• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.297-302
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• 2009

This study was carried out to investigate the characteristics of rooting and bud break of single-stemmed roses (Rosa hybrida L. 'Rate Rose' and 'Teresa') as affected by axillary bud position and leaf area of cuttings derived from different growth stage of the mother shoots. In fact, both rooting and budbreak of single-stemmed roses were not influenced by growth stage of mother shoots, with showing more than 95% in all treatment related to mother shoot maturity. 'Rote Rose' required 34 days to average rooting and budbreak after cutting, whereas 'Teresa' did only 18 days to budbreak which was 9 days faster than rooting. Rooting and bud break needed more time and showed lower percent as the axillaty bud position for cuttings went down to the base of mother shoots. Especially 'Teresa' showed 12 days of delay to budbreak and 14.4% decrease in budbreak. Also, the increase in leaf area of cuttings accelerated rooting and budbreak, of which the time was shorter and the percent was higher.

• Journal of Bio-Environment Control
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• v.23 no.1
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• pp.31-38
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• 2014

Rooting agents and shading treatments were tested in two different experiments to determine their effects on the establishment success of hardwood cuttings of three highbush blueberry varieties 'Bluecrop', 'Duke' and 'Sunrise'. For the experiment with rooting agents, one-cm long bases of the cuttings were dipped into solutions of IBA or NAA for 5 s, both at 0, 500 and $1000mg{\cdot}L^{-1}$ in 50% ethanol, and were also treated with Rootone$^{(R)}$ powder. Determined 90 days after cutting, the percent rooting and root weight were increased by NAA at $500mg{\cdot}L^{-1}$ in 'Bluecrop' and 'Sunrise', while in 'Duke' IBA at $500mg{\cdot}L^{-1}$ was effective. These auxin treatments were found to work better than a commercial product Rootone$^{(R)}$. The rooting agent-induced increases in root development resulted in better shoot growth of the cuttings in all three varieties, as determined after 90 days of further growth in individual containers. In the experiment with shading treatments, different levels of the shading treatment (30 to 90%) were imposed over the cutting bed under no mist. In all three varieties, 30% shading increased the percent rooting and root and shoot growth, compared to no shading control. However, shading levels higher than 50% shading were found to be inhibitory for hardwood cuttings of highbush blueberries, especially under the environmental conditions with no mist system.

• Journal of Korean Society of Forest Science
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• v.1 no.1
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• pp.12-21
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• 1962

Outdoor cuttings studies on two coniferous species, pitch pine and needle fir, have been made at Suwon, Korea extending from March to early May, 1956. The purpose of these studies was to find the effects of ages of parent trees, topophysis, dilute hormone solution treatments and the time at which the cuttings were collected on the rooting response of the cuttings. On September 2, rooting results were examined. Pitch Pine (Pinus rigida) The total of 1335 cuttings were collected from 3- and 10-year old trees. The cuttings treated were planted on March 13 and April 20. 1. In the cuttings collected from 3-year-old seedlings, the best result (34 percent rooted) was obtained by treating ends of cuttings with a mixture of 50 ppm IBA and 50 ppm NAA. 2. No rooting was observed in lammas shoots. 3. The cuttings from 3-year-old seedlings gave better rooting than those from 10-year-old trees. 4. The root origins were developed from the lower part of the cutting and the cut surface. Needle fir (Abies holophylla) One hundred twenty-five cuttings were collected from a 30-year-old tree. The cuttings treated with any one of four mixed hormone solutions were planted on March 23. 1. Fifteen cuttings out of 75 cuttings from the lower half of the crown were rooted. The cuttings obtained from the upper half of the crown did not root at all. 2. The cuttings treated by the hormone solution made up of 20 ppm IAA, 20 ppm IBA, 20 ppm NAA, 20 ppm thiamine and 5 percent sucrose gave the best rooting (22 percent). 3. All roots originated from callus tissues and irregularly arranged parenchymatous tissues near the cut base.

