guided tissue regeneration of the endo-perio combined defects.

• 생태와환경
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• 제35권3호통권99호
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• pp.232-236
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• 2002

한국산 꺽지 번식에 대한 연구의 일환으로 정소 및 정자의 형태적 특징을 조사하였다. 2001년 5월부터 10월에 걸쳐 북한강 지류인 수입천 지역에 서식하는 꺽지(Corecperca　herzi)를 채집하여 분석하였다. 정소는 흰색을 띄며 쐐기모양을 하고 복강의 등쪽에 좌우 균등하게 발달하였다. 동일한 정자형성 단계에 있는 생식세포들이소낭세포에 싸여 발달하며 여러 개가 모여 소엽구조를　형성한다. 각 소엽은 여러 개의 소낭들로 구성되며 소낭들은 각각 속에 들어 있는 생식세포의 정자형성 단계가　상이하였다. 정자는 길이는 43 ${\mu}$m로 둥근 형태의 두부는 2.2 ${\mu}$m 길이이며 0.5 ${\mu}$m길이의 중편이 두부 밑에 발달한다. 미부의 길이는 40 ${\mu}$m이다. omassie brilliant blue염색 결과 정자의 두부 선단에 강한 염색을 확인하였는데 첨체를 발달시키는 것으로 사료된다.