• Journal of Forest and Environmental Science
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• v.25 no.2
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• pp.119-126
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• 2009

An efficient in vitro plant regeneration system was developed through shoot proliferation from axillary buds of Tectona grandis (L.f), APNBV-1 (Andhra Pradesh North Badrachalam Venkatapuram-1) clone. Multiple shoots of high quality were produced in vitro from axillary bud explants. An average of 4.39 shoots/explant were obtained on Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) benzyl amino purine (BA), kinetin (KN), indole acetic acid (IAA), gibberillic acid ($GA_3$), growth adjuvants casein hydrolysate (CH), adenine sulphate (Ads) and antioxidants ascorbic acid, polyvinyl pyrrollidine (PVP). Eighty five percent of rooting was observed in ex vitro rooting media containing IBA and vermiculite. In ex vitro rooting, single shoots with 2 to 3 nodes were subjected to IBA of different concentrations at different periods of time intervals. Direct rooting in vermiculite at 500 ppm concentration of IBA resulted in 4.3 number of roots with 2 cm length. Minimum response of rooting and length of roots were recorded at 100 ppm concentration of IBA. Planlets were transferred to plastic bags for short acclimatization stage in green house where they survived at 95%.

• Journal of Korean Society of Forest Science
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• v.70 no.1
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• pp.1-6
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• 1985

The purpose of this experiment lies in finding the most appropriate cutting condition of Taxus cuspidata Sieb. et Zucc. which has been considered valuable tree species in gardening. Statistical analysis was focused on the comparison of the average ratios of rooting between greenhouses and fields, based on the observation rooted autogenis. To conduct this survey, hardwood cutting of Taxus cuspidata Sieb. et Zucc. were performed at both places of experiment above on April 20, 1982 with rooting results calculated on October 31, 1982. After formation of cuttings, it was soaked in IBA 200 ppm for 12 hours and than put in a bed. A summary of the result is presented as follows; 1) Cutting at the greenhouse showed higher average rate of rooting than at fields. Same tendency was found regardless of the differences in treatment. 2) It was clear that IBA-treatment contributed remarkably to causing higher average rooting rate. As an evidence, when twenty centimeter cuttings were inserted sandy media, IBA-treated area resulted in eighty six percent of average rooting rate, as compared with only twenty three percent in nontreatment case. In case of field cutting, IBA-treatment brought about fifty three percent of rooting in comparison with eleven percent nontreatment. 3) When sandy soil, loam and brown soil were separately used as cutting media, the highest rooting rate was found in case of sandy soil, without any difference between the two experimental places above. 4) As a result of the analysis to seek the impact of the length of cuttings on rooting, the range of length form fifteen to twenty centimeter was apparently most appropriate. It was also found that the rate of rooting declined beyond twenty five centimeter. 5) Two kinds of rooting pattern were observed. One was the case that callus cell lump was created on the lower cut side of cuttings. Importantly, root radical were formed inside the lump to influence the germination of root system. The other relates to the case that adventitious root which look like lateral roots appeared at the stem region. In abstract, first, sandy soil was effectively recommended in case of hardwood cutting in April. Second, the most appropriate length of cuttings ranged between fifteen and twenty centimeters. Third, high density IBA treatment was clearly effective. Forth, for proper environmental management, both pre-disinfection of sail by sterilizer and maintenance of high relative humidity were essentially required.

• Korean Journal of Environment and Ecology
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• v.29 no.6
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• pp.842-849
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• 2015

This study investigated the effects of rare-earth fertilizer on the shoot cuttings' rooting of Vitex rotundifolia L. and Tamarix chinensis Lour. The shoot cutting test was carried in 2008 and the main results are summarized as follows. The rate of rooting and the average roots increased in both number and length when rare-earth fertilizer is treated in V. rotundifolia and T. chinensis in comparison to those of the untreated control plot. In particular, when rare-earth fertilizer is diluted with water 1/2500, the rooting outstandingly increases. This result is almost similar to the effect of the rooting stimulant, IAA. Although there is no differentiation in its rooting rate according to the density, the rooting of T. chinensis shows a 100 percent effect on in the entire treated plot but not in the untreated control plot, so it is usable as a rooting stimulant. As for shoot cuttings' rooting, depending on the time immersed in diluted solution of rare-earth fertilizer, both V. rotundifolia and T. chinensis showed relatively higher percentages in all treatment plot immersed for 60 minutes than for 10 minutes. In conclusion, considering the results of the rooting percentage and the average number and length of roots of V. rotundifolia and T. chinensis, the shoot cuttings' rooting appeared higher in percentage when they were immersed in the rooting stimulant for sixty minutes with a lower density than 1/2500. This result shows that rare-earth fertilizer can be utilized as an alterative for IAA rooting stimulants currently available in the market.

• The Journal of Natural Sciences
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• v.1
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• pp.115-198
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• 1987

The present studies were undertaken to elucidate the influence of auxins, auxin-like substance-ethychlozate ("Figaron"),and pH and sort of rooting media on rooted propagation of certainornamental woody plant cuttings, and to see possible changes in internal compositions characterizing after root-promoting treatment as the cutting stage proceeded. The experimental check-up srevealed and summarized as seen in the following;I. Effect of three different auxin treatments on rooting cuttings: 1) Promotive influence of auxin varied according to different concentration levels, hours of dipping treatment of the auxins, and kind of plants. The greatest effect was obtained for Forsythia ksreana with NAA and IBA, for Ligustrurn obtusifolium var. variegatum with NAA and ethychlozate, for Hydrangea macrophylla, Magnolia kobus, and Magnolia liliflora with NAA, lBA and ethychlozate also. The most effective level of the promotive agents was found 200mg/l for NAA, 1000mg/l for IBA, and 200mg/l for ethychlozate. For Weigela florida and Gardenia jasminoides, range of the most effective level was shown relatively wide spread. 2) NAA was more effective at its optimal level of the rooting agent than ethychiozate for Weigela florida, Viburnum awabuki, Forsythia koreana, Acer palmatum 'Nomura', Bouga invillea glabra, Elaeagnus umbellata, Prunus tomentosa, Ligustrum obtusifolium, Pyracantha coccinea, Cestrum noctu rnum, Hydrangea macrophylla, Codiaeum variegatum, Rhododen dron lateritium, and Ilex crenata var. macrophylla, and yet ethychlozate was found either as equally as effective or more so than NAA for Zebrina pendula, Hibiscus syriacus, Fatshedera lizei, Schefflera arboricola, Campsis grandiflo ra, Ixora chinensis, Euonymus japonica, and Magnolia liliflora. On the contrary, no the auxin effect was noted with Lagerstroemia indica, Trachelospermum asiaticum, and Syringa vulgaris. This probably indicates that these species are genetically different for the auxin response.II. Effect of different pH and sorts of cutting media on rooting cuttings: 1) Bougainvillea showed best in rooting for the number and dry weight at pH 6.5, more with ethychlozate than NAA, while Ligustrum did at pH 5.0 more with NAA than ethychlozate. pH 4.0 medium resulted in the best rooting for Rhododendron with NAA, more than ethychlozate. 2) Use of cutting medium with peat: perlite: vermiculite = 1:1:1 showed to give the greatest rooting percent and dry weight, apart from considering the number of roots. This apparently meant the fact that cutting medium has more to do with root growth than root differentiation. Rhododendron yet showed results with cutting media that use of peat: perlite = 2:1 mixed is more effective on rooting than using peat alone.III. Effect of auxinic treatments on rooting cuttings and change in some cutting compositions: 1) Under the climatic conditions of July having temperature $26.3\pm$$2.4^{\circ}C$for cutting bed, new roots of Magnolia started to show up generally 20 days after the cutting was made, whereas Cestrum did much earlier than that, namely 14 days after. 2) Although total carbohydrate content of Magnolia cuttings showed no marked change without auxin treatment, it did so with the treatment, especially 30 days after the start of cutting. Cestrum cuttings demonstrated a gradual in crease in total carbohydrate content as rooting took place, and the content became reduced more with auxin than with out, just about when rooting proceeded to 14 days after the start of cutting. 3) Magnolia generally showed an increase in total nitrogen content as rooting proceeded more, and Cestrum showed a decrease in total nitrogen of cuttings. The auxin treatment exhibited no pertinent relation with change in plant nitro gen when rooting is promoted with auxin treatment. 4) An abrupt drop of total sugar and reducing sugar was noticed as Magnolia rooting started, and this reduction was parti cularly outstanding with auxin treatment. Starch content also was decreased in the later stage of cutting with auxin treatment, and was rather increased without auxin. Although sugar content soon increased as cutting started with auxin treatment in the case of Cestrum, it became reduced after rooting took place. 5) Total phenol content increased with rooting, and this was especially true when rooting started. This increase was reversed somehow regardless of auxin treatment. A decrease in phenol of Magnolia was found more striking with auxin than without in the later stage of the cutting period. 6)Avena coleoptile test for auxin-like substances presented the physiologically active factor is more in easy-to-root Magnolia liliflora than hard-to-root Magnolia kobus, and the activity of auxin-like substances was much increased with auxin treatment. The increase in the growth promoting substances was markedly pronounced when rooting just started. The active growth substances decreased in the later stage of cutting, and certain inhibitory substances started appearing. Cestrum also showed physiologically similar growth promoting substances accompanying auxin-like active substances if auxin is treated, and some strong inhibitory substances seemed to appear in the later stage of cutting. 7) Mung-bean-rooting test indicated biologically that endogenous growth substances in Magnolia all promoted mung-bean rooting, and activity of the growth substances apparently stimulated mung-bean rooting with auxin more than without. Here auxin treatment seemed to give a rise to an increased activity of endogenous growth substances in cuttings. This activity was found much greater with either NAA or IBA than ethychlozate, and showed its peak of the activity when rooting first started taking place. Certain inhibitory substances for Avena coleoptile growth strongly promoted mung-bean rooting, and it was also much like in the case of Cestrum.

• Korean Journal of Medicinal Crop Science
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• v.7 no.3
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• pp.200-204
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• 1999

Ligusticum chuanxiong has been an important oriental crude drug and cultivated for a long time in Korea. Ligusticum chuanxiong has been vegetatively propagating crop without seed due to its sterility and would be an allodiploid derived from the hybridization of two allied species. Generally, vegetative propagation is more costly per unit propagule than sexual(seedling) propagation. This study was conducted to find out the techniques of mass propagation by stem cutting for reduced seedling cost. When cutted on August 30 with 1st to 2nd internode, its highest rooting percentage was 81 percent. Rhizome yield of plants cutted in 50 percent on September 1 decreased to 9 percent less than control (rhizome propagation). Rooting percentage of rooted cutting plants after field planting on October 25 was the highest being 87 percent. Rhizome yield with planting appeared 127 and 120kg/10a on September 25 and October 25, respectively.

• Asian Journal of Turfgrass Science
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• v.9 no.1
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• pp.1-10
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• 1995

This study was carried out to investigate the method of propagation of Lindera erythrocarpa Makino. The results were as follows: 1. After the treatment of warm stratification for 1 month followed by cold stratification for 3 months, the germination ratio of seeds of Lindera erythrocarpa was from 84.6 to 93.6 percent. But, dry stored seeds lose their viability Quickly and should be placed in pretreatment im-mediately. Also, the seeds of control district germinated from 68.6 to 74.6 percent. So it was confirmed that the seed of Lindera erythocaeicarpa was not doubly dormant. 2. Softwood cuttings of Lindera erythrocarpa with 5,000 ppm IBA on August 4. 1992 resulted in the best rooting of 95.0 percentage. 3.Twenty percent of the graft union were obtained in the green wood grafting in Lindera erythrocarpa on June 7, 1993. And five percent of the graft union were obtained in the hard wood grafting in Lindera erythrcoar pa on April 6. 1993.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.3 no.3
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• pp.8-11
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• 2000

The experiment was carried out in order to the effects of NAA on rooting of stem cutting of leaf variegated Aster glehni, dwarf type of Achillea sibirica and Solidago virga-aurea var. asiatica native to Korea. Higher rooting percentage(85%) of Aster glehni, promoted root number and root length of Achillea sibirica were obtained by basal dip treatment with $1,000mg{\cdot}L^{-1}$ NAA for 3 hours on late June. Percent rooting of the Solidago was not enhanced, but root number and root length were promoted by basal dip treatment with $100mg{\cdot}L^{-1}$ NAA.